NM_000744.7;BFNC; EBN; EBN1; NACHR; NACHRA4; NACRA4
100μg liquid plasmid(Instantly):$91
| name | PiggyBac-CMV-CHRNA4(human)-Neo | another name | aPiggyBac-CMV-CHRNA4(human)-Neo |
| Insert Size (bp) | 1884bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | EF1a-R：gagccagtacacgacatcac | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG ACACCTGGGG GGATACGGGG AAAAGGCCTC CAAGGCCTAC TAGTATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGATT CTAGAGCTAG CGAATTCGCC ACCATGGAGC TAGGGGGCCC CGGAGCGCCG CGGCTGCTGC CGCCGCTGCT GCTGCTTCTG GGGACCGGCC TCCTGCGCGC CAGCAGCCAT GTGGAGACCC GGGCCCACGC CGAGGAGCGG CTCCTGAAGA AACTCTTCTC CGGTTACAAC AAGTGGTCCC GACCCGTGGC CAACATCTCG GACGTGGTCC TCGTCCGCTT CGGCCTGTCC ATCGCTCAGC TCATTGACGT GGATGAGAAG AACCAGATGA TGACCACGAA CGTATGGGTG AAGCAGGAGT GGCACGACTA CAAGCTGCGC TGGGACCCAG CTGACTATGA GAATGTCACC TCCATCCGCA TCCCCTCCGA GCTCATCTGG CGGCCGGACA TCGTCCTCTA CAACAATGCT GACGGGGACT TCGCGGTCAC CCACCTGACC AAGGCCCACC TGTTCCATGA CGGGCGGGTG CAGTGGACTC CCCCGGCCAT TTACAAGAGC TCCTGCAGCA TCGACGTCAC CTTCTTCCCC TTCGACCAGC AGAACTGCAC CATGAAATTC GGCTCCTGGA CCTACGACAA GGCCAAGATC GACCTGGTGA ACATGCACAG CCGCGTGGAC CAGCTGGACT TCTGGGAGAG TGGCGAGTGG GTCATCGTGG ATGCCGTGGG CACCTACAAC ACCAGGAAGT ACGAGTGCTG TGCCGAGATC TACCCGGACA TCACCTATGC CTTCGTCATC CGGCGGCTGC CGCTCTTCTA CACCATCAAC CTCATCATCC CCTGCCTGCT CATCTCCTGC CTCACCGTGC TGGTCTTCTA CCTGCCCTCC GAGTGTGGCG AGAAGATCAC GCTGTGCATC TCCGTGCTGC TGTCGCTCAC CGTCTTCCTG CTGCTCATCA CCGAGATCAT CCCGTCCACC TCACTGGTCA TCCCACTCAT CGGCGAGTAC CTGCTGTTCA CCATGATCTT CGTCACCCTG TCCATCGTCA TCACGGTCTT CGTGCTCAAC GTGCACCACC GCTCGCCACG CACGCACACC ATGCCCACCT GGGTACGCAG GGTCTTCCTG GACATCGTGC CACGCCTGCT CCTCATGAAG CGGCCGTCCG TGGTCAAGGA CAATTGCCGG CGGCTCATCG AGTCCATGCA TAAGATGGCC AGTGCCCCGC GCTTCTGGCC CGAGCCAGAA GGGGAGCCCC CTGCCACGAG CGGCACCCAG AGCCTGCACC CGCCCTCACC GTCCTTCTGT GTCCCCCTGG ATGTGCCGGC TGAGCCTGGG CCTTCCTGCA AGTCACCCTC CGACCAGCTC CCTCCTCAGC AGCCCCTGGA AGCTGAGAAA GCCAGCCCCC ACCCCTCGCC TGGACCCTGC CGCCCGCCCC ACGGCACCCA GGCACCAGGG CTGGCCAAAG CCAGGTCCCT CAGCGTCCAG CACATGTCCA GCCCTGGCGA AGCGGTGGAA GGCGGCGTCC GGTGCCGGTC TCGGAGCATC CAGTACTGTG TTCCCCGAGA CGATGCCGCC CCCGAGGCAG ATGGCCAGGC TGCCGGCGCC CTGGCCTCTC GCAACACCCA CTCGGCTGAG CTCCCACCCC CAGACCAGCC CTCTCCGTGC AAATGCACAT GCAAGAAGGA GCCCTCTTCG GTGTCCCCGA GCGCCACGGT CAAGACCCGC AGCACCAAAG CGCCGCCCCC GCACCTGCCC CTGTCGCCGG CCCTGACCCG GGCGGTGGAG GGCGTCCAGT ACATTGCAGA CCACCTGAAG GCCGAAGACA CAGACTTCTC GGTGAAGGAG GACTGGAAGT ACGTGGCCAT GGTCATCGAC CGCATCTTCC TCTGGATGTT CATCATCGTC TGCCTGCTGG GGACGGTGGG CCTCTTCCTG CCGCCCTGGC TGGCTGGCAT GATCTAGGCG GCCGCGAAGG ATCTGCGATC GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGAACG GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG CTGAAGCTTC GAGGGGCTCG CATCTCTCCT TCACGCGCCC GCCGCCCTAC CTGAGGCCGC CATCCACGCC GGTTGAGTCG CGTTCTGCCG CCTCCCGCCT GTGGTGCCTC CTGAACTGCG TCCGCCGTCT AGGTAAGTTT AAAGCTCAGG TCGAGACCGG GCCTTTGTCC GGCGCTCCCT TGGAGCCTAC CTAGACTCAG CCGGCTCTCC ACGCTTTGCC TGACCCTGCT TGCTCAACTC TACGTCTTTG TTTCGTTTTC TGTTCTGCGC CGTTACAGAT CCAAGCTGTG ACCGGCGCCT ACGCTAGGCC ACCATGGCTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGT CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CGTTAACTAA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GAATTGACTC AAATGATGTC AATTAGTCTA TCAGAAGCTA TCTGGTCTCC CTTCCGGGGG ACAAGACATC CCTGTTTAAT ATTTAAACAG CAGTGTTCCC AAACTGGGTT CTTATATCCC TTGCTCTGGT CAACCAGGTT GCAGGGTTTC CTGTCCTCAC AGGAACGAAG TCCCTAAAGA AACAGTGGCA GCCAGGTTTA GCCCCGGAAT TGACTGGATT CCTTTTTTAG GGCCCATTGG TATGGCTTTT TCCCCGTATC CCCCCAGGTG TCTGCAGGCT CAAAGAGCAG CGAGAAGCGT TCAGAGGAAA GCGATCCCGT GCCACCTTCC CCGTGCCCGG GCTGTCCCCG CACGCTGCCG GCTCGGGGAT GCGGGGGGAG CGCCGGACCG GAGCGGAGCC CCGGGCGGCT CGCTGCTGCC CCCTAGCGGG GGAGGGACGT AATTACATCC CTGGGGGCTT TGGGGGGGGG CTGTCCCTGA TATCTATAAC AAGAAAATAT ATATATAATA AGTTATCACG TAAGTAGAAC ATGAAATAAC AATATAATTA TCGTATGAGT TAAATCTTAA AAGTCACGTA AAAGATAATC ATGCGTCATT TTGACTCACG CGGTCGTTAT AGTTCAAAAT CAGTGACACT TACCGCATTG ACAAGCACGC CTCACGGGAG CTCCAAGCGG CGACTGAGAT GTCCTAAATG CACAGCGACG GATTCGCGCT ATTTAGAAAG AGAGAGCAAT ATTTCAAGAA TGCATGCGTC AATTTTACGC AGACTATCTT TCTAGGGTTA ATCTAGCTGC ATCAGGATCA TATCGTCGGG TCTTTTTTCC GGCTCAGTCA TCGCCCAAGC TGGCGCTATC TGGGCATCGG GGAGGAAGAA GCCCGTGCCT TTTCCCGCGA GGTTGAAGCG GCATGGAAAG AGTTTGCCGA GGATGACTGC TGCTGCATTG ACGTTGAGCG AAAACGCACG TTTACCATGA TGATTCGGGA AGGTGTGGCC ATGCACGCCT TTAACGGTGA ACTGTTCGTT CAGGCCACCT GGGATACCAG TTCGTCGCGG CTTTTCCGGA CACAGTTCCG GATGGTCAGC CCGAAGCGCA TCAGCAACCC GAACAATACC GGCGACAGCC GGAACTGCCG TGCCGGTGTG CAGATTAATG ACAGCGGTGC GGCGCTGGGA TATTACGTCA GCGAGGACGG GTATCCTGGC TGGATGCCGC AGAAATGGAC ATGGATACCC CGTGAGTTAC CCGGCGGGCG CGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCAT
