NM_004239.4;ACG1A; CEV14; GMAP-210; GMAP210; ODCD;ODCD1; TRIP-11; TRIP230
100μg liquid plasmid(Instantly):$149
| name | pLV3-CMV-6×His-TRIP11(human)-EGFP-Puro | another name | apLV3-CMV-6×His-TRIP11(human)-EGFP-Puro |
| Insert Size (bp) | 5940bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCGCGCAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGA GCTGCAAGCT TAATGTAGTC TTATGCAATA CTCTTGTAGT CTTGCAACAT GGTAACGATG AGTTAGCAAC ATGCCTTACA AGGAGAGAAA AAGCACCGTG CATGCCGATT GGTGGAAGTA AGGTGGTACG ATCGTGCCTT ATTAGGAAGG CAACAGACGG GTCTGACATG GATTGGACGA ACCACTGAAT TGCCGCATTG CAGAGATATT GTATTTAAGT GCCTAGCTCG ATACATAAAC GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCGCTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTTGG AATCACACGA CCTGGATGGA GTGGGACAGA GAAATTAACA ATTACACAAG CTTAATACAC TCCTTAATTG AAGAATCGCA AAACCAGCAA GAAAAGAATG AACAAGAATT ATTGGAATTA GATAAATGGG CAAGTTTGTG GAATTGGTTT AACATAACAA ATTGGCTGTG GTATATAAAA TTATTCATAA TGATAGTAGG AGGCTTGGTA GGTTTAAGAA TAGTTTTTGC TGTACTTTCT ATAGTGAATA GAGTTAGGCA GGGATATTCA CCATTATCGT TTCAGACCCA CCTCCCAACC CCGAGGGGAC CCGACAGGCC CGAAGGAATA GAAGAAGAAG GTGGAGAGAG AGACAGAGAC AGATCCATTC GATTAGTGAA CGGATCTCGA CGGTATCGGT TAACTTTTAA AAGAAAAGGG GGGATTGGGG GGTACAGTGC AGGGGAAAGA ATAGTAGACA TAATAGCAAC AGACATACAA ACTAAAGAAT TACAAAAACA AATTACAAAA TTCAAAATTT TATCGATAAG CTTGGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGA CTCTAGAGCC ACCATGCACC ATCACCATCA CCATATGTCG TCCTGGCTTG GGGGCCTCGG CTCCGGATTG GGCCAGTCTC TGGGTCAAGT CGGGGGCAGC CTGGCTTCCC TCACTGGCCA GATATCAAAC TTTACAAAGG ATATGCTGAT GGAGGGCACG GAGGAAGTGG AAGCAGAATT ACCTGATTCT AGGACAAAGG AAATTGAAGC CATTCATGCA ATCTTGAGAT CAGAGAATGA AAGGCTTAAG AAACTTTGTA CTGATCTAGA AGAGAAACAT GAAGCATCAG AGATTCAAAT AAAGCAGCAA TCTACAAGTT ACCGAAATCA ACTTCAACAA AAAGAGGTAG AAATCAGCCA TCTTAAAGCC AGACAGATTG CACTCCAGGA TCAGTTGCTG AAACTGCAGT CAGCTGCTCA GTCAGTACCT TCAGGAGCTG GTGTACCAGC AACCACTGCA TCATCTTCAT TCGCTTATGG GATTAGTCAT CATCCTTCAG CTTTCCATGA CGATGACATG GACTTTGGTG ATATAATTTC ATCCCAACAA GAAATAAACC GACTCTCAAA TGAAGTTTCA AGACTTGAGT CTGAAGTTGG CCATTGGAGG CATATTGCTC AGACTTCCAA AGCACAAGGA ACAGATAACT CTGATCAAAG TGAAATATGT AAACTACAAA ATATCATTAA GGAACTAAAA CAGAACCGAA GTCAGGAAAT TGATGACCAT CAACATGAAA TGTCAGTACT GCAGAATGCA CACCAACAGA AATTGACAGA AATAAGTCGA CGACATCGAG AAGAATTAAG TGACTATGAA GAACGAATTG AAGAACTTGA AAATCTGTTA CAACAAGGTG GCTCTGGAGT TATAGAAACT GATCTCTCTA AAATCTATGA GATGCAAAAA ACTATTCAAG TTCTACAAAT AGAAAAAGTG GAGTCTACCA AAAAAATGGA ACAACTTGAG GATAAAATAA AAGATATAAA TAAAAAATTA TCTTCTGCAG AAAATGACAG AGATATTTTG AGGAGAGAAC AAGAACAGCT AAATGTGGAA AAGAGACAAA TAATGGAAGA ATGTGAAAAC TTGAAATTGG AATGTAGTAA ATTGCAGCCT TCTGCTGTGA AGCAAAGTGA TACTATGACA GAAAAGGAAA GAATTCTTGC CCAGAGTGCA TCAGTGGAAG AAGTGTTCAG ACTACAACAA GCACTGTCTG ATGCCGAAAA TGAAATAATG AGATTGAGTA GTTTAAACCA GGATAACAGT CTTGCTGAAG ACAATCTGAA ACTTAAAATG CGTATCGAAG TTTTAGAAAA AGAGAAGTCA TTACTGAGTC AAGAAAAGGA AGAACTTCAG ATGTCACTTT TAAAATTGAA CAATGAATAT GAAGTAATTA AAAGTACAGC TACAAGAGAC ATAAGTTTGG ATTCAGAATT ACATGACTTA AGACTTAATT TGGAGGCAAA GGAACAAGAA CTCAATCAGA GTATTAGTGA AAAGGAAACA CTGATAGCTG AGATAGAAGA ATTGGACAGA CAGAATCAAG AAGCTACAAA GCACATGATT TTGATAAAAG ATCAGCTATC AAAACAACAA AATGAAGGAG ATAGCATCAT CAGTAAACTG AAACAAGATC TAAATGATGA AAAAAAGAGA GTTCATCAAC TTGAAGATGA TAAAATGGAC ATTACTAAAG AGTTAGATGT ACAGAAAGAA AAGCTAATTC AAAGTGAAGT GGCCCTAAAT GATTTACATT TAACCAAGCA GAAACTTGAG GACAAAGTAG AAAATTTAGT AGATCAGCTA AATAAATCAC AAGAAAGTAA TGTAAGCATC CAGAAGGAGA ATTTAGAACT TAAGGAGCAT ATTAGACAAA ATGAGGAGGA GCTTTCTAGA ATAAGGAATG AGTTAATGCA GTCTCTAAAT CAAGACTCTA ATAGTAATTT TAAGGATACC TTACTTAAAG AAAGAGAAGC TGAAGTTAGA AACTTAAAGC AAAATCTTTC AGAATTAGAA CAGCTCAATG AAAATTTAAA GAAAGTTGCT TTTGATGTCA AAATGGAAAA TGAAAAGTTA GTTTTAGCAT GTGAAGATGT GAGGCATCAG TTAGAAGAAT GTCTTGCTGG TAACAATCAG CTTTCTCTGG AAAAAAACAC TATTGTGGAG ACTCTAAAAA TGGAAAAAGG AGAGATAGAG GCAGAATTGT GTTGGGCTAA AAAGAGGCTG TTGGAAGAAG CAAACAAGTA TGAGAAAACC ATTGAAGAAC TGTCAAATGC ACGTAATTTG AATACCTCTG CCTTACAGCT GGAACATGAG CATTTAATTA AACTCAATCA AAAGAAAGAC ATGGAAATAG CAGAACTCAA AAAGAATATT GAACAAATGG ATACTGACCA TAAAGAAACT AAGGACGTTT TGTCATCTAG TTTAGAAGAG CAGAAGCAGT TGACACAACT TATAAACAAG AAAGAAATTT TTATTGAAAA GCTTAAAGAA AGAAGTTCAA AGCTGCAGGA GGAATTGGAT AAATATTCTC AGGCCTTAAG AAAAAATGAA ATTTTAAGAC AGACCATAGA GGAAAAAGAC CGAAGTCTTG GATCCATGAA AGAGGAAAAT AATCATCTGC AAGAAGAATT GGAACGACTC AGGGAAGAGC AGAGTCGAAC CGCACCTGTG GCTGACCCTA AAACCCTTGA TAGTGTTACT GAACTAGCAT CTGAGGTATC TCAACTGAAC ACGATCAAGG AACATCTTGA AGAGGAAATT AAACATCATC AAAAGATAAT TGAAGATCAA AACCAGAGTA AGATGCAACT ACTTCAGTCT TTACAAGAGC AAAAGAAGGA AATGGATGAG TTTAGATACC AGCATGAGCA AATGAACGCC ACACACACCC AGCTCTTTTT AGAGAAGGAT GAGGAAATTA AGAGTTTGCA AAAAACAATT GAACAAATCA AAACCCAGTT GCATGAAGAA AGACAGGACA TTCAAACAGA TAACTCTGAT ATTTTTCAAG AAACAAAAGT TCAGAGCCTT AATATAGAAA ATGGAAGTGA AAAGCATGAT TTATCTAAAG CTGAAACGGA AAGATTAGTG AAAGGAATAA AAGAGCGAGA ACTGGAGATT AAACTTCTAA ATGAAAAGAA TATATCTTTA ACTAAACAGA TTGATCAGTT GTCCAAAGAT GAAGTTGGTA AACTAACTCA GATTATTCAG CAGAAAGATT TGGAGATACA AGCTCTTCAT GCTAGAATTT CTTCAACTTC CCATACTCAA GATGTTGTTT ACCTTCAACA GCAACTGCAG GCTTATGCTA TGGAAAGAGA AAAGGTATTT GCTGTTTTGA ATGAGAAGAC TAGGGAAAAT AGCCATCTAA AAACAGAATA TCACAAAATG ATGGATATTG TTGCTGCCAA GGAAGCAGCT CTTATCAAAC TGCAAGATGA AAATAAAAAA TTGTCCACTA GATTTGAAAG TAGTGGCCAA GATATGTTTA GAGAAACTAT TCAGAATTTA TCACGTATCA TTCGAGAAAA AGACATCGAA ATAGATGCAC TAAGTCAGAA ATGTCAGACT TTATTGGCAG TTTTACAAAC ATCCAGCACT GGTAATGAGG CTGGAGGTGT TAATAGTAAT CAATTTGAGG AGCTTCTACA GGAACGTGAC AAGTTAAAAC AGCAAGTAAA GAAAATGGAA GAGTGGAAGC AGCAGGTGAT GACCACAGTA CAAAATATGC AACACGAGTC AGCCCAGCTT CAGGAAGAGC TTCACCAACT TCAAGCACAG GTTTTGGTTG ACAGTGATAA TAATTCTAAA TTACAAGTGG ACTATACTGG CCTGATCCAA AGTTATGAGC AGAATGAAAC CAAACTCAAA AATTTTGGGC AGGAATTAGC ACAAGTTCAG CACAGCATTG GGCAGCTTTG CAATACCAAG GATCTTCTTT TAGGAAAACT TGATATTATT TCACCCCAGC TGTCTTCTGC ATCATTGCTT ACTCCCCAGT CTGCAGAGTG TCTTAGAGCA AGTAAGTCTG AAGTATTGAG TGAATCTTCT GAATTGCTTC AGCAAGAGTT AGAAGAGCTA AGAAAATCAC TACAGGAAAA AGATGCAACA ATTAGAACTC TCCAGGAAAA TAACCACAGA TTGTCTGATT CGATTGCTGC CACCTCAGAG CTAGAAAGAA AAGAACACGA ACAAACCGAT TCAGAAATCA AGCAGCTAAA GGAGAAACAA GATGTTTTGC AAAAGTTACT TAAGGAAAAA GACCTCTTAA TCAAAGCCAA AAGTGATCAA CTACTTTCTT CCAATGAAAA TTTCACTAAC AAAGTAAATG AAAACGAACT TTTGAGGCAG GCAGTAACAA ACCTGAAGGA GAGAATATTA ATTCTAGAGA TGGACATTGG CAAACTAAAA GGAGAAAATG AAAAAATAGT GGAAACATAC AGGGGAAAGG AAACAGAATA TCAAGCGTTA CAAGAGACTA ACATGAAGTT TTCTATGATG CTGCGAGAAA AAGAGTTTGA GTGCCACTCA ATGAAGGAGA AGGCTCTTGC TTTTGAACAG CTATTGAAAG AGAAAGAACA GGGCAAGACT GGAGAGTTAA ATCAGCTTTT AAATGCAGTT AAATCAATGC AGGAGAAGAC AGTTGTGTTT CAACAGGAGA GAGACCAAGT CATGTTGGCC CTGAAACAAA AACAAATGGA AAATACTGCC CTACAGAATG AGGTTCAACG TTTACGTGAC AAAGAATTTC GTTCAAACCA AGAGCTAGAG AGATTGCGTA ATCATCTTTT AGAATCAGAA GATTCTTATA CCCGTGAAGC TTTGGCTGCA GAAGATAGAG AGGCTAAACT AAGAAAGAAA GTCACAGTAT TGGAGGAAAA GCTAGTTTCA TCCTCTAATG CAATGGAAAA TGCAAGCCAT CAAGCCAGTG TGCAGGTAGA GTCATTGCAA GAACAGTTGA ATGTAGTTTC CAAGCAAAGG GATGAAACTG CGCTGCAGCT TTCTGTCTCT CAGGAACAAG TAAAGCAGTA TGCTCTGTCA CTGGCCAACC TGCAGATGGT ACTAGAGCAT TTCCAACAAG AGGAAAAAGC TATGTATTCT GCTGAACTCG AAAAGCAAAA ACAGCTTATA GCTGAATGGA AGAAAAACGC AGAAAATCTG GAAGGAAAAG TGATATCATT ACAGGAATGT TTGGATGAAG CAAATGCTGC ATTGGATTCA GCATCAAGAC TTACAGAACA GTTAGATGTA AAAGAAGAAC AAATTGAAGA ACTTAAAAGA CAAAATGAGC TCCGACAAGA AATGCTGGAT GATGTACAAA AGAAATTGAT GAGCTTAGCA AACAGCTCAG AAGGAAAAGT AGACAAAGTC CTAATGAGAA ACCTCTTCAT TGGTCATTTC CACACACCGA AAAATCAGCG TCATGAAGTG TTACGGTTAA TGGGGAGCAT CCTGGGCGTC AGAAGGGAGG AGATGGAGCA GTTGTTTCAT GACGATCAGG GCGGTGTTAC CAGGTGGATG ACTGGGTGGC TTGGAGGAGG ATCAAAAAGT GTTCCCAACA CACCTTTGAG ACCAAATCAG CAATCTGTGG TTAATAGTTC TTTTTCAGAA CTTTTTGTTA AATTTCTAGA AACAGAATCT CATCCATCCA TTCCACCACC AAAGCTTTCT GTTCATGATA TGAAACCTCT GGATTCACCA GGAAGAAGAA AAAGAGATAC AAATGCACCA GAAAGTTTTA AAGATACAGC AGAATCCAGG TCTGGTAGAA GAACAGATGT AAATCCGTTT TTGGCTCCTC GCTCGGCAGC TGTACCTCTT ATTAACCCAG CTGGACTTGG ACCTGGTGGG CCCGGGCATC TTCTTCTGAA ACCCATCTCA GATGTTTTGC CCACATTTAC ACCTTTGCCA GCGTTACCTG ACAACAGTGC TGGGGTTGTG CTGAAAGACC TTTTAAAGCA ACTCGAGGGC GGTGGAGGCA GTGGTGGAGG CGGTAGCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAACGG GATCCGGACT AGTTAACGCG TCGACCAGTG GTCGACCCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TTACCATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGACGCCC GCCCCACGAC CCGCAGCGCC CGACCGAAAG GAGCGCACGA CCCCATGCAT CGCCAGTCGA CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGAATTCG AGCTCGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA TCTGCTTTTT GCTTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTAGTAGTTC ATGTCATCTT ATTATTCAGT ATTTATAACT TGCAAAGAAA TGAATATCAG AGAGTGAGAG GAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGCTCTAGC TATCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGGACGTACC CAATTCGCCC TATAGTGAGT CGTATTACGC GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAG
