NM_001003817.1;c-erbB2; c-neu; Erbb-2; HER-2; HER2;l11Jus8; mKIAA3023; Neu
100μg liquid plasmid(Instantly):$120
| name | pCMV-Erbb2(mouse)-3×FLAG-Puro | another name | apCMV-Erbb2(mouse)-3×FLAG-Puro |
| Insert Size (bp) | 3771bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC AAGCTTTCTA GAGAATTCGA TATCGGATCC GCCACCATGG AGCTGGCGGC CTGGTGCCGT TGGGGGTTCC TCCTCGCCCT CCTGTCCCCC GGAGCCGCGG GTACCCAAGT GTGTACCGGT ACCGACATGA AGTTGCGACT CCCTGCCAGT CCTGAGACCC ACCTGGACAT GCTTCGCCAC CTCTACCAGG GCTGTCAGGT GGTGCAGGGC AATTTGGAGC TTACCTACCT GCCCGCCAAT GCCAGCCTCT CATTCCTGCA GGACATCCAG GAAGTCCAGG GATACATGCT CATCGCTCAC AACCGAGTGA AACACGTCCC ACTGCAGAGG TTGCGCATCG TGAGAGGGAC TCAGCTCTTT GAGGACAAGT ATGCCCTGGC TGTGCTAGAC AACCGAGACC CTTTGGACAA CGTCACCACC GCCGCCCCAG GCAGAACCCC AGAAGGGCTG CGGGAGCTGC AGCTTCGAAG TCTCACAGAG ATCTTGAAGG GAGGAGTTTT GATCCGTGGG AACCCTCAGC TCTGCTACCA GGACATGGTT TTGTGGAAGG ATGTCCTCCG TAAGAATAAC CAGCTGGCTC CTGTCGACAT GGACACCAAT CGTTCCCGGG CCTGTCCACC TTGTGCCCCA ACCTGCAAAG ACAATCACTG TTGGGGTGAG AGTCCTGAAG ACTGTCAGAT CTTGACTGGC ACCATCTGTA CTAGTGGCTG TGCCCGGTGC AAGGGCCGGC TGCCCACTGA CTGTTGCCAT GAGCAGTGTG CTGCAGGCTG CACGGGTCCC AAGCATTCTG ACTGCCTGGC CTGCCTCCAC TTCAATCATA GTGGTATCTG TGAGCTGCAC TGCCCGGCCC TCATCACCTA CAACACAGAC ACCTTCGAGT CCATGCTCAA CCCTGAGGGT CGCTACACCT TTGGTGCCAG CTGTGTGACC ACCTGCCCCT ACAACTACCT CTCCACGGAA GTGGGATCCT GCACTCTGGT CTGTCCCCCG AACAACCAAG AGGTCACAGC TGAGGACGGA ACACAGCGGT GTGAGAAATG CAGCAAGCCC TGTGCTGGAG TATGCTATGG TCTGGGCATG GAGCACCTCC GAGGGGCGAG GGCCATCACC AGTGACAATA TCCAGGAGTT TGCTGGCTGC AAGAAGATCT TTGGGAGCCT GGCATTTTTG CCGGAGAGCT TTGATGGGAA CCCCTCCTCC GGCGTTGCCC CACTGAAGCC AGAGCATCTC CAAGTGTTCG AAACCCTGGA GGAGATCACA GGTTACCTAT ACATTTCAGC ATGGCCAGAG AGCTTCCAAG ACCTCAGTGT CTTCCAGAAC CTTCGGGTCA TTCGGGGACG GATTCTCCAT GATGGTGCTT ACTCATTGAC GTTGCAAGGC CTGGGGATTC ACTCACTGGG GCTACGCTCA CTGCGGGAGC TGGGCAGTGG ATTGGCTCTC ATTCACCGCA ACACCCATCT CTGCTTTGTA AACACTGTAC CTTGGGACCA GCTCTTCCGG AACCCGCACC AGGCCCTACT CCACAGTGGG AACCGGCCAG AAGAGGCATG TGGTCTTGAG GGCTTGGTCT GTAACTCACT GTGTGCCCGT GGGCACTGCT GGGGGCCAGG GCCCACCCAG TGTGTCAACT GCAGTCAGTT CCTCCGGGGC CAGGAGTGTG TGGAGGAGTG CCGAGTATGG AAGGGGCTCC CCAGGGAGTA TGTGAGGGGC AAGCACTGTC TGCCATGCCA CCCCGAGTGT CAGCCTCAAA ACAGCTCGGA GACCTGCTAT GGATCGGAGG CTGACCAGTG TGAGGCTTGT GCCCACTACA AGGACTCATC TTCCTGTGTG GCTCGCTGCC CCAGTGGTGT GAAGCCAGAC CTCTCCTACA TGCCTATCTG GAAGTACCCG GATGAGGAGG GCATATGTCA GCCATGCCCC ATCAACTGCA CCCACTCATG TGTGGACCTG GACGAACGAG GCTGCCCAGC AGAGCAGAGA GCCAGCCCAG TGACATTCAT CATTGCAACT GTGGTGGGCG TCCTGTTGTT CCTGATCATA GTGGTGGTCA TTGGAATCCT AATCAAACGA AGGCGACAGA AGATCCGGAA GTATACCATG CGTAGGCTGC TGCAGGAGAC CGAGCTGGTG GAGCCGCTGA CGCCCAGTGG AGCTGTGCCC AACCAGGCTC AGATGCGGAT CCTAAAGGAG ACAGAGCTAA GGAAGCTGAA GGTGCTTGGG TCAGGAGCCT TCGGCACTGT CTACAAGGGC ATCTGGATCC CAGATGGGGA GAACGTGAAA ATCCCCGTGG CCATCAAGGT GTTGAGGGAA AACACATCTC CTAAAGCTAA CAAAGAAATC CTAGATGAAG CGTACGTCAT GGCTGGTGTG GGTTCTCCAT ATGTGTCCCG CCTCCTGGGC ATCTGCCTGA CATCCACAGT GCAGCTGGTG ACACAGCTTA TGCCCTATGG CTGCCTTCTG GACCATGTCC GAGAACACCG AGGTCGCTTA GGCTCCCAGG ACCTGCTCAA CTGGTGTGTT CAGATTGCCA AGGGGATGAG CTACCTGGAG GAAGTTCGGC TTGTTCACAG GGACCTAGCT GCCCGAAACG TGCTAGTCAA GAGTCCCAAC CACGTCAAGA TTACCGACTT CGGGCTGGCA CGGCTGCTGG ACATTGATGA GACTGAATAC CATGCAGATG GGGGCAAGGT GCCCATCAAG TGGATGGCAT TGGAATCTAT TCTCAGACGC CGGTTCACCC ATCAGAGTGA TGTGTGGAGC TATGGTGTGA CTGTGTGGGA GCTGATGACC TTTGGGGCCA AACCTTACGA TGGGATCCCA GCTCGGGAGA TCCCTGATTT GCTGGAGAAG GGAGAACGCC TACCTCAGCC TCCAATCTGC ACCATCGACG TCTACATGAT CATGGTCAAA TGTTGGATGA TTGACTCCGA ATGTCGCCCG AGATTCCGGG AGTTGGTATC AGAATTCTCC CGTATGGCAA GGGACCCCCA GCGCTTTGTG GTCATCCAGA ACGAGGACTT AGGCCCCTCC AGCCCCATGG ACAGCACCTT CTACCGTTCA CTGCTGGAGG ATGATGACAT GGGGGAGCTG GTCGATGCTG AAGAGTACCT GGTACCCCAG CAGGGATTCT TCTCCCCAGA CCCTGCCCTA GGTACTGGGA GCACAGCCCA CCGCAGACAC CGCAGCTCGT CGGCCAGGAG TGGCGGTGGT GAGCTGACAC TGGGCCTGGA GCCCTCGGAA GAAGAGCCCC CCAGATCTCC ACTGGCTCCC TCCGAAGGGG CTGGCTCCGA TGTGTTTGAT GGTGACCTGG CAGTGGGGGT AACCAAAGGA CTGCAGAGCC TCTCTCCACA TGACCTCAGC CCTCTACAGC GGTACAGTGA GGATCCCACA TTACCTCTGC CCCCCGAGAC TGATGGCTAC GTTGCTCCCC TGGCCTGCAG CCCCCAGCCC GAGTATGTGA ACCAGCCAGA GGTTCGGCCT CAGTCTCCCT TGACCCCAGA GGGTCCTCCG CCTCCCATCC GACCTGCTGG TGCTACTCTA GAAAGACCCA AGACTCTCTC TCCTGGGAAA AATGGGGTTG TCAAAGACGT TTTTGCCTTT GGGGGTGCTG TGGAGAACCC TGAATACTTA GCACCCAGAG CAGGCACTGC CTCTCAGCCC CACCCTTCTC CTGCCTTCAG CCCAGCCTTT GACAACCTCT ATTACTGGGA CCAGAACTCA TCGGAGCAGG GTCCTCCACC AAGTACCTTT GAAGGGACCC CCACTGCAGA GAACCCTGAG TACCTAGGCC TGGATGTGCC AGTACTCGAG ACCGGTGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA TTACAAGGAT GACGATGACA AGTGACCGGT AAGTTTAAAC CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGGCC ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAT TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTC
