pMDC43
$113.00
In stock
SKU
PL41459
100μg liquid plasmid(Instantly):$134
| name | pMDC43 | another name | apMDC43 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF,GateWay |
| Fragment species | Empty vector | Prokaryotic resistance | Kan,Chl |
| Selective marker | Hyg | Fluorescent labeling | Green |
| Promoter | 35s | Replicon | pVS1 |
| Competence | DB3.1 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AATTCAGTAA CATAGATGAC ACCGCGCGCG ATAATTTATC CTAGTTTGCG CGCTATATTT TGTTTTCTAT CGCGTATTAA ATGTATAATT GCGGGACTCT AATCATAAAA ACCCATCTCA TAAATAACGT CATGCATTAC ATGTTAATTA TTACATGCTT AACGTAATTC AACAGAAATT ATATGATAAT CATCGCAAGA CCGGCAACAG GATTCAATCT TAAGAAACTT TATTGCCAAA TGTTTGAACG ATCGGGGAAA TTCGAGCTCC ACCGCGGTGG CGGCCGCTCT AGAACTAGTT AATTAAGAAT TATCGAACCA CTTTGTACAA GAAAGCTGAA CGAGAAACGT AAAATGATAT AAATATCAAT ATATTAAATT AGATTTTGCA TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC CAGTCACTAT GGTCGACCTG CAGACTGGCT GTGTATAAGG GAGCCTGACA TTTATATTCC CCAGAACATC AGGTTAATGG CGTTTTTGAT GTCATTTTCG CGGTGGCTGA GATCAGCCAC TTCTTCCCCG ATAACGGAGA CCGGCACACT GGCCATATCG GTGGTCATCA TGCGCCAGCT TTCATCCCCG ATATGCACCA CCGGGTAAAG TTCACGGGAG ACTTTATCTG ACAGCAGACG TGCACTGGCC AGGGGGATCA CCATCCGTCG CCCGGGCGTG TCAATAATAT CACTCTGTAC ATCCACAAAC AGACGATAAC GGCTCTCTCT TTTATAGGTG TAAACCTTAA ACTGCATTTC ACCAGTCCCT GTTCTCGTCA GCAAAAGAGC CGTTCATTTC AATAAACCGG GCGACCTCAG CCATCCCTTC CTGATTTTCC GCTTTCCAGC GTTCGGCACG CAGACGACGG GCTTCATTCT GCATGGTTGT GCTTACCAGA CCGGAGATAT TGACATCATA TATGCCTTGA GCAACTGATA GCTGTCGCTG TCAACTGTCA CTGTAATACG CTGCTTCATA GCACACCTCT TTTTGACATA CTTCGGGTAT ACATATCAGT ATATATTCTT ATACCGCAAA AATCAGCGCG CAAATACGCA TACTGTTATC TGGCTTTTAG TAAGCCGGAT CCTCTAGATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAGACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGG AATTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATTT TAGCTTCCTT AGCTCCTGAA AATCTCGCCG GATCCTAACT CAAAATCCAC ACATTATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGC GGCCGCCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC AGCTTTTTTG TACAAACTTG TTTGATAGCT TGGCGCGCCT TTGTATAGTT CATCCATGCC ATGTGTAATC CCAGCAGCTG TTACAAACTC AAGAAGGACC ATGTGGTCTC TCTTTTCGTT GGGATCTTTC GAAAGGGCAG ATTGTGTGGA CAGGTAATGG TTGTCTGGTA AAAGGACAGG GCCATCGCCA ATTGGAGTAT TTTGTTGATA ATGATCAGCG AGTTGCACGC CGCCGTCTTC GATGTTGTGG CGGGTCTTGA AGTTGGCTTT GATGCCGTTC TTTTGCTTGT CGGCCATGAT GTATACGTTG TGGGAGTTGT AGTTGTATTC CAACTTGTGG CCGAGGATGT TTCCGTCCTC CTTGAAATCG ATTCCCTTAA GCTCGATCCT GTTGACGAGG GTGTCTCCCT CAAACTTGAC TTCAGCACGT GTCTTGTAGT TCCCGTCGTC CTTGAAGAAG ATGGTCCTCT CCTGCACGTA TCCCTCAGGC ATGGCGCTCT TGAAGAAGTC GTGCCGCTTC ATATGATCAG GGTAACGGGA GAAGCACTGC ACGCCGTAGG TCAGGGTGGT GACCAGGGTT GGCCATGGAA CAGGTAGTTT TCCAGTAGTG CAAATAAATT TAAGGGTAAG TTTTCCGTAT GTTGCATCAC CTTCACCCTC TCCACTGACA GAAAATTTGT GCCCATTAAC ATCACCATCT AATTCAACAA GAATTGGGAC AACTCCAGTG AAAAGTTCTT CTCCTTTACT CATTTTTTCT ACCGGTACCC GGGGATCCTC TAGAGTCGAG GTCCTCTCCA AATGAAATGA ACTTCCTTAT ATAGAGGAAG GGTCTTGCGA AGGATAGTGG GATTGTGCGT CATCCCTTAC GTCAGTGGAG ATATCACATC AATCCACTTG CTTTGAAGAC GTGGTTGGAA CGTCTTCTTT TTCCACGATG CTCCTCGTGG GTGGGGGTCC ATCTTTGGGA CCACTGTCGG CAGAGGCATC TTCAACGATG GCCTTTCCTT TATCGCAATG ATGGCATTTG TAGGAGCCAC CTTCCTTTTC CACTATCTTC ACAATAAAGT GACAGATAGC TGGGCAATGG AATCCGAGGA GGTTTCCGGA TATTACCCTT TGTTGAAAAG TCTCAATTGC CCTTTGGTCT TCTGAGACTG TATCTTTGAT ATTTTTGGAG TAGACAAGTG TGTCGTGCTC CACCATGTTA TCACATCAAT CCACTTGCTT TGAAGACGTG GTTGGAACGT CTTCTTTTTC CACGATGCTC CTCGTGGGTG GGGGTCCATC TTTGGGACCA CTGTCGGCAG AGGCATCTTC AACGATGGCC TTTCCTTTAT CGCAATGATG GCATTTGTAG GAGCCACCTT CCTTTTCCAC TATCTTCACA ATAAAGTGAC AGATAGCTGG GCAATGGAAT CCGAGGAGGT TTCCGGATAT TACCCTTTGT TGAAAAGTCT CAATTGCCCT TTGGTCTTCT GAGACTGTAT CTTTGATATT TTTGGAGTAG ACAAGTGTGT CGTGCTCCAC CATGTTGACC TGCAGGCACG CCAAGCTTGG CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGCTA GAGCAGCTTG AGCTTGGATC AGATTGTCGT TTCCCGCCTT CAGTTTAAAC TATCAGTGTT TGACAGGATA TATTGGCGGG TAAACCTAAG AGAAAAGAGC GTTTATTAGA ATAACGGATA TTTAAAAGGG CGTGAAAAGG TTTATCCGTT CGTCCATTTG TATGTGCATG CCAACCACAG GGTTCCCCTC GGGATCAAAG TACTTTGATC CAACCCCTCC GCTGCTATAG TGCAGTCGGC TTCTGACGTT CAGTGCAGCC GTCTTCTGAA AACGACATGT CGCACAAGTC CTAAGTTACG CGACAGGCTG CCGCCCTGCC CTTTTCCTGG CGTTTTCTTG TCGCGTGTTT TAGTCGCATA AAGTAGAATA CTTGCGACTA GAACCGGAGA CATTACGCCA TGAACAAGAG CGCCGCCGCT GGCCTGCTGG GCTATGCCCG CGTCAGCACC GACGACCAGG ACTTGACCAA CCAACGGGCC GAACTGCACG CGGCCGGCTG CACCAAGCTG TTTTCCGAGA AGATCACCGG CACCAGGCGC GACCGCCCGG AGCTGGCCAG GATGCTTGAC CACCTACGCC CTGGCGACGT TGTGACAGTG ACCAGGCTAG ACCGCCTGGC CCGCAGCACC CGCGACCTAC TGGACATTGC CGAGCGCATC CAGGAGGCCG GCGCGGGCCT GCGTAGCCTG GCAGAGCCGT GGGCCGACAC CACCACGCCG GCCGGCCGCA TGGTGTTGAC CGTGTTCGCC GGCATTGCCG AGTTCGAGCG TTCCCTAATC ATCGACCGCA CCCGGAGCGG GCGCGAGGCC GCCAAGGCCC GAGGCGTGAA GTTTGGCCCC CGCCCTACCC TCACCCCGGC ACAGATCGCG CACGCCCGCG AGCTGATCGA CCAGGAAGGC CGCACCGTGA AAGAGGCGGC TGCACTGCTT GGCGTGCATC GCTCGACCCT GTACCGCGCA CTTGAGCGCA GCGAGGAAGT GACGCCCACC GAGGCCAGGC GGCGCGGTGC CTTCCGTGAG GACGCATTGA CCGAGGCCGA CGCCCTGGCG GCCGCCGAGA ATGAACGCCA AGAGGAACAA GCATGAAACC GCACCAGGAC GGCCAGGACG AACCGTTTTT CATTACCGAA GAGATCGAGG CGGAGATGAT CGCGGCCGGG TACGTGTTCG AGCCGCCCGC GCACGTCTCA ACCGTGCGGC TGCATGAAAT CCTGGCCGGT TTGTCTGATG CCAAGCTGGC GGCCTGGCCG GCCAGCTTGG CCGCTGAAGA AACCGAGCGC CGCCGTCTAA AAAGGTGATG TGTATTTGAG TAAAACAGCT TGCGTCATGC GGTCGCTGCG TATATGATGC GATGAGTAAA TAAACAAATA CGCAAGGGGA ACGCATGAAG GTTATCGCTG TACTTAACCA GAAAGGCGGG TCAGGCAAGA CGACCATCGC AACCCATCTA GCCCGCGCCC TGCAACTCGC CGGGGCCGAT GTTCTGTTAG TCGATTCCGA TCCCCAGGGC AGTGCCCGCG ATTGGGCGGC CGTGCGGGAA GATCAACCGC TAACCGTTGT CGGCATCGAC CGCCCGACGA TTGACCGCGA CGTGAAGGCC ATCGGCCGGC GCGACTTCGT AGTGATCGAC GGAGCGCCCC AGGCGGCGGA CTTGGCTGTG TCCGCGATCA AGGCAGCCGA CTTCGTGCTG ATTCCGGTGC AGCCAAGCCC TTACGACATA TGGGCCACCG CCGACCTGGT GGAGCTGGTT AAGCAGCGCA TTGAGGTCAC GGATGGAAGG CTACAAGCGG CCTTTGTCGT GTCGCGGGCG ATCAAAGGCA CGCGCATCGG CGGTGAGGTT GCCGAGGCGC TGGCCGGGTA CGAGCTGCCC ATTCTTGAGT CCCGTATCAC GCAGCGCGTG AGCTACCCAG GCACTGCCGC CGCCGGCACA ACCGTTCTTG AATCAGAACC CGAGGGCGAC GCTGCCCGCG AGGTCCAGGC GCTGGCCGCT GAAATTAAAT CAAAACTCAT TTGAGTTAAT GAGGTAAAGA GAAAATGAGC AAAAGCACAA ACACGCTAAG TGCCGGCCGT CCGAGCGCAC GCAGCAGCAA GGCTGCAACG TTGGCCAGCC TGGCAGACAC GCCAGCCATG AAGCGGGTCA ACTTTCAGTT GCCGGCGGAG GATCACACCA AGCTGAAGAT GTACGCGGTA CGCCAAGGCA AGACCATTAC CGAGCTGCTA TCTGAATACA TCGCGCAGCT ACCAGAGTAA ATGAGCAAAT GAATAAATGA GTAGATGAAT TTTAGCGGCT AAAGGAGGCG GCATGGAAAA TCAAGAACAA CCAGGCACCG ACGCCGTGGA ATGCCCCATG TGTGGAGGAA CGGGCGGTTG GCCAGGCGTA AGCGGCTGGG TTGTCTGCCG GCCCTGCAAT GGCACTGGAA CCCCCAAGCC CGAGGAATCG GCGTGACGGT CGCAAACCAT CCGGCCCGGT ACAAATCGGC GCGGCGCTGG GTGATGACCT GGTGGAGAAG TTGAAGGCCG CGCAGGCCGC CCAGCGGCAA CGCATCGAGG CAGAAGCACG CCCCGGTGAA TCGTGGCAAG CGGCCGCTGA TCGAATCCGC AAAGAATCCC GGCAACCGCC GGCAGCCGGT GCGCCGTCGA TTAGGAAGCC GCCCAAGGGC GACGAGCAAC CAGATTTTTT CGTTCCGATG CTCTATGACG TGGGCACCCG CGATAGTCGC AGCATCATGG ACGTGGCCGT TTTCCGTCTG TCGAAGCGTG ACCGACGAGC TGGCGAGGTG ATCCGCTACG AGCTTCCAGA CGGGCACGTA GAGGTTTCCG CAGGGCCGGC CGGCATGGCC AGTGTGTGGG ATTACGACCT GGTACTGATG GCGGTTTCCC ATCTAACCGA ATCCATGAAC CGATACCGGG AAGGGAAGGG AGACAAGCCC GGCCGCGTGT TCCGTCCACA CGTTGCGGAC GTACTCAAGT TCTGCCGGCG AGCCGATGGC GGAAAGCAGA AAGACGACCT GGTAGAAACC TGCATTCGGT TAAACACCAC GCACGTTGCC ATGCAGCGTA CGAAGAAGGC CAAGAACGGC CGCCTGGTGA CGGTATCCGA GGGTGAAGCC TTGATTAGCC GCTACAAGAT CGTAAAGAGC GAAACCGGGC GGCCGGAGTA CATCGAGATC GAGCTAGCTG ATTGGATGTA CCGCGAGATC ACAGAAGGCA AGAACCCGGA CGTGCTGACG GTTCACCCCG ATTACTTTTT GATCGATCCC GGCATCGGCC GTTTTCTCTA CCGCCTGGCA CGCCGCGCCG CAGGCAAGGC AGAAGCCAGA TGGTTGTTCA AGACGATCTA CGAACGCAGT GGCAGCGCCG GAGAGTTCAA GAAGTTCTGT TTCACCGTGC GCAAGCTGAT CGGGTCAAAT GACCTGCCGG AGTACGATTT GAAGGAGGAG GCGGGGCAGG CTGGCCCGAT CCTAGTCATG CGCTACCGCA ACCTGATCGA GGGCGAAGCA TCCGCCGGTT CCTAATGTAC GGAGCAGATG CTAGGGCAAA TTGCCCTAGC AGGGGAAAAA GGTCGAAAAG GTCTCTTTCC TGTGGATAGC ACGTACATTG GGAACCCAAA GCCGTACATT GGGAACCGGA ACCCGTACAT TGGGAACCCA AAGCCGTACA TTGGGAACCG GTCACACATG TAAGTGACTG ATATAAAAGA GAAAAAAGGC GATTTTTCCG CCTAAAACTC TTTAAAACTT ATTAAAACTC TTAAAACCCG CCTGGCCTGT GCATAACTGT CTGGCCAGCG CACAGCCGAA GAGCTGCAAA AAGCGCCTAC CCTTCGGTCG CTGCGCTCCC TACGCCCCGC CGCTTCGCGT CGGCCTATCG CGGCCGCTGG CCGCTCAAAA ATGGCTGGCC TACGGCCAGG CAATCTACCA GGGCGCGGAC AAGCCGCGCC GTCGCCACTC GACCGCCGGC GCCCACATCA AGGCACCCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT AGCGATAGCG GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGCA TTCTAGGTAC TAAAACAATT CATCCAGTAA AATATAATAT TTTATTTTCT CCCAATCAGG CTTGATCCCC AGTAAGTCAA AAAATAGCTC GACATACTGT TCTTCCCCGA TATCCTCCCT GATCGACCGG ACGCAGAAGG CAATGTCATA CCACTTGTCC GCCCTGCCGC TTCTCCCAAG ATCAATAAAG CCACTTACTT TGCCATCTTT CACAAAGATG TTGCTGTCTC CCAGGTCGCC GTGGGAAAAG ACAAGTTCCT CTTCGGGCTT TTCCGTCTTT AAAAAATCAT ACAGCTCGCG CGGATCTTTA AATGGAGTGT CTTCTTCCCA GTTTTCGCAA TCCACATCGG CCAGATCGTT ATTCAGTAAG TAATCCAATT CGGCTAAGCG GCTGTCTAAG CTATTCGTAT AGGGACAATC CGATATGTCG ATGGAGTGAA AGAGCCTGAT GCACTCCGCA TACAGCTCGA TAATCTTTTC AGGGCTTTGT TCATCTTCAT ACTCTTCCGA GCAAAGGACG CCATCGGCCT CACTCATGAG CAGATTGCTC CAGCCATCAT GCCGTTCAAA GTGCAGGACC TTTGGAACAG GCAGCTTTCC TTCCAGCCAT AGCATCATGT CCTTTTCCCG TTCCACATCA TAGGTGGTCC CTTTATACCG GCTGTCCGTC ATTTTTAAAT ATAGGTTTTC ATTTTCTCCC ACCAGCTTAT ATACCTTAGC AGGAGACATT CCTTCCGTAT CTTTTACGCA GCGGTATTTT TCGATCAGTT TTTTCAATTC CGGTGATATT CTCATTTTAG CCATTTATTA TTTCCTTCCT CTTTTCTACA GTATTTAAAG ATACCCCAAG AAGCTAATTA TAACAAGACG AACTCCAATT CACTGTTCCT TGCATTCTAA AACCTTAAAT ACCAGAAAAC AGCTTTTTCA AAGTTGTTTT CAAAGTTGGC GTATAACATA GTATCGACGG AGCCGATTTT GAAACCGCGG TGATCACAGG CAGCAACGCT CTGTCATCGT TACAATCAAC ATGCTACCCT CCGCGAGATC ATCCGTGTTT CAAACCCGGC AGCTTAGTTG CCGTTCTTCC GAATAGCATC GGTAACATGA GCAAAGTCTG CCGCCTTACA ACGGCTCTCC CGCTGACGCC GTCCCGGACT GATGGGCTGC CTGTATCGAG TGGTGATTTT GTGCCGAGCT GCCGGTCGGG GAGCTGTTGG CTGGCTGGTG GCAGGATATA TTGTGGTGTA AACAAATTGA CGCTTAGACA ACTTAATAAC ACATTGCGGA CGTTTTTAAT GTACTGAATT AACGCCGAAT TAATTCGGGG GATCTGGATT TTAGTACTGG ATTTTGGTTT TAGGAATTAG AAATTTTATT GATAGAAGTA TTTTACAAAT ACAAATACAT ACTAAGGGTT TCTTATATGC TCAACACATG AGCGAAACCC TATAGGAACC CTAATTCCCT TATCTGGGAA CTACTCACAC ATTATTATGG AGAAACTCGA GCTTGTCGAT CGACAGATCC GGTCGGCATC TACTCTATTT CTTTGCCCTC GGACGAGTGC TGGGGCGTCG GTTTCCACTA TCGGCGAGTA CTTCTACACA GCCATCGGTC CAGACGGCCG CGCTTCTGCG GGCGATTTGT GTACGCCCGA CAGTCCCGGC TCCGGATCGG ACGATTGCGT CGCATCGACC CTGCGCCCAA GCTGCATCAT CGAAATTGCC GTCAACCAAG CTCTGATAGA GTTGGTCAAG ACCAATGCGG AGCATATACG CCCGGAGTCG TGGCGATCCT GCAAGCTCCG GATGCCTCCG CTCGAAGTAG CGCGTCTGCT GCTCCATACA AGCCAACCAC GGCCTCCAGA AGAAGATGTT GGCGACCTCG TATTGGGAAT CCCCGAACAT CGCCTCGCTC CAGTCAATGA CCGCTGTTAT GCGGCCATTG TCCGTCAGGA CATTGTTGGA GCCGAAATCC GCGTGCACGA GGTGCCGGAC TTCGGGGCAG TCCTCGGCCC AAAGCATCAG CTCATCGAGA GCCTGCGCGA CGGACGCACT GACGGTGTCG TCCATCACAG TTTGCCAGTG ATACACATGG GGATCAGCAA TCGCGCATAT GAAATCACGC CATGTAGTGT ATTGACCGAT TCCTTGCGGT CCGAATGGGC CGAACCCGCT CGTCTGGCTA AGATCGGCCG CAGCGATCGC ATCCATAGCC TCCGCGACCG GTTGTAGAAC AGCGGGCAGT TCGGTTTCAG GCAGGTCTTG CAACGTGACA CCCTGTGCAC GGCGGGAGAT GCAATAGGTC AGGCTCTCGC TAAACTCCCC AATGTCAAGC ACTTCCGGAA TCGGGAGCGC GGCCGATGCA AAGTGCCGAT AAACATAACG ATCTTTGTAG AAACCATCGG CGCAGCTATT TACCCGCAGG ACATATCCAC GCCCTCCTAC ATCGAAGCTG AAAGCACGAG ATTCTTCGCC CTCCGAGAGC TGCATCAGGT CGGAGACGCT GTCGAACTTT TCGATCAGAA ACTTCTCGAC AGACGTCGCG GTGAGTTCAG GCTTTTTCAT ATCTCATTGC CCCCCGGGAT CTGCGAAAGC TCGAGAGAGA TAGATTTGTA GAGAGAGACT GGTGATTTCA GCGTGTCCTC TCCAAATGAA ATGAACTTCC TTATATAGAG GAAGGTCTTG CGAAGGATAG TGGGATTGTG CGTCATCCCT TACGTCAGTG GAGATATCAC ATCAATCCAC TTGCTTTGAA GACGTGGTTG GAACGTCTTC TTTTTCCACG ATGCTCCTCG TGGGTGGGGG TCCATCTTTG GGACCACTGT CGGCAGAGGC ATCTTGAACG ATAGCCTTTC CTTTATCGCA ATGATGGCAT TTGTAGGTGC CACCTTCCTT TTCTACTGTC CTTTTGATGA AGTGACAGAT AGCTGGGCAA TGGAATCCGA GGAGGTTTCC CGATATTACC CTTTGTTGAA AAGTCTCAAT AGCCCTTTGG TCTTCTGAGA CTGTATCTTT GATATTCTTG GAGTAGACGA GAGTGTCGTG CTCCACCATG TTATCACATC AATCCACTTG CTTTGAAGAC GTGGTTGGAA CGTCTTCTTT TTCCACGATG CTCCTCGTGG GTGGGGGTCC ATCTTTGGGA CCACTGTCGG CAGAGGCATC TTGAACGATA GCCTTTCCTT TATCGCAATG ATGGCATTTG TAGGTGCCAC CTTCCTTTTC TACTGTCCTT TTGATGAAGT GACAGATAGC TGGGCAATGG AATCCGAGGA GGTTTCCCGA TATTACCCTT TGTTGAAAAG TCTCAATAGC CCTTTGGTCT TCTGAGACTG TATCTTTGAT ATTCTTGGAG TAGACGAGAG TGTCGTGCTC CACCATGTTG GCAAGCTGCT CTAGCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG
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