NM_177328.3;6330570A01Rik; C030018L03; E130018M02Rik;Gpr1g; Gprc1g; mGluR7; SMN2; Tg(SMN2)89Ahmb
100μg liquid plasmid(Instantly):$105
| name | pLV3-SFFV-Grm7(mouse)-FLAG-Puro | another name | apLV3-SFFV-Grm7(mouse)-FLAG-Puro |
| Insert Size (bp) | 2748bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | SFFV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGC GCGTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC AGGGACTTGA AAGCGAAAGG GAAACCAGAG GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA CGGCAAGAGG CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT AGAAGGAGAG AGATGGGTGC GAGAGCGTCA GTATTAAGCG GGGGAGAATT AGATCGCGAT GGGAAAAAAT TCGGTTAAGG CCAGGGGGAA AGAAAAAATA TAAATTAAAA CATATAGTAT GGGCAAGCAG GGAGCTAGAA CGATTCGCAG TTAATCCTGG CCTGTTAGAA ACATCAGAAG GCTGTAGACA AATACTGGGA CAGCTACAAC CATCCCTTCA GACAGGATCA GAAGAACTTA GATCATTATA TAATACAGTA GCAACCCTCT ATTGTGTGCA TCAAAGGATA GAGATAAAAG ACACCAAGGA AGCTTTAGAC AAGATAGAGG AAGAGCAAAA CAAAAGTAAG ACCACCGCAC AGCAAGCGGC CGCTGATCTT CAGACCTGGA GGAGGAGATA TGAGGGACAA TTGGAGAAGT GAATTATATA AATATAAAGT AGTAAAAATT GAACCATTAG GAGTAGCACC CACCAAGGCA AAGAGAAGAG TGGTGCAGAG AGAAAAAAGA GCAGTGGGAA TAGGAGCTTT GTTCCTTGGG TTCTTGGGAG CAGCAGGAAG CACTATGGGC GCAGCGTCAA TGACGCTGAC GGTACAGGCC AGACAATTAT TGTCTGGTAT AGTGCAGCAG CAGAACAATT TGCTGAGGGC TATTGAGGCG CAACAGCATC TGTTGCAACT CACAGTCTGG GGCATCAAGC AGCTCCAGGC AAGAATCCTG GCTGTGGAAA GATACCTAAA GGATCAACAG CTCCTGGGGA TTTGGGGTTG CTCTGGAAAA CTCATTTGCA CCACTGCTGT GCCTTGGAAT GCTAGTTGGA GTAATAAATC TCTGGAACAG ATTTGGAATC ACACGACCTG GATGGAGTGG GACAGAGAAA TTAACAATTA CACAAGCTTA ATACACTCCT TAATTGAAGA ATCGCAAAAC CAGCAAGAAA AGAATGAACA AGAATTATTG GAATTAGATA AATGGGCAAG TTTGTGGAAT TGGTTTAACA TAACAAATTG GCTGTGGTAT ATAAAATTAT TCATAATGAT AGTAGGAGGC TTGGTAGGTT TAAGAATAGT TTTTGCTGTA CTTTCTATAG TGAATAGAGT TAGGCAGGGA TATTCACCAT TATCGTTTCA GACCCACCTC CCAACCCCGA GGGGACCCGA CAGGCCCGAA GGAATAGAAG AAGAAGGTGG AGAGAGAGAC AGAGACAGAT CCATTCGATT AGTGAACGGA TCGGCACTGC GTGCGCCAAT TCTGCAGACA AATGGCAGTA TTCATCCACA ATTTTAAAAG AAAAGGGGGG ATTGGGGGGT ACAGTGCAGG GGAAAGAATA GTAGACATAA TAGCAACAGA CATACAAACT AAAGAATTAC AAAAACAAAT TACAAAAATT CAAAATTTTC GGGTTTATTA CAGGGACAGC AGAGATCCAG TTTGGTTAAT TAAGGTACCC GGGAGGTGGT CCCTGCAGTT ACGCCAATGA TAACCCCCGC CAGAAAAATC TTAGTAGCCT TCCCTTTTTG TTTTCCGTGC CCCAACTCGG CGGATTGACT CGGCCCCTTC CGGAAACACC CGAATCAACT TCTAGTCAAA TTATTGTTCA CGCCGCAATG ACCCACCCCT GGCCCGCGTC TGTGGAACTG ACCCCTGGTG TACAGGAGAG TTCGCTGCTG AAAGTGGTCC CAAAGGGGTA CTAGTTTTTA AGCTCCCAAC TCCCCCTCCC CCAGCGTCTG GAGGATTCCA CACCCTCGCA CCGCAGGGGC GAGGAAGTGG GCGGAGTCCG GTTTTGGCGC CAGCCGCTGA GGCTGCCAAG CAGAAAAGCC ACCGCTGAGG AGACTCCGGT CACTGTCCTC GCCCCGCCTC CCCCTTCCCT CCCCTTGGGG ACCACCGGGC GCCACGCCGC GAACGTTAAG TGCCGCGGTC GTCGGCGCCT CCGCCCTCCC CCTTGGGCCC CAATTCCCAG CGGGCGCGGC GCGCGGCCCC TCCCCCCGCC GGGCGCGCGC CCGCTGCCCC GCCCTTCGTG GCCGCCCGGC GTGGGCGGTG CCACCCCTCC CCCCGGCGGC CCCGCGCGCA GCTCCCGGCT CCCTCCCCCT TCGGATGTGG CTTGAGCTGT AGGCGCGGAG GCTAGCTGCA GTAACGCCAT TTTGCAAGGC ATGGAAAAAT ACCAAACCAA GAATAGAGAA GTTCAGATCA AGGGCGGGTA CATGAAAATA GCTAACGTTG GGCCAAACAG GATATCTGCG GTGAGCAGTT TCGGCCCCGG CCCGGGGCCA AGAACAGATG GTCACCGCAG TTTCGGCCCC GGCCCGAGGC CAAGAACAGA TGGTCCCCAG ATATGGCCCA ACCCTCAGCA GTTTCTTAAG ACCCATCAGA TGTTTCCAGG CTCCCCCAAG GACCTGAAAT GACCCTGCGC CTTATTTGAA TTAACCAATC AGCCTGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTTCCCG AGCTCTATAA AAGAGCTCAC AACCCCTCAC TCGGCGCGCC AGTCCTCCGA CAGACTGAGT CGCCCGGGGG AATTCGCCAC CATGGTCCAG CTGGGGAAGC TGCTCCGCGT CCTGACTTTG ATGAAGTTCC CCTGCTGCGT GCTGGAGGTG CTCCTGTGCG TGCTGGCGGC GGCGGCGCGC GGCCAGGAGA TGTACGCCCC GCACTCGATC CGGATCGAGG GGGACGTCAC CCTTGGGGGG CTGTTCCCAG TGCACGCCAA GGGTCCCAGC GGAGTGCCCT GCGGCGACAT CAAGAGGGAG AATGGGATCC ACAGGCTGGA AGCTATGCTT TATGCTCTCG ACCAGATCAA CAGCGATCCC AACCTGCTGC CCAACGTAAC GTTAGGCGCG CGGATCCTGG ACACTTGTTC CAGGGACACT TATGCGCTCG AACAGTCGCT CACTTTCGTC CAGGCGCTTA TCCAGAAGGA CACCTCCGAC GTGCGTTGCA CCAACGGAGA GCCCCCGGTT TTCGTCAAGC CAGAGAAAGT AGTTGGAGTG ATTGGGGCTT CGGGGAGCTC CGTCTCCATC ATGGTAGCCA ACATCTTGAG GCTTTTCCAG ATACCTCAGA TTAGTTATGC ATCCACGGCT CCTGAACTCA GTGATGACCG TCGCTATGAC TTCTTCTCTC GCGTGGTCCC ACCTGATTCC TTCCAAGCCC AAGCTATGGT AGACATTGTA AAGGCATTGG GCTGGAATTA TGTATCTACT CTTGCATCTG AAGGAAGCTA TGGAGAGAAA GGTGTGGAGT CCTTCACACA AATTTCCAAA GAAGCAGGTG GACTCTGCAT TGCCCAGTCC GTGAGAATTC CCCAAGAACG CAAAGACAGG ACCATTGACT TTGATAGAAT TATCAAACAG CTCTTGGACA CCCCCAACTC CAGGGCTGTC GTGATTTTTG CCAACGATGA GGATATAAAG CAGATCCTTG CAGCAGCCAA AAGAGCTGAC CAAGTAGGAC ATTTTCTCTG GGTTGGGTCA GACAGCTGGG GTTCCAAAAT CAACCCACTG CATCAGCATG AAGATATTGC AGAAGGAGCC ATCACCATCC AACCTAAGAG AGCGACTGTG GAAGGATTTG ATGCTTACTT CACATCCCGG ACACTTGAAA ACAACAGGAG AAATGTATGG TTTGCCGAAT ACTGGGAAGA AAACTTCAAC TGCAAGTTGA CAATTAGTGG ATCCAAAAAA GAAGACACGG ATCGCAAATG CACAGGACAG GAGCGAATTG GAAAAGACTC TAATTATGAG CAGGAGGGTA AAGTACAGTT TGTGATCGAT GCTGTCTATG CCATGGCACA TGCCCTTCAT CACATGAATA AGGATCTGTG TGCTGACTAC CGGGGAGTGT GCCCAGAAAT GGAGCAAGCA GGGGGCAAGA AGTTGTTGAA GTATATCCGC AATGTTAACT TCAATGGTAG TGCTGGAACC CCAGTGATGT TTAACAAAAA TGGTGATGCT CCAGGACGAT ATGACATCTT CCAGTACCAG ACAACAAACA CTACCAATCC TGGTTATCGT CTCATTGGGC AGTGGACAGA TGAACTGCAG CTCAATATAG AGGACATGCA GTGGGGCAAA GGAGTCCGGG AGATCCCACC CTCTGTGTGC ACATTGCCAT GCAAGCCTGG GCAGAGGAAG AAGACACAGA AGGGAACCCC TTGCTGCTGG ACTTGTGAGC CCTGTGATGG ATACCAGTAT CAATTTGATG AGATGACATG TCAGCATTGT CCCTATGACC AGAGGCCCAA TGAGAACCGA ACTGGCTGTC AGAACATCCC AATCATCAAA CTGGAGTGGC ACTCCCCCTG GGCTGTAATT CCTGTCTTCC TGGCAATGTT GGGGATCATT GCCACCATCT TTGTCATGGC AACCTTCATC CGCTACAATG ATACACCCAT CGTCAGGGCA TCTGGGCGGG AACTCAGCTA TGTTTTATTG ACAGGCATCT TTCTCTGCTA TATCATCACC TTCCTAATGA TTGCCAAGCC AGATGTGGCA GTGTGTTCCT TCCGACGTGT CTTCTTGGGG TTGGGTATGT GTATTAGTTA TGCAGCCCTT TTAACAAAAA CCAATCGGAT TTATCGCATA TTCGAGCAGG GCAAGAAATC GGTGACAGCT CCCAGACTCA TAAGCCCAAC ATCACAACTG GCAATTACCT CCAGTTTAAT ATCGGTGCAA CTTCTAGGTG TCTTTATTTG GTTTGGGGTT GACCCACCCA ACATCATCAT AGACTACGAT GAGCATAAGA CAATGAACCC AGAACAAGCC AGGGGAGTTC TCAAATGTGA CATCACAGAC CTTCAGATCA TTTGTTCTTT GGGATATAGC ATTCTTCTCA TGGTCACATG TACTGTGTAT GCCATCAAGA CTCGGGGTGT ACCAGAGAAT TTTAATGAAG CCAAGCCCAT TGGATTCACT ATGTACACGA CTTGTATCGT ATGGCTTGCC TTCATCCCAA TATTTTTTGG CACAGCGCAA TCAGCAGAAA AGCTCTACAT ACAAACCACC ACGCTTACAA TCTCCATGAA CCTAAGTGCA TCAGTGGCGC TGGGGATGCT ATACATGCCG AAAGTGTACA TCATCATTTT CCACCCTGAA CTCAATGTCC AGAAACGGAA GCGAAGCTTC AAGGCCGTAG TCACAGCAGC CACCATGTCA TCGAGGCTGT CACACAAACC CAGTGACAGG CCCAATGGTG AGGCAAAGAC AGAACTTTGT GAAAATGTAG ACCCAAACAG CCCTGCTGCA AAAAAGAAGT ATGTCAGTTA TAATAACCTG GTTATCTCTA GAGATTACAA AGACGATGAC GATAAGGGAT CCGGCGCAAC AAACTTCTCT CTGCTGAAAC AAGCCGGAGA TGTCGAAGAG AATCCTGGAC CGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGAGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAA CGCGTTAAGT CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCGTCGACT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATC TGCTTTTTGC TTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATCTGC TCCCTGCTTG TGTGTTGGAG GTCGCTGAGT AGTGCGCGAG CAAAATTTAA GCTACAACAA GGCAAGGCTT GACCGACAAT TGCATGAAGA ATCTGCTTAG GGTTAGGCGT TTTGCGCTGC TTCGCGATGT ACGGGCCAGA TATACGCGTT GACATTGATT ATTGACTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG G
