pCMV-BNC1(human)-EGFP
$84.00
In stock
SKU
PL40809
NM_001717.4;BNC; BSN1; HsT19447; POF16
100μg liquid plasmid(Instantly):$105
| name | pCMV-BNC1(human)-EGFP | another name | apCMV-BNC1(human)-EGFP |
| Insert Size (bp) | 2985bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTTGGTACC GAGCTCGGAT CCACTAGTAA CGGCCGCCAG TGTGCTGGAA TTCATGCGGC GGCGCCCGCC GAGCCGGGGC GGACGCGGGG CGGCCCGGGC CCGGGAGACG CGCCGGCAGC CCCGGCACCG CAGCGGTCGC AGGATGGCCG AGGCTATCAG CTGTACTCTG AACTGTAGTT GCCAAAGTTT CAAACCCGGG AAAATAAACC ACCGTCAGTG TGACCAATGC AAGCATGGAT GGGTGGCCCA CGCTCTAAGT AAGCTAAGGA TCCCCCCCAT GTATCCAACA AGCCAGGTGG AGATTGTCCA GTCCAATGTA GTGTTTGATA TTAGCAGCCT CATGCTCTAT GGGACCCAGG CCATCCCCGT TCGCCTAAAA ATCCTACTGG ACCGGCTCTT CAGTGTGTTG AAGCAAGATG AGGTTCTCCA GATCCTCCAT GCCTTGGACT GGACACTTCA GGATTATATC CGTGGATACG TACTGCAGGA TGCATCAGGA AAGGTGTTGG ATCACTGGAG CATCATGACC AGTGAGGAAG AAGTGGCCAC CTTGCAGCAG TTCCTTCGTT TTGGAGAGAC CAAATCTATA GTTGAACTCA TGGCAATTCA AGAGAAAGAA GAGCAATCCA TCATCATACC ACCTTCCACA GCAAATGTAG ATATCAGGGC TTTCATCGAG AGCTGCAGTC ACAGGAGTTC TAGCCTCCCC ACTCCTGTGG ACAAAGGAAA CCCCAGCAGT ATACACCCCT TTGAGAACCT CATAAGCAAC ATGACTTTCA TGCTGCCTTT CCAGTTCTTC AACCCTCTGC CTCCTGCACT GATAGGGTCA TTGCCCGAAC AATATATGTT GGAGCAGGGT CATGACCAAA GTCAGGACCC CAAACAGGAA GTCCATGGGC CCTTCCCTGA CAGCAGCTTC TTAACTTCCA GTTCCACACC ATTTCAGGTT GAAAAAGATC AGTGTTTAAA CTGTCCGGAT GCTATTACTA AAAAAGAAGA CAGCACCCAT TTAAGTGACT CCAGCTCATA CAACATTGTC ACTAAGTTTG AAAGGACACA GTTATCCCCT GAGGCCAAAG TGAAGCCTGA GAGGAATAGC CTTGGTACAA AGAAGGGCCG GGTGTTCTGC ACTGCATGTG AGAAGACCTT CTATGACAAA GGCACCCTCA AAATCCACTA CAATGCCGTC CACTTGAAGA TCAAGCATAA GTGCACCATC GAAGGGTGTA ACATGGTGTT CAGCTCCCTA AGGAGCCGGA ATCGCCATAG CGCCAACCCC AACCCTCGGC TGCACATGCC AATGAACAGA AATAACCGGG ACAAAGACCT CAGGAACAGC CTGAACCTGG CCAGCTCTGA GAACTACAAG TGCCCAGGTT TCACAGTGAC GTCCCCAGAC TGTAGGCCTC CTCCCAGCTA CCCTGGTTCA GGAGAGGATT CCAAAGGCCA ACCAGCCTTC CCAAACATTG GGCAAAATGG TGTGCTTTTT CCCAACCTAA AGACAGTCCA GCCAGTCCTT CCTTTCTACC GCAGTCCAGC CACGCCTGCC GAGGTAGCAA ACACGCCTGG GATACTCCCT TCCCTCCCGC TGTTGTCCTC TTCAATCCCA GAACAGCTCA TTTCAAACGA AATGCCATTT GATGCCCTTC CCAAGAAGAA ATCCAGGAAG TCCAGTATGC CTATCAAAAT AGAGAAAGAA GCTGTGGAAA TAGCTAATGA GAAAAGACAC AACCTCAGCT CAGATGAAGA CATGCCCCTA CAGGTGGTCA GTGAAGATGA GCAGGAGGCC TGCAGTCCTC AGTCACACAG AGTATCTGAG GAGCAGCATG TACAGTCAGG AGGCTTAGGG AAGCCTTTCC CTGAAGGGGA GAGGCCCTGC CATCGTGAAT CAGTAATTGA GTCCAGTGGA GCCATCAGCC AAACCCCTGA GCAGGCCACA CACAATTCAG AGAGGGAGAC TGAGCAGACA CCAGCATTGA TCATGGTGCC AAGGGAGGTC GAGGATGGTG GCCATGAACA CTACTTCACA CCTGGGATGG AACCCCAAGT TCCTTTTTCT GACTACATGG AACTGCAGCA GCGCCTGCTG GCTGGGGGAC TCTTCAGTGC TTTGTCCAAC AGGGGAATGG CTTTTCCTTG TCTTGAAGAT TCTAAAGAAC TGGAGCACGT GGGTCAGCAT GCATTAGCAA GGCAGATAGA AGAAAATCGC TTCCAGTGTG ACATCTGCAA GAAGACCTTT AAAAATGCTT GTAGTGTGAA AATTCATCAC AAGAATATGC ATGTCAAAGA AATGCACACA TGCACAGTGG AGGGCTGTAA TGCTACCTTT CCCTCCCGCA GGAGCAGAGA CAGACACAGC TCAAACCTAA ACCTCCACCA AAAAGCATTG AGCCAGGAAG CATTGGAGAG TAGTGAAGAT CATTTCCGTG CAGCTTACCT TCTGAAAGAT GTGGCTAAGG AAGCCTATCA GGATGTGGCT TTTACACAGC AAGCCTCCCA GACATCTGTC ATCTTCAAAG GAACAAGTCG AATGGGCAGT CTGGTTTACC CAATAACGCA AGTCCACAGT GCCAGCCTGG AGAGCTACAA CTCTGGCCCC TTGAGCGAGG GCACCATCCT GGATTTGAGC ACTACCTCGA GCATGAAGTC AGAGAGTAGC AGCCATTCTT CCTGGGACTC TGACGGGGTG AGTGAGGAAG GCACTGTGCT TATGGAGGAC AGTGATGGGA ACTGTGAAGG GTCGAGCCTT GTCCCTGGGG AAGATGAGTA CCCCATCTGT GTCCTGATGG AGAAGGCTGA CCAGAGCCTT GCTAGCCTGC CTTCTGGGTT GCCCATAACC TGTCATCTCT GCCAAAAGAC ATACAGTAAC AAAGGGACCT TTAGGGCCCA CTACAAAACT GTGCACCTCC GGCAGCTCCA CAAATGCAAA GTACCAGGCT GCAACACCAT GTTTTCGTCT GTTCGCAGTC GAAACAGACA CAGCCAGAAT CCCAACCTGC ACAAAAGCCT GGCCTCATCT CCAAGTCACC TCCAGCTCGA GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAATT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTC
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