pLV2-CMV-IGF1R(human)-EGFP-Puro
$113.00
In stock
SKU
PL40767
NM_000875.5;CD221; IGFIR; IGFR; JTK13
100μg liquid plasmid(Instantly):$134
| name | pLV2-CMV-IGF1R(human)-EGFP-Puro | another name | apLV2-CMV-IGF1R(human)-EGFP-Puro |
| Insert Size (bp) | 4104bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG ACAGCAGAGA TCCAGTTTAT CGATAAGCTT GGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG ACACCGACTC TACTAGAGGA TCGCTAGCGC TACCGGACTC AGATCTCGAG CTCAAGCTTC GAATTCGCCA CCATGAAGTC TGGCTCCGGA GGAGGGTCCC CGACCTCGCT GTGGGGGCTC CTGTTTCTCT CCGCCGCGCT CTCGCTCTGG CCGACGAGTG GAGAAATCTG CGGGCCAGGC ATCGACATCC GCAACGACTA TCAGCAGCTG AAGCGCCTGG AGAACTGCAC GGTGATCGAG GGCTACCTCC ACATCCTGCT CATCTCCAAG GCCGAGGACT ACCGCAGCTA CCGCTTCCCC AAGCTCACGG TCATTACCGA GTACTTGCTG CTGTTCCGAG TGGCTGGCCT CGAGAGCCTC GGAGACCTCT TCCCCAACCT CACGGTCATC CGCGGCTGGA AACTCTTCTA CAACTACGCC CTGGTCATCT TCGAGATGAC CAATCTCAAG GATATTGGGC TTTACAACCT GAGGAACATT ACTCGGGGGG CCATCAGGAT TGAGAAAAAT GCTGACCTCT GTTACCTCTC CACTGTGGAC TGGTCCCTGA TCCTGGATGC GGTGTCCAAT AACTACATTG TGGGGAATAA GCCCCCAAAG GAATGTGGGG ACCTGTGTCC AGGGACCATG GAGGAGAAGC CGATGTGTGA GAAGACCACC ATCAACAATG AGTACAACTA CCGCTGCTGG ACCACAAACC GCTGCCAGAA AATGTGCCCA AGCACGTGTG GGAAGCGGGC GTGCACCGAG AACAATGAGT GCTGCCACCC CGAGTGCCTG GGCAGCTGCA GCGCGCCTGA CAACGACACG GCCTGTGTAG CTTGCCGCCA CTACTACTAT GCCGGTGTCT GTGTGCCTGC CTGCCCGCCC AACACCTACA GGTTTGAGGG CTGGCGCTGT GTGGACCGTG ACTTCTGCGC CAACATCCTC AGCGCCGAGA GCAGCGACTC CGAGGGGTTT GTGATCCACG ACGGCGAGTG CATGCAGGAG TGCCCCTCGG GCTTCATCCG CAACGGCAGC CAGAGCATGT ACTGCATCCC TTGTGAAGGT CCTTGCCCGA AGGTCTGTGA GGAAGAAAAG AAAACAAAGA CCATTGATTC TGTTACTTCT GCTCAGATGC TCCAAGGATG CACCATCTTC AAGGGCAATT TGCTCATTAA CATCCGACGG GGGAATAACA TTGCTTCAGA GCTGGAGAAC TTCATGGGGC TCATCGAGGT GGTGACGGGC TACGTGAAGA TCCGCCATTC TCATGCCTTG GTCTCCTTGT CCTTCCTAAA AAACCTTCGC CTCATCCTAG GAGAGGAGCA GCTAGAAGGG AATTACTCCT TCTACGTCCT CGACAACCAG AACTTGCAGC AACTGTGGGA CTGGGACCAC CGCAACCTGA CCATCAAAGC AGGGAAAATG TACTTTGCTT TCAATCCCAA ATTATGTGTT TCCGAAATTT ACCGCATGGA GGAAGTGACG GGGACTAAAG GGCGCCAAAG CAAAGGGGAC ATAAACACCA GGAACAACGG GGAGAGAGCC TCCTGTGAAA GTGACGTCCT GCATTTCACC TCCACCACCA CGTCGAAGAA TCGCATCATC ATAACCTGGC ACCGGTACCG GCCCCCTGAC TACAGGGATC TCATCAGCTT CACCGTTTAC TACAAGGAAG CACCCTTTAA GAATGTCACA GAGTATGATG GGCAGGATGC CTGCGGCTCC AACAGCTGGA ACATGGTGGA CGTGGACCTC CCGCCCAACA AGGACGTGGA GCCCGGCATC TTACTACATG GGCTGAAGCC CTGGACTCAG TACGCCGTTT ACGTCAAGGC TGTGACCCTC ACCATGGTGG AGAACGACCA TATCCGTGGG GCCAAGAGTG AGATCTTGTA CATTCGCACC AATGCTTCAG TTCCTTCCAT TCCCTTGGAC GTTCTTTCAG CATCGAACTC CTCTTCTCAG TTAATCGTGA AGTGGAACCC TCCCTCTCTG CCCAACGGCA ACCTGAGTTA CTACATTGTG CGCTGGCAGC GGCAGCCTCA GGACGGCTAC CTTTACCGGC ACAATTACTG CTCCAAAGAC AAAATCCCCA TCAGGAAGTA TGCCGACGGC ACCATCGACA TTGAGGAGGT CACAGAGAAC CCCAAGACTG AGGTGTGTGG TGGGGAGAAA GGGCCTTGCT GCGCCTGCCC CAAAACTGAA GCCGAGAAGC AGGCCGAGAA GGAGGAGGCT GAATACCGCA AAGTCTTTGA GAATTTCCTG CACAACTCCA TCTTCGTGCC CAGACCTGAA AGGAAGCGGA GAGATGTCAT GCAAGTGGCC AACACCACCA TGTCCAGCCG AAGCAGGAAC ACCACGGCCG CAGACACCTA CAACATCACC GACCCGGAAG AGCTGGAGAC AGAGTACCCT TTCTTTGAGA GCAGAGTGGA TAACAAGGAG AGAACTGTCA TTTCTAACCT TCGGCCTTTC ACATTGTACC GCATCGATAT CCACAGCTGC AACCACGAGG CTGAGAAGCT GGGCTGCAGC GCCTCCAACT TCGTCTTTGC AAGGACTATG CCCGCAGAAG GAGCAGATGA CATTCCTGGG CCAGTGACCT GGGAGCCAAG GCCTGAAAAC TCCATCTTTT TAAAGTGGCC GGAACCTGAG AATCCCAATG GATTGATTCT AATGTATGAA ATAAAATACG GATCACAAGT TGAGGATCAG CGAGAATGTG TGTCCAGACA GGAATACAGG AAGTATGGAG GGGCCAAGCT AAACCGGCTA AACCCGGGGA ACTACACAGC CCGGATTCAG GCCACATCTC TCTCTGGGAA TGGGTCGTGG ACAGATCCTG TGTTCTTCTA TGTCCAGGCC AAAACAGGAT ATGAAAACTT CATCCATCTG ATCATCGCTC TGCCCGTCGC TGTCCTGTTG ATCGTGGGAG GGTTGGTGAT TATGCTGTAC GTCTTCCATA GAAAGAGAAA TAACAGCAGG CTGGGGAATG GAGTGCTGTA TGCCTCTGTG AACCCGGAGT ACTTCAGCGC TGCTGATGTG TACGTTCCTG ATGAGTGGGA GGTGGCTCGG GAGAAGATCA CCATGAGCCG GGAACTTGGG CAGGGGTCGT TTGGGATGGT CTATGAAGGA GTTGCCAAGG GTGTGGTGAA AGATGAACCT GAAACCAGAG TGGCCATTAA AACAGTGAAC GAGGCCGCAA GCATGCGTGA GAGGATTGAG TTTCTCAACG AAGCTTCTGT GATGAAGGAG TTCAATTGTC ACCATGTGGT GCGATTGCTG GGTGTGGTGT CCCAAGGCCA GCCAACACTG GTCATCATGG AACTGATGAC ACGGGGCGAT CTCAAAAGTT ATCTCCGGTC TCTGAGGCCA GAAATGGAGA ATAATCCAGT CCTAGCACCT CCAAGCCTGA GCAAGATGAT TCAGATGGCC GGAGAGATTG CAGACGGCAT GGCATACCTC AACGCCAATA AGTTCGTCCA CAGAGACCTT GCTGCCCGGA ATTGCATGGT AGCCGAAGAT TTCACAGTCA AAATCGGAGA TTTTGGTATG ACGCGAGATA TCTATGAGAC AGACTATTAC CGGAAAGGAG GGAAAGGGCT GCTGCCCGTG CGCTGGATGT CTCCTGAGTC CCTCAAGGAT GGAGTCTTCA CCACTTACTC GGACGTCTGG TCCTTCGGGG TCGTCCTCTG GGAGATCGCC ACACTGGCCG AGCAGCCCTA CCAGGGCTTG TCCAACGAGC AAGTCCTTCG CTTCGTCATG GAGGGCGGCC TTCTGGACAA GCCAGACAAC TGTCCTGACA TGCTGTTTGA ACTGATGCGC ATGTGCTGGC AGTATAACCC CAAGATGAGG CCTTCCTTCC TGGAGATCAT CAGCAGCATC AAAGAGGAGA TGGAGCCTGG CTTCCGGGAG GTCTCCTTCT ACTACAGCGA GGAGAACAAG CTGCCCGAGC CGGAGGAGCT GGACCTGGAG CCAGAGAACA TGGAGAGCGT CCCCCTGGAC CCCTCGGCCT CCTCGTCCTC CCTGCCACTG CCCGACAGAC ACTCAGGACA CAAGGCCGAG AACGGCCCCG GCCCTGGGGT GCTGGTCCTC CGCGCCAGCT TCGACGAGAG ACAGCCTTAC GCCCACATGA ACGGGGGCCG CAAGAACGAG CGGGCCTTGC CGCTGCCCCA GTCTTCGACC TGCGGCGGCG GAGGGAGTGG AGGAGGGGGA AGCGGATCCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAGT CTAGATAATT CTACCGGGTA GGGGAGGCGC TTTTCCCAAG GCAGTCTGGA GCATGCGCTT TAGCAGCCCC GCTGGGCACT TGGCGCTACA CAAGTGGCCT CTGGCCTCGC ACACATTCCA CATCCACCGG TAGGCGCCAA CCGGCTCCGT TCTTTGGTGG CCCCTTCGCG CCACCTTCTA CTCCTCCCCT AGTCAGGAAG TTCCCCCCCG CCCCGCAGCT CGCGTCGTGC AGGACGTGAC AAATGGAAGT AGCACGTCTC ACTAGTCTCG TGCAGATGGA CAGCACCGCT GAGCAATGGA AGCGGGTAGG CCTTTGGGGC AGCGGCCAAT AGCAGCTTTG CTCCTTCGCT TTCTGGGCTC AGAGGCTGGG AAGGGGTGGG TCCGGGGGCG GGCTCAGGGG CGGGCTCAGG GGCGGGGCGG GCGCCCGAAG GTCCTCCGGA GGCCCGGCAT TCTGCACGCT TCAAAAGCGC ACGTCTGCCG CGCTGTTCTC CTCTTCCTCA TCTCCGGGCC TTTCGACCTG CAGCCCAAGC TTACCATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGACCGCG TCTGGAACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GAATTAATTC TGCAGTCGAG ACCTAGAAAA ACATGGAGCA ATCACAAGTA GCAATACAGC AGCTACCAAT GCTGATTGTG CCTGGCTAGA AGCACAAGAG GAGGAGGAGG TGGGTTTTCC AGTCACACCT CAGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGAGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA GACAAGATAT CCTTGATCTG TGGATCTACC ACACACAAGG CTACTTCCCT GATTAGCAGA ACTACACACC AGGGCCAGGG GTCAGATATC CACTGACCTT TGGATGGTGC TACAAGCTAG TACCAGTTGA GCCAGATAAG GTAGAAGAGG CCAATAAAGG AGAGAACACC AGCTTGTTAC ACCCTGTGAG CCTGCATGGG ATGGATGACC CGGAGAGAGA AGTGTTAGAG TGGAGGTTTG ACAGCCGCCT AGCATTTCAT CACGTGGCCC GAGAGCTGCA TCCGGAGTAC TTCAAGAACT GCTGATATCG AGCTTGCTAC AAGGGACTTT CCGCTGGGGA CTTTCCAGGG AGGCGTGGCC TGGGCGGGAC TGGGGAGTGG CGAGCCCTCA GATCCTGCAT ATAAGCAGCT GCTTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTA GTAGTTCATG TCATCTTATT ATTCAGTATT TATAACTTGC AAAGAAATGA ATATCAGAGA GTGAGAGGCC TTGACATTGC TAGCGTTTTA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCAACTGG ATAACTCAAG CTAACCAAAA TCATCCCAAA CTTCCCACCC CATACCCTAT TACCACTGCC AATTACCTGT GGTTTCATTT ACTCTAAACC TGTGATTCCT CTGAATTATT TTCATTTTAA AGAAATTGTA TTTGTTAAAT ATGTACTACA AACTTAGTAG TTTTTAAAGA AATTGTATTT GTTAAATATG TACTACAAAC TTAGTAGT
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