pRSF-pLtetO-GFP-Cam
$84.00
In stock
SKU
PL41677
100μg liquid plasmid(Instantly):$127
| name | pRSF-pLtetO-GFP-Cam | another name | apRSF-pLtetO-GFP-Cam |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Chl | |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | LtetO | Replicon | RSF |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCCCTATCAG TGATAGAGAT TGACATCCCT ATCAGTGATA GAGATACTGA GCACATCAGC AGGACACACT GACCGAATTC ATTAAAGAGG AGAAAGGTAC CATGAGTAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA ATTCTTGTTG AATTAGATGG TGATGTTAAT GGGCACAAAT TTTCTGTCAG TGGAGAGGGT GAAGGTGATG CAACATACGG AAAACTTACC CTTAAATTTA TTTGCACTAC TGGAAAACTA CCTGTTCCAT GGCCAACACT TGTCACTACT TTCGGTTATG GTGTTCAATG CTTTGCGAGA TACCCAGATC ATATGAAACA GCATGACTTT TTCAAGAGTG CCATGCCCGA AGGTTATGTA CAGGAAAGAA CTATATTTTT CAAAGATGAC GGGAACTACA AGACACGTGC TGAAGTCAAG TTTGAAGGTG ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC TTGGACACAA ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC AAAAGAATGG AATCAAAGTT AACTTCAAAA TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC ACATGGCATG GATGAACTAT ACAAATAAAA GCTTGATATC GAATTCCTGC AGCCCGGGGG ATCCCATGGT ACGCGTGCTA GAGGCATCAA ATAAAACGAA AGGCTCAGTC GAAAGACTGG GCCTTTCGTT TTATGCGGCC GCGATCAAGC AGGTGCGACA GACGTCATAC TAGATATCAA GCGACTTCTC CTATCCCCTG GGAACACATC AATCTCACCG GAGAATATCG CTGGCCAAAG CCTTAGCGTA GGATTCCGCC CCTTCCCGCA AACGACCCCA AACAGGAAAC GCAGCTGAAA CGGGAAGCTC AACACCCACT GACGCATGGG TTGTTCAGGC AGTACTTCAT CAACCAGCAA GGCGGCACTT TCGGCCATCC GCCGCGCCCC ACAGCTCGGG CAGAAACCGC GACGCTTACA GCTGAAAGCG ACCAGGTGCT CGGCGTGGCA AGACTCGCAG CGAACCCGTA GAAAGCCATG CTCCAGCCGC CCGCATTGGA GAAATTCTTC AAATTCCCGT TGCACATAGC CCGGCAATTC CTTTCCCTGC TCTGCCATAA GCGCAGCGAA TGCCGGGTAA TACTCGTCAA CGATCTGATA GAGAAGGGTT TGCTCGGGTC GGTGGCTCTG GTAACGACCA GTATCCCGAT CCCGGCTGGC CGTCCTGGCC GCCACATGAG GCATGTTCCG CGTCCTTGCA ATACTGTGTT TACATACAGT CTATCGCTTA GCGGAAAGTT CTTTTACCCT CAGCCGAAAT GCCTGCCGTT GCTAGACATT GCCAGCCAGT GCCCGTCACT CCCGTACTAA CTGTCACGAA CCCCTGCAAT AACTGTCACG CCCCCCTGCA ATAACTGTCA CGAACCCCTG CAATAACTGT CACGCCCCCA AACCTGCAAA CCCAGCAGGG GCGGGGGCTG GCGGGGTGTT GGAAAAATCC ATCCATGATT ATCTAAGAAT AATCCACTAG GCGCGGTTAT CAGCGCCCTT GTGGGGCGCT GCTGCCCTTG CCCAATATGC CCGGCCAGAG GCCGGATAGC TGGTCTATTC GCTGCGCTAG GCTACACACC GCCCCACCGC TGCGCGGCAG GGGGAAAGGC GGGCAAAGCC CGCTAAACCC CACACCAAAC CCCGCAGAAA TACGCTGGAG CGCTTTTAGC CGCTTTAGCG GCCTTTCCCC CTACCCGAAG GGTGGGGGCG CGTGTGCAGC CCCGCAGGGC CTGTCTCGGT CGATCATTCA GCCCGGCTCA TCCTTCTGGC GTGGCGGCAG ACCGAACAAG GCGCGGTCGT GGTCGCGTTC AAGGTACGCA TCCATTGCCG CCATGAGCCG ATCCTCCGGC CACTCGCTGC TGTTCACCTT GGCCAAAATC ATGGCCCCCA CCAGCACCCT GCGCCTTGTT TCGTTCTTGC GCTCTTGCTG CTGTTCCCTT GCCCGCACCC GCTGAATTTC GGCATTGATT CGCGCTCGTT GTTCTTCGAG CTTGGCCAGC CGATCCGCCG CCTTGTTGCT CCCCTTAACC ATCTTGACAC CCCATTGTTA ATGTGCTGTC TCGTAGGCTA TCATGGAGGC ACAGCGGCGG CAATCCCGAC CCTACTTTGT AGGGGAGGGG CACTTACCGG TTTCTCTTCG AGAAACTGGC CTAACGGCCA CCCTTCGGGC GGTGCGCTCT CCGAGGGCCA TTGCATGGAG CCGAAAAGCA AAAGCAACAG CGAGGCAGCA TGGCGATTTA TCACCTTACG GCGAAAACCG GCAGCAGGTC GGGCGGCCAA TCGGCCAGGG CCAAGGCCGA CTACATCCAG CGCGAAGGCA AGTATGCCCG CGACATGGAT GAAGTCTTGC ACGCCGAATC CGGGCACATG CCGGAGTTCG TCGAGCGGCC CGCCGACTAC TGGGATGCTG CCGACCTGTA TGAACGCGCC AATGGGCGGC TGTTCAAGGA GGTCGAATTT GCCCTGCCGG TCGAGCTGAC CCTCGACCAG CAGAAGGCGC TGGCGTCCGA GTTCGCCCAG CACCTGACCG GTGCCGAGCG CCTGCCGTAT ACGCTGGCCA TCCATGCCGG TGGCGGCGAG AACCCGCACT GCCACCTGAT GATCTCCGAG CGGATCAATG ACGGCATCGA GCGGCCCGCC GCTCAGTGGT TCAAGCGGTA CAACGGCAAG ACCCCGGAGA AGGGCGGGGC ACAGAAGACC GAAGCGCTCA AGCCCAAGGC ATGGCTTGAG CAGACCCGCG AGGCATGGGC CGACCATGCC AACCGGGCAT TAGAGCGGGC TGGCCACGAC GCCCGCATTG ACCACAGAAC ACTTGAGGCG CAGGGCATCG AGCGCCTGCC CGGTGTTCAC CTGGGGCCGA ACGTGGTGGA GATGGAAGGC CGGGGCATCC GCACCGACCG GGCAGACGTG GCCCTGAACA TCGACACCGC CAACACCCAG ATCATCGACT TACAGGAATA CCGGGAGGCA ATAGACCATG AACGCAATCG ACAGAGTGAA GAAATCCAGA GGCATCAACG AGTTAGCGGA GCAGATCGAA CCGCTGGCCC AGAGCATGGC GACACTGGCC GACGAAGCCC GGCAGGTCAT GAGCCAGACC CAGCAGGCCA GCGAGGCGCA GGCGGCGGAG TGGCTGAAAG CCCAGCGCCA GACAGGGGCG GCATGGGTGG AGCTGGCCAA AGAGTTGCGG GAGGTAGCCG CCGAGGTGAG CAGCGCCGCG CAGAGCGCCC GGAGCGCGTC GCGGGGGTGG CACTGGAAGC TATGGCTAAC CGTGATGCTG GCTTCCATGA TGCCTACGGT GGTGCTGCTG ATCGCATCGT TGCTCTTGCT CGACCTGACG CCACTGACAA CCGAGGACGG CTCGATCTGG CTGCGCTTGG TGGCCCGATG AAGAACGACA GGACTTTGCA GGCCATAGGC CGACAGCTCA AGGCCATGGG CTGTGAGCGC TTCGATATCG GCGTCAGGGA CGCCACCACC GGCCAGATGA TGAACCGGGA ATGGTCAGCC GCCGAAGTGC TCCAGAACAC GCCATGGCTC AAGCGGATGA ATGCCCAGGG CAATGACGTG TATATCAGGC CCGCCGAGCA GGAGCGGCAT GGTCTGGTGC TGGTGGACGA CCTCAGCGAG TTTGACCTGG ATGACATGAA AGCCGAGGGC CGGGAGCCTG CCCTGGTAGT GGAAACCAGC CCGAAGAACT ATCAGGCATG GGTCAAGGTG GCCGACGCCG CAGGCGGTGA ACTTCGGGGG CAGATTGCCC GGACGCTGGC CAGCGAGTAC GACGCCGACC CGGCCAGCGC CGACAGCCGC CACTATGGCC GCTTGGCGGG CTTCACCAAC CGCAAGGACA AGCACACCAC CCGCGCCGGT TATCAGCCGT GGGTGCTGCT GCGTGAATCC AAGGGCAAGA CCGCCACCGC TGGCCCGGCG CTGGTGCAGC AGGCTGGCCA GCAGATCGAG CAGGCCCAGC GGCAGCAGGA GAAGGCCCGC AGGCTGGCCA GCCTCGAACT GCCCGAGCGG CAGCTTAGCC GCCACCGGCG CACGGCGCTG GACGAGTACC GCAGCGAGAT GGCCGGGCTG GTCAAGCGCT TCGGTGATGA CCTCAGCAAG TGCGACTTTA TCGCCGCGCA GAAGCTGGCC AGCCGGGGCC GCAGTGCCGA GGAAACCGGC AAGGCCATGG CCGAGGCCAG CCCAGCGCTG GCAGAGCGCA AGCCCGGCCA CGAAGCGGAT TACATCGAGC GCACCGTCAG CAAGGTCATG GGTCTGCCCA GCGTCCAGCT TGCGCGGGCC GAGCTGGCAC GGGCACCGGC ACCCCGCCAG CGAGGCATGG ACAGGGGCGG GCCAGATTTC AGCATGTAGT GCTTGCGTTG GTACTCACGC CTGTTATACT ATGAGTACTC ACGCACAGAA GGGGGTTTTA TGGAATACGA AAAAAGCGCT TCAGGGTCGG TCTACCTGAT CAAAAGTGAC AAGGGCTATT GGTTGCCCGG TGGCTTTGGT TATACGTCAA ACAAGGCCGA GGCTGGCCGC TTTTCAGTCG CTGATATGGC CAGCCTTAAC CTTGACGGCT GCACCTTGTC CTTGTTCCGC GAAGACAAGC CTTTCGGCCC CGGCAAGTTT CTCGGTGACT GATATGAAAG ACCAAAAGGA CAAGCAGACC GGCGACCTGC TGGCCAGCCC TGACGCTGTA CGCCAAGCGC GATATGCCGA GCGCATGAAG GCCAAAGGGA TGCGTCAGCG CAAGTTCTGG CTGACCGACG ACGAATACGA GGCGCTGCGC GAGTGCCTGG AAGAACTCAG AGCGGCGCAG GGCGGGGGTA GTGACCCCGC CAGCGCCTAA CCACCAACTG CCTGCAAAGG AGGCAATCAA TGGCTACCCA TAAGCCTATC AATATTCTGG AGGCGTTCGC AGCAGCGCCG CCACCGCTGG ACTACGTTTT GCCCAACATG GTGGCCGGTA CGGTCGGGGC GCTGGTGTCG CCCGGTGGTG CCGGTAAATC CATGCTGGCC CTGCAACTGG CCGCACAGAT TGCAGGCGGG CCGGATCTGC TGGAGGTGGG CGAACTGCCC ACCGGCCCGG TGATCTACCT GCCCGCCGAA GACCCGCCCA CCGCCATTCA TCACCGCCTG CACGCCCTTG GGGCGCACCT CAGCGCCGAG GAACGGCAAG CCGTGGCTGA CGGCCTGCTG ATCCAGCCGC TGATCGGCAG CCTGCCCAAC ATCATGGCCC CGGAGTGGTT CGACGGCCTC AAGCGCGCCG CCGAGGGCCG CCGCCTGATG GTGCTGGACA CGCTGCGCCG GTTCCACATC GAGGAAGAAA ACGCCAGCGG CCCCATGGCC CAGGTCATCG GTCGCATGGA GGCCATCGCC GCCGATACCG GGTGCTCTAT CGTGTTCCTG CACCATGCCA GCAAGGGCGC GGCCATGATG GGCGCAGGCG ACCAGCAGCA GGCCAGCCGG GGCAGCTCGG TACTGGTCGA TAACATCCGC TGGCAGTCCT ACCTGTCGAG CATGACCAGC GCCGAGGCCG AGGAATGGGG TGTGGACGAC GACCAGCGCC GGTTCTTCGT CCGCTTCGGT GTGAGCAAGG CCAACTATGG CGCACCGTTC GCTGATCGGT GGTTCAGGCG GCATGACGGC GGGGTGCTCA AGCCCGCCGT GCTGGAGAGG CAGCGCAAGA GCAAGGGGGT GCCCCGTGGT GAAGCCTAAG AACAAGCACA GCCTCAGCCA CGTCCGGCAC GACCCGGCGC ACTGTCTGGC CCCCGGCCTG TTCCGTGCCC TCAAGCGGGG CGAGCGCAAG CGCAGCAAGC TGGACGTGAC GTATGACTAC GGTGACGGCA AGCGGATCGA GTTCAGCGGC CCGGAGCCGC TGGGCGCTGA TGATCTGCGC ATCCTGCAAG GGCTGGTGGC CATGGCTGGG CCTAATGGCC TAGTGCTTGG CCCGGAACCC AAGACCGAAG GCGGACGGCA GCTCCGGCTG TTCCTGGAAC CCAAGTGGGA GGCCGTCACC GCTGATGCCA TGGTGGTCAA AGGTAGCTAT CGGGCGCTGG CAAAGGAAAT CGGGGCAGAG GTCGATAGTG GTGGGGCGCT CAAGCACATA CAGGACTGCA TCGAGCGCCT TTGGAAGGTA TCCATCATCG CCCAGAATGG CCGCAAGCGG CAGGGGTTTC GGCTGCTGTC GGAGTACGCC AGCGACGAGG CGGACGGGCG CCTGTACGTG GCCCTGAACC CCTTGATCGC GCAGGCCGTC ATGGGTGGCG GCCAGCATGT GCGCATCAGC ATGGACGAGG TGCGGGCGCT GGACAGCGAA ACCGCCCGCC TGCTGCACCA GCGGCTGTGT GGCTGGATCG ACCCCGGCAA AACCGGCAAG GCTTCCATAG ATACCTTGTG CGGCTATGTC TGGCCGTCAG AGGCCAGTGG TTCGACCATG CGCAAGCGCC GCCAGCGGGT GCGCGAGGCG TTGCCGGAGC TGGTCGCGCT GGGCTGGACG GTAACCGAGT TCGCGGCGGG CAAGTACGAC ATCACCCGGC CCAAGGCGGC AGGCTGACCC CCCCCACTCT ATTGTAAACA AGACATTTTT ATCTTTTATA TTCAATGGCT TATTTTCCTG CTAATTGGTA ATACCATGAA AAATACCATG CTCAGAAAAG GCTTAACAAT ATTTTGAAAA ATTGCCTACT GAGCGCTGCC GCACAGCTCC ATAGGCCGCT TTCCTGGCTT TGCTTCCAGA TGTATGCTCT TCTGCTCCGA TCTGCGGGCA GTGAGCGCAA CGCAATTAAT GTAAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATA ATGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACA TATGAAACCA GTAACGTTAT ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG GTGAACCAGG CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG GAGCTGAATT ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG ATTGGCGTTG CCACCTCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT AAATCTCGCG CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA ACGAAGCGGC GTCGAAGCCT GTAAAGCGGC GGTGCACAAT CTTCTCGCGC AACGCGTCAG TGGGCTGATC ATTAACTATC CGCTGGATGA CCAGGATGCC ATTGCTGTGG AAGCTGCCTG CACTAATGTT CCGGCGTTAT TTCTTGATGT CTCTGACCAG ACACCCATCA ACAGTATTAT TTTCTCCCAT GAAGACGGTA CGCGACTGGG CGTGGAGCAT CTGGTCGCAT TGGGTCACCA GCAAATCGCG CTGTTAGCGG GCCCATTAAG TTCTGTCTCG GCGCGTCTGC GTCTGGCTGG CTGGCATAAA TATCTCACTC GCAATCAAAT TCAGCCGATA GCGGAACGGG AAGGCGACTG GAGTGCCATG TCCGGTTTTC AACAAACCAT GCAAATGCTG AATGAGGGCA TCGTTCCCAC TGCGATGCTG GTTGCCAACG ATCAGATGGC GCTGGGCGCA ATGCGCGCCA TTACCGAGTC CGGGCTGCGC GTTGGTGCGG ATATCTCGGT AGTGGGATAC GACGATACCG AAGACAGCTC ATGTTATATC CCGCCGTTAA CCACCATCAA ACAGGATTTT CGCCTGCTGG GGCAAACCAG CGTGGACCGC TTGCTGCAAC TCTCTCAGGG CCAGGCGGTG AAGGGCAATC AGCTGTTGCC CGTCTCACTG GTGAAAAGAA AAACCACCCT GGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGGAT CTCGAAGCTG ATCCGCGGTG ATCGGCACGT AAGAGGTTCC AACTTTCACC ATAATGAAAT AAGATCACTA CCGGGCGTAT TTTTTGAGTT ATCGAGATTT TCAGGAGCTA AGGAAGCTAA AATGGAGAAA AAAATCACTG GATATACCAC CGTTGATATA TCCCAATGGC ATCGTAAAGA ACATTTTGAG GCATTTCAGT CAGTTGCTCA ATGTACCTAT AACCAGACCG TTCAGCTGGA TATTACGGCC TTTTTAAAGA CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGCCTTTAT TCACATTCTT GCCCGCCTGA TGAATGCTCA TCCGGAGTTC CGTATGGCAA TGAAAGACGG TGAGCTGGTG ATATGGGATA GTGTTCACCC TTGTTACACC GTTTTCCATG AGCAAACTGA AACGTTTTCA TCGCTCTGGA GTGAATACCA CGACGATTTC CGGCAGTTTC TACACATATA TTCGCAAGAT GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT TTCCCTAAAG GGTTTATTGA GAATATGTTT TTCGTCTCAG CCAATCCCTG GGTGAGTTTC ACCAGTTTTG ATTTAAACGT GGCCAATATG GACAACTTCT TCGCCCCCGT TTTCACTATG GGCAAATATT ATACGCAAGG CGACAAGGTG CTGATGCCGC TGGCGATTCA GGTTCATCAT GCCGTCTGTG ATGGCTTCCA TGTCGGCAGA ATGCTTAATG AATTACAACA GTACTGCGAT GAGTGGCAGG GCGGGGCGTA AGTCGACCTG CAGGCATGCA AGCTTAGATC CTTTGCCTGG CGGCAGTAGC GCGGTGGTCC CACCTGACCC CATGCCGAAC TCAGAAGTGA AACGCCGTAG CGCCGATGGT AGTGTGGGGT CTCCCCATGC GAGAGTAGGG AACTGCCAGG CATTAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG CAACGGCCCG GAGGGTGGCG GGCAGGACGC CCGCCATAAA CTGCCAGGCA TCAAATTAAG CAGAAGGCCA TCCTGACGGA TGGCCTTTTT GCGTTTCTAC AAACTCTTTT TGGGTACCAG ATCT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
