NM_178029.3;KMT2F; mKIAA0339; mNSC1; Nsccn1
100μg liquid plasmid(Instantly):$149
| name | pLV3-CMV-Setd1a(mouse-repeat-opt)-3×FLAG-CopGFP-Puro | another name | apLV3-CMV-Setd1a(mouse-repeat-opt)-3×FLAG-CopGFP-Puro |
| Insert Size (bp) | 5151bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGG ACCAGGAAGG TGGGGGAGAC GGGCAGAAAG CCCCGAGCTT CCAGTGGCGG AACTACAAGC TCATAGTGGA TCCTGCTTTG GACCCTGCTT TGCGCAGGCC TTCCCAGAAG GTGTACCGCT ATGACGGAGT CCACTTCAGT GTCAGTGACT CAAAGTATAC CCCCGTGGAA GACCTCCAGG ACCCCCGCTG TCATGTCAGG TCCAAAGCCA GAGACTTTTC CCTCCCGGTT CCTAAGTTTA AGCTGGATGA GTTCTATATT GGACAGATAC CACTGAAGGA AGTAACTTTT GCGAGGCTCA ATGACAATGT GAGGGAAACC TTCCTGAAGG ATATGTGCCG GAAATATGGA GAGGTGGAAG AAGTGGAGAT CCTTCTTCAT CCCCGCACTC GAAAGCACCT AGGCCTGGCC CGTGTGCTCT TCACCAGCAC TCGGGGCGCC AAGGAAACTG TCAAAAACCT CCACCTCACC TCTGTCATGG GCAACATCAT TCATGCCCAG CTTGATATCA AAGGCCAACA ACGAATGAAG TACTATGAAT TGATTGTCAA TGGCTCTTAC ACTCCTCAGA CTGTGCCCAC TGGGGGCAAG GCCTTGAGTG AGAAGTTCCA GGGTTCTGGT GCAGCCGCTG AGACGACTGA AGCCCGCCGA CGGTCTTCCT CGGACACAGC TGCCTATCCA GCAGGCACTA CTGTGGGGGG CACCCCTGGC AATGGCACTC CCTGCTCCCA AGATACAAAT TTCTCCAGCA GCCGACAAGA TACTCCATCT TCCTTTGGCC AGTTCACCCC TCAGTCCTCC CAAGGAACCC CCTACACATC TCGGGGTAGC ACCCCCTACT CTCAGGACTC TGCCTACTCC AGCAGCACCA CTTCAACCTC CTTCAAACCC CGGCGGTCAG AGAACAGCTA TCAAGATTCT TTTTCTCGCC GCCATTTCTC TACATCTTCA GCCCCTGCAA CCACCGCTAC TGCCACCTCA GCAACTGCTG CAGCTACTGC AGCCTCCTCC TCATCTTCCT CCTCCTCGTC ATCGTCCTCC TCCTCGTCGT CTTCTTCCTC AGCCTCTCAG TTTCGTGGTT CTGACTCTAG CTACCCAGCA TATTATGAAA GCTGGAATCG TTACCAGCGC CATACTTCCT ATCCACCTCG CCGGGCAACT AGAGAGGACC CTTCTGGGGC TTCATTTGCC GAAAATACGG CTGAGCGCTT CCCACCTTCC TATACCTCCT ATTTGGCCCC TGAGCCTAAC CGATCCACTG ACCAAGACTA CCGGCCTCCT GCCTCAGAGG CCCCACCTCC AGAACCTCCA GAACCAGGAG GTGGTGGCGG AGGATCTGGA GGGGGTGGCG GGGGAGGAGG CGGAGGTGGT GGGGGAGCTC CAAGTCCTGA GAGAGAAGAA GCCCGGACTC CCCCACGCCC AGCTTCCCCT GCACGCTCTG GCTCCCCAGC CCCAGAGACC ACCAACGAGA GTGTGCCCTT TGCTCAGCAC AGCAGTTTGG ATTCCCGTAT TGAAATGCTG CTGAAGGAAC AACGCTCCAA GTTTTCTTTC TTGGCTTCTG ATACAGAGGA AGAGGAAGAG AACAGCAGTG CAGGCCCTGG GGCTAGAGAT GCAGGGGCTG AGGTGCCTTC TGGGGCAGGT CATGGGCCCT GTACACCTCC TCCTGCACCA GCTAATTTTG AAGATGTAGC ACCTACAGGG AGTGGAGAGC CAGGGGCTGC CCGGGAGTCA CCAAAGGCCA ATGGACAGAA CCAGGCTTCT CCATGTTCTT CCGGAGAAGA TATGGAGATC TCTGATGATG ACAGGGGTGG CTCACCACCT CCAGCCCCAA CACCCCCCCA GCAGCCGCCA CCGCCACCAC CTCCCCCTCC TCCCCCCCCT CCTCCCTACC TTGCTTCCCT TCCCCTTGGC TATCCTCCCC ACCAACCTGC CTACCTCCTC CCACCCCGCC CAGATGGACC ACCCCCCCCT GAGTACCCTC CGCCACCTCC TCCACCACCA CCCCACATCT ATGACTTTGT GAACTCCCTG GAACTTATGG ATCGACTTGG GGCACAGTGG GGAGGGATGC CCATGTCCTT CCAGATGCAG ACCCAGATGT TAACACGGCT CCACCAGTTA CGGCAGGGCA AGGGATTGAC TGCAGCCTCA GCTGGTCCTC CTGGCGGGGC TTTTGGCGAG GCCTTTCTCC CTTTTCCACC TCCACAAGAG GCAGCCTATG GCTTGCCCTA TGCTCTGTAC ACACAAGGGC AAGAAGGTCG TGGGTCGTAC TCCCGGGAGG CCTACCACCT GCCCTTGCCT ATGGCAGCCG AGCCCCTGCC CTCTTCCTCA GTCTCGGGAG AGGAGGCGCG GCTGCCTCAT AGGGAGGAAG CAGAGATAGC AGAAAGCAAG GTCCTACCAT CAGCAGGCAC AGTGGGTCGT GTGCTGGCTA CCCTTGTCCA AGAGATGAAG AGCATTATGC AGCGAGACCT TAATCGAAAG ATGGTCGAGA ATGTGGCCTT TGGAGCCTTT GACCAGTGGT GGGAGAGCAA GGAAGAGAAG GCCAAGCCAT TCCAGAATGC AGCCAAACAG CAAGCCAAGG AAGAGGACAA AGAAAAGATG AAGCTCAAAG AGCCAGGCAT GCTGTCCCTC GTAGACTGGG CCAAGAGTGG CGGTATCACA GGCATTGAGG CCTTTGCCTT TGGGTCAGGG CTTCGAGGGG CCCTGCGGTT GCCTTCATTC AAGGTAAAAC GGAAAGAGCC ATCAGAAATT TCAGAGGCAA GTGAGGAAAA GAGGCCCAGG CCCTCTACTC CAGCTGAGGA AGATGAAGAT GATCCTGAGC GAGAGAAGGA AGCTGGAGAA CCAGGACGTC CAGGGACCAA GCCTCCGAAG CGAGATGAAG AACGGGGCAA GACCCAAGGC AAACACCGAA AATCCTTTAC TCTGGACAGT GAAGGGGAGG AGGCGTCCCA GGAGTCTTCC TCAGAGAAGG ATGAGGATGA TGATGATGAA GATGAGGAAG ATGAAGAACA GGAGGAAGCT GTGGATGCCA CCAAGAAGGA AGCAGAGGCA TCGGATGGTG AGGATGAGGA CAGTGACTCG TCCTCCCAGT GCTCTCTGTA TGCGGATTCA GATGGTGAAA ATGGCAGCAC ATCAGACTCC GAGAGTGGCA GCTCGTCTTC CTCTTCCTCA TCGTCTTCCT CCTCTTCCTC CTCTTCCTCA TCAGAGTCCT CCTCTGAGGA AGAGGAGCAG TCAGCAGTCA TTCCCTCAGC TTCACCCCCC AGAGAAGTTC CAGAGCCCCT TCCAGCTCCT GATGAGAAGC CTGAGACAGA CGGGCTTGTA GATTCCCCTG TCATGCCTCT CTCTGAAAAG GAGACATTGC CCACACAGCC TGCAGGCCCT GCTGAAGAAC CACCTCCTAG TGTACCACAG CCTCCAGCAG AGCCACCCGC CGGACCGCCA GACGCTGCCC CCCGCCTGGA TGAGCGCCCC TCTTCTCCTA TCCCTCTCCT ACCCCCACCC AAGAAACGCC GGAAAACTGT TTCCTTCTCT GCTGCAGAGG AGGCACCAGT CCCAGAACCT TCCACAGCTG CCCCACTGCA GGCCAAGTCT TCTGGTCCAG TCTCTCGAAA AGTCCCCCGG GTCGTAGAGC GGACCATTCG TAACCTCCCC CTGGACCATG CGTCTCTGGT CAAGAGTTGG CCTGAGGAGG TCGCCCGAGG GGGTCGGAAT CGAGCTGGAG GTCGTGTGCG CTCTACTGAA GAAGAGGAGG CCACTGAGTC AGGGACAGAA GTAGACCTGG CAGTACTGGC TGACCTGGCC CTGACCCCAG CCCGACGTGG GTTGGCTACC CTACCCACTG GTGACGACTC AGAGGCCACA GAGACCTCAG ATGAGGCTGA GCGCCCAAGC CCTCTACTCA GCCACATCCT CTTGGAACAC AACTATGCCC TAGCCATCAA GCCCCCGCCT ACCACACCAG CACCTCGGCC CCTGGAGCCA GCTCCTGCCC TTGCTGCACT CTTCAGCTCT CCAGCTGATG AAGTCTTAGA GGCCCCTGAG GTGGTGGTGG CTGAGGCAGA GGAGCCAAAG CAACAGCTGC AGCAGCAGCA TCCAGAGCAG GAAGGAGAAG AGGAGGAGGA AGATGAAGAG GAGGAGTCCG AGTCTTCAGA AAGCAGCAGC AGCAGCAGCA GTGATGAGGA AGGGGCAATC AGGAGGCGTA GCCTCCGCTC CCACACTCGA AGAAGAAGGC CACCACTCCC ACCCCCACCC CCACCTCCTC CTTCCTTTGA GCCGAGAAGT GAATTTGAGC AGATGACCAT CTTGTATGAT ATTTGGAACT CAGGCCTTGA CTTGGAAGAC ATGAGCTATT TGCGACTCAC ATATGAACGG CTGCTGCAGC AAACCAGTGG CGCTGACTGG CTTAATGACA CCCACTGGGT CCAGCACACA ATCACCAACC TAAGCACTCC AAAGCGCAAA AGACGGCCCC AAGATGGGCC CCGGGAGCAT CAGACAGGCT CTGCACGCAG CGAAGGTTAT TACCCCATTA GCAAGAAGGA GAAAGATAAA TACTTGGATG TGTGCCCCGT CTCGGCCCGG CAGCTGGAAG GTGGAGACAC TCAGGGGACT AATCGGGTGC TTTCTGAGCG ACGGTCAGAG CAGCGGCGGT TACTAAGCGC TATTGGCACC TCAGCCATCA TGGACAGTGA TCTGCTAAAG CTAAACCAGC TCAAGTTTCG GAAGAAGAAA CTCCGATTTG GCCGGAGCCG TATCCATGAG TGGGGTCTGT TTGCCATGGA ACCCATTGCC GCTGATGAGA TGGTGATCGA ATATGTTGGC CAGAACATCC GCCAGATGGT AGCGGACATG CGGGAAAAGC GCTACGTGCA GGAGGGCATT GGCAGCAGCT ACCTTTTCCG GGTGGATCAC GACACCATCA TTGATGCCAC CAAGTGTGGC AACCTCGCCA GGTTCATCAA CCACTGCTGC ACGCCCAACT GCTATGCGAA GGTGATCACC ATTGAGTCTC AGAAAAAGAT TGTCATCTAC TCGAAGCAGC CCATCGGCGT GGATGAGGAG ATCACCTACG ACTACAAGTT CCCACTAGAA GACAACAAGA TTCCGTGTCT GTGCGGCACT GAAAGTTGCC GTGGCTCCCT CAACGGATCC GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGTAGC GGCCGCGAAG GATCTGCGAT CGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC GGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GCTGAAGCTT CGAGGGGCTC GCATCTCTCC TTCACGCGCC CGCCGCCCTA CCTGAGGCCG CCATCCACGC CGGTTGAGTC GCGTTCTGCC GCCTCCCGCC TGTGGTGCCT CCTGAACTGC GTCCGCCGTC TAGGTAAGTT TAAAGCTCAG GTCGAGACCG GGCCTTTGTC CGGCGCTCCC TTGGAGCCTA CCTAGACTCA GCCGGCTCTC CACGCTTTGC CTGACCCTGC TTGCTCAACT CTACGTCTTT GTTTCGTTTT CTGTTCTGCG CCGTTACAGA TCCAAGCTGT GACCGGCGCC TACGCTAGAC GCCACCATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCAAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGGTGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGTGCTGGTG GGCAGCTTCG CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTT CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGCTGCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC CATCGCCTTC GCCAGATCCC GCGCTCAGTC GTCCAATTCT GCCGTGGACG GCACCGCCGG ACCCGGCTCC ACCGGATCTC GCGAGGGCAG AGGAAGTCTT CTAACATGCG GTGACGTGGA GGAGAATCCC GGCCCTATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGAAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTCCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGCCGCCTCC CCGCCTGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAAATAAG ATCTGCTTTT TGCTTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTAGTAGTT CATGTCATCT TATTATTCAG TATTTATAAC TTGCAAAGAA ATGAATATCA GAGAGTGAGA GGAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGCTCTAG CTATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GACTTTTGCA GAGACGGCCC AAATTCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT G
