pENAAV-Itga2b(mouse)-FLAG
$113.00
In stock
SKU
PL41453
NM_010575.2;alphaIIb; CD41; CD41B; GpIIb
100μg liquid plasmid(Instantly):$134
| name | pENAAV-Itga2b(mouse)-FLAG | another name | apENAAV-Itga2b(mouse)-FLAG |
| Insert Size (bp) | 3102bp | Plasmid host | Mammary cell,Adeno-associated virus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTGCGCGCTC GCTCGCTCAC TGAGGCCGCC CGGGCAAAGC CCGGGCGTCG GGCGACCTTT GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT AGGGGTTCCT TGTAGTTAAT GATTAACCCG CCATGCTACT TATCTACCAG GGTAATGGGG ATCCTCTAGA ACTATAGCTA GAATTCGCCC TTACGGGCCC CCCCTCGAGG TCGGGATAAA AGCAGTCTGG GCTTTCACAT GACAGCATCT GGGGCTGCGG CAGAGGGTCG GGTCCGAAGC GCTGCCTTAT CAGCGTCCCC AGCCCTGGGA GGTGACAGCT GGCTGGCTTG TGTCAGCCCC TCGGGCACTC ACGTATCTCC GTCCGACGGG TTTAAAATAG CAAAACTCTG AGGCCACACA ATAGCTTGGG CTTATATGGG CTCCTGTGGG GGAAGGGGGA GCACGGAGGG GGCCGGGGCC GCTGCTGCCA AAATAGCAGC TCACAAGTGT TGCATTCCTC TCTGGGCGCC GGGCACATTC CTGCTGGCTC TGCCCGCCCC GGGGTGGGCG CCGGGGGGAC CTTAAAGCCT CTGCCCCCCA AGGAGCCCTT CCCAGACAGC CGCCGGCACC CACCGCTCCG TGGGACGATC CCCGAAGCTC TAGAGCTTTA TTGCGGTAGT TTATCACAGT TAAATTGCTA ACGCAGTCAG TGCTTCTGAC ACAACAGTCT CGAACTTAAG CTGCAGAAGT TGGTCGTGAG GCACTGGGCA GGTAAGTATC AAGGTTACAA GACAGGTTTA AGGAGACCAA TAGAAACTGG GCTTGTCGAG ACAGAGAAGA CTCTTGCGTT TCTGATAGGC ACCTATTGGT CTTACTGACA TCCACTTTGC CTTTCTCTCC ACAGGTGTCC ACTCCCAGTT CAATTACAGC TCTTAAGGCT AGAGTACTTA ATACGACTCA CTATAGGCTA GCCTCGAGAA TTCACGCGGG CCGCCATGGC CAGAGCCTCC TGCGCATGGC ATTCCCTGTG GCTTCTGCAG TGGACACCAC TGTTCTTGGG TCCTAGTGCT GTTCCTCCAG TCTGGGCCTT GAACCTGGAC TCTGAGAAGT TCTCCGTCTA TGCAGGTCCC AATGGCAGCC ACTTTGGCTT CTCAGTGGAC TTTCATAAGG ACAAACATGG AAGCGTGTCC ATCGTGGTGG GCGCCCCCAG GGCCCTAAAC GCAAGTCAAG AGGAGACTGG AGCCGTATTC CTGTGCCCCT GGAAGGCCAA TGGTGGCAAG TGCAACCCGT TGCTCTTCGA CCTCAGGGAT GAGACACGAA ACCTAGGCTT CCAAATTTTC CAAACCTTCA AGACCGGGCA AGGACTTGGG GCGTCGGTCG TCAGCTGGAA TGACGTCATT GTGGCCTGTG CCCCCTGGCA GCACTGGAAT GTCCTGGAAA AGCGCGATGA GGCAGAGAAG ACTCCGGTAG GAGGCTGCTT CTTGGCTCAG CTCCAAAGCG GGGGCCGCGC AGAGTACTCG CCCTGTCGCG CCAACACCAT GAGCTCCGTT TACGCAGAGA GTTTTCGCGG AGACAAGCGA TACTGTGAAG CAGGCTTCAG CTTGGCGGTG ACCCAGGCTG GGGAGCTAGT GCTTGGAGCC CCTGGAGGCT ACTTTTTTTT AGGTCTCCTG GCTCGCGTTC CAATTGAGAA CATCATCTCC AGCTACCGCC CGGGTACCCT TTTGTGGCAT GTTTCCAACC AGCGCTTCAC CTACGACAAC AGCAACCCAG TGTTTTTCGA TGGTTACCGG GGATATTCGG TAGCCGTGGG CGAGTTTGAT GGGGATCCGA GCACTACAGA GTACGTATCG GGTGCCCCCA CTTGGAGCTG GACCTTGGGA GCGGTGGAAA TTTTGGACTC CTACTACCAG CCCCTGCACC GGCTTCATGG AGAACAGATG GCTTCATATT TCGGGCACTC GGTGGCAGTC ACTGACGTCA ACGGGGACGG GAGGCATGAC CTACTGGTGG GGGCTCCATT GTATATGGAG AGCAGGGCAG ACCGCAAGCT GGCCGAGGTG GGCCGCGTTT ATTTGTTTCT GCAGCCTAAG GGCCCCCAAG CTCTGAGCAC ACCCACTCTC CTGCTGACTG GCACCCAGCT CTATGGGAGA TTTGGATCTG CCATTGCACC CCTGGGTGAC CTCAACCGAG ACGGCTATAA TGATATTGCT GTGGCTGCCC CCTATGGGGG TCCCAGTGGT CAGGGCCAAG TGCTGATATT CCTGGGTCAG AGTGAAGGGC TGAGTCCACG CCCCTCCCAG GTTCTGGACA GCCCCTTCCC CACAGGCTCT GGCTTTGGCT TCTCCCTTCG TGGTGCTGTA GACATCGATG ACAATGGATA CCCAGACCTG ATTGTGGGAG CATATGGAGC TAGCAAGGTG GCTGTGTACA GAGCTCAGCC TGTGGTGATG GCCACTGTCC AACTGATGGT TCAAGACTCC CTGAATCCAA CACTGAAGAA CTGTGTCCTG GATCAGACCA AGACACCAGT CAGCTGCTTC AACATCCAGA TGTGTGTGGG AGCCACAGGA CACAACATTC CTCAGAAGCT GCATCTAAAG GCAGAGCTGC AGCTGGACTT ACAGAAGCCC CGTCAGGGCC GCCGGGTGCT CCTGCTGGCA TCCCAACAGG CGAGCCTCAC CCTGAGCCTG GACCTGGGCG GAAGAGACAA GCCTATCTGC CACACCACGG GGGCCTTCCT TCGGGATGAG GCCGACTTCC GGGACAAGCT GAGCCCAATT GTGCTAAGCC TCAACGTGTC GCTGCCCCCA GAAGAGACTG GAGGAGCCCC TGCCGTGGTA TTGCATGGAG AAACCCATGT CCAGGAGCAG ACACGGATCA TCCTGGATTG TGGGGAAGAC GACCTGTGTG TGCCACAGCT CCGGCTCACA GCTACTGCGG GGGACTCCCC ACTCCTAATC GGTGCTGACA ATGTGTTGGA GCTGAAGATT GAAGCAGCCA ATGATGGTGA GGGAGCCTAT GAAGCGGAGC TGGCTGTGCA TCTGCCTCCA GGTGCCCACT ACATGCGGGC TCTCAGCAAC ATTGAGGGCT TTGAGAGGCT TGTCTGCACT CAGAAGAAAG AGAATGAGTC CAGGGTGGCC CTCTGTGAGC TGGGCAACCC CATGAAAAAG GACACCCGGA TAGGAATCAC AATGCTGGTG AGTGTGGAGA ACCTGGAAGA AGCTGGCGAG TCCGTGTCCT TCCAGCTGCA GGTCAGGAGC AAGAACAGCC AGAATCCAAA CAGTAAGGTC GTGATGTTGC CTGTGGCAAT CCAAGCTGAA GCCACAGTGG AGCTTCGAGG GAATTCCTTC CCTGCCTCCC TGGTGGTGGC AGCAGAAGAA GGTGACAGGG AGCAGGAAGA CTTGGACAGA TGGGTCTCCA GGCTGGAGCA CACCTATGAG CTCCACAACA TTGGCCCTGG CACTGTGAAT GGCCTCAGAC TTCTCATCCA CATTCCTGGC CAGTCCCAGC CCTCGGATCT GCTCTACATC CTGGATGTGC AGCCGCAGGG AGGTCTCCTT TGCTCCACAC AGCCATCTCC CAAGGTGGAC TGGAAACTAT CCACGCCCAG CCCTTCTTCC ATTCGCCCCG TCCATCACCA GCGTGAGCGC AGACAGGCAT TCCTGCAGGG GCCCAAGCCA GGGCAGCAGG ACCCAGTTCT GGTGAGCTGC GACGGCTCAG CGTCCTGTAC GGTGGTGGAG TGTGAGCTGC GGGAGATGGT GCGCGGGCAG CGGGCCATGG TTACTGTGCA GGTCATGCTG GGGCTGTCCA GCCTCCGCCA GAGGCCGCAG GAGCAGTTTG TGCTGCAGTC GCACGCCTGG TTCAACGTCT CCTCCCTACC TTACTCGGTG CCGGTGGTCA GCTTGCCCAG TGGGCAAGCT CGGGTGCAGA CACAGCTGCT TCGGGCCTTG GAGGAGAGGG CCATTCCTGT CTGGTGGGTG CTGGTGGGCG TGCTGGGCGG TCTGCTGCTG CTGACCCTGC TAGTTTTGGC CATGTGGAAG GCTGGCTTCT TCAAGCGGAA TCGACCGCCT CTGGAGGAAG ATGAAGAGGA AGAGGACTAC AAGGACGACG ATGACAAGTA GGTACCTCTA GAGTCGACCC GGGCGGCCTC GAGGACGGGG TGAACTACGC CTGAGGATCC GATCTTTTTC CCTCTGCCAA AAATTATGGG GACATCATGA AGCCCCTTGA GCATCTGACT TCTGGCTAAT AAAGGAAATT TATTTTCATT GCAATAGTGT GTTGGAATTT TTTGTGTCTC TCACTCGGAA GCAATTCGTT GATCTGAATT TCGACCACCC ATAATACCCA TTACCCTGGT AGATAAGTAG CATGGCGGGT TAATCATTAA CTACAAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGCTC GCTCGCTCAC TGAGGCCGGG CGACCAAAGG TCGCCCGACG CCCGGGCTTT GCCCGGGCGG CCTCAGTGAG CGAGCGAGCG CGCAGCCTTA ATTAACCTAA TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAGATTTAAT TAAGGCCTTA ATTAGG
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