pCMV-2×Tet operator-FLAG-Pkm(mouse)
$70.00
In stock
SKU
PL40949
NM_011099.4;Pk-2; Pk-3; Pk3; Pkm2
100μg liquid plasmid(Instantly):$91
| name | pCMV-2×Tet operator-FLAG-Pkm(mouse) | another name | apCMV-2×Tet operator-FLAG-Pkm(mouse) |
| Insert Size (bp) | 1596bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV-To | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CCCTATCAGT GATAGAGATC TCCCTATCAG TGATAGAGAT CGTCGACGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGGA CTCTAGCGTT TAAACTTAAG CTTGCTAGCG CTACCGGACT CAGATCTCGA GCTCAAGCTT CGAATTCTGC AGTCGACGGT ACCGGCCACC ATGGACTACA AGGACGACGA TGACAAGATG CCGAAGCCAC ACAGTGAAGC AGGGACTGCC TTCATTCAGA CCCAGCAGCT CCATGCAGCC ATGGCTGACA CCTTCCTGGA ACACATGTGC CGCCTGGACA TTGACTCTGC CCCCATCACG GCCCGCAACA CTGGCATCAT TTGTACCATT GGGCCTGCTT CCCGATCTGT GGAGATGCTG AAGGAGATGA TTAAGTCTGG AATGAATGTG GCTCGGCTGA ATTTCTCTCA TGGAACCCAT GAGTACCATG CAGAGACCAT CAAGAATGTC CGTGAAGCCA CAGAAAGCTT TGCATCTGAT CCCATTCTCT ACCGTCCTGT TGCGGTGGCT CTGGATACAA AGGGACCTGA GATCCGGACT GGACTCATCA AGGGCAGCGG CACCGCTGAG GTGGAGCTGA AGAAGGGAGC CACTCTGAAG ATCACCCTGG ACAACGCTTA CATGGAGAAG TGTGACGAGA ACATCCTGTG GCTGGACTAC AAGAACATCT GCAAGGTGGT GGAGGTGGGC AGCAAGATCT ACGTGGACGA TGGGCTCATC TCACTGCAGG TGAAGGAGAA AGGCGCTGAC TTCCTGGTGA CGGAGGTGGA GAATGGTGGC TCCTTGGGCA GCAAGAAGGG CGTGAACCTG CCGGGCGCTG CTGTGGATCT CCCCGCTGTG TCGGAAAAGG ACATCCAGGA CCTGAAGTTT GGGGTGGAGC AGGATGTGGA CATGGTGTTT GCATCTTTCA TCCGCAAGGC AGCCGACGTG CATGAAGTCA GGAAGGTGCT GGGAGAGAAG GGCAAGAACA TCAAGATCAT CAGCAAAATC GAGAACCATG AAGGCGTCCG CAGGTTTGAT GAGATCTTGG AGGCCAGTGA TGGGATCATG GTGGCTCGTG GTGACCTGGG CATTGAGATT CCTGCAGAGA AGGTCTTCCT GGCTCAGAAG ATGATGATCG GGCGATGCAA CCGAGCTGGG AAGCCTGTCA TCTGTGCCAC ACAGATGCTG GAGAGCATGA TCAAGAAGCC ACGCCCCACC CGTGCTGAAG GCAGTGATGT GGCCAATGCA GTCCTGGATG GAGCAGACTG CATCATGCTG TCTGGAGAAA CAGCCAAGGG GGACTACCCT CTGGAGGCTG TTCGCATGCA GCACCTGATT GCCCGAGAGG CAGAGGCTGC CATCTACCAC TTGCAGCTAT TCGAGGAACT CCGCCGCCTG GCGCCCATTA CCAGCGACCC CACAGAAGCT GCCGCCGTGG GTGCCGTGGA GGCCTCCTTC AAGTGCTGCA GTGGGGCCAT TATCGTGCTC ACCAAGTCTG GCAGGAGTGC TCACCAAGTG GCCAGGTACC GCCCTCGGGC TCCTATCATT GCCGTGACTC GAAATCCCCA GACTGCTCGC CAGGCCCATC TGTACCGTGG CATCTTCCCT GTGCTGTGTA AGGATGCCGT GCTGAATGCC TGGGCTGAGG ATGTCGACCT TCGTGTAAAC TTGGCCATGG ATGTTGGCAA GGCCCGAGGC TTCTTCAAGA AGGGAGATGT GGTCATTGTG CTGACCGGGT GGCGCCCTGG CTCTGGATTC ACCAACACCA TGCGTGTAGT GCCTGTACCT TGAGGATCCC GCGACTCTAG AGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTGTGCCAG GGCGTGCCCT TGGGCTCCCC GGGCGCGTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTC
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
