pTol2-DDX4(zebrafish)-Promoter-2A-EGFP-Neo
$128.00
In stock
SKU
PL41068
100μg liquid plasmid(Instantly):$149
| name | pTol2-DDX4(zebrafish)-Promoter-2A-EGFP-Neo | another name | apTol2-DDX4(zebrafish)-Promoter-2A-EGFP-Neo |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Transpose,Signal report | Fragment type | Promoter |
| Fragment species | Zebrafish | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | DDX4 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGAGGTGTA AAGTACTTGA GTAATTTTAC TTGATTACTG TACTTAAGTA TTATTTTTGG GGATTTTTAC TTTACTTGAG TACAATTAAA AATCAATACT TTTACTTTTA CTTAATTACA TTTTTTTAGA AAAAAAAGTA CTTTTTACTC CTTACAATTT TATTTACAGT CAAAAAGTAC TTATTTTTTG GAGATCACTT CATTCTATTT TCCCTTGCTA TTAGCTAGCG CTACCGGACT CAGATCTCGA GAAGCACCCG AAGGAAACCT TCACAAGCAC GGAGAAACAT GCAACCTCTA CACAGAAATG CTTACTGACC CAGCCGGGCC TCGAACCAGC AACTTTCTTG CTGTATAGGC GATCAAACAA CCCACTGCGC CATGATGCCC CAATATCAGT GTTAATTCTC AGTGCTATTA TCGTGCAGTA TTTTTTACCC CTTCATGCAG TGCAGCATGA TGTCTCCATT TTCAGCAGGT TTTCTCTCAC TTAGATTATA TATTTTTTGG AATTATACAT TACTTAGTCT CATGTATCTC TTTTTTTCTT TTAAGCATCT TAAACAGAAA CTCTGCAGTT TTGTTGTATC TCGTATAAAA TGACAATAAA GACTTTTATT CTAATATTTC ACAACAGATT TAAGGATATA TATGGATAGT TACAGTTGAA GTCAGAATTA TTAGCCCCCC TTTCTTTTTT TTCTGTTTTA AATATTTCCC AAATTATGTT TAAAAGAGCA AGGAAATTTT CACAGTATGT CTGATAATAT TTTTTCTTTT GGAGAAGTTT TATTTCGGCA AGAATTGAAA CAGTTTTTAC TTTTTTAAAC ACCATTTTAA GGTCAATATT ATTAGCCCCT TTAAGCTTAT TTTCCTTAAA AGTTTACACA ACAAATCATT GTTATACAAT AACTTACCTA ATTACCCTAA CCTGCCTAAC TTGCCTAATT ACCCTAAGCC TTTAAATGTC ACTTTAAGCT GTATAGAAGT GTCTTGAAGA GTATCTGGTA AAATATTATT TACTATCATC ATGGCAAAGA TAAAATAAAT CAGTTATTAG AAATGAATTA TGAAAACTGT TATGAAATGT GTTGAAAAAA TCTGCTCTCC GTTTAACAGA AATTGGGGAA AAAATAAACA GGGCTTATAA TTCCCGAGGC TAATAATTCT GACTTCAACT ATGTGTACAT ATATACATTT TTTTTACTTT GTTTCATTTC TTTGCGAGTT TTAGATGCAA TGGTAAAAGG TGCATTTTCA TATACACTTA AAACACATGA AATTGATCAT TTATAATTAA AAAAAAACTT TCTTAGATTT AGTTTATAGC TTAATATTTG CACTGTACCA TTCATTGAGG ACCCATGTGT TGCATATAGT TTTTTACACT GGATATCTTT ACTCAGATCT CATGGGCACA AACTTGATAG TCTGATCAAC AGTCATTTAG AAAGGACAAA CACCTTGCAT TAAACAACAG TTATGTTTTG CCACCAAACA GTGTTATTAT TATGTAAGCA TGAGAGTATT GTGAATACAG TCACATCTCA AGCTTGATAT ACATGGTTAC CACACAGTTA TAAAAAATAT TACCAAAACA GATTGTTTAA CATAGTAACA AATAAATTTT GGCTGAAATG GGTTATATAA CTTTTTTATC AGAGTGACAT GCGGCGCAGT AGGTAGCACG ATCGCCTCAC AGAAAGGTCA CTTGTTCGAG CCCCGGCTGG GTCAGTTGGC ATTTCTGTGT GGAGTTTGCA TGTCCTCCCC GTGTTGGCGT AGGTTTCCTC TGGGTGCTCC AAAGACATGT GTTATTATAG GTGAATTGAA TAAGATAAAT TGACCGTAGT GCATGTATGT AAATGCAAGG GTGTGTTCCA GTGCTGGTAG GGCATCTACT GCGTAAAACA TATGCTGGAT AAGTTGGTGG TTCATTCCGC TGTGGTGACC CCTGATTAAT AAAGAGACTC AGCTAAATAT AAATGAATGA ATGAACTTGT TTTATTTTAC AATTGTTTTT ATAATTAAAT ATTGGAGATC CTAACCATCC ATACACGTGA AAATACAAAT AAAAATCAAT TCAACTGGGA ATTTTAGCAG TATACTGACA GTTTTATAAA CATTTCAGTT TCATTATACC TTTTTTGCTT GAAGAATATT TAAATCAACA AAATACTTAT TAAACGCTAA AAGTCCTCCT TGAGATCGGT TCACTAGTTC ATTTCGATTT AGACATGACC GGAAGAGTTG TGACTCGGTA TAATTGATTC AGACCAATGA GGTTTCAAAT CAAACGCGAT CAAACGACGG ATTCAAACGG GTCTGCGAAT CACTCGCTTG ATTCATTTAA AAAAAAGAAT CTGTTCAAAA CGCGAATCGG ACGTTACATC GATTTTCCCG CCCACGAGCA AAACGATGAC GTCATAGCTG GCCATCTTTC TTTCTCTCGC GGAATTCTGC AGTCGACGGT ACCGCGGGCC CGGGATCCGG AGGTGGCAGC GGTGGCGGTC AGCTGTTGAA TTTTGACCTT CTTAAACTTG CGGGAGACGT CGAGTCCAAC CCTGGGCCCA CAACCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGCGACTCT AGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA GGCTTAAACA AGAATCTCTA GTTTTCTTTC TTGCTTTTAC TTTTACTTCC TTAATACTCA AGTACAATTT TAATGGAGTA CTTTTTTACT TTTACTCAAG TAAGATTCTA GCCAGATACT TTTACTTTTA ATTGAGTAAA ATTTTCCCTA AGTACTTGTA CTTTCACTTG AGTAAAATTT TTGAGTACTT TTTACACCTC TGCTTAAGGC GTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTC CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCT AGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGT GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA GCCTCAGGTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CATGCAT
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