NM_000834.5;DEE27; EIEE27; GluN2B; hNR3; MRD6; NMDAR2B;NR2B; NR3
100μg liquid plasmid(Instantly):$134
| name | PiggyBac-CMV-GRIN2B(human)-Puro | another name | aPiggyBac-CMV-GRIN2B(human)-Puro |
| Insert Size (bp) | 4455bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | EF1a-R：gagccagtacacgacatcac | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG ACACCTGGGG GGATACGGGG AAAAGGCCTC CAAGGCCTAC TAGTATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGATT CTAGAGCTAG CGAATTCGCC ACCATGAAGC CCAGAGCGGA GTGCTGTTCT CCCAAGTTCT GGTTGGTGTT GGCCGTCCTG GCCGTGTCAG GCAGCAGAGC TCGTTCTCAG AAGAGCCCCC CCAGCATTGG CATTGCTGTC ATCCTCGTGG GCACTTCCGA CGAGGTGGCC ATCAAGGATG CCCACGAGAA AGATGATTTC CACCATCTCT CCGTGGTACC CCGGGTGGAA CTGGTAGCCA TGAATGAGAC CGACCCAAAG AGCATCATCA CCCGCATCTG TGATCTCATG TCTGACCGGA AGATCCAGGG GGTGGTGTTT GCTGATGACA CAGACCAGGA AGCCATCGCC CAGATCCTCG ATTTCATTTC AGCACAGACT CTCACCCCCA TCCTGGGCAT CCACGGGGGC TCCTCTATGA TAATGGCAGA TAAGGATGAA TCCTCCATGT TCTTCCAGTT TGGCCCATCA ATTGAACAGC AAGCTTCCGT AATGCTCAAC ATCATGGAAG AATATGACTG GTACATCTTT TCTATCGTCA CCACCTATTT CCCTGGCTAC CAGGACTTTG TAAACAAGAT CCGCAGCACC ATTGAGAATA GCTTTGTGGG CTGGGAGCTA GAGGAGGTCC TCCTACTGGA CATGTCCCTG GACGATGGAG ATTCTAAGAT CCAGAATCAG CTCAAGAAAC TTCAAAGCCC CATCATTCTT CTTTACTGTA CCAAGGAAGA AGCCACCTAC ATCTTTGAAG TGGCCAACTC AGTAGGGCTG ACTGGCTATG GCTACACGTG GATCGTGCCC AGTCTGGTGG CAGGGGATAC AGACACAGTG CCTGCGGAGT TCCCCACTGG GCTCATCTCT GTATCATATG ATGAATGGGA CTATGGCCTC CCCGCCAGAG TGAGAGATGG AATTGCCATA ATCACCACTG CTGCTTCTGA CATGCTGTCT GAGCACAGCT TCATCCCTGA GCCCAAAAGC AGTTGTTACA ACACCCACGA GAAGAGAATC TACCAGTCCA ATATGCTAAA TAGGTATCTG ATCAATGTCA CTTTTGAGGG GAGGAATTTG TCCTTCAGTG AAGATGGCTA CCAGATGCAC CCGAAACTGG TGATAATTCT TCTGAACAAG GAGAGGAAGT GGGAAAGGGT GGGGAAGTGG AAAGACAAGT CCCTGCAGAT GAAGTACTAT GTGTGGCCCC GAATGTGTCC AGAGACTGAA GAGCAGGAGG ATGACCATCT GAGCATTGTG ACCCTGGAGG AGGCACCATT TGTCATTGTG GAAAGTGTGG ACCCTCTGAG TGGAACCTGC ATGAGGAACA CAGTCCCCTG CCAAAAACGC ATAGTCACTG AGAATAAAAC AGACGAGGAG CCGGGTTACA TCAAAAAATG CTGCAAGGGG TTCTGTATTG ACATCCTTAA GAAAATTTCT AAATCTGTGA AGTTCACCTA TGACCTTTAC CTGGTTACCA ATGGCAAGCA TGGGAAGAAA ATCAATGGAA CCTGGAATGG TATGATTGGA GAGGTGGTCA TGAAGAGGGC CTACATGGCA GTGGGCTCAC TCACCATCAA TGAGGAACGA TCGGAGGTGG TCGACTTCTC TGTGCCCTTC ATAGAGACAG GCATCAGTGT CATGGTGTCA CGCAGCAATG GGACTGTCTC ACCTTCTGCC TTCTTAGAGC CATTCAGCGC TGACGTATGG GTGATGATGT TTGTGATGCT GCTCATCGTC TCAGCCGTGG CTGTCTTTGT CTTTGAGTAC TTCAGCCCTG TGGGTTATAA CAGGTGCCTC GCTGATGGCA GAGAGCCTGG TGGACCCTCT TTCACCATCG GCAAAGCTAT TTGGTTGCTC TGGGGTCTGG TGTTTAACAA CTCCGTACCT GTGCAGAACC CAAAGGGGAC CACCTCCAAG ATCATGGTGT CAGTGTGGGC CTTCTTTGCT GTCATCTTCC TGGCCAGCTA CACTGCCAAC TTAGCTGCCT TCATGATCCA AGAGGAATAT GTGGACCAGG TTTCTGGCCT GAGCGACAAA AAGTTCCAGA GACCTAATGA CTTCTCACCC CCTTTCCGCT TTGGGACCGT GCCCAACGGC AGCACAGAGA GAAATATTCG CAATAACTAT GCAGAAATGC ATGCCTACAT GGGAAAGTTC AACCAGAGGG GTGTAGATGA TGCATTGCTC TCCCTGAAAA CAGGGAAACT GGATGCCTTC ATCTATGATG CAGCAGTGCT GAACTATATG GCAGGCAGAG ATGAAGGCTG CAAGCTGGTG ACCATTGGCA GTGGGAAGGT CTTTGCTTCC ACTGGCTATG GCATTGCCAT CCAAAAAGAT TCTGGGTGGA AGCGCCAGGT GGACCTTGCT ATCCTGCAGC TCTTTGGAGA TGGGGAGATG GAAGAACTGG AAGCTCTCTG GCTCACTGGC ATTTGTCACA ATGAGAAGAA TGAGGTCATG AGCAGCCAGC TGGACATTGA CAACATGGCA GGGGTCTTCT ACATGTTGGG GGCGGCCATG GCTCTCAGCC TCATCACCTT CATCTGCGAA CACCTTTTCT ATTGGCAGTT CCGACATTGC TTTATGGGTG TCTGTTCTGG CAAGCCTGGC ATGGTCTTCT CCATCAGCAG AGGTATCTAC AGCTGCATCC ATGGGGTGGC GATCGAGGAG CGCCAGTCTG TAATGAACTC CCCCACCGCA ACCATGAACA ACACACACTC CAACATCCTG CGCCTGCTGC GCACGGCCAA GAACATGGCT AACCTGTCTG GTGTGAATGG CTCACCGCAG AGCGCCCTGG ACTTCATCCG ACGGGAGTCA TCCGTCTATG ACATCTCAGA GCACCGCCGC AGCTTCACGC ATTCTGACTG CAAATCCTAC AACAACCCGC CCTGTGAGGA GAACCTCTTC AGTGACTACA TCAGTGAGGT AGAGAGAACG TTCGGGAACC TGCAGCTGAA GGACAGCAAC GTGTACCAAG ATCACTACCA CCATCACCAC CGGCCCCATA GTATTGGCAG TGCCAGCTCC ATCGATGGGC TCTACGACTG TGACAACCCA CCCTTCACCA CCCAGTCCAG GTCCATCAGC AAGAAGCCCC TGGACATCGG CCTCCCCTCC TCCAAGCACA GCCAGCTCAG TGACCTGTAC GGCAAATTCT CCTTCAAGAG CGACCGCTAC AGTGGCCACG ACGACTTGAT CCGCTCCGAT GTCTCTGACA TCTCAACCCA CACCGTCACC TATGGGAACA TCGAGGGCAA TGCCGCCAAG AGGCGTAAGC AGCAATATAA GGACAGCCTG AAGAAGCGGC CTGCCTCGGC CAAGTCCCGC AGGGAGTTTG ACGAGATCGA GCTGGCCTAC CGTCGCCGAC CGCCCCGCTC CCCTGACCAC AAGCGCTACT TCAGGGACAA GGAAGGGCTA CGGGACTTCT ACCTGGACCA GTTCCGAACA AAGGAGAACT CACCCCACTG GGAGCACGTA GACCTGACCG ACATCTACAA GGAGCGGAGT GATGACTTTA AGCGCGACTC CGTCAGCGGA GGAGGGCCCT GTACCAACAG GTCTCACATC AAGCACGGGA CGGGCGACAA ACACGGCGTG GTCAGCGGGG TACCTGCACC TTGGGAGAAG AACCTGACCA ACGTGGAGTG GGAGGACCGG TCCGGGGGCA ACTTCTGCCG CAGCTGTCCC TCCAAGCTGC ACAACTACTC CACGACGGTG ACGGGTCAGA ACTCGGGCAG GCAGGCGTGC ATCCGGTGTG AGGCTTGCAA GAAAGCAGGC AACCTGTATG ACATCAGTGA GGACAACTCC CTGCAGGAAC TGGACCAGCC GGCTGCCCCA GTGGCGGTGA CGTCAAACGC CTCCACCACT AAGTACCCTC AGAGCCCGAC TAATTCCAAG GCCCAGAAGA AGAACCGGAA CAAACTGCGC CGGCAGCACT CCTACGACAC CTTCGTGGAC CTGCAGAAGG AAGAAGCCGC CCTGGCCCCG CGCAGCGTAA GCCTGAAAGA CAAGGGCCGA TTCATGGATG GGAGCCCCTA CGCCCACATG TTTGAGATGT CAGCTGGCGA GAGCACCTTT GCCAACAACA AGTCCTCAGT GCCCACTGCC GGACATCACC ACCACAACAA CCCCGGCGGC GGGTACATGC TCAGCAAGTC GCTCTACCCT GACCGGGTCA CGCAAAACCC TTTCATCCCC ACTTTTGGGG ACGACCAGTG CTTGCTCCAT GGCAGCAAAT CCTACTTCTT CAGGCAGCCC ACGGTGGCGG GGGCGTCGAA AGCCAGGCCG GACTTCCGGG CCCTTGTCAC CAACAAGCCG GTGGTCTCGG CCCTTCATGG GGCCGTGCCA GCCCGTTTCC AGAAGGACAT CTGTATAGGG AACCAGTCCA ACCCCTGTGT GCCTAACAAC AAAAACCCCA GGGCTTTCAA TGGCTCCAGC AATGGGCATG TTTATGAGAA ACTTTCTAGT ATTGAGTCTG ATGTCTGAGC GGCCGCGAAG GATCTGCGAT CGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC GGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GCTGAAGCTT CGAGGGGCTC GCATCTCTCC TTCACGCGCC CGCCGCCCTA CCTGAGGCCG CCATCCACGC CGGTTGAGTC GCGTTCTGCC GCCTCCCGCC TGTGGTGCCT CCTGAACTGC GTCCGCCGTC TAGGTAAGTT TAAAGCTCAG GTCGAGACCG GGCCTTTGTC CGGCGCTCCC TTGGAGCCTA CCTAGACTCA GCCGGCTCTC CACGCTTTGC CTGACCCTGC TTGCTCAACT CTACGTCTTT GTTTCGTTTT CTGTTCTGCG CCGTTACAGA TCCAAGCTGT GACCGGCGCC TACGCTAGAC GCCACCATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGAAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGAATTG ACTCAAATGA TGTCAATTAG TCTATCAGAA GCTATCTGGT CTCCCTTCCG GGGGACAAGA CATCCCTGTT TAATATTTAA ACAGCAGTGT TCCCAAACTG GGTTCTTATA TCCCTTGCTC TGGTCAACCA GGTTGCAGGG TTTCCTGTCC TCACAGGAAC GAAGTCCCTA AAGAAACAGT GGCAGCCAGG TTTAGCCCCG GAATTGACTG GATTCCTTTT TTAGGGCCCA TTGGTATGGC TTTTTCCCCG TATCCCCCCA GGTGTCTGCA GGCTCAAAGA GCAGCGAGAA GCGTTCAGAG GAAAGCGATC CCGTGCCACC TTCCCCGTGC CCGGGCTGTC CCCGCACGCT GCCGGCTCGG GGATGCGGGG GGAGCGCCGG ACCGGAGCGG AGCCCCGGGC GGCTCGCTGC TGCCCCCTAG CGGGGGAGGG ACGTAATTAC ATCCCTGGGG GCTTTGGGGG GGGGCTGTCC CTGATATCTA TAACAAGAAA ATATATATAT AATAAGTTAT CACGTAAGTA GAACATGAAA TAACAATATA ATTATCGTAT GAGTTAAATC TTAAAAGTCA CGTAAAAGAT AATCATGCGT CATTTTGACT CACGCGGTCG TTATAGTTCA AAATCAGTGA CACTTACCGC ATTGACAAGC ACGCCTCACG GGAGCTCCAA GCGGCGACTG AGATGTCCTA AATGCACAGC GACGGATTCG CGCTATTTAG AAAGAGAGAG CAATATTTCA AGAATGCATG CGTCAATTTT ACGCAGACTA TCTTTCTAGG GTTAATCTAG CTGCATCAGG ATCATATCGT CGGGTCTTTT TTCCGGCTCA GTCATCGCCC AAGCTGGCGC TATCTGGGCA TCGGGGAGGA AGAAGCCCGT GCCTTTTCCC GCGAGGTTGA AGCGGCATGG AAAGAGTTTG CCGAGGATGA CTGCTGCTGC ATTGACGTTG AGCGAAAACG CACGTTTACC ATGATGATTC GGGAAGGTGT GGCCATGCAC GCCTTTAACG GTGAACTGTT CGTTCAGGCC ACCTGGGATA CCAGTTCGTC GCGGCTTTTC CGGACACAGT TCCGGATGGT CAGCCCGAAG CGCATCAGCA ACCCGAACAA TACCGGCGAC AGCCGGAACT GCCGTGCCGG TGTGCAGATT AATGACAGCG GTGCGGCGCT GGGATATTAC GTCAGCGAGG ACGGGTATCC TGGCTGGATG CCGCAGAAAT GGACATGGAT ACCCCGTGAG TTACCCGGCG GGCGCGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CAT
