pTRE3G-BXI1(yeast)-EGFP-3×FLAG-Tetone-Puro
$84.00
In stock
SKU
PL41065
NM_001183143.1;YBH3
100μg liquid plasmid(Instantly):$105
| name | pTRE3G-BXI1(yeast)-EGFP-3×FLAG-Tetone-Puro | another name | apTRE3G-BXI1(yeast)-EGFP-3×FLAG-Tetone-Puro |
| Insert Size (bp) | 894bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Yeast | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | TRE3G | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGTCGCCGCT TGGAGCTCCC GTGAGGCGTG CTTGTCAATG CGGTAAGTGT CACTGATTTT GAACTATAAC GACCGCGTGA GTCAAAATGA CGCATGATTA TCTTTTACGT GACTTTTAAG ATTTAACTCA TACGATAATT ATATTGTTAT TTCATGTTCT ACTTACGTGA TAACTTATTA TATATATATT TTCTTGTTAT AGATATCATA ACTTCGTATA ATGTATGCTA TACGAAGTTA TGCGGCCCTC GAGTTTACTC CCTATCAGTG ATAGAGAACG TATGAAGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG CAGACTTTAC TCCCTATCAG TGATAGAGAA CGTATAAGGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGACCAGTTT ACTCCCTATC AGTGATAGAG AACGTATCTA CAGTTTACTC CCTATCAGTG ATAGAGAACG TATATCCAGT TTACTCCCTA TCAGTGATAG AGAACGTATA AGCTTTAGGC GTGTACGGTG GGCGCCTATA AAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG CAATTCCACA ACACTTTTGT CTTATACCAA CTTTCCGTAC CACTTCCTAC CCTCGTAAAG TCGACACCGG GGCCCAGATC TATCGATCGG CCGGATATCA CGCGTCATAT GGCTAGCCTG CAGGGATCCG CCACCATGTC AGGTCCTCCA CCTCCTTACG AAGAGCAAAG CTCACATCTT TACGGCCAGC CTGCGAGCAG CCAGGATGGA AACGCTTTCA TCCCGGAAGA CTTTAAATAT TCCACGGTGG TTATCTCGTG CGAACCCATC ATTCGCCAGC GGTTTATGCA CAAGGTTTAC TCTCTGCTGT CATGCCAGTT ACTGGCCAGT TTGTCGTTCT GCTACTGGGC TAGCGTTTCC ACTTCTTTGC AGAACTTTAT CATGTCGCAT ATCGCCCTCT TCTACATCTG CATGGTGGTA TCGCTAGTAT CGTGCATTTG GCTGGCAGTA AGTCCTCGTC CCGAAGACTA CGAGGCCAGT GTTCCCGAAC CGTTGCTCAC GGGCAGCAGC GAAGAGCCGG CACAAGAGCA AAGGCGTCTG CCTTGGTACG TCTTGTCCTC GTACAAGCAA AAGCTCACGC TGCTCTCCAT TTTCACTCTT TCCGAGGCTT ACTGTCTGTC GCTGGTAACC TTAGCGTACG ATAAGGATAC AGTACTTTCG GCTCTGCTGA TCACCACCAT CGTCGTAGTC GGCGTTTCCT TGACCGCACT ATCAGAGAGA TTCGAGAACG TGCTGAATTC CGCAACGTCA ATATACTATT GGTTAAACTG GGGTCTATGG ATTATGATCG GAATGGGTCT CACGGCCTTG CTTTTCGGCT GGAATACCCA CTCTTCCAAG TTTAATCTGC TTTACGGCTG GCTTGGTGCC ATTTTATTTA CCGCGTATCT TTTCATCGAT ACGCAACTGA TTTTTAGGAA AGTGTATCCT GATGAAGAAG TTAGATGCGC CATGATGCTT TACCTGGACA TCGTCAATTT GTTTTTGTCT ATTTTAAGGA TTTTGGCCAA CTCTAACGAC GACAATGAAT TCGCCACCAA CTTCAGCCTG CTGAAGCAGG CCGGCGACGT GGAGGAGAAC CCCGGCCCCA TGGTGTCTAA GGGCGAAGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGGTCG ACGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGTA GTCTAGCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGACTAGAGC GATCCCTAGG CTAGGCTTTT GCAAAGCTAG CAGCTGAATC TAAGTGGATC TGCGATCGCT CCGGTGCCCG TCAGTGGGCA GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA TTGAACGGGT GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG GCTCCGCCTT TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA CGTTCTTTTT CGCAACGGGT TTGCCGCCAG AACACAGCTG AAGCATCGAG GGGCTCGCAT CTCTCCTTCA CGCGCCCGCC GCCCTACCTG AGGCCGCCAT CCACGCCGGT TGAGTCGCGT TCTGCCGCCT CCCGCCTGTG GTGCCTCCTG AACTGCGTCC GCCGTCTAGG TAAGTTTAAA GCTCAGGTCG AGACCGGGCC TTTGTCCGGC GCTCCCTTGG AGCCTACCTA GACTCAGCCG GCTCTCCACG CTTTGCCTGA CCCTGCTTGC TCAACTCTAC GTCTTTGTTT CGTTTTCTGT TCTGCGCCGT TACAGATCCA AGCTGTGACC GGCGCCTACT CTAGAGCCGC CATGTCTAGA CTGGACAAGA GCAAAGTCAT AAACTCTGCT CTGGAATTAC TCAATGGAGT CGGTATCGAA GGCCTGACGA CAAGGAAACT CGCTCAAAAG CTGGGAGTTG AGCAGCCTAC CCTGTACTGG CACGTGAAGA ACAAGCGGGC CCTGCTCGAT GCCCTGCCAA TCGAGATGCT GGACAGGCAT CATACCCACT CCTGCCCCCT GGAAGGCGAG TCATGGCAAG ACTTTCTGCG GAACAACGCC AAGTCATACC GCTGTGCTCT CCTCTCACAT CGCGACGGGG CTAAAGTGCA TCTCGGCACC CGCCCAACAG AGAAACAGTA CGAAACCCTG GAAAATCAGC TCGCGTTCCT GTGTCAGCAA GGCTTCTCCC TGGAGAACGC ACTGTACGCT CTGTCCGCCG TGGGCCACTT TACACTGGGC TGCGTATTGG AGGAACAGGA GCATCAAGTA GCAAAAGAGG AAAGAGAGAC ACCTACCACC GATTCTATGC CCCCACTTCT GAAACAAGCA ATTGAGCTGT TCGACCGGCA GGGAGCCGAA CCTGCCTTCC TTTTCGGCCT GGAACTAATC ATATGTGGCC TGGAGAAACA GCTAAAGTGC GAAAGCGGCG GGCCGACCGA CGCCCTTGAC GATTTTGACT TAGACATGCT CCCAGCCGAT GCCCTTGACG ACTTTGACCT TGATATGCTG TCTGCTGACG CTCTTGACGA TTTTGACCTT GACATGCTCC CCGGGGAGGG CAGAGGAAGT CTTCTAACAT GCGGTGACGT GGAGGAGAAT CCCGGCCCTC TTAAGATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGAGCGGC CGCGACTCTA GTCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTAGGTA CAAGTAGGCG CGTCGAGATA ACTTCGTATA ATGTATGCTA TACGAAGTTA TGTTTTGTTA CTTTATAGAA GAAATTTTGA GTTTTTGTTT TTTTTTAATA AATAAATAAA CATAAATAAA TTGTTTGTTG AATTTATTAT TAGTATGTAA GTGTAAATAT AATAAAACTT AATATCTATT CAAATTAATA AATAAACGTC AATTTTACGC ATGATTATCT TTAACGTACG TCACAATATG ATTATCTTTC TAGGGTTAAA GAGCTCCAAT TCGCCCTATA GTGAGTCGTA TTACGCGCGC TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC AAGCGCGCAA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG GTACCTTAAC CCTAGAAAGA TAGTCTGCGT AAAATTGACG CATGCATTCT TGAAATATTG CTCTCTCTTT CTAAATAGCG CGAATCCGTC GCTGTGCATT TAGGACATCT 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