RSF1010
$142.00
In stock
SKU
PL41280
100μg liquid plasmid(Instantly):$185
| name | RSF1010 | another name | aRSF1010 |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Str | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | RSF1010 | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACTGCACAT TCGGGATATT TCTCTATATT CGCGCTTCAT CAGAAAACTG AAGGAACCTC CATTGAATCG AACTAATATT TTTTTTGGTG AATCGCATTC TGACTGGTTG CCTGTCAGAG GCGGAGAATC TGGTGATTTT GTTTTTCGAC GTGGTGACGG GCATGCCTTC GCGAAAATCG CACCTGCTTC CCGCCGCGGT GAGCTCGCTG GAGAGCGTGA CCGCCTCATT TGGCTCAAAG GTCGAGGTGT GGCTTGCCCC GAGGTCATCA ACTGGCAGGA GGAACAGGAG GGTGCATGCT TGGTGATAAC GGCAATTCCG GGAGTACCGG CGGCTGATCT GTCTGGAGCG GATTTGCTCA AAGCGTGGCC GTCAATGGGG CAGCAACTTG GCGCTGTTCA CAGCCTATCG GTTGATCAAT GTCCGTTTGA GCGCAGGCTG TCGCGAATGT TCGGACGCGC CGTTGATGTG GTGTCCCGCA ATGCCGTCAA TCCCGACTTC TTACCGGACG AGGACAAGAG TACGCCGCTG CACGATCTTT TGGCTCGTGT CGAACGAGAG CTACCGGTGC GGCTCGACCA AGAGCGCACC GATATGGTTG TTTGCCATGG TGATCCCTGC ATGCCGAACT TCATGGTGGA CCCTAAAACT CTTCAATGCA CGGGTCTGAT CGACCTTGGG CGGCTCGGAA CAGCAGATCG CTATGCCGAT TTGGCACTCA TGATTGCTAA CGCCGAAGAG AACTGGGCAG CGCCAGATGA AGCAGAGCGC GCCTTCGCTG TCCTATTCAA TGTATTGGGG ATCGAAGCCC CCGACCGCGA ACGCCTTGCC TTCTATCTGC GATTGGACCC TCTGACTTGG GGTTGATGTT CATGCCGCCT GTTTTTCCTG CTCATTGGCA CGTTTCGCAA CCTGTTCTCA TTGCGGACAC CTTTTCCAGC CTCGTTTGGA AAGTTTCATT GCCAGACGGG ACTCCTGCAA TCGTCAAGGG ATTGAAACCT ATAGAAGACA TTGCTGATGA ACTGCGCGGG GCCGACTATC TGGTATGGCG CAATGGGAGG GGAGCAGTCC GGTTGCTCGG TCGTGAGAAC AATCTGATGT TGCTCGAATA TGCCGGGGAG CGAATGCTCT CTCACATCGT TGCCGAGCAC GGCGACTACC AGGCGACCGA AATTGCAGCG GAACTAATGG CGAAGCTGTA TGCCGCATCT GAGGAACCCC TGCCTTCTGC CCTTCTCCCG ATCCGGGATC GCTTTGCAGC TTTGTTTCAG CGGGCGCGCG ATGATCAAAA CGCAGGTTGT CAAACTGACT ACGTCCACGC GGCGATTATA GCCGATCAAA TGATGAGCAA TGCCTCGGAA CTGCGTGGGC TACATGGCGA TCTGCATCAT GAAAACATCA TGTTCTCCAG TCGCGGCTGG CTGGTGATAG ATCCCGTCGG TCTGGTCGGT GAAGTGGGCT TTGGCGCCGC CAATATGTTC TACGATCCGG CTGACAGAGA CGACCTTTGT CTCGATCCTA GACGCATTGC ACAGATGGCG GACGCATTCT CTCGTGCGCT GGACGTCGAT CCGCGTCGCC TGCTCGACCA GGCGTACGCT TATGGGTGCC TTTCCGCAGC TTGGAACGCG GATGGAGAAG AGGAGCAACG CGATCTAGCT ATCGCGGCCG CGATCAAGCA GGTGCGACAG ACGTCATACT AGATATCAAG CGACTTCTCC TATCCCCTGG GAACACATCA ATCTCACCGG AGAATATCGC TGGCCAAAGC CTTAGCGTAG GATTCCGCCC CTTCCCGCAA ACGACCCCAA ACAGGAAACG CAGCTGAAAC GGGAAGCTCA ACACCCACTG ACGCATGGGT TGTTCAGGCA GTACTTCATC AACCAGCAAG GCGGCACTTT CGGCCATCCG CCGCGCCCCA CAGCTCGGGC AGAAACCGCG ACGCTTACAG CTGAAAGCGA CCAGGTGCTC GGCGTGGCAA GACTCGCAGC GAACCCGTAG AAAGCCATGC TCCAGCCGCC CGCATTGGAG AAATTCTTCA AATTCCCGTT GCACATAGCC CGGCAATTCC TTTCCCTGCT CTGCCATAAG CGCAGCGAAT GCCGGGTAAT ACTCGTCAAC GATCTGATAG AGAAGGGTTT GCTCGGGTCG GTGGCTCTGG TAACGACCAG TATCCCGATC CCGGCTGGCC GTCCTGGCCG CCACATGAGG CATGTTCCGC GTCCTTGCAA TACTGTGTTT ACATACAGTC TATCGCTTAG CGGAAAGTTC TTTTACCCTC AGCCGAAATG CCTGCCGTTG CTAGACATTG CCAGCCAGTG CCCGTCACTC CCGTACTAAC TGTCACGAAC CCCTGCAATA ACTGTCACGC CCCCCTGCAA TAACTGTCAC GAACCCCTGC AATAACTGTC ACGCCCCCAA ACCTGCAAAC CCAGCAGGGG CGGGGGCTGG CGGGGTGTTG GAAAAATCCA TCCATGATTA TCTAAGAATA ATCCACTAGG CGCGGTTATC AGCGCCCTTG TGGGGCGCTG CTGCCCTTGC CCAATATGCC CGGCCAGAGG CCGGATAGCT GGTCTATTCG CTGCGCTAGG CTACACACCG CCCCACCGCT GCGCGGCAGG GGGAAAGGCG GGCAAAGCCC GCTAAACCCC ACACCAAACC CCGCAGAAAT ACGCTGGAGC GCTTTTAGCC GCTTTAGCGG CCTTTCCCCC TACCCGAAGG GTGGGGGCGC GTGTGCAGCC CCGCAGGGCC TGTCTCGGTC GATCATTCAG CCCGGCTCAT CCTTCTGGCG TGGCGGCAGA CCGAACAAGG CGCGGTCGTG GTCGCGTTCA AGGTACGCAT CCATTGCCGC CATGAGCCGA TCCTCCGGCC ACTCGCTGCT GTTCACCTTG GCCAAAATCA TGGCCCCCAC CAGCACCTTG CGCCTTGTTT CGTTCTTGCG CTCTTGCTGC TGTTCCCTTG CCCGCACCCG CTGAATTTCG GCATTGATTC GCGCTCGTTG TTCTTCGAGC TTGGCCAGCC GATCCGCCGC CTTGTTGCTC CCCTTAACCA TCTTGACACC CCATTGTTAA TGTGCTGTCT CGTAGGCTAT CATGGAGGCA CAGCGGCGGC AATCCCGACC CTACTTTGTA GGGGAGGGCG CACTTACCGG TTTCTCTTCG AGAAACTGGC CTAACGGCCA CCCTTCGGGC GGTGCGCTCT CCGAGGGCCA TTGCATGGAG CCGAAAAGCA AAAGCAACAG CGAGGCAGCA TGGCGATTTA TCACCTTACG GCGAAAACCG GCAGCAGGTC GGGCGGCCAA TCGGCCAGGG CCAAGGCCGA CTACATCCAG CGCGAAGGCA AGTATGCCCG CGACATGGAT GAAGTCTTGC ACGCCGAATC CGGGCACATG CCGGAGTTCG TCGAGCGGCC CGCCGACTAC TGGGATGCTG CCGACCTGTA TGAACGCGCC AATGGGCGGC TGTTCAAGGA GGTCGAATTT GCCCTGCCGG TCGAGCTGAC CCTCGACCAG CAGAAGGCGC TGGCGTCCGA GTTCGCCCAG CACCTGACCG GTGCCGAGCG CCTGCCGTAT ACGCTGGCCA TCCATGCCGG TGGCGGCGAG AACCCGCACT GCCACCTGAT GATCTCCGAG CGGATCAATG ACGGCATCGA GCGGCCCGCC GCTCAGTGGT TCAAGCGGTA CAACGGCAAG ACCCCGGAGA AGGGCGGGGC ACAGAAGACC GAAGCGCTCA AGCCCAAGGC ATGGCTTGAG CAGACCCGCG AGGCATGGGC CGACCATGCC AACCGGGCAT TAGAGCGGGC TGGCCACGAC GCCCGCATTG ACCACAGAAC ACTTGAGGCG CAGGGCATCG AGCGCCTGCC CGGTGTTCAC CTGGGGCCGA ACGTGGTGGA GATGGAAGGC CGGGGCATCC GCACCGACCG GGCAGACGTG GCCCTGAACA TCGACACCGC CAACGCCCAG ATCATCGACT TACAGGAATA CCGGGAGGCA ATAGACCATG AACGCAATCG ACAGAGTGAA GAAATCCAGA GGCATCAACG AGTTAGCGGA GCAGATCGAA CCGCTGGCCC AGAGCATGGC GACACTGGCC GACGAAGCCC GGCAGGTCAT GAGCCAGACC CAGCAGGCCA GCGAGGCGCA GGCGGCGGAG TGGCTGAAAG CCCAGCGCCA GACAGGGGCG GCATGGGTGG AGCTGGCCAA AGAGTTGCGG GAGGTAGCCG CCGAGGTGAG CAGCGCCGCG CAGAGCGCCC GGAGCGCGTC GCGGGGGTGG CACTGGAAGC TATGGCTAAC CGTGATGCTG GCTTCCATGA TGCCTACGGT GGTGCTGCTG ATCGCATCGT TGCTCTTGCT CGACCTGACG CCACTGACAA CCGAGGACGG CTCGATCTGG CTGCGCTTGG TGGCCCGATG AAGAACGACA GGACTTTGCA GGCCATAGGC CGACAGCTCA AGGCCATGGG CTGTGAGCGC TTCGATATCG GCGTCAGGGA CGCCACCACC GGCCAGATGA TGAACCGGGA ATGGTCAGCC GCCGAAGTGC TCCAGAACAC GCCATGGCTC AAGCGGATGA ATGCCCAGGG CAATGACGTG TATATCAGGC CCGCCGAGCA GGAGCGGCAT GGTCTGGTGC TGGTGGACGA CCTCAGCGAG TTTGACCTGG ATGACATGAA AGCCGAGGGC CGGGAGCCTG CCCTGGTAGT GGAAACCAGC CCGAAGAACT ATCAGGCATG GGTCAAGGTG GCCGACGCCG CAGGCGGTGA ACTTCGGGGG CAGATTGCCC GGACGCTGGC CAGCGAGTAC GACGCCGACC CGGCCAGCGC CGACAGCCGC CACTATGGCC GCTTGGCGGG CTTCACCAAC CGCAAGGACA AGCACACCAC CCGCGCCGGT TATCAGCCGT GGGTGCTGCT GCGTGAATCC AAGGGCAAGA CCGCCACCGC TGGCCCGGCG CTGGTGCAGC AGGCTGGCCA GCAGATCGAG CAGGCCCAGC GGCAGCAGGA GAAGGCCCGC AGGCTGGCCA GCCTCGAACT GCCCGAGCGG CAGCTTAGCC GCCACCGGCG CACGGCGCTG GACGAGTACC GCAGCGAGAT GGCCGGGCTG GTCAAGCGCT TCGGTGATGA CCTCAGCAAG TGCGACTTTA TCGCCGCGCA GAAGCTGGCC AGCCGGGGCC GCAGTGCCGA GGAAATCGGC AAGGCCATGG CCGAGGCCAG CCCAGCGCTG GCAGAGCGCA AGCCCGGCCA CGAAGCGGAT TACATCGAGC GCACCGTCAG CAAGGTCATG GGTCTGCCCA GCGTCCAGCT TGCGCGGGCC GAGCTGGCAC GGGCACCGGC ACCCCGCCAG CGAGGCATGG ACAGGGGCGG GCCAGATTTC AGCATGTAGT GCTTGCGTTG GTACTCACGC CTGTTATACT ATGAGTACTC ACGCACAGAA GGGGGTTTTA TGGAATACGA AAAAAGCGCT TCAGGGTCGG TCTACCTGAT CAAAAGTGAC AAGGGCTATT GGTTGCCCGG TGGCTTTGGT TATACGTCAA ACAAGGCCGA GGCTGGCCGC TTTTCAGTCG CTGATATGGC CAGCCTTAAC CTTGACGGCT GCACCTTGTC CTTGTTCCGC GAAGACAAGC CTTTCGGCCC CGGCAAGTTT CTCGGTGACT GATATGAAAG ACCAAAAGGA CAAGCAGACC GGCGACCTGC TGGCCAGCCC TGACGCTGTA CGCCAAGCGC GATATGCCGA GCGCATGAAG GCCAAAGGGA TGCGTCAGCG CAAGTTCTGG CTGACCGACG ACGAATACGA GGCGCTGCGC GAGTGCCTGG AAGAACTCAG AGCGGCGCAG GGCGGGGGTA GTGACCCCGC CAGCGCCTAA CCACCAACTG CCTGCAAAGG AGGCAATCAA TGGCTACCCA TAAGCCTATC AATATTCTGG AGGCGTTCGC AGCAGCGCCG CCACCGCTGG ACTACGTTTT GCCCAACATG GTGGCCGGTA CGGTCGGGGC GCTGGTGTCG CCCGGTGGTG CCGGTAAATC CATGCTGGCC CTGCAACTGG CCGCACAGAT TGCAGGCGGG CCGGATCTGC TGGAGGTGGG CGAACTGCCC ACCGGCCCGG TGATCTACCT GCCCGCCGAA GACCCGCCCA CCGCCATTCA TCACCGCCTG CACGCCCTTG GGGCGCACCT CAGCGCCGAG GAACGGCAAG CCGTGGCTGA CGGCCTGCTG ATCCAGCCGC TGATCGGCAG CCTGCCCAAC ATCATGGCCC CGGAGTGGTT CGACGGCCTC AAGCGCGCCG CCGAGGGCCG CCGCCTGATG GTGCTGGACA CGCTGCGCCG GTTCCACATC GAGGAAGAAA ACGCCAGCGG CCCCATGGCC CAGGTCATCG GTCGCATGGA GGCCATCGCC GCCGATACCG GGTGCTCTAT CGTGTTCCTG CACCATGCCA GCAAGGGCGC GGCCATGATG GGCGCAGGCG ACCAGCAGCA GGCCAGCCGG GGCAGCTCGG TACTGGTCGA TAACATCCGC TGGCAGTCCT ACCTGTCGAG CATGACCAGC GCCGAGGCCG AGGAATGGGG TGTGGACGAC GACCAGCGCC GGTTCTTCGT CCGCTTCGGT GTGAGCAAGG CCAACTATGG CGCACCGTTC GCTGATCGGT GGTTCAGGCG GCATGACGGC GGGGTGCTCA AGCCCGCCGT GCTGGAGAGG CAGCGCAAGA GCAAGGGGGT GCCCCGTGGT GAAGCCTAAG AACAAGCACA GCCTCAGCCA CGTCCGGCAC GACCCGGCGC ACTGTCTGGC CCCCGGCCTG TTCCGTGCCC TCAAGCGGGG CGAGCGCAAG CGCAGCAAGC TGGACGTGAC GTATGACTAC GGCGACGGCA AGCGGATCGA GTTCAGCGGC CCGGAGCCGC TGGGCGCTGA TGATCTGCGC ATCCTGCAAG GGCTGGTGGC CATGGCTGGG CCTAATGGCC TAGTGCTTGG CCCGGAACCC AAGACCGAAG GCGGACGGCA GCTCCGGCTG TTCCTGGAAC CCAAGTGGGA GGCCGTCACC GCTGAATGCC ATGTGGTCAA AGGTAGCTAT CGGGCGCTGG CAAAGGAAAT CGGGGCAGAG GTCGATAGTG GTGGGGCGCT CAAGCACATA CAGGACTGCA TCGAGCGCCT TTGGAAGGTA TCCATCATCG CCCAGAATGG CCGCAAGCGG CAGGGGTTTC GGCTGCTGTC GGAGTACGCC AGCGACGAGG CGGACGGGCG CCTGTACGTG GCCCTGAACC CCTTGATCGC GCAGGCCGTC ATGGGTGGCG GCCAGCATGT GCGCATCAGC ATGGACGAGG TGCGGGCGCT GGACAGCGAA ACCGCCCGCC TGCTGCACCA GCGGCTGTGT GGCTGGATCG ACCCCGGCAA AACCGGCAAG GCTTCCATAG ATACCTTGTG CGGCTATGTC TGGCCGTCAG AGGCCAGTGG TTCGACCATG CGCAAGCGCC GCCAGCGGGT GCGCGAGGCG TTGCCGGAGC TGGTCGCGCT GGGCTGGACG GTAACCGAGT TCGCGGCGGG CAAGTACGAC ATCACCCGGC CCAAGGCGGC AGGCTGACCC CCCCCACTCT ATTGTAAACA AGACATTTTT ATCTTTTATA TTCAATGGCT TATTTTCCTG CTAATTGGTA ATACCATGAA AAATACCATG CTCAGAAAAG GCTTAACAAT ATTTTGAAAA ATTGCCTACT GAGCGCTGCC GCACAGCTCC ATAGGCCGCT TTCCTGGCTT TGCTTCCAGA TGTATGCTCT TCTGCTCCTG CAGCTAATGG ATCACCGCAA ACAGGTTACT CGCCTGGGGA TTCCCTTTCG ACCCGAGCAT CCGTATGATA CTCATGCTCG ATTATTATTA TTATAGAAGC CCCCATGAAT AAATCGCTCA TCATTTTCGG CATCGTCAAC ATAACCTCGG ACAGTTTCTC CGATGGAGGC CGGTATCTGG CGCCAGACGC AGCCATTGCG CAGGCGCGTA AGCTGATGGC CGAGGGGGCA GATGTGATCG ACCTGGTCCG GCATCCAGCA ATCCCGACGC CGCGCCTGTT TCGTCCGACA CAGAAATCGC GCGTATGCGC CGGTGCTGGA CGCGCTCAGG CAGATGGCAT TCCCGTCTCG CTCGACAGTT ATCAACCCGC GACGCAAGCC TATGCCTTGT CGCGTGGTGT GGCCTATCTC AATGATATTC GCGGTTTTCC AGACGCTGCG TTCTATCCGC AATTGGCGAA ATCATCTGCC AAACTCGTCG TTATGCATTC GGTGCAAGAC GGGCAGGCAG ATCGGCGCGA GGCACCCGCT GGCGACATCA TGGATCACAT TGCGGCGTTC TTTGACGCGC GCATCGCGGC GCTGACGGGT GCCGGTATCA AACGCAACCG CCTTGTCCTT GATCCCGGCA TGGGGTTTTT TCTGGGGGCT GCTCCCGAAA CCTCGCTCTC GGTGCTGGCG CGGTTCGATG AATTGCGGCT GCGCTTCGAT TTGCCGGTGC TTCTGTCTGT TTCGCGCAAA TCCTTTCTGC GCGCGCTCAC AGGCCGTGGT CCGGGGGTGT CGGGGCCGCG ACACTCGCTG CAGAGCTTGC CGCCGCCGCA GGTGGAGCTG ACTTCATCCG CACACACGAG CCGCGCCCCT TGCGCGACGG GCTGGCGGTA TTGGCGGCGC TGAAAGAAAC CGCAAGAATT CGTT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
