NM_052867.4;bA430M15.1; CanIon; CLIFAHDD; IHPRF;IHPRF1; INNFD; VGCNL1
100μg liquid plasmid(Instantly):$149
| name | pUC57-NALCN(human) | another name | apUC57-NALCN(human) |
| Insert Size (bp) | 5217bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CATGCTCAAA AGGAAGCAGA GTTCCAGGGT GGAAGCCCAG CCAGTCACTG ACTTTGGTCC TGATGAGTCT CTGTCGGATA ATGCTGACAT CCTCTGGATT AACAAACCAT GGGTTCACTC TTTGCTGCGC ATCTGTGCCA TCATCAGCGT CATTTCTGTT TGTATGAATA CGCCAATGAC CTTCGAGCAC TATCCTCCAC TTCAGTATGT GACCTTCACT TTGGATACAT TATTGATGTT TCTCTACACG GCAGAGATGA TAGCAAAAAT GCACATCCGG GGCATTGTCA AGGGGGATAG TTCCTATGTG AAAGATCGCT GGTGTGTTTT TGATGGATTT ATGGTCTTTT GCCTTTGGGT TTCTTTGGTG CTACAGGTGT TTGAAATTGC TGATATAGTT GATCAGATGT CACCTTGGGG CATGTTGCGG ATTCCACGGC CACTGATTAT GATCCGAGCA TTCCGGATTT ATTTCCGATT TGAACTGCCA AGGACCAGAA TTACAAATAT TTTAAAGCGA TCGGGAGAAC AAATATGGAG TGTTTCCATT TTTCTACTTT TCTTTCTACT TCTTTATGGA ATTTTAGGAG TTCAGATGTT TGGAACATTT ACTTATCACT GTGTTGTAAA TGACACAAAG CCAGGGAATG TAACCTGGAA TAGTTTAGCT ATTCCAGACA CACACTGCTC ACCAGAGCTA GAAGAAGGCT ACCAGTGCCC ACCTGGATTT AAATGCATGG ACCTTGAAGA TCTGGGACTT AGCAGGCAAG AGCTGGGCTA CAGTGGCTTT AATGAGATAG GAACTAGTAT ATTCACCGTC TATGAGGCCG CCTCACAGGA AGGCTGGGTG TTCCTCATGT ACAGAGCAAT TGACAGCTTT CCCCGTTGGC GTTCCTACTT CTATTTCATC ACTCTCATTT TCTTCCTCGC CTGGCTTGTG AAGAACGTGT TTATTGCTGT TATCATTGAA ACATTTGCAG AAATCAGAGT ACAGTTTCAA CAAATGTGGG GATCGAGAAG CAGCACTACC TCAACAGCCA CCACCCAGAT GTTTCATGAA GATGCTGCTG GAGGTTGGCA GCTGGTAGCT GTGGATGTCA ACAAGCCCCA GGGACGCGCC CCAGCCTGCC TCCAGAAAAT GATGCGGTCA TCCGTTTTCC ACATGTTCAT CCTGAGCATG GTGACCGTGG ACGTGATCGT GGCGGCTAGC AACTACTACA AAGGAGAAAA CTTCAGGAGG CAGTACGACG AGTTCTACCT GGCGGAGGTG GCTTTTACAG TACTTTTTGA TTTGGAAGCA CTTCTGAAGA TATGGTGTTT GGGATTTACT GGATATATTA GCTCATCTCT CCACAAATTC GAACTACTAC TCGTAATTGG AACTACTCTT CATGTATACC CAGATCTTTA TCATTCACAA TTCACGTACT TTCAGGTTCT CCGAGTAGTT CGGCTGATTA AGATTTCACC TGCATTAGAA GACTTTGTGT ACAAGATATT TGGTCCTGGA AAAAAGCTTG GGAGTTTGGT TGTATTTACT GCCAGCCTCT TGATTGTTAT GTCAGCAATT AGTTTGCAGA TGTTCTGCTT TGTCGAAGAA CTGGACAGAT TTACTACGTT TCCGAGGGCA TTTATGTCCA TGTTCCAGAT CCTCACCCAG GAAGGATGGG TGGACGTAAT GGACCAAACT CTAAATGCTG TGGGACATAT GTGGGCACCC GTGGTTGCCA TCTATTTCAT TCTCTATCAT CTTTTTGCCA CTCTGATCCT CCTGAGTTTG TTTGTTGCTG TTATTTTGGA CAACTTAGAA CTTGATGAAG ACCTAAAGAA GCTTAAACAA TTAAAGCAAA GTGAAGCAAA TGCGGACACC AAAGAAAAGC TCCCTTTACG CCTGCGAATC TTTGAAAAAT TTCCAAACAG ACCTCAAATG GTGAAAATCT CAAAGCTTCC TTCAGATTTT ACAGTTCCTA AAATCAGGGA GAGTTTTATG AAGCAGTTTA TTGACCGCCA GCAACAGGAC ACATGTTGCC TCCTGAGAAG CCTCCCGACC ACCTCTTCCT CCTCCTGCGA CCACTCCAAA CGCTCAGCAA TTGAGGACAA CAAATACATC GACCAAAAAC TTCGCAAGTC TGTTTTCAGC ATCAGGGCAA GGAACCTTCT GGAAAAGGAG ACCGCAGTCA CTAAAATCTT AAGAGCTTGC ACCCGACAGC GCATGCTGAG CGGATCATTT GAGGGGCAGC CCGCAAAGGA GAGGTCAATC CTCAGCGTGC AGCATCATAT CCGCCAAGAG CGCAGGTCAC TAAGACATGG ATCAAACAGC CAGAGGATCA GCAGGGGAAA ATCTCTTGAA ACTTTGACTC AAGATCATTC CAATACAGTG AGATATAGAA ATGCACAAAG AGAAGACAGT GAAATAAAGA TGATTCAGGA AAAAAAGGAG CAAGCAGAGA TGAAAAGGAA AGTGCAAGAA GAGGAACTCA GAGAGAACCA CCCATACTTC GATAAGCCAC TGTTCATTGT CGGGCGAGAA CACAGGTTCA GAAACTTTTG CCGGGTGGTG GTCCGAGCAC GCTTCAACGC ATCTAAAACA GACCCTGTCA CAGGAGCTGT GAAAAATACA AAGTACCATC AACTTTATGA TTTGCTGGGA TTGGTCACTT ACCTGGACTG GGTCATGATC ATCGTAACCA TCTGCTCTTG CATTTCCATG ATGTTTGAGT CCCCGTTTCG AAGAGTCATG CATGCACCTA CTTTGCAGAT TGCTGAGTAT GTGTTTGTGA TATTCATGAG CATTGAGCTT AATCTGAAGA TTATGGCAGA TGGCTTATTT TTCACTCCAA CTGCTGTCAT CAGGGACTTC GGTGGAGTAA TGGACATATT TATATATCTT GTGAGCTTGA TATTTCTTTG TTGGATGCCT CAAAATGTAC CTGCTGAATC GGGAGCTCAG CTTCTAATGG TCCTTCGGTG CCTGAGACCT CTGCGCATAT TCAAACTGGT GCCCCAGATG AGGAAAGTTG TTCGAGAACT TTTCAGCGGC TTCAAGGAAA TTTTTTTGGT CTCCATTCTT TTGCTGACAT TAATGCTCGT TTTTGCAAGC TTTGGAGTTC AGCTTTTTGC TGGAAAACTG GCCAAGTGCA ATGATCCCAA CATTATTAGA AGGGAAGATT GCAATGGCAT ATTCAGAATT AATGTCAGTG TGTCAAAGAA CTTAAATTTA AAATTGAGGC CTGGAGAGAA AAAACCTGGA TTTTGGGTGC CCCGTGTTTG GGCGAATCCT CGGAACTTTA ATTTCGACAA TGTGGGAAAC GCTATGCTGG CGTTGTTTGA AGTTCTCTCC TTGAAAGGCT GGGTGGAAGT GAGAGATGTT ATTATTCATC GTGTGGGGCC GATCCATGGA ATCTATATTC ATGTTTTTGT ATTCCTGGGT TGCATGATTG GACTGACCCT TTTTGTTGGA GTAGTTATTG CTAATTTCAA TGAAAACAAG GGGACGGCTT TGCTGACCGT CGATCAGAGA AGATGGGAAG ACCTGAAGAG CCGACTGAAG ATCGCACAGC CTCTTCATCT TCCGCCTCGC CCGGATAATG ATGGTTTTAG AGCTAAAATG TATGACATAA CCCAGCATCC ATTTTTTAAG AGGACAATCG CATTACTCGT CCTGGCCCAG TCGGTGTTGC TCTCTGTCAA GTGGGACGTC GAGGACCCGG TGACCGTACC TTTGGCAACA ATGTCAGTTG TTTTCACCTT CATCTTTGTT CTGGAGGTTA CCATGAAGAT CATAGCAATG TCGCCTGCTG GCTTCTGGCA AAGCAGAAGA AACCGATACG ATCTCCTGGT GACGTCGCTT GGCGTTGTAT GGGTGGTGCT TCACTTTGCC CTCCTGAATG CATATACTTA CATGATGGGC GCTTGTGTGA TTGTATTTAG GTTTTTCTCC ATCTGTGGAA AACATGTAAC GCTAAAGATG CTCCTCTTGA CAGTGGTCGT CAGCATGTAC AAGAGCTTCT TTATCATAGT AGGCATGTTT CTCTTGCTGC TGTGTTACGC TTTTGCTGGA GTTGTTTTAT TTGGTACTGT GAAATATGGG GAGAATATTA ACAGGCATGC AAATTTTTCT TCGGCTGGAA AAGCTATTAC CGTACTGTTC CGAATTGTCA CAGGTGAAGA CTGGAACAAG ATTATGCATG ACTGTATGGT TCAGCCTCCG TTTTGTACTC CAGATGAATT TACATACTGG GCAACAGACT GTGGAAATTA TGCTGGGGCA CTTATGTATT TCTGTTCATT TTATGTCATC ATTGCCTACA TCATGCTAAA TCTGCTTGTA GCCATAATTG TGGAGAATTT CTCCTTGTTT TATTCCACTG AGGAGGACCA GCTTTTAAGT TACAATGATC TTCGCCACTT TCAAATAATA TGGAACATGG TGGATGATAA AAGAGAGGGG GTGATCCCCA CGTTCCGCGT CAAGTTCCTG CTGCGGCTAC TGCGTGGGAG GCTGGAGGTG GACCTGGACA AGGACAAGCT CCTGTTTAAG CACATGTGCT ACGAAATGGA GAGGCTCCAC AATGGCGGCG ACGTCACCTT CCATGATGTC CTGAGCATGC TTTCATACCG GTCCGTGGAC ATCCGGAAGA GCTTGCAGCT GGAGGAACTC CTGGCGAGGG AGCAGCTGGA GTACACCATA GAGGAGGAGG TGGCCAAGCA GACCATCCGC ATGTGGCTCA AGAAGTGCCT GAAGCGCATC AGAGCTAAAC AGCAGCAGTC GTGCAGTATC ATCCACAGCC TGAGAGAGAG TCAGCAGCAA GAGCTGAGCC GGTTTCTGAA CCCGCCCAGC ATCGAGACCA CCCAGCCCAG TGAGGACACG AATGCCAACA GTCAGGACAA CAGCATGCAA CCTGAGACAA GCAGCCAGCA GCAGCTCCTG AGCCCCACGC TGTCGGATCG AGGAGGAAGT CGGCAAGATG CAGCCGACGC AGGGAAACCC CAGAGGAAAT TTGGGCAGTG GCGTCTGCCC TCAGCCCCAA AACCAATAAG CCATTCAGTG TCCTCAGTCA ACTTACGGTT TGGAGGAAGG ACAACCATGA AATCTGTCGT GTGCAAAATG AACCCCATGA CTGACGCGGC TTCCTGCGGT TCTGAAGTTA AGAAGTGGTG GACCCGGCAG CTGACTGTGG AGAGCGACGA AAGTGGGGAT GACCTTCTGG ATATTTAGAA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTC
