p21(human)-Fluc
$70.00
In stock
SKU
PL41147
100μg liquid plasmid(Instantly):$91
| name | p21(human)-Fluc | another name | ap21(human)-Fluc |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Fluc | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATGGTACTG TAACTGAGCT AACATAACCC GGGAGGTACC GCTTGGGCAG CAGGCTGTGG CTCTGATTGG CTTTCTGGCC ATTAGGAACA TGTCCCAACA TGTTGAGCTC TGGCATAGAA GAGGCTGGTG GCTATTTTGT CCTTGGGCTG CCTGTTATCA GGTGAGGAAG GGGATGGTAG GAGACAGAAG ACCTCTAAAG GCCCCAGGTA AGCCTTAGCC TGTTACTCTG AACAGGGTAT GTGATCTGCC AGCAGATCCT TGCGACAGGG CTGGGATCTG ATGCATGTGT GCTTGTGTGA GTGTGTGCTG GGAGTCAGAT TCTGTGTGTG ACTTTTAACA GCCTGCTCCC TTGCCTTCCT CAGGGCAGAA GTCCTCCCTT AGAGTGTGTC TGGGTACACA TTCAAGTGCA TGGTTGCAAA CTTTTTTTTT TAAAGCACTG AATAGTACTA GACACTTAGT AGGTACTTAA GAAATATTGA ATGTCGTGGT GGTGGTGAGC TAGAAGTTAT AAAAAAAATT CTTTCCCAAA AACAACAACA AAAAGAATTA TTTCATTGTG AAGCTCAGTA CCACAAAAAT TTAAATAATT CATTACAAGC CTTCATTAAA AAAAAATTTC TCCCCGAAGT AAACAGACAG ACAATGTCTA GTCTATTTGA AATGCCTGAA AGCAGAGGGG CTTCAAGGCA GTGGGAGAAG GTGCCTGTCC TCTGCTGGAC ATTTGACAAC CAGCCCTTTG GATGGCTTGT ATGTATAGGA GCGAAGGTGC AGACAGCAGT GGGGCTTAGA GTGGGGTCCT GAGGCTGTGC TGTGGCCCTT CTGGGGTTTA GCCACAATCC TGGCCTGACT CCAGGGCGAG GCAGGCCAAG GGGGCCTGCT GCTGTGTCCT CCCACCCCTA CCTGGGCTCC CATCCCCACA GCAGAGGAGA AAGAAGCCTG TCCTCCCCGA GGTCAGCTGC GTTAGAGGAA GAAGACTGGG CATGTCTGGG CAGAGATTTC CAGACTCTGA GCAGCCTGAG ATGTCAGTAA TTGTAGCTGC TCCAAGCCTG GGTTCTGTTT TTCAGTGGGA TTTCTGTTCA GATGAACAAT CCATCCTCTG CAATTTTTTA AAAGCAAAAC TGCAAATGTT TCAGGCACAG AAAGGAGGCA AAGGTGAAGT CCAGGGGAGG TCAGGGGTGT GAGGTAGATG GGAGCGGATA GGCACATCAC TCATTTCTGT GTCTGTCAGA AGAACCAGTA GACACTTCCA GAATTGTCCT TTATTTACGT CATCTCCATA AACCATCTGC AAATGAGGGT TATTTGGCAT TTTTGTCATT TTGGAACCAC AGAAATAAAG GATGACAAGC AGAGAGCCCC GGGCAGGAGG CAAAAGTCCT GTGTTCCAAC TATAGTCATT TCTTTGCTGC ATGATCTGAG TTAGGTCACC AGACTTCTCT GAGCCCCAGT TTCCCCAGCA GTGTATACGG GCTATGTGGG GAGTATTCAG GAGACAGACA ACTCACTCGT CAAATCCTCC CCTTCCTGGC CAACAAAGCT GCTGCAACCA CAGGGGTTTC TTCTGTTCAG GTGAGTGTAG GGTGTAGGGA GATTGGTTCA ATGTCCAATT CTTCTGTTTC CCTGGAGATC AGGTTGCCCT TTTTTGGTAG TCTCTCCAAT TCCCTCCTTC CCGGAAGCAT GTGACAATCA ACAACTTTGT ATACTTAAGT TCAGTGGACC TCAATTTCCT CATCTGTGAA ATAAACGGGA CTGAAAAATC ATTCCGGCCT CAAGATGCTT TGTTGGGGTG TCTAGGTGCT CCAGGTGCTT CTGGGAGAGG TGACCTAGTG AGGGATCAGT GGGAATAGAG GTGATATTGT GGGGCTTTTC TGGAAATTGC AGAGAGGTGC ATCGTTTTTA TAATTTATGA ATTTTTATGT ATTAATGTCA TCCTCCTGAT CTTTTCAGCT GCATTGGGTA AATCCTTGCC TGCCAGAGTG GGTCAGCGGT GAGCCAGAAA GGGGGCTCAT TCTAACAGTG CTGTGTCCTC CTGGAGAGTG CCAACTCATT CTCCAAGTAA AAAAAGCCAG ATTTGTGGCT CACTTCGTGG GGAAATGTGT CCAGCGCACC AACGCAGGCG AGGGACTGGG GGAGGAGGGA AGTGCCCCTC TGCAGCACGC GAGGTTCCGG GACCGGCTGG CCTGCTGGAA CTCGGCCAGG CTCAGCTGGC TCCGCGCTGG GCAGCCAGGA GCCTGGGCCC CGGGGAGGGC GGTCCCGGGC GGCGCGGTGG GCCGAGCGCG GGTCCCGCCT CCTTGAGGCG GGCCCGGGCG GGGCGGTTGT ATATCAGGGC CGCGCAGAGC TGCGCCAGCT GAGGTGTGAG CAGCTGCCGA AGTCAGCTCG AGATCTAAGT AAGCTTGGCA TTCCGGTACT GTTGGTAAAA TGGAAGACGC CAAAAACATA AAGAAAGGCC CGGCGCCATT CTATCCTCTA GAGGATGGAA CCGCTGGAGA GCAACTGCAT AAGGCTATGA AGAGATACGC CCTGGTTCCT GGAACAATTG CTTTTACAGA TGCACATATC GAGGTGAACA TCACGTACGC GGAATACTTC GAAATGTCCG TTCGGTTGGC AGAAGCTATG AAACGATATG GGCTGAATAC AAATCACAGA ATCGTCGTAT GCAGTGAAAA CTCTCTTCAA TTCTTTATGC CGGTGTTGGG CGCGTTATTT ATCGGAGTTG CAGTTGCGCC CGCGAACGAC ATTTATAATG AACGTGAATT GCTCAACAGT ATGAACATTT CGCAGCCTAC CGTAGTGTTT GTTTCCAAAA AGGGGTTGCA AAAAATTTTG AACGTGCAAA AAAAATTACC AATAATCCAG AAAATTATTA TCATGGATTC TAAAACGGAT TACCAGGGAT TTCAGTCGAT GTACACGTTC GTCACATCTC ATCTACCTCC CGGTTTTAAT GAATACGATT TTGTACCAGA GTCCTTTGAT CGTGACAAAA CAATTGCACT GATAATGAAT TCCTCTGGAT CTACTGGGTT ACCTAAGGGT GTGGCCCTTC CGCATAGAAC TGCCTGCGTC AGATTCTCGC ATGCCAGAGA TCCTATTTTT GGCAATCAAA TCATTCCGGA TACTGCGATT TTAAGTGTTG TTCCATTCCA TCACGGTTTT GGAATGTTTA CTACACTCGG ATATTTGATA TGTGGATTTC GAGTCGTCTT AATGTATAGA TTTGAAGAAG AGCTGTTTTT ACGATCCCTT CAGGATTACA AAATTCAAAG TGCGTTGCTA GTACCAACCC TATTTTCATT CTTCGCCAAA AGCACTCTGA TTGACAAATA CGATTTATCT AATTTACACG AAATTGCTTC TGGGGGCGCA CCTCTTTCGA AAGAAGTCGG GGAAGCGGTT GCAAAACGCT TCCATCTTCC AGGGATACGA CAAGGATATG GGCTCACTGA GACTACATCA GCTATTCTGA TTACACCCGA GGGGGATGAT AAACCGGGCG CGGTCGGTAA AGTTGTTCCA TTTTTTGAAG CGAAGGTTGT GGATCTGGAT ACCGGGAAAA CGCTGGGCGT TAATCAGAGA GGCGAATTAT GTGTCAGAGG ACCTATGATT ATGTCCGGTT ATGTAAACAA TCCGGAAGCG ACCAACGCCT TGATTGACAA GGATGGATGG CTACATTCTG GAGACATAGC TTACTGGGAC GAAGACGAAC ACTTCTTCAT AGTTGACCGC TTGAAGTCTT TAATTAAATA CAAAGGATAT CAGGTGGCCC CCGCTGAATT GGAATCGATA TTGTTACAAC ACCCCAACAT CTTCGACGCG GGCGTGGCAG GTCTTCCCGA CGATGACGCC GGTGAACTTC CCGCCGCCGT TGTTGTTTTG GAGCACGGAA AGACGATGAC GGAAAAAGAG ATCGTGGATT ACGTGGCCAG TCAAGTAACA ACCGCGAAAA AGTTGCGCGG AGGAGTTGTG TTTGTGGACG AAGTACCGAA AGGTCTTACC GGAAAACTCG ACGCAAGAAA AATCAGAGAG ATCCTCATAA AGGCCAAGAA GGGCGGAAAG TCCAAATTGT AAAATGTAAC TGTATTCAGC GATGACGAAA TTCTTAGCTA TTGTAATACT GCGATGAGTG GCAGGGCGGG GCGTAATTTT TTTAAGGCAG TTATTGGTGC CCTTAAACGC CTGGTGCTAC GCCTGAATAA GTGATAATAA GCGGATGAAT GGCAGAAATT CGCCGGATCT TTGTGAAGGA ACCTTACTTC TGTGGTGTGA CATAATTGGA CAAACTACCT ACAGAGATTT AAAGCTCTAA GGTAAATATA AAATTTTTAA GTGTATAATG TGTTAAACTA CTGATTCTAA TTGTTTGTGT ATTTTAGATT CCAACCTATG GAACTGATGA ATGGGAGCAG TGGTGGAATG CCTTTAATGA GGAAAACCTG TTTTGCTCAG AAGAAATGCC ATCTAGTGAT GATGAGGCTA CTGCTGACTC TCAACATTCT ACTCCTCCAA AAAAGAAGAG AAAGGTAGAA GACCCCAAGG ACTTTCCTTC AGAATTGCTA AGTTTTTTGA GTCATGCTGT GTTTAGTAAT AGAACTCTTG CTTGCTTTGC TATTTACACC ACAAAGGAAA AAGCTGCACT GCTATACAAG AAAATTATGG AAAAATATTC TGTAACCTTT ATAAGTAGGC ATAACAGTTA TAATCATAAC ATACTGTTTT TTCTTACTCC ACACAGGCAT AGAGTGTCTG CTATTAATAA CTATGCTCAA AAATTGTGTA CCTTTAGCTT TTTAATTTGT AAAGGGGTTA ATAAGGAATA TTTGATGTAT AGTGCCTTGA CTAGAGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCCGTC GACCGATGCC CTTGAGAGCC TTCAACCCAG TCAGCTCCTT CCGGTGGGCG CGGGGCATGA CTATCGTCGC CGCACTTATG ACTGTCTTCT TTATCATGCA ACTCGTAGGA CAGGTGCCGG CAGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGTTTAC AATTTCCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCCCA AGCTACCATG ATAAGTAAGT AATATTAAGG TACGTGGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT TGTT
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