pCMV-3×FLAG-MAP2(human)-EGFP-Neo
$128.00
In stock
SKU
PL41097
NM_002374.4;MAP-2; MAP2A; MAP2B; MAP2C
100μg liquid plasmid(Instantly):$149
| name | pCMV-3×FLAG-MAP2(human)-EGFP-Neo | another name | apCMV-3×FLAG-MAP2(human)-EGFP-Neo |
| Insert Size (bp) | 5484bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGACTCAG ATCTCGAGCT CCGAATTCGC CACCATGGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGAAGCTTA TGGCAGATGA ACGGAAAGAT GAAGCAAAGG CACCTCACTG GACCTCAGCA CCGCTAACAG AGGCATCTGC ACACTCACAT CCACCTGAGA TTAAGGATCA AGGCGGAGCA GGGGAAGGAC TTGTCCGAAG CGCCAATGGA TTCCCATACA GGGAGGATGA AGAGGGTGCC TTTGGAGAGC ATGGGTCACA GGGCACCTAT TCAAATACCA AAGAGAATGG GATCAACGGA GAGCTGACCT CAGCTGACAG AGAAACAGCA GAGGAGGTGT CTGCAAGGAT AGTTCAAGTA GTCACTGCTG AGGCTGTAGC AGTCCTGAAA GGTGAACAAG AGAAAGAAGC TCAACATAAA GACCAGACTG CAGCTCTGCC TTTAGCAGCT GAAGAAACAG CTAATCTGCC TCCTTCTCCA CCCCCATCAC CTGCCTCAGA ACAGACTGTC ACAGTGGAGG AAGATTTACT TACAGCCTCG AAGATGGAGT TCCACGATCA ACAGGAATTG ACTCCCTCTA CAGCTGAGCC TTCAGACCAG AAGGAAAAGG AGTCAGAGAA GCAAAGTAAG CCTGGTGAAG ACCTTAAACA TGCTGCCTTA GTTTCTCAGC CAGAGACAAC TAAAACTTAC CCTGATAAAA AGGACATGCA AGGCACGGAA GAAGAAAAAG CACCCCTAGC TTTGTTTGGG CACACTCTTG TTGCCAGCCT GGAAGACATG AAACAGAAGA CAGAACCAAG CCTTGTAGTA CCTGGCATTG ACCTCCCTAA AGAGCCTCCA ACTCCAAAAG AACAAAAGGA CTGGTTCATC GAAATGCCAA CGGAAGCAAA AAAGGATGAG TGGGGTTTAG TTGCCCCCAT ATCTCCTGGC CCTCTGACTC CCATGAGGGA AAAAGATGTA TTTGATGATA TCCCAAAATG GGAAGGGAAA CAGTTTGATT CTCCCATGCC AAGTCCCTTT CAAGGGGGAA GCTTCACTCT TCCTTTAGAT GTCATGAAGA ATGAAATAGT TACAGAAACA TCGCCCTTTG CCCCTGCCTT TTTACAGCCA GATGACAAAA AATCTCTGCA ACAAACCAGT GGCCCAGCTA CTGCCAAAGA TAGTTTTAAA ATTGAAGAGC CCCATGAGGC TAAACCTGAC AAAATGGCAG AAGCACCACC CTCAGAGGCA ATGACCTTAC CCAAAGATGC TCACATTCCA GTTGTAGAAG AACATGTTAT GGGGAAAGTT TTAGAGGAAG AAAAGGAGGC CATAAATCAA GAGACTGTGC AGCAAAGGGA TACTTTCACC CCCAGTGGAC AGGAACCTAT ACTTACTGAA AAGGAAACTG AGCTGAAGCT TGAAGAAAAA ACCACCATTT CTGACAAAGA AGCTGTGCCA AAAGAGAGTA AACCCCCAAA ACCTGCAGAT GAAGAAATAG GCATAATTCA GACCTCCACA GAGCACACTT TCTCAGAACA GAAAGACCAA GAGCCTACCA CAGATATGTT GAAACAGGAC TCGTTCCCTG TAAGTTTGGA GCAAGCAGTT ACAGATTCAG CCATGACCTC TAAAACACTG GAGAAAGCCA TGACCGAACC ATCTGCATTA ATTGAAAAGA GCTCAATTCA GGAACTTTTT GAAATGAGAG TTGATGACAA AGATAAGATT GAAGGAGTTG GAGCTGCAAC ATCAGCTGAG CTTGATATGC CATTTTATGA AGATAAATCA GGAATGTCCA AGTACTTTGA AACATCTGCC TTGAAAGAAG AAGCAACAAA AAGCATTGAG CCAGGCAGTG ATTACTATGA ACTGAGTGAC ACTAGAGAAA GTGTCCATGA GTCTATTGAT ACCATGTCTC CCATGCATAA AAATGGTGAC AAGGAGTTTC AAACAGGAAA AGAATCCCAG CCCAGTCCTC CAGCACAAGA AGCAGGGTAC AGCACTCTCG CACAGAGTTA TCCATCAGAT TTACCTGAAG AACCCAGTTC TCCTCAAGAA AGAATGTTCA CTATTGATCC AAAAGTGTAT GGAGAGAAAA GGGACCTCCA CAGTAAGAAT AAGGATGATT TGACCCTTAG CAGGAGTTTA GGACTTGGTG GTAGGTCTGC AATAGAACAA AGAAGCATGT CAATCAATTT GCCGATGTCT TGCCTAGATT CCATAGCCCT TGGATTTAAC TTTGGTCGGG GACATGATCT TTCTCCTCTG GCTTCCGATA TTCTAACCAA CACTAGTGGA AGTATGGATG AAGGGGATGA TTACCTTCCA GCCACCACAC CTGCACTGGA GAAAGCCCCT TGCTTCCCTG TAGAAAGCAA AGAGGAAGAA CAGATAGAGA AAGTAAAAGC TACTGGAGAA GAAAGTACTC AAGCGGAGAT ATCATGTGAG TCTCCTTTCC TAGCCAAAGA TTTTTACAAA AATGGTACTG TCATGGCACC TGACCTTCCT GAAATGCTAG ATCTGGCAGG CACAAGGTCA AGATTGGCTT CTGTGAGTGC AGATGCTGAG GTTGCCAGGA GGAAATCAGT CCCATCAGAG ACTGTGGTTG AGGATAGTCG TACTGGCTTG CCCCCGGTAA CTGATGAAAA CCATGTCATT GTAAAAACGG ACAGTCAGCT CGAAGACCTG GGCTACTGTG TGTTCAATAA GTACACAGTC CCATTGCCAT CACCTGTTCA AGACAGTGAG AATTTATCAG GGGAGAGTGG TACCTTTTAC GAAGGCACTG ATGATAAAGT TCGAAGAGAT TTGGCCACAG ACCTTTCACT GATTGAAGTG AAACTGGCAG CAGCCGGAAG AGTCAAAGAT GAGTTCAGTG TTGACAAAGA AGCATCCGCG CATATCTCTG GTGACAAATC AGGACTGAGT AAGGAGTTTG ACCAAGAGAA GAAAGCTAAT GATAGGTTGG ATACTGTACT AGAAAAGAGT GAAGAACATG CTGATTCAAA AGAACATGCC AAGAAAACTG AAGAGGCTGG TGATGAAATA GAAACATTCG GATTAGGAGT AACCTATGAG CAAGCTTTGG CCAAAGATTT GTCAATACCA ACAGATGCAT CCTCTGAGAA AGCAGAGAAG GGTCTTAGTT CAGTGCCAGA GATAGCTGAG GTAGAACCAT CCAAAAAGGT GGAACAAGGT CTGGATTTTG CTGTCCAGGG TCAACTAGAT GTTAAAATTA GTGACTTTGG ACAGATGGCT TCAGGGCTAA ACATAGATGA TAGAAGGGCA ACAGAGCTAA AACTTGAGGC TACACAGGAC ATGACCCCCT CATCCAAAGC ACCGCAGGAG GCAGATGCAT TTATGGGTGT TGAGTCTGGC CACATGAAAG AAGGCACTAA AGTTAGTGAG ACAGAAGTCA AAGAGAAGGT GGCCAAGCCT GACTTGGTGC ACCAGGAGGC TGTAGACAAG GAGGAGTCCT ATGAATCTAG TGGTGAGCAT GAAAGTCTCA CCATGGAGTC CTTGAAAGCT GATGAGGGCA AGAAGGAAAC ATCTCCAGAA TCATCTCTAA TTCAAGATGA GATTGCCGTC AAATTGTCAG TGGAAATACC TTGCCCACCT GCTGTTTCAG AGGCTGATTT AGCCACAGAT GAGAGAGCTG ATGTCCAGAT GGAATTTATT CAGGGGCCAA AAGAAGAAAG CAAAGAGACC CCAGATATAT CCATCACGCC TTCTGATGTT GCAGAGCCAT TGCATGAAAC GATCGTATCT GAACCAGCAG AGATTCAGAG TGAGGAAGAA GAGATAGAAG CCCAGGGAGA ATATGATAAA CTGCTCTTCC GCTCAGACAC CCTTCAGATA ACTGACCTGG GTGTCTCAGG TGCCAGGGAG GAATTTGTGG AGACCTGCCC AAGTGAACAC AAAGGAGTGA TTGAGTCTGT TGTGACCATC GAGGATGATT TCATCACTGT AGTGCAAACC ACAACTGATG AAGGGGAGTC AGGGTCCCAC AGCGTGCGTT TTGCAGCCCT AGAGCAGCCT GAGGTGGAAA GGAGACCATC TCCTCATGAT GAAGAAGAGT TTGAAGTAGA AGAGGCAGCT GAAGCCCAGG CAGAACCCAA AGATGGTTCC CCAGAGGCTC CAGCTTCCCC TGAGAGAGAA GAGGTTGCAC TTTCTGAATA TAAGACAGAA ACCTATGACG ATTACAAAGA TGAGACCACC ATTGACGACT CCATCATGGA CGCTGACAGC CTCTGGGTGG ACACTCAAGA TGATGATAGG AGCATCATGA CAGAACAGTT AGAAACTATT CCTAAAGAGG AGAAAGCTGA AAAGGAAGCT CGGAGATCAT CTCTTGAGAA ACATAGAAAA GAAAAGCCTT TTAAAACCGG GAGAGGCAGA ATTTCCACTC CTGAAAGAAA AGTAGCTAAA AAGGAACCTA GCACAGTCTC CAGAGATGAA GTGAGAAGGA AAAAAGCAGT TTATAAGAAG GCTGAACTTG CTAAAAAAAC AGAAGTTCAG GCCCACTCTC CCTCCAGGAA ATTCATTTTA AAACCTGCTA TCAAATATAC TAGACCAACT CATCTCTCCT GTGTTAAGCG GAAAACCACA GCAGCAGGTG GGGAATCAGC TCTGGCTCCC AGTGTATTTA AACAGGCAAA GGACAAAGTC TCTGACGGAG TAACCAAGAG CCCAGAAAAG CGCTCTTCTC TCCCAAGACC TTCCTCCATT CTCCCTCCTC GGCGAGGTGT GTCAGGAGAC AGAGATGAGA ATTCCTTCTC TCTCAACAGT TCTATCTCTT CTTCAGCACG GCGGACCACC AGGTCAGAGC CAATTCGCAG AGCAGGGAAG AGTGGTACCT CAACACCCAC TACCCCTGGG TCTACTGCCA TCACTCCTGG CACCCCACCA AGTTATTCTT CACGCACACC AGGCACTCCT GGAACCCCTA GCTATCCCAG GACCCCTCAC ACACCAGGAA CCCCCAAGTC TGCCATCTTG GTGCCGAGTG AGAAGAAGGT CGCCATCATA CGTACTCCTC CAAAATCTCC TGCGACTCCC AAGCAGCTTC GGCTTATTAA CCAACCACTG CCAGACCTGA AGAATGTCAA ATCCAAAATC GGATCAACAG ACAACATCAA ATACCAGCCT AAAGGGGGGC AGGTACAAAT TGTTACCAAG AAAATAGACC TAAGCCATGT GACATCCAAA TGTGGCTCTC TGAAGAACAT CCGCCACAGG CCAGGTGGCG GACGTGTGAA AATTGAGAGT GTAAAACTAG ATTTCAAAGA AAAGGCCCAA GCTAAAGTTG GTTCTCTTGA TAATGCTCAT CATGTACCTG GAGGTGGTAA TGTCAAGATT GACAGCCAAA AGTTGAACTT CAGAGAGCAT GCTAAAGCCC GTGTGGACCA TGGGGCTGAG ATCATTACAC AGTCCCCAGG CAGATCCAGC GTGGCATCAC CCCGACGACT CAGCAATGTC TCCTCGTCTG GAAGCATCAA CCTGCTCGAA TCTCCTCAGC TTGCCACTTT GGCTGAGGAT GTCACTGCTG CACTCGCTAA GCAGGGCTTG TGAGGATCCG GGCCCGTTTA AACCCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC TATGGTCTAG AGATATACTG AGTCATTAGG GACTTTCCAA TGGGTTTTGC CCAGTACATA AGGTCAATAG GGGTGAATCA ACAGGAAAGT CCCATTGGAG CCAAGTACAC TGAGTCAATA GGGACTTTCC ATTGGGTTTT GCCCAGTACA AAAGGTCAAT AGGGGGTGAG TCAATGGGTT TTTCCCATTA TTGGCACGTA CATAAGGTCA ATAGGGGTGA GTCATTGGGT TTTTCCAGCC AATTTAATTA AAACGCCATG TACTTTCCCA CCATTGACGT CAATGGGCTA TTGAAACTAA TGCAACGTGA CCTTTAAACG GTACTTTCCC ATAGCTGATT AATGGGAAAG TACCGTTCTC GAGCCAATAC ACGTCAATGG GAAGTGAAAG GGCAGCCAAA ACGTAACACC GCCCCGGTTT TCCCCTGGAA ATTCCATATT GGCACGCATT CTATTGGCTG AGCTGCGTTC TACGTGGGTA TAAGAGGCGC GACCAGCGTC GGTACCGTCG CAGTCTTCGG TCTGACCACC GTAGAACGCA GATCACCGGT CGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGC GGCCGCGACT CTAGATCATA ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT AAGGCGTAAA TTGTAAGCGT TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTCCTGAG GCGGAAAGAA CCAGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAGATCGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCTAGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGTGTTGG GTCGTTTGTT CATAAACGCG GGGTTCGGTC CCAGGGCTGG CACTCTGTCG ATACCCCACC GAGACCCCAT TGGGGCCAAT ACGCCCGCGT TTCTTCCTTT TCCCCACCCC ACCCCCCAAG TTCGGGTGAA GGCCCAGGGC TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG CCATAGCCTC AGGTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCATGC AT
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