NM_181421.4;A130010L24; EG545802; Gm15800; mKIAA0614
100μg liquid plasmid(Instantly):$265
| name | pLV3-CMV-Hectd4(mouse)-3×FLAG-CopGFP-Puro | another name | apLV3-CMV-Hectd4(mouse)-3×FLAG-CopGFP-Puro |
| Insert Size (bp) | 13257bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGG GCTCGTCGGC CGCGGCGGCG GCAGCTGCAG CGGCGGCGGC GGCGGCGGCG GACTCGGCGC AGTGGCTCTC GGTGAAGGAG GAGACCATCT TTCTGCACGA CGGACTCATC CGGGTCACCG ACCTGGCCGA GCTGCCCAGC GAGATCCTCG GCGCCCCGGA GGCCGCCGAC ACCGACCTGG AGATTTTAAC ATTCGAGACC AAGAACCCAA CAGAATTAGC AGAACGTTTG CGCTCTGTTT GTGGGAACCA GAGCAATGCC TATGCCCGCC TGCTGGAATA CCGCCTGAAT GCCTTGCGTG GACTATGGAA TGCCCAGCGC CAGCTGGCCC TGGAGGAGCA ACATGAAAGG GAAGGCTCAG GTGACGAAGA GACCTTGGCC CTGCTCAAGC GCCAGGGCTT GTTGCAGCAG CCTGAACAAG CCCCCTTCAC GTCCCGGGTG GGCCTCCTGT TGGTCTTCCC CCTTATTCAG TCCCAGAGTA GAACAGACCC TTCCCTCTGT AACATAACTG CTGAGGTGCT ATTAAACTGC CTGCGAGACT GTCAGCCTTT GAGCTTGACC AAGGAGCCTG CTGATTGTCT CAATGGGATA GAAACCTTGC TGTGCTCTTG GCTAGAGGAG ACCTCGGACA CAGGCCGACA CATCCCACAC AAGCAGAAGG AGAACGCTGC TGCTGCCCTG GTGGCTTTGG CGTGTGCCAG GGGGTCACTG AAGACCTTTG TCCACACAGT TCACCTGCTG CAGAGACAGA CTGAGCTGGG CTCCCTGCCT GTCGCCGATG TTTTATATAG GTTGCTGCTT TTGGAAGGGG GACCTGGATC CCCATCCTGT TTGCTTGGGG GCAAACACAT AGTATCTTGG GGATATGAGG ACATGCTGCC AGCACCAGAC AGCAACACTG GCTCCAGCTC TGAAAACAAA GATGCTGACT TGGGCCGCTG TCTCACCGCA GATGGGCATT ACCTGTACAC TACCAACTCA GCCGGAAGAG GAGTGAGCAA GCTGGGCTCT GGACTGCATG GCACGCTCAG AGGGTTTGTG TACTGTCGCA ATGAGGAGCT AGAGCCAGGG TGGGTGGCGT ACGGCAATGG CCACCTACTC CATCGGCCTG TGTCTTTTGA CAATAAACCT CACTCCCTTT TCCAGGTCAT AGACCAGAAC ACCCTGCAGG TGTGCCAGAC AGTGCCCATG CCCGCCAGCC CCCTTCCTGT GGGCAGCACC ATGAGCACTG TGCACCTTTC CTCAGATGGC ACTTACTTCT ACTGGATCTG GTCCCCTGCC AGTTTAAATG AGAAAACCCC AAAGGGACAC TCTGTCTTCA TGGACATTTT TGAACTTGTG GTGGAAAATG GGGTATTTGT AGCCAACCCG CTTCAGGAAC GCACAATCCT AATGAGAAAA GAGGGTGAGT CTGCTAAAAG CATTAATGAG ATGCTCCTGA GCAGACTGTC TCGGTACAGA GCCTCTCCAT CGGCGACTCT CGCGGCCCTG ACTGGCTCCA CCATCTCCAA CACGCTGAAG GAGGATCAAG CAGCAAATAC TAGCTGTGGC CTTCCTCTGA AAATGCTGCG GAAGACGCCC ATGTACACCT GTGGCACGTA CTTGGTGATG CTGGTCCCAC CTCCAGGAGG GTCAGGCAGC TCTGCCACCC GCTCTCTCTT TGGTGGAACA AGTGGTCTGT CGTCTTTAAA GATCCTGGCC TCCAGTTTGG TGTATAACAT CTCAGATGGT CAGTTCGCAA GTCGTGCAGA TCTCATAGAT GCTGCTGGAA GCTCGCTGGG GCGTGGTGCT CTTGTACCAG GATTGGGTGC CTGTTATGAT ACCATGAACA ACATGCTATG GACTTGCTCA AATGACTACA TCGACCAGTG GTGCAATCCT GGGAACCAGG CCTTCCATTA CGTGTGCCAG AGACTGGGTG TCAGCCATAT CATCACCGAG CCCAAAGAAG AGGCCATTAG CACAAATGAA GTCATAAACC AGTTACTGCA CCATGTTGGC GCCATGTGCA TTCACCAGCT CAACCTTCTA GCTACGAACC CCAACCTTCC CATCACTAGT GTCCTGGGCA AGCAGCACCC AATTGAGGCA CGACATCTGA GCAGCATCTG TGACATCATG GAGAAGGCCA TGGTTAATGG CGACACCTGC ATCGTACGCT GCATCCTCGT CGTCTTTCAG GTGGTGTTTA AATTTTTCTT CAGCCCACAA ACTGAAAGGA ACCGAGACAT CATTCGGCGG TCAGGATTGC TTCTTTGGCA GTTGTTGATG GCACCAAAAG ATCAAATCTG TCCTGAAATT CAGAAGGAGG TCTGTCTTGC CATCAGCTCT GGTTTAAATA TTTTATATCC CGGTGAAACA GAAATTAATA ACTTACTTAA GCTGGTCTTA ACAGAAGGGG AGAGAAACAG TGGACTCTCC CAGCTCCGTG ATGTGATCCT CACCAACCTG GCCGAGCAGC TTCAGAACAA CCGATTTGGC AGTGATGAGG ACGATCACTA CAGACTCAAT GATGAGCTCT TACACTACAT CCTGAAGATT GTCGTGCGGG AATCCTGTAT CCTAATCACC AAGTGCCAAA CGGTCTCCAA GGACGACTTC CAGAAGCTCC TCTCGACGGT GCCTGCTGCA TCCTCCTGCC TGCGCTATCT GATGGCGGTT CAGAACCACC TCCTCAGTAA CACTGTTTTG ATTAAGCCTG ATGAGAACGA GGACAGTGAC AGCTCCTTGC AGGGAGAGAC ATTGAAGGAG TTAAAGACCA GTATCTTGGC TCTTGCCACC CAGATCCTGA CTGGCTGTGA TGAGGTCCTG GAAATGCTGC AGCAGGTCAC CACAGCCCTC ATAAACAGTG ACATACCTGA CCGAGAGCAG AGGTTAAAAG GCTTGGAGCA AGTTACCAAG GCCACTATGC TTGGTCACCT TCTCCCAGTG TTGCTGACCT CCCTGATGCA CCCCAATCTA CAGACCCTGA CCATGGCCGA CGCCCTGATG CCTCAGCTTG TGCAGCTGGT GCTGTACACC AGCCAGACGG CCTTGCTGCT TAAAACCCAG TGTCCAGCGT TTGCCGAGAT GAGCTGTTCC CCATGTGGCA CCTCAGACCA GAAGTGCAGG CTTTTTCCTG ATGAGAGAAT GCTAGAAGAA AAGGAAGAGC CAGGCTTCCT CACTGGCTTA AAGATCCCCG CCCCATGGGC TGCTGGGAAG ACCGTGGAAA CAGTACACCC TGTCAGAGAC AACTATAAGT TTAAGGAGAC TGTTCATATC CCAGGAGCTC GCTGCCTGTA CCTACGATTT GACACCAGAT GTTCGTCACA GTACGACTAT GACAAATTGG TGATTTACGC GGGGCCTAAC ACAAACAGTA GGAAGGTTGC TGAGTATGGA GGCAACACTC TGGGATATGG CAGCCGGAGT GTCTTAGGAA CTGGCTGGCC GAAGGACTTG GTGAAGGTTG AAGGCGATAC AGTCACCTTC TCCTTTGAAA TGAGAAGTGG CCGTGAACAC AACACTCCTG ACAAAGCCAT GTGGGGTTTT GCTTGCACAG TCCGCGCTCA GGAGTCCTCG GAGGATGTCT CGGGAGGCTT GCCCTTCCTG GTAGACCTGG CCTTAGGGCT GTCTGTGTTA GCTTGTTCCA TGTTAAGAAT CCTATACAAC GGACCAGAAA TTACCAAAGA AGAGGAAGCT TGTCAGGACC TGTTGCGATC CAAACTTCTA CAAAGGTGCC AGTGGCAGGT GGAGGCCAAC GGTGTTATCT CCCCTGCCCT TACTCCGAGT CCCTCTCCAC TGCCGCTGAC CATAGAGGAA GACAGAGAAT TCACCTACCC CGCGGATGTC CTTGTGCCTC CTGTTGGGAA CTACTTCGAC CTGCCTCGGA TCAGGCTGCC TCCAGGAATC ATGATAAAGC TCAGGGAGAT TTCTGGCCGT GCACGACCTC AGTTTAGACC AAGTATAAAA GAAGTGATCC AGCCCGATGT CATGGAAGAA ATGGTGGTGT CCTGTGTTAT TAAGCACTTG AACTTGGTTG ACGCGCTGCA GTCCCTGATC AATTTCCAGT ATCAGGAAGA ACATGCTGAG GAATATGACT TATTGTGTAA AATTATGGGA GAGACCTTTA AGAAGCTCAA TGCCATGGAG AGACAGCTGC AGAGTGTGGC TGAGCTGGAA CAGAAGTGGC AGAGTGAGGC TGAGGACGCC ATGCAGGGGA AGCTGGAGAA TAACATGCCT TTCTTTTATG ACTACCATTT TAATGAGAAC AAAATGAAAG AACTAGAGCT TTTGTGTTCC ATGAAGGAAG TCTCCTTTGA TGGGAATGAT CTTGAGAACA TGGTCCTCTC ACTGAGGGAG AAGTTCCTCC AGGAAGTGAA TTCTCTTGCT CAGAAGCCCT CCCACCCACT GGCCAAAACA AAGACCCTGG TGAGGAGCCT GATGAACCGA GCAGAGCTGC TGCTGCACGT CACCATTGCG GCCCAGGCTG GGCTCACCAG GAGCATCTCA GGGACGCCAG CAGAGACGCC TGCATGCAAA TCAGCTTCAG AAACAAAGGT GGCGTCTCAC GCTGTCCGGC AGCCTGTTTT CCTTCGAAGC ATGTCTGCTC CTTCTGACCT GGAAATGATT GGTAATGAAG ACTTGGAATT TACTAGAGCA AATCAAAGGC GTCGGCACGT GACCAGCCAC CGCAGCAGCT CCTTTACACT TCTGCAGTCA CTGGCCATCG AAGACAGCAG GGACAAGCCC ACTTACAGTG TCCTGCTTGG GCAGCTGTTT GCTTTCATTG GCACCAACCC TGACCAAGCG GTGTCCAGTA GCAGCTTCCT TTTGGCTGCA CAGACAAGAT GGAGGCGGGG GAACACTCGG AAGCAGGCCC TGGTGCATAT GCGGGAGTTG CTGACTGCTG CTGTGCGAGT TGGGGGAGTC ACACACCTGG TAGGGCCAGT GACAATGGTC CTTCAGGGGG GACCCAGAAT TGAAGAGCTC ACCTGTGGTG GGATGGTGGA GCAGGTCCAG GAAGCCTTTG GTGAGACCAT GACCTCTGTC GTGTCTTTGT GTGCTCGGTA CCCCATTGCT TGTGCGAACA GCATTGGACT CCTGTGTACC ATACCTTATA CCAGGAGTGA AGAGAAGTGC CTGGTCCGAA GTGGCCTCGT GCAGCTCATG GACCGACTCT GCAGCCTGAG CAGCCAGACC GAGTCCAGCT CCAGTGAGAA GCAGACAAAG AAGCAGAAGG TGGCCACCAT GGCCTGGGCT GCCTTCCAGG TGTTGGCTAA CCGCTGTGTG GAGTGGGAGA AAGAGGAAGG TGGCTCCACA GAGGCTGTGC ACTCAGGGCT GGCCCGGCAG GTGTCCAGTC TCCTCACCAA CCACCTTGCC CGAGCCACTG AGTGCTGTGG CAACCAGGCT GCTGGGAACG ACGCCCTGCA GGATGTCCTG AGCCTTCTCA ATGACCTCTC CAGGAGCCAC ATAGGGAAAG CCATCTTGAG CCAGCCAGCC TGTGTTTCTA AGCTCCTCTC ACTACTGCTA GATCAGCGTC CATCCCCAAA GCTGGTATTG ATCATTCTTC AATTGTGCCG GGCTGCTTTG CCTTTGATGA GCGTGGAGGA CTGTGGGAAT GTAGAGCTCC CGCCCTGGAG CTACTCCGTC CCCTCCCTGA ACAGCGAGCA AGAGGACCCC AGTGACCCTG CCTCCAAGAT TGCCTCTCTG CTCTTAGCCA AGCTGGCAGA CTATGTGGTC CCAGGATGTC AGACGGTTCT CTCTCCAACT GCTTCTGAAC CTGACACCAC ACTGACAAAA ACCAGTCCTA AGAATTCCTT AAAAGGAGAT AAAGATCCTG GAGAAGAGAG TGAAGCAGTG GATGGCAAGC TCTCCATATT TATCCACAAG CGGGAAGACC AGTCCTCCCA TGAGGTCCTC CAGCCACTGC TGAGTAGCTC AGAAGGACGG CCCTTTCGAC TCGGCACTGG TGCCAACATG GAAAAAGTTG TGAAGATGGA TCGAGACATG ACCAAGGGTG GCTGCTGTGA AGTGATCACA GAAGAGGCCT CAGCCGCCCT CAGGAAAGCA ACCAAGTGGG CACAGTCGGG CCTCATTGTC AGTGTCGGAC CACCAGTGGA ATCCATCAAC CCAGAGACTG TGAGTGGGCT GTCCACAGGT GACAAGAAGA AAACTGCCCA AACCTCCATC TGCCGAGAAA GAAACTCTGA ACTGGCCAGA ACTGACCCCG TGCGACCCTT CATCAGTGGA CATGTGGCAA ACAGCATGGC TGCAGAAGTG ATCGCCTTGC TTCATAGCCT GCTAATGGCG CCAGAATCTA ATGCTGCTCA AATATGGACC ACAACGGCAG AAAAGGTTCT GTCTCGAGCA TTGATGTACA TTCCACAACT AGGGAAGTAT GCAGAAAGCA TCCTGGAGAA TGGGAGCAGC AGTGGCAGGA AACTGGCCAA GCTTCAGAGG ATTGCACGCC AGGCAGTTGC CGCCCTCTGT GCACTCGGAG GCTTTAAAGA GACAATCAAG ATAGGCTCCG AGGTCCAGGT TTTAGGTAGA GGAATATCAG GAAGCATTGG AGTTGTGGCT TCTATCAATG AACAGGAAGG TATAGCTACA GTCCGGTTCC CTCCTGTGGA CTGCCGAAGG ACGTCACAAG CAGCAGACAC GCTGACTATT CCATTGTCTA GACTTTGTGT TCCAAGGTCA GAGGCACTGC CTCTTCATAA GCTGTCCATT ACTGAGAAGG TAGTCCAGGC AGTGCAGTCC ATGCTGCTCC CTCAGGAAGG AAGCCTCTCT ATCCACACCT CTCTTCCTGC AACAGGAGAT GGCTCAGCAC CTGTCATGGC AGTTGTCCGG CTTCTGGCTG AAATCAGAAC CAGAGCATGC CTGGTCATGG CACAGCTTTT AGAAGACAGC CTGTTTTGTG AAGAGTTCAT ACAGCAGTGT CCAGCAGCTG TGGAAGTTCT GAACCTCGTA GCCCAGGAGT GCAGTGCGGG AGAGCGGCTG GCAGTTGTAG AGATGCAGTG TGAGAGGCTC AGGATGCTCT ACCGGGACTG CGCTCGGCCC CCTCCACCTC CGCTGCAGGC TGACCGGAGA CAGCCCAAAG AGATCACCTG GAGCCCTTCT CGAGTGTTCC CTCCAGTTCG TGCCTGCATG TTCTCATCTC ACCTCACCTC TGTGACCTTC CTGGCTGACC CCAGCGCTGG AGGAGGTCTT CCCCGGGGCA CATTCATCTA TGCCACCTCG CCCTTGCCTG TTCAGGCCCC ATCTTTCTAC TGGGAAATTG AGATTGTTTC CTATGGAGAC ACTGATGATG ACACTGGACC AATCGTCTCA TTTGGCTTCG CTACAGAAGC TGAGAAGCGA GATGGGGCAT GGACCAATCC AGTGGGCACT TGCCTCTTCC ACAACAATGG CCGAGCTGTA CACTACAATG GCTCCAGTCT ACTGCAGTGG AAGAGCGTCC GCTTAGATGT CACACTCTCT CCTGGCGATG TGGCAGGAAT CGGCTGGGAG AGGACCGAGG GGACTCCACC TCCTCCAGGG CAGCCAGCCA AAGGTCGGGT GTACTTCACA TACTGCGGGC AGCGCCTCAC ACCATACCTA GAAGATGTCT CTGGTGGCAT GTGGCCTGTG GTTCACATTC AGAAAAAGAA CACAAAAACT CGAGCCAATT TTGGCTCCCG CCCATTTGCG TATGCTGAAG GACAGGCCCA CCGAAATGCT GCCGACCTGT GCACTGACCT GGCAGAAGAG ATCAGCGCTA ACTTTGAGGC CTTGCCATTC GCCATGGCAT CTGACAGTGA CAACGATGCT GGCACCAGTA TTGCATCAGA CCCAGGCACT CATGGGCCGC CATGCCGGAT TGCAGCAGTG GCCACAGCCC AGCAGCAATA TGACAGTGAC ACCTCCTGCC ACTATAAAGT AGAGCTCAGC TATGAGAATT TCATTACCTC AGGCCCAGAC CCCCACCCAC CTCCCATTGC GGATGATGAG AGTGATGATG ACGACGATGA TGACATCCCT CAGGAGGACC ACTACGCCCT GCTGGTGAAA GCATGGGAGA CCAAGGTTTT CCCTACCATC CGGAGACGCT TCCGCAATGA AGCCGAGCGA AAGTCGGGCC TAGACCAGAT CAAGGGTGCT TTACAACTAG GCATGGTGGA CATCGCCCGG CAGACAGTTG AGTTCCTCTA TGAGGAGAAT GGCGGCATCC CCAGAGACCT TTATCTTCCT ACCATTGAGG ACATTAAAGA TGAAGCAAAC AAGTTCACAA TCGACAAAGT TCGAAAAGGT CTCACGGTTG TCACCCGCTC TCCAGACAGC AGCAATGTAG CTAGTAGTAC CGTGGGAACT GCTCTGCCAA AATTTGCCAT CCGAGGGATG CTAAAAACCT TCAGCCTTCA TGGAGTCGTC CTAGATGTTG ATTCAGTGAA TGAATTGGTG CAGGTGGAGA CATACCTGCG GAGTGAAGGG GTGTTGGTGC GGTACTGGTA CCCCATTGAT ATGCTAGAAA GGCCCCCTGC AGGCTACCGG AGGACTGCCA CCAATGGCCT GGTCACACTG GACAATACCA ACCTTCAAAT TCACAGAGAG CTGCTGCGAT GTGAGGCTGC ACTGGCCCGA CTGTACTGTC GCATGGCCCT GCTTAACATC TTTGCCCCCA AGCTACCTCA TCTATTCACT CGGCTCTTCC ACATCCCTGC CATCCGAGAC ATCACGTTAG AGCACCTGCA GCTCCTGTCC AATCAGCTCC TTGCTCCTCC CCTCCCAGAT GGCACCATCA GCTCCAGCTC CATCCTCCTG GCACAGTCTC TGCAGCACTG CATCCACTCC CAGAGCTGCT CTGCCACCGA CCTCTTCTAC CAGGGCAGCT CGCAGACACT GAGGGAGTGG CTCAGCGTGG CCATCACCCG CACCCTGCAC CAAGGCGAGG ACAGCCTCCT GGAGCTGACA AAGCAGATCT GCGCCTTCCT GCAGACAGCA CCAGAGCAGT TCCCCTCCGA GGAATTCCCA GTGTCTGAGT CCAAAGTCAG TATGGATGTG AATTTCCCTG GAGCAGCTTT TGTTGTTGTG TCTTGCAAAG AAAGTCAGTC TGGATTCCGC AAAGACTCCT CTTTGTACAA GGCTCCGTGG GCACGCGTGC TCGTCTATGG GCTTGGCCAC AAAGTGAAGC GTACCGGCCA GCTGAACCTC ATTGAGGCTG CCTGCTACCC TCGAGACGCC TCCCCAGCCA ACACTGGGCT CGCCCCTCCA CCCACAGCTG ACCAGTACCC TTCTGTGGTT CTTTCCACAG ACAAAGTCCA CATCAAACTA GGAGTGTCCC CACCACCTGG AGCAGTGCTG GTCTTGCATT CTCTGCCCCT GGAGTTCCCG CTGGCGATGG CCTTTGCCGA GCAGCTGCTG TCCTGGAAAT CAGAGGACAG TGATGGGAAG TCAGAGGATG AGCCTGACAG CATCCCTGCC TCCGTCCTCC TGCACGTGGT GGAACTGCTG GGAAACTTCC TGTGGACTAC AGACATGGCG GCCTGTGTGA AGGAGTTGGT CTTCCACCTC TTGGCTGAGC TCCTACGCAC AGTACACACC CTGGAGCAGC GGAAGCACCC AGCAGGCCTG TCCTCCTCCA TCGCCCTCCA GCTCAACCCC TGCCTGGCCA TGCTGATGGC CTTACAGTCA GAGCTGCATA AGCTGTATGA CGAGGAGACG CAGAGTTGGG TCTCGAGCAG CGCCTGTGGG GGCTCTGGAG CAGCAGCTGT GGGCGACCAG GGCAGGTTTT CCACCTACTT CCACGCCCTC ATGGAAGGCT GCCTGGCAGT TGCGGAAGTA ACCCTACCTA CCAATATCAG TGTCACAGCT AGCGGGGTGA CCTCAACGAC CACCCCAAAT CTCAGCGACT CGTCCTCTTC CTCCTCCTCC TCCCCAGGAC AGACACCACA GAGCCCTAGC CTACTGTCCA AGAGAAAGAA AGTCAAGATG AAGCGGGAGA AGGCCTCCTC CTCCAACAAG CGACAGTCCT CCCGTGCCTC AGACGCAGAC TCCACAGTGC TCAGCATTGG GGGAAGCAAG CCTGAGGACA TGCTGTGGTT CCACCGTGCC CTCACCTTGC TTATCATCCT TCGGCACCTC ACCAAAAAGG ACCCACAGGG CCTGGGTGTA ACGAGCGATG CCATTGCTGA TGCCTGCCAG GCCCTGGTGG GCCCCACTGC CCATAGTCGC CTGCTTGTGA TCTCTGGCAT TCCTACCCAC CTAGATGAGG CTGTTGTCAG AGGGGCCATC CGCAAGGCCT GCAATGCCCA TGGCGGTGTC TTCAAAGACG AGATCTACAT TCCACTGCAG GACGAGGACC CTAAGAAGCC AAAAGACAAG GCTGAGGGCA GCGACAGCAA GGTGGAATCT GAGAAGCTGC TGGGGTTCCC CAGTGTGGAC AGCCTGGAGG GGAGCACGTC GAGCAGCCTG GCCCCCGCCA TGAGCATCAG CGCCTCGGCC TCCACCAGCC AGGCCTCGGT CTGCAGTTCA CAGTGTGTCT CACAGACTGC CAGTGACCTC TCAGTGGACC CACTGCCCTC AGGCCTGGAG CTTCCTGTCC CTCCTGTTCT GTTGGAACCC CATGTAGTGT CCAGCCAGGA GAGCCTGGAC ATTTCGTTAT GCAGCACCGG CAGCCTGGGC AGCCTGGGCA GCCTGGGGGA GCCTCTAGAC AATCCAGAGA CGGCATCTGT GTCCGACGTG GGCTCAATGT ACACGGTCAC ATCCCTGGAT ACCCAGCCCC TCGCAACACG CCCTATCAAA GGCTTTGCAG TGGTGGAGAT CAGGTCCCGA GCCAAAATTG AGAAAATCAG AGCAAGTTTA TTTAACAATA ATGATTTGAT CGGTCTGTCA AGTCTGGATG GTGAAGACGA GCTGATGGAA ATGTCGACAG AGGAGATTCT GACAGTGTCA GTGGTGAACC AGAGTCTGTT TGACACCCAA GGGAGTCCAG GGCTGGAAGA TTACTTCAGT GACAAGTCCA TTAAAGGTGA GAAACTGGTG CCTGGGGCCA GAGAGGTCCT GACAGAAATA TTTAAAAGCT GTGCCCACTC AGAGCAGACG CTGAGCCTGA CACCAGCCAA ACCCATCAGG GTCTCTGACA TCTATCTTAG CAAAGAGCAG ATCAACTCCC AGACTCCAGG CAACCTCCTA CACCTCTTCT TCACCAATGT CCGGCCTCCA AAGAAAGTGC TGGAGGACCA GCTCACACAG ATCCTGAGGA AATACGGCGT GCCCAAGCCC AAACTGGATA AGAGCAAGTA CAGCAAGGCT GGCAAGGAGC AGCACCCGGT GAAGGTGGTG AGCACCAAGC GGCCCGTCAC CAAGCCGCCT GCCAAGGACA AAGCCGTGCT CAGCAGCCTC AGCAGGACTG CACTAAGTGA GAAGAAGCCG ACTGTGAAGC CAAAATCACC TGAGAAGAGC AAACCAGACG AAAAGGATCC AGAGAAGTCA CCCACCAAAA AGCAAGAAGT TCCTGAGGAA AAGTACCTAA CTCTGGAAGG ATTTCACAAA TTTGTCATTG ACCGAGCCAA GCAAGACATC CGCAGTGTCT GGAGGGCAAT CCTATCCTGT GGTTATGATC TTCACTTTGA GAGGTGTGCC TGCATTGATG TTCGACATGC ACAGAAGGCC TCCAGGAAGT GGACCCTGGA GATGGATGTG GCTCTCGTGC AATACATCAA CCGGCTGTGT CGCCATCTTG CTATCACACC AGCACGGCTC CATCCTCATG AGGTGTACCT TGACCCAGCG GATGCTGCTG ACCCCAGGAT GGCCTGCCTC TCAAATGTGC CTATTGAGAG CTTACGCCTG CGCTTCGCAC TGCTCCAGTC TCTCAACACC ACACTGGAGA CCTTCTTCTT GCCCCTGGTA GAGCTGCGTC AGACACCCAT GTATGCACAC AGCATCGCGG CCCTGCTGAA GGAGGCCAAA GGGCTGATCT TCTATGACAC AAAGGTGACT GTGATGAATC GGGTGCTAAA TGCAACTGTA CAGAGGACAG CCGACCATGC AGCACCTGAG ATCACCTTAG ACCCACTGGA AATTGTGGGA GGGGAGATCC GAGCTTCTGA AAACTCCTAC TTCTGCCAGG CTGCCCGGCA GCTGGCCTCC GTGCCATCTT CTCAGCTCTG TGTGAAGTTG GCCAGTGGCG GCGACCCCAC ATATGCCTTC AATATCCGCT TCACCGGGGA GGAGGTTCAT GGCACCAGTG GCTCCTTCCG TCACTTCCTG TGGCAGGTGT GTAAAGAGCT GCAGAGTTCC TCACTGTCGC TGCTCCTGCT GTGTCCCAGC TCTGCAGTCA ACAAGAACAA GGGCAAGTAC ATCCTGACAC CAAGCCCCAT CACCTATGGG GAGGAGCAGC TGCTACACTT CTTAGGCCAG CTGCTGGGGA TCGCCATTCG TGCAGATGTG CCTCTCCCCC TGGACCTGCT GCCCTCCTTC TGGAAGACGC TGGTTGGCGA GCCTCTCGAC CCTGACCAGG ACCTGCAGGA AGCAGACATC CTCACCTACA ATTATGTCAA GAAGTTTGAG AGTATCAATG ATGAGAGCGA GCTGGAGGCC TTGTGTGCCG AGATTGCCTC CCAGCACCTG GCCACCGAGA GCCCTGAAGG TCCCAAGCCC TGCTGCCGGT TCACCTACCT GACCATGACT GGCGAGGAGG TGGAGCTATG CAGCCGTGGC CGGCACATCC CTGTGGCTTG GGAGAACAAG GATATCTACG CCGCAGCCAT CCGGAGCCTG CGGCTGCGTG AGCTACAGAA CATGGAGTGC GTGACAGCCG TGCGTGCTGG CCTGGGCTCC ATCATCCCCC TGCAGTTGCT CACCACCCTC AGCCCACTGG AGATGGAATT GCGTACCTGT GGCCTCCCAT ACATCAACCT GGAGTTCCTG AAGGCCCACA CCATGTACCA GGTGGGGCTG ATGGAGACAG ACCAGCACAT CGAGCTTTTC TGGGGGGCAC TGGAGATGTT CACCCAGGAG GAACTGTGCA AGTTCATCAA GTTCGCCTGC AACCAGGAGC GCATCCCCTT CACCTGCCCT TGTAAGGACG GGGGCCCTGA CACAGCCCAT GTGCCACCGT ACCCCATGAA GATTGCCCCT CCAGATGGCA CAGCAGGTCC CCCCGACTCA CGCTACATCC GAGTGGAGAC CTGCATGTTC ATGATCAAGC TCCCCCAGTA CTCCTCCCTG GAGACCATGC TGGAGAAGCT GCGCTGTGCC ATTCACTACC GCGAAGACCC CCTCAGTGGC GGATCCGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGTAGCGGCC GCGAAGGATC TGCGATCGCT CCGGTGCCCG TCAGTGGGCA GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA TTGAACGGGT GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG GCTCCGCCTT TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA CGTTCTTTTT CGCAACGGGT TTGCCGCCAG AACACAGCTG AAGCTTCGAG GGGCTCGCAT CTCTCCTTCA CGCGCCCGCC GCCCTACCTG AGGCCGCCAT CCACGCCGGT TGAGTCGCGT TCTGCCGCCT CCCGCCTGTG GTGCCTCCTG AACTGCGTCC GCCGTCTAGG TAAGTTTAAA GCTCAGGTCG AGACCGGGCC TTTGTCCGGC GCTCCCTTGG AGCCTACCTA GACTCAGCCG GCTCTCCACG CTTTGCCTGA CCCTGCTTGC TCAACTCTAC GTCTTTGTTT CGTTTTCTGT TCTGCGCCGT TACAGATCCA AGCTGTGACC GGCGCCTACG CTAGACGCCA CCATGGAGAG CGACGAGAGC GGCCTGCCCG CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG GCGGCGGAGA GGGCACCCCC AAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GGTGGGCACC GGCTTCCCCG AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC ACCCCATGGG CGATAACGTG CTGGTGGGCA GCTTCGCCCG CACCTTCAGC CTGCGCGACG GCGGCTACTA CAGCTTCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG CTGCACAGCA ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCCATC GCCTTCGCCA GATCCCGCGC TCAGTCGTCC AATTCTGCCG TGGACGGCAC CGCCGGACCC GGCTCCACCG GATCTCGCGA GGGCAGAGGA AGTCTTCTAA CATGCGGTGA CGTGGAGGAG AATCCCGGCC CTATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GAAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TCCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGCC GCCTCCCCGC CTGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA AATAAGATCT GCTTTTTGCT TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTA GTAGTTCATG TCATCTTATT ATTCAGTATT TATAACTTGC AAAGAAATGA ATATCAGAGA GTGAGAGGAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG CTCTAGCTAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA
