pSV40-ISG20(human)-5'UTR-mut-Rluc-FLAG-HSVTK-Fluc
$70.00
In stock
SKU
PL40701
100μg liquid plasmid(Instantly):$91
| name | pSV40-ISG20(human)-5'UTR-mut-Rluc-FLAG-HSVTK-Fluc | another name | apSV40-ISG20(human)-5'UTR-mut-Rluc-FLAG-HSVTK-Fluc |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | UTR |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | SV40 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC ACTTTGCCTT TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA GTACTTAATA CGACTCACTA TAGGCTAGCG AGCGCAGAAG CAGGCAGATG AGAGCAGGCC ACCATGGCTT CCAAGGTGTA CGACCCCGAG CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT CTGCATGGTA ACGCTGCCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGG CTTGTCTGGC CTTTCACTAC TCCTACGAGC ACCAAGACAA GATCAAGGCC ATCGTCCATG CTGAGAGTGT CGTGGACGTG ATCGAGTCCT GGGACGAGTG GCCTGACATC GAGGAGGATA TCGCCCTGAT CAAGAGCGAA GAGGGCGAGA AAATGGTGCT TGAGAATAAC TTCTTCGTCG AGACCATGCT CCCAAGCAAG ATCATGCGGA AACTGGAGCC TGAGGAGTTC GCTGCCTACC TGGAGCCATT CAAGGAGAAG GGCGAGGTTA GACGGCCTAC CCTCTCCTGG CCTCGCGAGA TCCCTCTCGT TAAGGGAGGC AAGCCCGACG TCGTCCAGAT TGTCCGCAAC TACAACGCCT ACCTTCGGGC CAGCGACGAT CTGCCTAAGA TGTTCATCGA GTCCGACCCT GGGTTCTTTT CCAACGCTAT TGTCGAGGGA GCTAAGAAGT TCCCTAACAC CGAGTTCGTG AAGGTGAAGG GCCTCCACTT CAGCCAGGAG GACGCTCCAG ATGAAATGGG TAAGTACATC AAGAGCTTCG TGGAGCGCGT GCTGAAGAAC GAGCAGGATT ACAAGGACGA CGATGACAAG TAACTCGAGC CCGGGAATTC GTTTAAACCT AGAGCGGCCG CTGGCCGCAA TAAAATATCT TTATTTTCAT TACATCTGTG TGTTGGTTTT TTGTGTGAGG ATCTAAATGA GTCTTCGGAC CTCGCGGGGG CCGCTTAAGC GGTGGTTAGG GTTTGTCTGA CGCGGGGGGA GGGGGAAGGA ACGAAACACT CTCATTCGGA GGCGGCTCGG GGTTTGGTCT TGGTGGCCAC GGGCACGCAG AAGAGCGCCG CGATCCTCTT AAGCACCCCC CCGCCCTCCG TGGAGGCGGG GGTTTGGTCG GCGGGTGGTA ACTGGCGGGC CGCTGACTCG GGCGGGTCGC GCGCCCCAGA GTGTGACCTT TTCGGTCTGC TCGCAGACCC CCGGGCGGCG CCGCCGCGGC GGCGACGGGC TCGCTGGGTC CTAGGCTCCA TGGGGACCGT ATACGTGGAC AGGCTCTGGA GCATCCGCAC GACTGCGGTG ATATTACCGG AGACCTTCTG CGGGACGAGC CGGGTCACGC GGCTGACGCG GAGCGTCCGT TGGGCGACAA ACACCAGGAC GGGGCACAGG TACACTATCT TGTCACCCGG AGGCGCGAGG GACTGCAGGA GCTTCAGGGA GTGGCGCAGC TGCTTCATCC CCGTGGCCCG TTGCTCGCGT TTGCTGGCGG TGTCCCCGGA AGAAATATAT TTGCATGTCT TTAGTTCTAT GATGACACAA ACCCCGCCCA GCGTCTTGTC ATTGGCGAAT TCGAACACGC AGATGCAGTC GGGGCGGCGC GGTCCCAGGT CCACTTCGCA TATTAAGGTG ACGCGTGTGG CCTCGAACAC CGAGCGACCC TGCAGCGACC CGCTTAAAAG CTTGGCATTC CGGTACTGTT GGTAAAGCCA CCATGGCCGA TGCTAAGAAC ATTAAGAAGG GCCCTGCTCC CTTCTACCCT CTGGAGGATG GCACCGCTGG CGAGCAGCTG CACAAGGCCA TGAAGAGGTA TGCCCTGGTG CCTGGCACCA TTGCCTTCAC CGATGCCCAC ATTGAGGTGG ACATCACCTA TGCCGAGTAC TTCGAGATGT CTGTGCGCCT GGCCGAGGCC ATGAAGAGGT ACGGCCTGAA CACCAACCAC CGCATCGTGG TGTGCTCTGA GAACTCTCTG CAGTTCTTCA TGCCAGTGCT GGGCGCCCTG TTCATCGGAG TGGCCGTGGC CCCTGCTAAC GACATTTACA ACGAGCGCGA GCTGCTGAAC AGCATGGGCA TTTCTCAGCC TACCGTGGTG TTCGTGTCTA AGAAGGGCCT GCAGAAGATC CTGAACGTGC AGAAGAAGCT GCCTATCATC CAGAAGATCA TCATCATGGA CTCTAAGACC GACTACCAGG GCTTCCAGAG CATGTACACA TTCGTGACAT CTCATCTGCC TCCTGGCTTC AACGAGTACG ACTTCGTGCC AGAGTCTTTC GACAGGGACA AAACCATTGC CCTGATCATG AACAGCTCTG GGTCTACCGG CCTGCCTAAG GGCGTGGCCC TGCCTCATCG CACCGCCTGT GTGCGCTTCT CTCACGCCCG CGACCCTATT TTCGGCAACC AGATCATCCC CGACACCGCT ATTCTGAGCG TGGTGCCATT CCACCACGGC TTCGGCATGT TCACCACCCT GGGCTACCTG ATTTGCGGCT TTCGGGTGGT GCTGATGTAC CGCTTCGAGG AGGAGCTGTT CCTGCGCAGC CTGCAAGACT ACAAAATTCA GTCTGCCCTG CTGGTGCCAA CCCTGTTCAG CTTCTTCGCT AAGAGCACCC TGATCGACAA GTACGACCTG TCTAACCTGC ACGAGATTGC CTCTGGCGGC GCCCCACTGT CTAAGGAGGT GGGCGAAGCC GTGGCCAAGC GCTTTCATCT GCCAGGCATC CGCCAGGGCT ACGGCCTGAC CGAGACAACC AGCGCCATTC TGATTACCCC AGAGGGCGAC GACAAGCCTG GCGCCGTGGG CAAGGTGGTG CCATTCTTCG AGGCCAAGGT GGTGGACCTG GACACCGGCA AGACCCTGGG AGTGAACCAG CGCGGCGAGC TGTGTGTGCG CGGCCCTATG ATTATGTCCG GCTACGTGAA TAACCCTGAG GCCACAAACG CCCTGATCGA CAAGGACGGC TGGCTGCACT CTGGCGACAT TGCCTACTGG GACGAGGACG AGCACTTCTT CATCGTGGAC CGCCTGAAGT CTCTGATCAA GTACAAGGGC TACCAGGTGG CCCCAGCCGA GCTGGAGTCT ATCCTGCTGC AGCACCCTAA CATTTTCGAC GCCGGAGTGG CCGGCCTGCC CGACGACGAT GCCGGCGAGC TGCCTGCCGC CGTCGTCGTG CTGGAACACG GCAAGACCAT GACCGAGAAG GAGATCGTGG ACTATGTGGC CAGCCAGGTG ACAACCGCCA AGAAGCTGCG CGGCGGAGTG GTGTTCGTGG ACGAGGTGCC CAAGGGCCTG ACCGGCAAGC TGGACGCCCG CAAGATCCGC GAGATCCTGA TCAAGGCTAA GAAAGGCGGC AAGATCGCCG TGTAATAATT CTAGAGTCGG GGCGGCCGGC CGCTTCGAGC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT AAAATCGATA AGGATCCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGGCT CGAC
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