NM_003334.4;A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1;POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS
100μg liquid plasmid(Instantly):$120
| name | pCMV-UBA1(human)-3×FLAG-Neo | another name | apCMV-UBA1(human)-3×FLAG-Neo |
| Insert Size (bp) | 3177bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCATGTC CAGCTCGCCG CTGTCCAAGA AACGTCGCGT GTCCGGGCCT GATCCAAAGC CGGGTTCTAA CTGCTCCCCT GCCCAGTCCG TGTTGTCCGA AGTGCCCTCG GTGCCAACCA ACGGAATGGC CAAGAACGGC AGTGAAGCAG ACATAGACGA GGGCCTTTAC TCCCGGCAGC TGTATGTGTT GGGCCATGAG GCAATGAAGC GGCTCCAGAC ATCCAGTGTC CTGGTATCAG GCCTGCGGGG CCTGGGCGTG GAGATCGCTA AGAACATCAT CCTTGGTGGG GTCAAGGCTG TTACCCTACA TGACCAGGGC ACTGCCCAGT GGGCTGATCT TTCCTCCCAG TTCTACCTGC GGGAGGAGGA CATCGGTAAA AACCGGGCCG AGGTATCACA GCCCCGCCTC GCTGAGCTCA ACAGCTATGT GCCTGTCACT GCCTACACTG GACCCCTCGT TGAGGACTTC CTTAGTGGTT TCCAGGTGGT GGTGCTCACC AACACCCCCC TGGAGGACCA GCTGCGAGTG GGTGAGTTCT GTCACAACCG TGGCATCAAG CTGGTGGTGG CAGACACGCG GGGCCTGTTT GGGCAGCTCT TCTGTGACTT TGGAGAGGAA ATGATCCTCA CAGATTCCAA TGGGGAGCAG CCACTCAGTG CTATGGTTTC TATGGTTACC AAGGACAACC CCGGTGTGGT TACCTGCCTG GATGAGGCCC GACACGGGTT TGAGAGCGGG GACTTTGTCT CCTTTTCAGA AGTACAGGGC ATGGTTGAAC TCAACGGAAA TCAGCCCATG GAGATCAAAG TCCTGGGTCC TTATACCTTT AGCATCTGTG ACACCTCCAA CTTCTCCGAC TACATCCGTG GAGGCATCGT CAGTCAGGTC AAAGTACCTA AGAAGATTAG CTTTAAATCC TTGGTGGCCT CACTGGCAGA ACCTGACTTT GTGGTGACGG ACTTCGCCAA GTTTTCTCGC CCTGCCCAGC TGCACATTGG CTTCCAGGCC CTGCACCAGT TCTGTGCTCA GCATGGCCGG CCACCTCGGC CCCGCAATGA GGAGGATGCA GCAGAACTGG TAGCCTTAGC ACAGGCTGTG AATGCTCGAG CCCTGCCAGC AGTGCAGCAA AATAACCTGG ACGAGGACCT CATCCGGAAG CTGGCATATG TGGCTGCTGG GGATCTGGCA CCCATAAACG CCTTCATTGG GGGCCTGGCT GCCCAGGAAG TCATGAAGGC CTGCTCCGGG AAGTTCATGC CCATCATGCA GTGGCTATAC TTTGATGCCC TTGAGTGTCT CCCTGAGGAC AAAGAGGTCC TCACAGAGGA CAAGTGCCTC CAGCGCCAGA ACCGTTATGA CGGGCAAGTG GCTGTGTTTG GCTCAGACCT GCAAGAGAAG CTGGGCAAGC AGAAGTATTT CCTGGTGGGT GCGGGGGCCA TTGGCTGTGA GCTGCTCAAG AACTTTGCCA TGATTGGGCT GGGCTGCGGG GAGGGTGGAG AAATCATCGT TACAGACATG GACACCATTG AGAAGTCAAA TCTGAATCGA CAGTTTCTTT TCCGGCCCTG GGATGTCACG AAGTTAAAGT CTGACACGGC TGCTGCAGCT GTGCGCCAAA TGAATCCACA TATCCGGGTG ACAAGCCACC AGAACCGTGT GGGTCCTGAC ACGGAGCGCA TCTATGATGA CGATTTTTTC CAAAACCTAG ATGGCGTGGC CAATGCCCTG GACAACGTGG ATGCCCGCAT GTACATGGAC CGCCGCTGTG TCTACTACCG GAAGCCACTG CTGGAGTCAG GCACACTGGG CACCAAAGGC AATGTGCAGG TGGTGATCCC CTTCCTGACA GAGTCGTACA GTTCCAGCCA GGACCCACCT GAGAAGTCCA TCCCCATCTG TACCCTGAAG AACTTCCCTA ATGCCATCGA GCACACCCTG CAGTGGGCTC GGGATGAGTT TGAAGGCCTC TTCAAGCAGC CAGCAGAAAA TGTCAACCAG TACCTCACAG ACCCCAAGTT TGTGGAGCGA ACACTGCGGC TGGCAGGCAC TCAGCCCTTG GAGGTGCTGG AGGCTGTGCA GCGCAGCCTG GTGCTGCAGC GACCACAGAC CTGGGCTGAC TGCGTGACCT GGGCCTGCCA CCACTGGCAC ACCCAGTACT CGAACAACAT CCGGCAGCTG CTGCACAACT TCCCTCCTGA CCAGCTCACA AGCTCAGGAG CGCCGTTCTG GTCTGGGCCC AAACGCTGTC CACACCCGCT CACCTTTGAT GTCAACAATC CCCTGCATCT GGACTATGTG ATGGCTGCTG CCAACCTGTT TGCCCAGACC TACGGGCTGA CAGGCTCTCA GGACCGAGCT GCTGTGGCCA CATTCCTGCA GTCTGTGCAG GTCCCCGAAT TCACCCCCAA GTCTGGCGTC AAGATCCATG TTTCTGACCA GGAGCTGCAG AGCGCCAATG CCTCTGTTGA TGACAGTCGT CTAGAGGAGC TCAAAGCCAC TCTGCCCAGC CCAGACAAGC TCCCTGGATT CAAGATGTAC CCCATTGACT TTGAGAAGGA TGATGACAGC AACTTTCATA TGGATTTCAT CGTGGCTGCA TCCAACCTCC GGGCAGAAAA CTATGACATT CCTTCTGCAG ACCGGCACAA GAGCAAGCTG ATTGCAGGGA AGATCATCCC AGCCATTGCC ACGACCACAG CAGCCGTGGT TGGCCTTGTG TGTCTGGAGC TGTACAAGGT TGTGCAGGGG CACCGACAGC TTGACTCCTA CAAGAATGGT TTCCTCAACT TGGCCCTGCC TTTCTTTGGT TTCTCTGAAC CCCTTGCCGC ACCACGTCAC CAGTACTATA ACCAAGAGTG GACATTGTGG GATCGCTTTG AGGTACAAGG GCTGCAGCCT AATGGTGAGG AGATGACCCT CAAACAGTTC CTCGACTATT TTAAGACAGA GCACAAATTA GAGATCACCA TGCTGTCCCA GGGCGTGTCC ATGCTCTATT CCTTCTTCAT GCCAGCTGCC AAGCTCAAGG AACGGTTGGA TCAGCCGATG ACAGAGATTG TGAGCCGTGT GTCGAAGCGA AAGCTGGGCC GCCACGTGCG GGCGCTGGTG CTTGAGCTGT GCTGTAACGA CGAGAGCGGC GAGGATGTCG AGGTTCCCTA TGTCCGATAC ACCATCCGCG GATCCGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA GTAGTGAGGG CCCGATATCT CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCCTGGAAA CGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCAGATCTG CGCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGCATCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGG GGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCGACGGA TCGGGAGATC TCCCGATCCC CTATGGTCGA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATCTGCTC CCTGCTTGTG TGTTGGAGGT CGCTGAGTAG TGCGCGAGCA AAATTTAAGC TACAACAAGG CAAGGCTTGA CCGACAATTG CATGAAGAAT CTGCTTAGG
