NM_007192.4;CDC68; FACTP140; NEDDFAC; SPT16;PT16/CDC68
100μg liquid plasmid(Instantly):$142
| name | pEF1a-SUPT16H(human)-FLAG-Neo | another name | apEF1a-SUPT16H(human)-FLAG-Neo |
| Insert Size (bp) | 3144bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | EF1a | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGCGTTGACA TTGATTATTG ACTAGGCTTT TGCAAAAAGC TTTGCAAAGA TGGATAAAGT TTTAAACAGA GAGGAATCTT TGCAGCTAAT GGACCTTCTA GGTCTTGAAA GGAGTGGGAA TTGGCTCCGG TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG CCGTGTGTGG TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA TTACTTCCAC CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT GGGAGAGTTC GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT GGCCTGGGCG CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG CTTTCGATAA GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT GGCAAGATAG TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG CCGCGGGCGG CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC GAGCGCGGCC ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC CAGTTGCGTG AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA GGACGCGGCG CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC CGTCCTCAGC CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG ATTAGTTCTC GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA TGGAGTTTCC CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT AATTCTCCTT GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTAGC TTGGTACGCT AGCGTTTAAA CTTAAGCTTG GTACCGCCAC CATGGCTGTG ACTCTGGACA AAGACGCTTA TTATCGGCGA GTGAAGAGAC TGTACAGCAA TTGGCGGAAA GGAGAAGATG AGTATGCCAA CGTTGATGCC ATTGTTGTAT CAGTGGGTGT TGATGAAGAA ATTGTTTATG CCAAATCAAC TGCCTTACAG ACATGGCTCT TTGGTTATGA ACTAACTGAT ACTATCATGG TCTTTTGTGA TGACAAAATC ATCTTTATGG CCAGCAAGAA AAAAGTGGAG TTCTTGAAAC AGATTGCCAA CACTAAGGGC AATGAGAATG CTAATGGAGC CCCTGCCATC ACACTGCTAA TACGAGAAAA GAATGAAAGT AATAAGAGTA GCTTTGACAA AATGATTGAA GCCATTAAAG AAAGCAAGAA TGGCAAGAAG ATTGGAGTGT TCAGCAAAGA CAAATTCCCT GGAGAGTTCA TGAAGAGCTG GAATGACTGC CTCAACAAAG AAGGCTTTGA CAAAATAGAT ATCAGTGCAG TTGTGGCATA TACCATCGCT GTAAAGGAGG ATGGGGAGCT CAACCTAATG AAGAAAGCAG CCAGCATCAC TTCTGAAGTC TTCAACAAAT TCTTCAAGGA AAGAGTCATG GAAATAGTTG ATGCAGATGA GAAAGTTCGA CACAGCAAAC TGGCTGAGTC TGTGGAAAAG GCCATTGAAG AGAAAAAATA CCTTGCTGGG GCAGACCCTT CTACTGTGGA AATGTGTTAC CCTCCTATCA TTCAGAGTGG TGGCAACTAT AATCTCAAGT TCAGTGTGGT GAGTGACAAG AATCATATGC ACTTTGGGGC TATCACTTGT GCCATGGGTA TTCGCTTCAA GTCTTACTGC TCCAACCTTG TTCGCACTTT GATGGTTGAT CCTTCTCAAG AAGTTCAAGA AAATTATAAC TTTTTGCTCC AGCTTCAAGA GGAGCTGCTG AAGGAATTAA GACATGGTGT GAAGATATGT GACGTGTATA ACGCTGTCAT GGACGTGGTT AAAAAGCAGA AGCCAGAACT GCTGAACAAA ATTACCAAAA ACCTAGGGTT TGGGATGGGA ATTGAATTCC GTGAAGGCTC CCTAGTAATC AATAGCAAAA ATCAATACAA ACTGAAGAAA GGAATGGTTT TCAGCATCAA TTTAGGATTC TCAGACCTGA CTAACAAGGA GGGGAAAAAG CCAGAAGAGA AAACCTATGC CCTGTTCATT GGTGACACAG TGCTTGTGGA TGAGGATGGC CCAGCTACTG TTCTCACTTC TGTGAAGAAG AAAGTGAAGA ATGTGGGGAT TTTCCTAAAG AATGAAGATG AGGAAGAAGA GGAGGAGGAG AAAGATGAGG CAGAGGACCT TTTGGGAAGA GGTTCTCGGG CAGCATTACT TACAGAAAGA ACAAGAAATG AAATGACTGC AGAAGAGAAG CGAAGAGCAC ATCAGAAAGA ACTAGCGGCT CAACTCAATG AAGAAGCAAA GAGGCGATTG ACTGAACAAA AGGGAGAACA GCAGATTCAG AAAGCTCGCA AGTCTAATGT GTCCTATAAA AACCCATCTC TGATGCCTAA GGAACCACAT ATTCGGGAAA TGAAGATCTA CATCGATAAG AAATATGAGA CTGTAATAAT GCCCGTGTTT GGCATTGCAA CACCGTTTCA CATTGCCACA ATCAAGAATA TAAGTATGTC CGTGGAAGGA GATTATACTT ACTTGCGAAT CAACTTTTAT TGCCCAGGCA GTGCTCTGGG CAGGAATGAA GGCAACATCT TTCCTAACCC TGAAGCGACT TTTGTCAAGG AAATTACATA CCGAGCATCA AATATTAAGG CACCCGGAGA ACAGACAGTA CCAGCCTTGA ACCTTCAGAA TGCTTTCCGA ATTATTAAAG AAGTACAGAA ACGTTATAAA ACTCGAGAAG CTGAAGAGAA AGAGAAGGAG GGGATTGTAA AACAAGACTC ACTGGTGATC AATCTAAACC GGAGTAATCC GAAACTGAAA GATCTATACA TTCGCCCAAA TATTGCCCAA AAGAGGATGC AAGGCTCACT GGAGGCCCAT GTCAATGGCT TCCGCTTCAC ATCTGTTCGA GGAGACAAAG TGGATATTTT GTACAATAAT ATTAAGCATG CTTTGTTCCA GCCCTGTGAT GGAGAAATGA TTATTGTCTT GCACTTTCAC CTCAAGAATG CCATCATGTT TGGGAAGAAG CGGCACACGG ATGTGCAGTT CTACACAGAA GTGGGAGAGA TAACCACGGA CTTGGGGAAA CATCAGCATA TGCATGACCG AGATGACCTC TATGCTGAGC AGATGGAACG AGAAATGAGG CACAAACTGA AAACAGCCTT TAAAAATTTC ATTGAGAAAG TAGAGGCTCT AACTAAGGAG GAACTGGAAT TTGAAGTGCC TTTTAGGGAC TTGGGATTTA ACGGAGCTCC CTATAGGAGT ACCTGCCTCC TTCAGCCCAC TAGTAGTGCG CTGGTAAATG CTACGGAATG GCCACCTTTT GTGGTGACAT TGGATGAGGT AGAGCTGATC CACTTTGAGC GGGTCCAGTT TCACCTGAAG AACTTTGATA TGGTAATCGT CTACAAGGAC TACAGCAAGA AAGTGACCAT GATCAACGCC ATTCCTGTAG CCTCTCTTGA CCCCATCAAG GAATGGTTGA ATTCCTGCGA CCTGAAATAC ACAGAAGGAG TACAGTCCCT CAACTGGACT AAAATCATGA AGACCATTGT TGATGACCCT GAGGGCTTCT TCGAACAAGG TGGCTGGTCT TTCCTGGAGC CTGAGGGTGA GGGGAGTGAT GCTGAAGAAG GGGATTCAGA GTCTGAAATT GAAGATGAGA CTTTTAATCC TTCAGAAGAT GACTATGAAG AGGAAGAGGA GGACAGTGAT GAAGATTATT CATCAGAAGC AGAAGAGTCA GACTATTCTA AGGAGTCATT GGGTAGTGAA GAAGAGAGTG GAAAGGATTG GGATGAACTG GAGGAAGAAG CCCGAAAAGC GGACCGAGAA AGTCGTTACG AGGAAGAAGA AGAACAAAGT CGAAGTATGA GCCGGAAGAG GAAGGCATCT GTGCACAGTT CGGGCCGTGG CTCTAACCGT GGTTCCAGAC ACAGCTCTGC ACCCCCCAAG AAAAAGAGGA AGGTCGACGA CTACAAGGAC GACGATGACA AGTAGCGGCC GCGACTCTAG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTAAGG CGTAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT CCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAG ATCGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC TAGGGGGAGG CTAACTGAAA CACGGAAGGA GACAATACCG GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCCTCAGGT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGT
