NM_020954.3;ALO17; C17orf27; KIAA1618; MYMY2; MYSTR;NET57
100μg liquid plasmid(Instantly):$120
| name | pCMV-TagBFP-RNF213(human)-Neo | another name | apCMV-TagBFP-RNF213(human)-Neo |
| Insert Size (bp) | 3192bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Blue |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCCA CCATGAGCGA GCTGATTAAG GAGAACATGC ACATGAAGCT GTACATGGAG GGCACCGTGG ACAACCATCA CTTCAAGTGC ACATCCGAGG GCGAAGGCAA GCCCTACGAG GGCACCCAGA CCATGAGAAT CAAGGTGGTC GAGGGCGGCC CTCTCCCCTT CGCCTTCGAC ATCCTGGCTA CTAGCTTCCT CTACGGCAGC AAGACCTTCA TCAACCACAC CCAGGGCATC CCCGACTTCT TCAAGCAGTC CTTCCCTGAG GGCTTCACAT GGGAGAGAGT CACCACATAC GAAGACGGGG GCGTGCTGAC CGCTACCCAG GACACCAGCC TCCAGGACGG CTGCCTCATC TACAACGTCA AGATCAGAGG GGTGAACTTC ACATCCAACG GCCCTGTGAT GCAGAAGAAA ACACTCGGCT GGGAGGCCTT CACCGAGACG CTGTACCCCG CTGACGGCGG CCTGGAAGGC AGAAACGACA TGGCCCTGAA GCTCGTGGGC GGGAGCCATC TGATCGCAAA CATCAAGACC ACATATAGAT CCAAGAAACC CGCTAAGAAC CTCAAGATGC CTGGCGTCTA CTATGTGGAC TACAGACTGG AAAGAATCAA GGAGGCCAAC AACGAGACCT ACGTCGAGCA GCACGAGGTG GCAGTGGCCA GATACTGCGA CCTCCCTAGC AAACTGGGGC ACAAGCTTAA TGGCGGCGGA GGGAGTGGAG GAGGGGGAAG CGGAGGCGGA GGGAGTCTCG AGATGGAGTG TCCTTCGTGC CAGCATGTCT CCAAGGAGGA AACCCCCAAG TTCTGCAGCC AGTGCGGAGA GAGGCTGCCT CCTGCAGCCC CCATAGCAGA TTCTGAGAAC AATAACTCCA CAATGGCGTC GGCCTCGGAG GGTGAAATGG AGTGTGGGCA GGAGCTGAAG GAGGAAGGGG GCCCGTGCTT GTTCCCGGGC TCAGACAGTT GGCAAGAAAA CCCCGAGGAG CCCTGTTCCA AAGCCTCCTG GACCGTCCAA GAAAGCAAAA AGAAGAAAAG GAAGAAGAAA AAGAAGGGGA ACAAGTCCGC TTCCTCAGAG CTGGCTTCCT TGCCCCTTTC TCCTGCCAGC CCCTGTCACC TGACTTTGCT TTCAAACCCG TGGCCTCAGG ACACAGCCCT GCCCCACAGC CAAGCCCAGC AGAGTGGCCC CACTGGCCAG CCGAGCCAGC CCCCAGGCAC AGCCACCACG CCACTGGAGG GTGACGGCCT CTCCGCGCCC ACCGAGGTTG GCGACAGCCC CCTGCAGGCC CAGGCTTTGG GAGAGGCAGG AGTGGCCACA GGAAGTGAGG CTCAGAGCAG CCCGCAATTC CAGGACCACA CGGAAGGGGA GGACCAGGAC GCTTCCATCC CCTCTGGGGG CAGAGGCCTG TCCCAGGAGG GGACCGGTCC CCCCACCTCT GCTGGTGAAG GCCATTCTAG GACTGAAGAT GCTGCCCAGG AGCTCCTGTT GCCTGAGTCA AAAGGAGGCA GCTCTGAGCC CGGGACAGAA CTGCAGACCA CCGAGCAACA GGCAGGGGCC TCAGCCTCTA TGGCAGTTGA TGCTGTAGCT GAGCCAGCCA ATGCAGTTAA AGGGGCCGGG AAGGAAATGA AAGAGAAGAC CCAGAGAATG AAACAGCCAC CAGCAACCAC TCCTCCTTTC AAAACACACT GCCAGGAAGC TGAGACCAAG ACCAAGGACG AGATGGCTGC TGCTGAAGAA AAAGTCGGTA AGAATGAACA AGGGGAGCCT GAAGACCTCA AGAAGCCAGA GGGGAAGAAC AGAAGTGCAG CTGCTGTGAA AAACGAGAAG GAGCAAAAAA ACCAGGAAGC AGATGTCCAG GAAGTGAAGG CAAGCACGCT GAGCCCGGGT GGAGGAGTCA CCGTGTTCTT CCACGCCATC ATCTCTCTTC ATTTCCCATT CAATCCTGAC CTCCATAAAG TCTTCATCAG AGGAGGAGAA GAATTTGGGG AGTCAAAATG GGACAGCAAT ATCTGTGAGC TGCACTACAC CAGAGACTTG GGTCATGACC GCGTTCTTGT TGAAGGCATT GTCTGCATTT CCAAGAAGCA CCTAGATAAA TACATTCCTT ACAAGTACGT CATTTATAAT GGGGAATCTT TTGAGTATGA GTTCATTTAC AAGCACCAGC AGAAGAAGGG CGAGTACGTC AACCGCTGTC TGTTCATAAA ATCTTCACTT CTGGGCTCAG GAGACTGGCA TCAGTACTAT GACATAGTTT ATATGAAGCC TCATGGGAGA CTCCAGAAAG TCATGAACCA CATCACAGAC GGGCCGAGGA AGGACCTGGT GAAGGGGAAG CAGATTGCCG CTGCGCTCAT GCTGGACAGC ACCTTCAGCA TCCTGCAGAC CTGGGACACC ATCAACCTGA ACAGCTTCTT CACCCAGTTC GAGCAGTTTT GCTTTGTCCT GCAACAGCCT ATGATTTATG AAGGACAGGC ACAGCTGTGG ACCGATTTGC AGTACAGGGA GAAAGAGGTG AAGAGATACC TGTGGCAACA TCTGAAAAAA CACGTGGTAC CATTGCCGGA CGGAAAAAGC ACGGACTTTT TGCCTGTGGA CTGCCCAGTG AGGAGTAAAC TGAAAACAGG CCTGATTGTC CTTTTTGTAG TGGAAAAAAT TGAGCTTTTA TTAGAAGGCA GCCTGGACTG GTTGTGTCAC CTCCTAACCT CAGATGCCAG CTCACCAGAT GAGTTTCACC GTGACCTAAG CCACATCCTT GGGATACCTC AGAGCTGGCG GCTGTACCTG GTGAACCTGT GCCAAAGATG CATGGACACA AGGACGTACA CCTGGCTGGG CGCCCTGCCT GTCCTGCACT GCTGTATGGA GCTGGCCCCG CGGCACAAGG ATGCCTGGAG ACAGCCTGAG GACACCTGGG CCGCTCTGGA GGGACTCTCC TTCTCACCGT TCCGGGAACA AATGCTAGAT ACGAGTTCCC TACTTCAGTT TATGAGAGAG AAGCAGCATT TGCTGAGCAT AGACGAGCCT CTCTTCCGGT CCTGGTTTAG TCTGCTACCT CTGAGTCACC TGGTTATGTA TATGGAAAAC TTCATTGAGC ACCTGGGTCG TTTTCCTGCT CATATCCTGG ACTGTCTTTC AGGGATTTAC TACCGGCTTC CGGGACTTGA GCAAGTCTTG AATACGCAGG ATGTTCAGGA TGTTCAGAAC GTTCAGAACA TTTTAGAAAT GCTGTTGCGA CTCCTGGACA CTTACCGGGA CAAGATTCCC GAGGAGGCCT TGTCACCATC CTACCTGACT GTGTGTCTGA AACTGCATGA AGCCATCTGC AGCAGCACAA AGCTACTTAA GTTTTACGAG CTGCCAGCCT TATCTGCCGA GATTGTCTGC AGAATGATTA GACTTCTATC TCTGGTGGAT TCTGCAGGAC AGAGAGATGA AACTGGAAAT AATTCAGTCC AAACAGTCTT CCAAGGGACC CTTGCTGCTA CGAAAAGGTG GCTCCGAGAA GTTTTTACAA AGAACATGCT CACATCTTCA GGTGCCTCAT TCACATACGT CAAGGAAATT GAGGTCTGGA GGCGGCTGGT GGAAATCCAA TTCCCCGCGG AGCATGGCTG GAAGGAGTCG TTGCTGGGAG ACATGGAATG GAGGCTCACA AAGGAGGAAC CCCTCTCCCA GATCACTGCC TACTGCAATA GTTGCTGGGA CACCAAAGGC TTAGAGGACA GTGTGGCCAA GACCTTCGAG AAATGCATCA TTGAAGCCGT GAGCTCAGCC TGCCAGGTGA ACAATCTCTC CTCCTGGGAA ACGGATTCGG GCTCACAGCT GTGTTCTGCC ATGACCCAGC TAAGGGCTAT GAAGCACCCG CTGGGTCTCA GCTCCTCCGC TAACTCAGAG ATTGGGAAGT GGGCACCCTC CTCCCTCGCC AAGGGCAATG GCGCTGAAAT CTAGGAATTC TGCAGTCGAC GGTACCGCGG GCCCGGGATC CACCGGTGCG GCCGCGACTC TAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA AGGCGTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTCCTGAGG CGGAAAGAAC CAGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCTAGGGGG AGGCTAACTG AAACACGGAA GGAGACAATA CCGGAAGGAA CCCGCGCTAT GACGGCAATA AAAAGACAGA ATAAAACGCA CGGTGTTGGG TCGTTTGTTC ATAAACGCGG GGTTCGGTCC CAGGGCTGGC ACTCTGTCGA TACCCCACCG AGACCCCATT GGGGCCAATA CGCCCGCGTT TCTTCCTTTT CCCCACCCCA CCCCCCAAGT TCGGGTGAAG GCCCAGGGCT CGCAGCCAAC GTCGGGGCGG CAGGCCCTGC CATAGCCTCA GGTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCATGCA T
