pEF1a-CFH(human)-FLAG-Neo
100μg liquid plasmid(Instantly):$120
100μg liquid plasmid(Instantly):$120
| name | pEF1a-CFH(human)-FLAG-Neo | another name | apEF1a-CFH(human)-FLAG-Neo |
| Insert Size (bp) | 3693bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | EF1a | Replicon | pUC |
| Competence | BL21(DE3) | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTGG CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACCG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGG TAAGTGCCGT GTGTGGTTCC CGCGGGCCTG GCCTCTTTAC GGGTTATGGC CCTTGCGTGC CTTGAATTAC TTCCACCTGG CTGCAGTACG TGATTCTTGA TCCCGAGCTT CGGGTTGGAA GTGGGTGGGA GAGTTCGAGG CCTTGCGCTT AAGGAGCCCC TTCGCCTCGT GCTTGAGTTG AGGCCTGGCC TGGGCGCTGG GGCCGCCGCG TGCGAATCTG GTGGCACCTT CGCGCCTGTC TCGCTGCTTT CGATAAGTCT CTAGCCATTT AAAATTTTTG ATGACCTGCT GCGACGCTTT TTTTCTGGCA AGATAGTCTT GTAAATGCGG GCCAAGATCT GCACACTGGT ATTTCGGTTT TTGGGGCCGC GGGCGGCGAC GGGGCCCGTG CGTCCCAGCG CACATGTTCG GCGAGGCGGG GCCTGCGAGC GCGGCCACCG AGAATCGGAC GGGGGTAGTC TCAAGCTGGC CGGCCTGCTC TGGTGCCTGG CCTCGCGCCG CCGTGTATCG CCCCGCCCTG GGCGGCAAGG CTGGCCCGGT CGGCACCAGT TGCGTGAGCG GAAAGATGGC CGCTTCCCGG CCCTGCTGCA GGGAGCTCAA AATGGAGGAC GCGGCGCTCG GGAGAGCGGG CGGGTGAGTC ACCCACACAA AGGAAAAGGG CCTTTCCGTC CTCAGCCGTC GCTTCATGTG ACTCCACGGA GTACCGGGCG CCGTCCAGGC ACCTCGATTA GTTCTCGAGC TTTTGGAGTA CGTCGTCTTT AGGTTGGGGG GAGGGGTTTT ATGCGATGGA GTTTCCCCAC ACTGAGTGGG TGGAGACTGA AGTTAGGCCA GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGA ATTAGCTTGG TACGCTAGCG TTTAAACTTA AGCTTGGTAC CGAGCTCGGA TCCGCCACCA TGAGACTTCT AGCAAAGATT ATTTGCCTTA TGTTATGGGC TATTTGTGTA GCAGAAGATT GCAATGAACT TCCTCCAAGA AGAAATACAG AAATTCTGAC AGGTTCCTGG TCTGACCAAA CATATCCAGA AGGCACCCAG GCTATCTATA AATGCCGCCC TGGATATAGA TCTCTTGGAA ATGTAATAAT GGTATGCAGG AAGGGAGAAT GGGTTGCTCT TAATCCATTA AGGAAATGTC AGAAAAGGCC CTGTGGACAT CCTGGAGATA CTCCTTTTGG TACTTTTACC CTTACAGGAG GAAATGTGTT TGAATATGGT GTAAAAGCTG TGTATACATG TAATGAGGGG TATCAATTGC TAGGTGAGAT TAATTACCGT GAATGTGACA CAGATGGATG GACCAATGAT ATTCCTATAT GTGAAGTTGT GAAGTGTTTA CCAGTGACAG CACCAGAGAA TGGAAAAATT GTCAGTAGTG CAATGGAACC AGATCGGGAA TACCATTTTG GACAAGCAGT ACGGTTTGTA TGTAACTCAG GCTACAAGAT TGAAGGAGAT GAAGAAATGC ATTGTTCAGA CGATGGTTTT TGGAGTAAAG AGAAACCAAA GTGTGTGGAA ATTTCATGCA AATCCCCAGA TGTTATAAAT GGATCTCCTA TATCTCAGAA GATTATTTAT AAGGAGAATG AACGATTTCA ATATAAATGT AACATGGGTT ATGAATACAG TGAAAGAGGA GATGCTGTAT GCACTGAATC TGGATGGCGT CCGTTGCCTT CATGTGAAGA AAAATCATGT GATAATCCTT ATATTCCAAA TGGTGACTAC TCACCTTTAA GGATTAAACA CAGAACTGGA GATGAAATCA CGTACCAGTG TAGAAATGGT TTTTATCCTG CAACCCGGGG AAATACAGCA AAATGCACAA GTACTGGCTG GATACCTGCT CCGAGATGTA CCTTGAAACC TTGTGATTAT CCAGACATTA AACATGGAGG TCTATATCAT GAGAATATGC GTAGACCATA CTTTCCAGTA GCTGTAGGAA AATATTACTC CTATTACTGT GATGAACATT TTGAGACTCC GTCAGGAAGT TACTGGGATC ACATTCATTG CACACAAGAT GGATGGTCGC CAGCAGTACC ATGCCTCAGA AAATGTTATT TTCCTTATTT GGAAAATGGA TATAATCAAA ATCATGGAAG AAAGTTTGTA CAGGGTAAAT CTATAGACGT TGCCTGCCAT CCTGGCTACG CTCTTCCAAA AGCGCAGACC ACAGTTACAT GTATGGAGAA TGGCTGGTCT CCTACTCCCA GATGCATCCG TGTCAAAACA TGTTCCAAAT CAAGTATAGA TATTGAGAAT GGGTTTATTT CTGAATCTCA GTATACATAT GCCTTAAAAG AAAAAGCGAA ATATCAATGC AAACTAGGAT ATGTAACAGC AGATGGTGAA ACATCAGGAT CAATTACATG TGGGAAAGAT GGATGGTCAG CTCAACCCAC GTGCATTAAA TCTTGTGATA TCCCAGTATT TATGAATGCC AGAACTAAAA ATGACTTCAC ATGGTTTAAG CTGAATGACA CATTGGACTA TGAATGCCAT GATGGTTATG AAAGCAATAC TGGAAGCACC ACTGGTTCCA TAGTGTGTGG TTACAATGGT TGGTCTGATT TACCCATATG TTATGAAAGA GAATGCGAAC TTCCTAAAAT AGATGTACAC TTAGTTCCTG ATCGCAAGAA AGACCAGTAT AAAGTTGGAG AGGTGTTGAA ATTCTCCTGC AAACCAGGAT TTACAATAGT TGGACCTAAT TCCGTTCAGT GCTACCACTT TGGATTGTCT CCTGACCTCC CAATATGTAA AGAGCAAGTA CAATCATGTG GTCCACCTCC TGAACTCCTC AATGGGAATG TTAAGGAAAA AACGAAAGAA GAATATGGAC ACAGTGAAGT GGTGGAATAT TATTGCAATC CTAGATTTCT AATGAAGGGA CCTAATAAAA TTCAATGTGT TGATGGAGAG TGGACAACTT TACCAGTGTG TATTGTGGAG GAGAGTACCT GTGGAGATAT ACCTGAACTT GAACATGGCT GGGCCCAGCT TTCTTCCCCT CCTTATTACT ATGGAGATTC AGTGGAATTC AATTGCTCAG AATCATTTAC AATGATTGGA CACAGATCAA TTACGTGTAT TCATGGAGTA TGGACCCAAC TTCCCCAGTG TGTGGCAATA GATAAACTTA AGAAGTGCAA ATCATCAAAT TTAATTATAC TTGAGGAACA TTTAAAAAAC AAGAAGGAAT TCGATCATAA TTCTAACATA AGGTACAGAT GTAGAGGAAA AGAAGGATGG ATACACACAG TCTGCATAAA TGGAAGATGG GATCCAGAAG TGAACTGCTC AATGGCACAA ATACAATTAT GCCCACCTCC ACCTCAGATT CCCAATTCTC ACAATATGAC AACCACACTG AATTATCGGG ATGGAGAAAA AGTATCTGTT CTTTGCCAAG AAAATTATCT AATTCAGGAA GGAGAAGAAA TTACATGCAA AGATGGAAGA TGGCAGTCAA TACCACTCTG TGTTGAAAAA ATTCCATGTT CACAACCACC TCAGATAGAA CACGGAACCA TTAATTCATC CAGGTCTTCA CAAGAAAGTT ATGCACATGG GACTAAATTG AGTTATACTT GTGAGGGTGG TTTCAGGATA TCTGAAGAAA ATGAAACAAC ATGCTACATG GGAAAATGGA GTTCTCCACC TCAGTGTGAA GGCCTTCCTT GTAAATCTCC ACCTGAGATT TCTCATGGTG TTGTAGCTCA CATGTCAGAC AGTTATCAGT ATGGAGAAGA AGTTACGTAC AAATGTTTTG AAGGTTTTGG AATTGATGGG CCTGCAATTG CAAAATGCTT AGGAGAAAAA TGGTCTCACC CTCCATCATG CATAAAAACA GATTGTCTCA GTTTACCTAG CTTTGAAAAT GCCATACCCA TGGGAGAGAA GAAGGATGTG TATAAGGCGG GTGAGCAAGT GACTTACACT TGTGCAACAT ATTACAAAAT GGATGGAGCC AGTAATGTAA CATGCATTAA TAGCAGATGG ACAGGAAGGC CAACATGCAG AGACACCTCC TGTGTGAATC CGCCCACAGT ACAAAATGCT TATATAGTGT CGAGACAGAT GAGTAAATAT CCATCTGGTG AGAGAGTACG TTATCAATGT AGGAGCCCTT ATGAAATGTT TGGGGATGAA GAAGTGATGT GTTTAAATGG AAACTGGACG GAACCACCTC AATGCAAAGA TTCTACAGGA AAATGTGGGC CCCCTCCACC TATTGACAAT GGGGACATTA CTTCATTCCC GTTGTCAGTA TATGCTCCAG CTTCATCAGT TGAGTACCAA TGCCAGAACT TGTATCAACT TGAGGGTAAC AAGCGAATAA CATGTAGAAA TGGACAATGG TCAGAACCAC CAAAATGCTT ACATCCGTGT GTAATATCCC GAGAAATTAT GGAAAATTAT AACATAGCAT TAAGGTGGAC AGCCAAACAG AAGCTTTATT CGAGAACAGG TGAATCAGTT GAATTTGTGT GTAAACGGGG ATATCGTCTT TCATCACGTT CTCACACATT GCGAACAACA TGTTGGGATG GGAAACTGGA GTATCCAACT TGTGCAAAAA GAGAATTCGA CTACAAGGAC GACGATGACA AGTGACTCGA GTCTAGAGGG CCCCTTAAGG GTTCGATCCC TCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGAAACGG GGGAGGCTAA CTGAAACACG GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGGTGT TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG TCGATACCCC ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC CCCACCCCCC AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC CTGCCATAGC AGATCTGCGC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGCATCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGGGGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA TGGTCGACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TCTGCTCCCT GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC AACAAGGCAA GGCTTGACCG ACAATTGCAT GAAGAATCTG CTTAGG