NM_001130173.2;c-myb; c-myb_CDS; Cmyb; efg
100μg liquid plasmid(Instantly):$105
| name | pLV2-TRE3GS-Fluc-MYB(human)-6×His-TetOne-Hyg | another name | apLV2-TRE3GS-Fluc-MYB(human)-6×His-TetOne-Hyg |
| Insert Size (bp) | 2286bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Hyg | Fluorescent labeling | Fluc |
| Promoter | TRE3GS | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTACTAAGT TTGTAGTACA TATTTAACAA ATACAATTTC TTTAAAAACT ACTAAGTTTG TAGTACATAT TTAACAAATA CAATTTCTTT AAAATGAAAA TAATTCAGAG GAATCACAGG TTTAGAGTAA ATGAAACCAC TAGGTAATTG GCAGTGGTAA TAGGGTATGG GGTGGGAAGT TTGGGATGAT TTTGGTTAGC TTGAGTTATC CAGTTGATCC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT GATCTCCCGA TCCGTCGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCT AGCTAGAGGT CGACGGTAAA ACGCTAGCAA TGTCAAGGCC TCTCACTCTC TGATATTCAT TTCTTTGCAA GTTATAAATA CTGAATAATA AGATGACATG AACTACTACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGGCA AAAAGCAGCT GCTTATATGC AGGATCTGAG GGCTCGCCAC TCCCCAGTCC CGCCCAGGCC ACGCCTCCCT GGAAAGTCCC CAGCGGAAAG TCCCTTGTAG CAAGCTCGAT ATCAGCAGTT CTTGAAGTAC TCCGGATGCA GCTCTCGGGC CACGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AACACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCTGGTG TTCTCTCCTT TATTGGCCTC TTCTACCTTA TCTGGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGACCCCT GGCCCTGGTG TGTAGTTCTG CTAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCT CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACTGCAGAAT TAATTCCAGG CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG CGAAGGCGAA GACGCGGAAG AGGCCGCAGA GCCGGCAGCA GGCCGCGGGA AGGAAGGTCC GCTGGATTGA GGGCCGAAGG GACGTAGCAG AAGGACGTCC CGCGCAGAAT CCAGGTGGCA ACACAGGCGA GCAGCCATGG AAAGGACGTC AGCTTCCCCG ACAACACCAC GGAATTGTCA GTGCCCAACA GCCGAGCCCC TGTCCAGCAG CGGGCAAGGC AGGCGGCGAT GAGTTCCGCC GTGGCAATAG GGAGGGGGAA AGCGAAAGTC CCGGAAAGGA GCTGACAGGT GGTGGCAATG CCCCAACCAG TGGGGGTTGC GTCAGCAAAC ACAGTGCACA CCACGCCACG TTGCCTGACA ACGGGCCACA ACTCCTCATA AAGAGACAGC AACCAGGATT TATACAAGGA GGAGAAAATG AAAGCCATAC GGGAAGCAAT AGCATGATAC AAAGGCATTA AAGCAGCGTA TCCACATAGC GTAAAAGGAG CAACATAGTT AAGAATACCA GTCAATCTTT CACAAATTTT GTAATCCAGA GGTTGATTGT TCCAGACGCG TCTATTCCTT TGCCCTCGGA CGAGTGCTGG GGCGTCGGTT TCCACTATCG GCGAGTACTT CTACACAGCC ATCGGTCCAG ACGGCCGCGC TTCTGCGGGC GATTTGTGTA CGCCCGACAG TCCCGGCTCC GGATCGGACG ATTGCGTCGC ATCGACCCTG CGCCCAAGCT GCATCATCGA AATTGCCGTC AACCAAGCTC TGATAGAGTT GGTCAAGACC AATGCGGAGC ATATACGCCC GGAGCCGCGG CGATCCTGCA AGCTCCGGAT GCCTCCGCTC GAAGTAGCGC GTCTGCTGCT CCATACAAGC CAACCACGGC CTCCAGAAGA AGATGTTGGC GACCTCGTAT TGGGAATCCC CGAACATCGC CTCGCTCCAG TCAATGACCG CTGTTATGCG GCCATTGTCC GTCAGGACAT TGTTGGAGCC GAAATCCGCG TGCACGAGGT GCCGGACTTC GGGGCAGTCC TCGGCCCAAA GCATCAGCTC ATCGAGAGCC TGCGCGACGG ACGCACTGAC GGTGTCGTCC ATCACAGTTT GCCAGTGATA CACATGGGGA TCAGCAATCG CGCATATGAA ATCACGCCAT GTAGTGTATT GACCGATTCC TTGCGGTCCG AATGGGCCGA ACCCGCTCGT CTGGCTAAGA TCGGCCGCAG CGATCGCATC CATGGCCTCC GCGACCGGCT GCAGAACAGC GGGCAGTTCG GTTTCAGGCA GGTCTTGCAA CGTGACACCC TGTGCACGGC GGGAGATGCA ATAGGTCAGG CTCTCGCTGA ATTCCCCAAT GTCAAGCACT TCCGGAATCG GGAGCGCGGC CGATGCAAAG TGCCGATAAA CATAACGATC TTTGTAGAAA CCATCGGCGC AGCTATTTAC CCGCAGGACA TATCCACGCC CTCCTACATC GAAGCTGAAA GCACGAGATT CTTCGCCCTC CGAGAGCTGC ATCAGGTCGG AGACGCTGTC GAACTTTTCG ATCAGAAACT TCTCGACAGA CGTCGCGGTG AGTTCAGGCT TTTTCATCAC GTGCTGATCA GATCCGAAAA TGGATATACA AGCTCCCGGG AGCTTTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCG CGCGTTTACC CGGGGAGCAT GTCAAGGTCA AAATCGTCAA GAGCGTCAGC AGGCAGCATA TCAAGGTCAA AGTCGTCAAG GGCATCGGCT GGGAGCATGT CTAAGTCAAA ATCGTCAAGG GCGTCGGTCG GCCCGCCGCT TTCGCACTTT AGCTGTTTCT CCAGGCCACA TATGATTAGT TCCAGGCCGA AAAGGAAGGC AGGTTCGGCT CCCTGCCGGT CGAACAGCTC AATTGCTTGT TTCAGAAGTG GGGGCATAGA ATCGGTGGTA GGTGTCTCTC TTTCCTCTTT TGCTACTTGA TGCTCCTGTT CCTCCAATAC GCAGCCCAGT GTAAAGTGGC CCACGGCGGA CAGAGCGTAC AGTGCGTTCT CCAGGGAGAA GCCTTGCTGA CACAGGAACG CGAGCTGATT TTCCAGGGTT TCGTACTGTT TCTCTGTTGG GCGGGTGCCG AGATGCACTT TAGCCCCGTC GCGATGTGAG AGGAGAGCAC AGCGGTATGA CTTGGCGTTG TTCCGCAGAA AGTCTTGCCA TGACTCGCCT TCCAGGGGGC AGGAGTGGGT ATGATGCCTG TCCAGCATCT CGATTGGCAG GGCATCGAGC AGGGCCCGCT TGTTCTTCAC GTGCCAGTAC AGGGTAGGCT GCTCAACTCC CAGCTTTTGA GCGAGTTTCC TTGTCGTCAG GCCTTCGATA CCGACTCCAT TGAGTAATTC CAGAGCAGAG TTTATGACTT TGCTCTTGTC CAGTCTAGAC ATGGTAATTC GATGATCCCC CTGGGGAGAG AGGTCGGTGA TTCGGTCAAC GAGGGAGCCG ACTGCCGACG TGCGCTCCGG AGGCTTGCAG AATGCGGAAC ACCGCGCGGG CAGGAACAGG GCCCACACTA CCGCCCCACA CCCCGCCTCC CGCACCGCCC CTTCCCGGCC GCTGCTCTCG GCGCGCCCTG CTGAGCAGCC GCTATTGGCC ACAGCCCATC GCGGTCGGCG CGCTGCCATT GCTCCCTGGC GCTGTCCGTC TGCGAGGGTA CTAGTGAGAC GTGCGGCTTC CGTTTGTCAC GTCCGGCACG CCGCGAACCG CAAGGAACCT TCCCGACTTA GGGGCGGAGC AGGAAGCGTC GCCGGGGGGC CCACAAGGGT AGCGGCGAAG ATCCGGGTGA CGCTGCGAAC GGACGTGAAG AATGTGCGAG ACCCAGGGTC GGCGCCGCTG CGTTTCCCGG AACCACGCCC AGAGCAGCCG CGTCCCTGCG CAAACCCAGG GCTGCCTTGG AAAAGGCGCA ACCCCAACCC CGTGGAATTA TCACCTCGAG TTTACTCCCT ATCAGTGATA GAGAACGTAT GAAGAGTTTA CTCCCTATCA GTGATAGAGA ACGTATGCAG ACTTTACTCC CTATCAGTGA TAGAGAACGT ATAAGGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGA CCAGTTTACT CCCTATCAGT GATAGAGAAC GTATCTACAG TTTACTCCCT ATCAGTGATA GAGAACGTAT ATCCAGTTTA CTCCCTATCA GTGATAGAGA ACGTATAAGC TTTGCTTATG TAAACCAGGG CGCCTATAAA AGAGTGCTGA TTTTTTGAGT AAACTTCAAT TCCACAACAC TTTTGTCTTA TACCAACTTT CCGTACCACT TCCTACCCTC GTAAAGAATT CCCGTATACA CCGGTGCCAC CATGGAAGAT GCCAAAAACA TTAAGAAGGG CCCAGCGCCA TTCTACCCAC TCGAAGACGG GACCGCCGGC GAGCAGCTGC ACAAAGCCAT GAAGCGCTAC GCCCTGGTGC CCGGCACCAT CGCCTTTACC GACGCACATA TCGAGGTGGA CATTACCTAC GCCGAGTACT TCGAGATGAG CGTTCGGCTG GCAGAAGCTA TGAAGCGCTA TGGGCTGAAT ACAAACCATC GGATCGTGGT GTGCAGCGAG AATAGCTTGC AGTTCTTCAT GCCCGTGTTG GGTGCCCTGT TCATCGGTGT GGCTGTGGCC CCAGCTAACG ACATCTACAA CGAGCGCGAG CTGCTGAACA GCATGGGCAT CAGCCAGCCC ACCGTCGTAT TCGTGAGCAA GAAAGGGCTG CAAAAGATCC TCAACGTGCA AAAGAAGCTA CCGATCATAC AAAAGATCAT CATCATGGAT AGCAAGACCG ACTACCAGGG CTTCCAAAGC ATGTACACCT TCGTGACTTC CCATTTGCCA CCCGGCTTCA ACGAGTACGA CTTCGTGCCC GAGAGCTTCG ACCGGGACAA AACCATCGCC CTGATCATGA ACAGTAGTGG CAGTACCGGA TTGCCCAAGG GCGTAGCCCT ACCGCACCGC ACCGCTTGTG TCCGATTCAG TCATGCCCGC GACCCCATCT TCGGCAACCA GATCATCCCC GACACCGCTA TCCTCAGCGT GGTGCCATTT CACCACGGCT TCGGCATGTT CACCACGCTG GGCTACTTGA TCTGCGGCTT TCGGGTCGTG CTCATGTACC GCTTCGAGGA GGAGCTATTC TTGCGCAGCT TGCAAGACTA TAAGATTCAA TCTGCCCTGC TGGTGCCCAC ACTATTTAGC TTCTTCGCTA AGAGCACTCT CATCGACAAG TACGACCTAA GCAACTTGCA CGAGATCGCC AGCGGCGGGG CGCCGCTCAG CAAGGAGGTA GGTGAGGCCG TGGCCAAACG CTTCCACCTA CCAGGCATCC GCCAGGGCTA CGGCCTGACA GAAACAACCA GCGCCATTCT GATCACCCCC GAAGGGGACG ACAAGCCTGG CGCAGTAGGC AAGGTGGTGC CCTTCTTCGA GGCTAAGGTG GTGGACTTGG ACACCGGTAA GACACTGGGT GTGAACCAGC GCGGCGAGCT GTGCGTCCGT GGCCCCATGA TCATGAGCGG CTACGTTAAC AACCCCGAGG CTACAAACGC TCTCATCGAC AAGGACGGCT GGCTGCACAG CGGCGACATC GCCTACTGGG ACGAGGACGA GCACTTCTTC ATCGTGGACC GGCTGAAGAG CCTGATCAAA TACAAGGGCT ACCAGGTAGC CCCAGCCGAA CTGGAGAGCA TCCTGCTGCA ACACCCCAAC ATCTTCGACG CCGGGGTCGC CGGCCTGCCC GACGACGATG CCGGCGAGCT GCCCGCCGCA GTCGTCGTGC TGGAACACGG TAAAACCATG ACCGAGAAGG AGATCGTGGA CTATGTGGCC AGCCAGGTTA CAACCGCCAA GAAGCTGCGC GGTGGTGTTG TGTTCGTGGA CGAGGTGCCT AAAGGACTGA CCGGCAAGTT GGACGCCCGC AAGATCCGCG AGATTCTCAT TAAGGCCAAG AAGGGCGGCA AGATCGCCGT GGGAGGTGGA GGTTCAATGG CCCGAAGACC CCGGCACAGC ATATATAGCA GTGACGAGGA TGATGAGGAC TTTGAGATGT GTGACCATGA CTATGATGGG CTGCTTCCCA AGTCTGGAAA GCGTCACTTG GGGAAAACAA GGTGGACCCG GGAAGAGGAT GAAAAACTGA AGAAGCTGGT GGAACAGAAT GGAACAGATG ACTGGAAAGT TATTGCCAAT TATCTCCCGA ATCGAACAGA TGTGCAGTGC CAGCACCGAT GGCAGAAAGT ACTAAACCCT GAGCTCATCA AGGGTCCTTG GACCAAAGAA GAAGATCAGA GAGTGATAGA GCTTGTACAG AAATACGGTC CGAAACGTTG GTCTGTTATT GCCAAGCACT TAAAGGGGAG AATTGGAAAA CAATGTAGGG AGAGGTGGCA TAACCACTTG AATCCAGAAG TTAAGAAAAC CTCCTGGACA GAAGAGGAAG ACAGAATTAT TTACCAGGCA CACAAGAGAC TGGGGAACAG ATGGGCAGAA ATCGCAAAGC TACTGCCTGG ACGAACTGAT AATGCTATCA AGAACCACTG GAATTCTACA ATGCGTCGGA AGGTCGAACA GGAAGGTTAT CTGCAGGAGT CTTCAAAAGC CAGCCAGCCA GCAGTGGCCA CAAGCTTCCA GAAGAACAGT CATTTGATGG GTTTTGCTCA GGCTCCGCCT ACAGCTCAAC TCCCTGCCAC TGGCCAGCCC ACTGTTAACA ACGACTATTC CTATTACCAC ATTTCTGAAG CACAAAATGT CTCCAGTCAT GTTCCATACC CTGTAGCGTT ACATGTAAAT ATAGTCAATG TCCCTCAGCC AGCTGCCGCA GCCATTCAGA GACACTATAA TGATGAAGAC CCTGAGAAGG AAAAGCGAAT AAAGGAATTA GAATTGCTCC TAATGTCAAC CGAGAATGAG CTAAAAGGAC AGCAGGTGCT ACCAACACAG AACCACACAT GCAGCTACCC CGGGTGGCAC AGCACCACCA TTGCCGACCA CACCAGACCT CATGGAGACA GTGCACCTGT TTCCTGTTTG GGAGAACACC ACTCCACTCC ATCTCTGCCA GCGGATCCTG GCTCCCTACC TGAAGAAAGC GCCTCGCCAG CAAGGTGCAT GATCGTCCAC CAGGGCACCA TTCTGGATAA TGTTAAGAAC CTCTTAGAAT TTGCAGAAAC ACTCCAATTT ATAGATTCTG ATTCTTCATC ATGGTGTGAT CTCAGCAGTT TTGAATTCTT TGAAGAAGCA GATTTTTCAC CTAGCCAACA TCACACAGGC AAAGCCCTAC AGCTTCAGCA AAGAGAGGGC AATGGGACTA AACCTGCAGG AGAACCTAGC CCAAGGGTGA ACAAACGTAT GTTGAGTGAG AGTTCACTTG ACCCACCCAA GGTCTTACCT CCTGCAAGGC ACAGCACAAT TCCACTGGTC ATCCTTCGAA AAAAACGGGG CCAGGCCAGC CCCTTAGCCA CTGGAGACTG TAGCTCCTTC ATATTTGCTG ACGTCAGCAG TTCAACTCCC AAGCGTTCCC CTGTCAAAAG CCTACCCTTC TCTCCCTCGC AGTTCTTAAA CACTTCCAGT AACCATGAAA ACTCAGACTT GGAAATGCCT TCTTTAACTT CCACCCCCCT CATTGGTCAC AAATTGACTG TTACAACACC ATTTCATAGA GACCAGACTG TGAAAACTCA AAAGGAAAAT ACTGTTTTTA GAACCCCAGC TATCAAAAGG TCAATCTTAG AAAGCTCTCC AAGAACTCCT ACACCATTCA AACATGCACT TGCAGCTCAA GAAATTAAAT ACGGTCCCCT GAAGATGCTA CCTCAGACAC CCTCTCATCT AGTAGAAGAT CTGCAGGATG TGATCAAACA GGAATCTGAT GAATCTGGAA TTGTTGCTGA GTTTCAAGAA AATGGACCAC CCTTACTGAA GAAAATCAAA CAAGAGGTGG AATCTCCAAC TGATAAATCA GGAAACTTCT TCTGCTCACA CCACTGGGAA GGGGACAGTC TGAATACCCA ACTGTTCACG CAGACCTCGC CTGTGGCAGA TGCACCGAAT ATTCTTACAA GCTCCGTTTT AATGGCACCA GCATCAGAAG ATGAAGACAA TGTTCTCAAA GCATTTACAG TACCTAAAAA CAGGTCCCTG GCGAGCCCCT TGCAGCCTTG TAGCAGTACC TGGGAACCTG CATCCTGTGG AAAGATGGAG GAGCAGATGA CATCTTCCAG TCAAGCTCGT AAATACGTGA ATGCATTCTC AGCCCGGACG CTGGTCATGC ACCATCACCA TCACCATTAG GATCCAGACC ACCTCCCCTG CGAGCTAAGC TGGACAGCCA ATGACGGGTA AGAGAGTGAC ATTTTTCACT AACCTAAGAC AGGAGGGCCG TCAGAGCTAC TGCCTAATCC AAAGACGGGT AAAAGTGATA AAAATGTATC ACTCCAACCT AAGACAGGCG CAGCTTCCGA GGGATTTGAG ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CAAAACTAGA ATGCAGTGAA AAAAATGCCT TATTTGTGAA ATTTGTGATG CTATTGCCTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAAGTCG ATAAACTGGA TCTCTGCTGT CCCTGTAATA AACCCGAAAA TTTTGAATTT TTGTAATTTG TTTTTGTAGT TCTTTAGTTT GTATGTCTGT TGCTATTATG TCTACTATTC TTTCCCCTGC ACTGTACCCC CCAATCCCCC CTTTTCTTTT AAAGGCGATA CCGTCGAGAT CCGTTCACTA ATCGAATGGA TCTGTCTCTG TCTCTCTCTC CACCTTCTTC TTCTATTCCT TCGGGCCTGT CGGGTCCCCT CGGGGTTGGG AGGTGGGTCT GAAACGATAA TGGTGAATAT CCCTGCCTAA CTCTATTCAC TATAGAAAGT ACAGCAAAAA CTATTCTTAA ACCTACCAAG CCTCCTACTA TCATTATGAA TAATTTTATA TACCACAGCC AATTTGTTAT GTTAAACCAA TTCCACAAAC TTGCCCATTT ATCTAATTCC AATAATTCTT GTTCATTCTT TTCTTGCTGG TTTTGCGATT CTTCAATTAA GGAGTGTATT AAGCTTGTGT AATTGTTAAT TTCTCTGTCC CACTCCATCC AGGTCGTGTG ATTCCAAATC TGTTCCAGAG ATTTATTACT CCAACTAGCA TTCCAAGGCA CAGCAGTGGT GCAAATGAGT TTTCCAGAGC AACCCCAAAT CCCCAGGAGC TGTTGATCCT TTAGGTATCT TTCCACAGCC AGGATTCTTG CCTGGAGCTG CTTGATGCCC CAGACTGTGA GTTGCAACAG ATGCTGTTGC GCCTCAATAG CCCTCAGCAA ATTGTTCTGC TGCTGCACTA TACCAGACAA TAATTGTCTG GCCTGTACCG TCAGCGTCAT TGACGCTGCG CCCATAGTGC TTCCTGCTGC TCCCAAGAAC CCAAGGAACA AAGCTCCTAT TCCCACTGCT CTTTTTTCTC TCTGCACCAC TCTTCTCTTT GCCTTGGTGG GTGCTACTCC TAATGGTTCA ATTTTTACTA CTTTATATTT ATATAATTCA CTTCTCCAAT TGTCCCTCAT ATCTCCTCCT CCAGGTCTGA AGATCAGCGG CCGGCCGCTT GCTGTGCGGT GGTCTTACTT TTGTTTTGCT CTTCCTCTAT CTTGTCTAAA GCTTCCTTGG TGTCTTTTAT CTCTATCCTT TGATGCACAC AATAGAGGGT TGCTACTGTA TTATATAATG ATCTAAGTTC TTCTGATCCT GTCTGAAGGG ATGGTTGTAG CTGTCCCAGT ATTTGTCTAC AGCCTTCTGA TGTTTCTAAC AGGCCAGGAT TAACTGCGAA TCGTTCTAGC TCCCTGCTTG CCCATACTAT ATGTTTTAAT TTATATTTTT TCTTTCCCCC TGGCCTTAAC CGAATTTTTT CCCATCGCGA TCTAATTCTC CCCCGCTTAA TACTGACGCT CTCGCACCCA TCTCTCTCCT TCTAGCCTCC GCTAGTCAAA ATTTTTGGCG TACTCACCAG TCGCCGCCCC TCGCCTCTTG CCGTGCGCGC TTCAGCAAGC CGAGTCCTGC GTCGAGAGAG CTCCTCTGGT TTCCCTTTCG CTTTCAAGTC CCTGTTCGGG CGCCACTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGGCAAAA AGCAGCTGCT TATATGCAGG ATCTGAGGGC TCGCCACTCC CCAGTCCCGC CCAGGCCACG CCTCCCTGGA AAGTCCCCAG CGGAAAGTCC CTTGTAGCAA GCTCGATATC AGCAGTTCTT GAAGTACTCC GGATGCAGCT CTCGGGCCAC GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAC ACTTCTCTCT CCGGGTCATC CATCCCATGC AGGCTCACAG GGTGTAACAA GCTGGTGTTC TCTCCTTTAT TGGCCTCTTC TACCTTATCT GGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT GACCCCTGGC CCTGGTGTGT AGTTCTGCTA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT CCACAGATCA AGGATATCTT GTCTTCTTTG GGAGTGAATT AGCCCTTCCA
