pLVX-TetOne-TRE3GS-hTERT-Neo
$113.00
In stock
SKU
PL40566
100μg liquid plasmid(Instantly):$134
| name | pLVX-TetOne-TRE3GS-hTERT-Neo | another name | apLVX-TetOne-TRE3GS-hTERT-Neo |
| Insert Size (bp) | 3399bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | TRE3GS | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTACTAAGT TTGTAGTACA TATTTAACAA ATACAATTTC TTTAAAAACT ACTAAGTTTG TAGTACATAT TTAACAAATA CAATTTCTTT AAAATGAAAA TAATTCAGAG GAATCACAGG TTTAGAGTAA ATGAAACCAC TAGGTAATTG GCAGTGGTAA TAGGGTATGG GGTGGGAAGT TTGGGATGAT TTTGGTTAGC TTGAGTTATC CAGTTGATCC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT GATCTCCCGA TCCGTCGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCT AGCTAGAGGT CGACGGTAAA ACGCTAGCAA TGTCAAGGCC TCTCACTCTC TGATATTCAT TTCTTTGCAA GTTATAAATA CTGAATAATA AGATGACATG AACTACTACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGGCA AAAAGCAGCT GCTTATATGC AGGATCTGAG GGCTCGCCAC TCCCCAGTCC CGCCCAGGCC ACGCCTCCCT GGAAAGTCCC CAGCGGAAAG TCCCTTGTAG CAAGCTCGAT ATCAGCAGTT CTTGAAGTAC TCCGGATGCA GCTCTCGGGC CACGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AACACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCTGGTG TTCTCTCCTT TATTGGCCTC TTCTACCTTA TCTGGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGACCCCT GGCCCTGGTG TGTAGTTCTG CTAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCT CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC GACTGCAGAA TTAATTCCAG GCGGGGAGGC GGCCCAAAGG GAGATCCGAC TCGTCTGAGG GCGAAGGCGA AGACGCGGAA GAGGCCGCAG AGCCGGCAGC AGGCCGCGGG AAGGAAGGTC CGCTGGATTG AGGGCCGAAG GGACGTAGCA GAAGGACGTC CCGCGCAGAA TCCAGGTGGC AACACAGGCG AGCAGCCATG GAAAGGACGT CAGCTTCCCC GACAACACCA CGGAATTGTC AGTGCCCAAC AGCCGAGCCC CTGTCCAGCA GCGGGCAAGG CAGGCGGCGA TGAGTTCCGC CGTGGCAATA GGGAGGGGGA AAGCGAAAGT CCCGGAAAGG AGCTGACAGG TGGTGGCAAT GCCCCAACCA GTGGGGGTTG CGTCAGCAAA CACAGTGCAC ACCACGCCAC GTTGCCTGAC AACGGGCCAC AACTCCTCAT AAAGAGACAG CAACCAGGAT TTATACAAGG AGGAGAAAAT GAAAGCCATA CGGGAAGCAA TAGCATGATA CAAAGGCATT AAAGCAGCGT ATCCACATAG CGTAAAAGGA GCAACATAGT TAAGAATACC AGTCAATCTT TCACAAATTT TGTAATCCAG AGGTTGATTG TTCCAGACGC GTTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGG TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCGC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCCTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAGCCATGGT CGCGTGTAGG CGCCGGTCAC AGCTTGGATC TGTAACGGCG CAGAACAGAA AACGAAACAA AGACGTAGAG TTGAGCAAGC AGGGTCAGGC AAAGCGTGGA GAGCCGGCTG AGTCTAGGTA GGCTCCAAGG GAGCGCCGGA CAAAGGCCCG GTCTCGACCT GAGCTTTAAA CTTACCTAGA CGGCGGACGC AGTTCAGGAG GCACCACAGG CGGGAGGCGG CAGAACGCGA CTCAACCGGC GTGGATGGCG GCCTCAGGTA GGGCGGCGGG CGCGTGAAGG AGAGATGCGA GCCCCTCGAA GCTTCAGCTG TGTTCTGGCG GCAAACCCGT TGCGAAAAAG AACGTTCACG GCGACTACTG CACTTATATA CGGTTCTCCC CCACCCTCGG GAAAAAGGCG GAGCCAGTAC ACGACATCAC TTTCCCAGTT TACCCCGCGC CACCTTCTCT AGGCACCCGT TCAATTGCCG ACCCCTCCCC CCAACTTCTC GGGGACTGTG GGCGATGTGC GCTCTGCCCA CTGACGGGCA CCGGAGCGAT CGCAGATCCT TATTCGCGCG TTTACCCGGG GAGCATGTCA AGGTCAAAAT CGTCAAGAGC GTCAGCAGGC AGCATATCAA GGTCAAAGTC GTCAAGGGCA TCGGCTGGGA GCATGTCTAA GTCAAAATCG TCAAGGGCGT CGGTCGGCCC GCCGCTTTCG CACTTTAGCT GTTTCTCCAG GCCACATATG ATTAGTTCCA GGCCGAAAAG GAAGGCAGGT TCGGCTCCCT GCCGGTCGAA CAGCTCAATT GCTTGTTTCA GAAGTGGGGG CATAGAATCG GTGGTAGGTG TCTCTCTTTC CTCTTTTGCT ACTTGATGCT CCTGTTCCTC CAATACGCAG CCCAGTGTAA AGTGGCCCAC GGCGGACAGA GCGTACAGTG CGTTCTCCAG GGAGAAGCCT TGCTGACACA GGAACGCGAG CTGATTTTCC AGGGTTTCGT ACTGTTTCTC TGTTGGGCGG GTGCCGAGAT GCACTTTAGC CCCGTCGCGA TGTGAGAGGA GAGCACAGCG GTATGACTTG GCGTTGTTCC GCAGAAAGTC TTGCCATGAC TCGCCTTCCA GGGGGCAGGA GTGGGTATGA TGCCTGTCCA GCATCTCGAT TGGCAGGGCA TCGAGCAGGG CCCGCTTGTT CTTCACGTGC CAGTACAGGG TAGGCTGCTC AACTCCCAGC TTTTGAGCGA GTTTCCTTGT CGTCAGGCCT TCGATACCGA CTCCATTGAG TAATTCCAGA GCAGAGTTTA TGACTTTGCT CTTGTCCAGT CTAGACATGG TAATTCGATG ATCCCCCTGG GGAGAGAGGT CGGTGATTCG GTCAACGAGG GAGCCGACTG CCGACGTGCG CTCCGGAGGC TTGCAGAATG CGGAACACCG CGCGGGCAGG AACAGGGCCC ACACTACCGC CCCACACCCC GCCTCCCGCA CCGCCCCTTC CCGGCCGCTG CTCTCGGCGC GCCCTGCTGA GCAGCCGCTA TTGGCCACAG CCCATCGCGG TCGGCGCGCT GCCATTGCTC CCTGGCGCTG TCCGTCTGCG AGGGTACTAG TGAGACGTGC GGCTTCCGTT TGTCACGTCC GGCACGCCGC GAACCGCAAG GAACCTTCCC GACTTAGGGG CGGAGCAGGA AGCGTCGCCG GGGGGCCCAC AAGGGTAGCG GCGAAGATCC GGGTGACGCT GCGAACGGAC GTGAAGAATG TGCGAGACCC AGGGTCGGCG CCGCTGCGTT TCCCGGAACC ACGCCCAGAG CAGCCGCGTC CCTGCGCAAA CCCAGGGCTG CCTTGGAAAA GGCGCAACCC CAACCCCGTG GAATTATCAC CTCGAGTTTA CTCCCTATCA GTGATAGAGA ACGTATGAAG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGCAGACTT TACTCCCTAT CAGTGATAGA GAACGTATAA GGAGTTTACT CCCTATCAGT GATAGAGAAC GTATGACCAG TTTACTCCCT ATCAGTGATA GAGAACGTAT CTACAGTTTA CTCCCTATCA GTGATAGAGA ACGTATATCC AGTTTACTCC CTATCAGTGA TAGAGAACGT ATAAGCTTTG CTTATGTAAA CCAGGGCGCC TATAAAAGAG TGCTGATTTT TTGAGTAAAC TTCAATTCCA CAACACTTTT GTCTTATACC AACTTTCCGT ACCACTTCCT ACCCTCGTAA AGAATTCGCC ACCATGCCGC GCGCTCCCCG CTGCCGAGCC GTGCGCTCCC TGCTGCGCAG CCACTACCGC GAGGTGCTGC CGCTGGCCAC GTTCGTGCGG CGCCTGGGGC CCCAGGGCTG GCGGCTGGTG CAGCGCGGGG ACCCGGCGGC TTTCCGCGCG CTGGTGGCCC AGTGCCTGGT GTGCGTGCCC TGGGACGCAC GGCCGCCCCC CGCCGCCCCC TCCTTCCGCC AGGTGTCCTG CCTGAAGGAG CTGGTGGCCC GAGTGCTGCA GAGGCTGTGC GAGCGCGGCG CGAAGAACGT GCTGGCCTTC GGCTTCGCGC TGCTGGACGG GGCCCGCGGG GGCCCCCCCG AGGCCTTCAC CACCAGCGTG CGCAGCTACC TGCCCAACAC GGTGACCGAC GCACTGCGGG GGAGCGGGGC GTGGGGGCTG CTGCTGCGCC GCGTGGGCGA CGACGTGCTG GTTCACCTGC TGGCACGCTG CGCGCTCTTT GTGCTGGTGG CTCCCAGCTG CGCCTACCAG GTGTGCGGGC CGCCGCTGTA CCAGCTCGGC GCTGCCACTC AGGCCCGGCC CCCGCCACAC GCTAGTGGAC CCCGAAGGCG TCTGGGATGC GAACGGGCCT GGAACCATAG CGTCAGGGAG GCCGGGGTCC CCCTGGGCCT GCCAGCCCCG GGTGCGAGGA GGCGCGGGGG CAGTGCCAGC CGAAGTCTGC CGTTGCCCAA GAGGCCCAGG CGTGGCGCTG CCCCTGAGCC GGAGCGGACG CCCGTTGGGC AGGGGTCCTG GGCCCACCCG GGCAGGACGC GTGGACCGAG TGACCGTGGT TTCTGTGTGG TGTCACCTGC CAGACCCGCC GAAGAAGCCA CCTCTTTGGA GGGTGCGCTC TCTGGCACGC GCCACTCCCA CCCATCCGTG GGCCGCCAGC ACCACGCGGG CCCCCCATCC ACATCGCGGC CACCACGTCC CTGGGACACG CCTTGTCCCC CGGTGTACGC CGAGACCAAG CACTTCCTCT ACTCCTCAGG CGACAAGGAG CAGCTGCGGC CCTCCTTCCT ACTCAGCTCT CTGAGGCCCA GCCTGACTGG CGCTCGGAGG CTCGTGGAGA CCATCTTTCT GGGTTCCAGG CCCTGGATGC CAGGGACTCC CCGCAGGTTG CCCCGCCTGC CCCAGCGCTA CTGGCAAATG CGGCCCCTGT TTCTGGAGCT GCTTGGGAAC CACGCGCAGT GCCCCTACGG GGTGCTCCTC AAGACGCACT GCCCGCTGCG AGCTGCGGTC ACCCCAGCAG CCGGTGTCTG TGCCCGGGAG AAGCCCCAGG GCTCTGTGGC GGCCCCCGAG GAGGAGGACA CAGACCCCCG TCGCCTGGTG CAGCTGCTCC GCCAGCACAG CAGCCCCTGG CAGGTGTACG GCTTCGTGCG GGCCTGCCTG CGCCGGCTGG TGCCCCCAGG CCTCTGGGGC TCCAGGCACA ACGAACGCCG CTTCCTCAGG AACACCAAGA AGTTCATCTC CCTGGGGAAG CATGCCAAGC TCTCGCTGCA GGAGCTGACG TGGAAGATGA GCGTGCGGGG CTGCGCTTGG CTGCGCAGGA GCCCAGGGGT TGGCTGTGTT CCGGCCGCAG AGCACCGTCT GCGTGAGGAG ATCCTGGCCA AGTTCCTGCA CTGGCTGATG AGTGTGTACG TCGTCGAGCT GCTCAGGTCT TTCTTTTATG TCACGGAGAC CACGTTTCAA AAGAACAGGC TCTTTTTCTA CCGGAAGAGT GTCTGGAGCA AGTTGCAAAG CATTGGAATC AGACAGCACT TGAAGAGGGT GCAGCTGCGG GAGCTGTCGG AAGCAGAGGT CAGGCAGCAT CGGGAAGCCA GGCCCGCCCT GCTGACGTCC AGACTCCGCT TCATCCCCAA GCCTGACGGG CTGCGGCCGA TTGTGAACAT GGACTACGTC GTGGGAGCCA GAACGTTCCG CAGAGAAAAG AGGGCCGAGC GTCTCACCTC GAGGGTGAAG GCACTGTTCA GCGTGCTCAA CTACGAGCGG GCGCGGCGCC CCGGCCTCCT GGGCGCCTCT GTGCTGGGCC TGGACGATAT CCACAGGGCC TGGCGCACCT TCGTGCTGCG TGTGCGGGCC CAGGACCCGC CGCCTGAGCT GTACTTTGTC AAGGTGGATG TGACGGGCGC GTACGACACC ATCCCCCAGG ACAGGCTCAC GGAGGTCATC GCCAGCATCA TCAAACCCCA GAACACGTAC TGCGTGCGTC GGTATGCCGT GGTCCAGAAG GCCGCCCATG GGCACGTCCG CAAGGCCTTC AAGAGCCACG TCTCTACCTT GACAGACCTC CAGCCGTACA TGCGACAGTT CGTGGCTCAC CTGCAGGAGA CCAGCCCGCT GAGGGATGCC GTCGTCATCG AGCAGAGCTC CTCCCTGAAT GAGGCCAGCA GTGGCCTCTT CGACGTCTTC CTACGCTTCA TGTGCCACCA CGCCGTGCGC ATCAGGGGCA AGTCCTACGT CCAGTGCCAG GGGATCCCGC AGGGCTCCAT CCTCTCCACG CTGCTCTGCA GCCTGTGCTA CGGCGACATG GAGAACAAGC TGTTTGCGGG GATTCGGCGG GACGGGCTGC TCCTGCGTTT GGTGGATGAT TTCTTGTTGG TGACACCTCA CCTCACCCAC GCGAAAACCT TCCTCAGGAC CCTGGTCCGA GGTGTCCCTG AGTATGGCTG CGTGGTGAAC TTGCGGAAGA CAGTGGTGAA CTTCCCTGTA GAAGACGAGG CCCTGGGTGG CACGGCTTTT GTTCAGATGC CGGCCCACGG CCTATTCCCC TGGTGCGGCC TGCTGCTGGA TACCCGGACC CTGGAGGTGC AGAGCGACTA CTCCAGCTAT GCCCGGACCT CCATCAGAGC CAGTCTCACC TTCAACCGCG GCTTCAAGGC TGGGAGGAAC ATGCGTCGCA AACTCTTTGG GGTCTTGCGG CTGAAGTGTC ACAGCCTGTT TCTGGATTTG CAGGTGAACA GCCTCCAGAC GGTGTGCACC AACATCTACA AGATCCTCCT GCTGCAGGCG TACAGGTTTC ACGCATGTGT GCTGCAGCTC CCATTTCATC AGCAAGTTTG GAAGAACCCC ACATTTTTCC TGCGCGTCAT CTCTGACACG GCCTCCCTCT GCTACTCCAT CCTGAAAGCC AAGAACGCAG GGATGTCGCT GGGGGCCAAG GGCGCCGCCG GCCCTCTGCC CTCCGAGGCC GTGCAGTGGC TGTGCCACCA AGCATTCCTG CTCAAGCTGA CTCGACACCG TGTCACCTAC GTGCCACTCC TGGGGTCACT CAGGACAGCC CAGACGCAGC TGAGTCGGAA GCTCCCGGGG ACGACGCTGA CTGCCCTGGA GGCCGCAGCC AACCCGGCAC TGCCCTCAGA CTTCAAGACC ATCCTGGACT GAGGATCCAG ACCACCTCCC CTGCGAGCTA AGCTGGACAG CCAATGACGG GTAAGAGAGT GACATTTTTC ACTAACCTAA GACAGGAGGG CCGTCAGAGC TACTGCCTAA TCCAAAGACG GGTAAAAGTG ATAAAAATGT ATCACTCCAA CCTAAGACAG GCGCAGCTTC CGAGGGATTT GAGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCAAAACT AGAATGCAGT GAAAAAAATG CCTTATTTGT GAAATTTGTG ATGCTATTGC CTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAG TCGATAAACT GGATCTCTGC TGTCCCTGTA ATAAACCCGA AAATTTTGAA TTTTTGTAAT TTGTTTTTGT AGTTCTTTAG TTTGTATGTC TGTTGCTATT ATGTCTACTA TTCTTTCCCC TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAGGCG ATACCGTCGA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG TGTAATTGTT AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA GAGATTTATT ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG AGCAACCCCA AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCACA GCCAGGATTC TTGCCTGGAG CTGCTTGATG CCCCAGACTG TGAGTTGCAA CAGATGCTGT TGCGCCTCAA TAGCCCTCAG CAAATTGTTC TGCTGCTGCA CTATACCAGA CAATAATTGT CTGGCCTGTA CCGTCAGCGT CATTGACGCT GCGCCCATAG TGCTTCCTGC TGCTCCCAAG AACCCAAGGA ACAAAGCTCC TATTCCCACT GCTCTTTTTT CTCTCTGCAC CACTCTTCTC TTTGCCTTGG TGGGTGCTAC TCCTAATGGT TCAATTTTTA CTACTTTATA TTTATATAAT TCACTTCTCC AATTGTCCCT CATATCTCCT CCTCCAGGTC TGAAGATCAG CGGCCGGCCG CTTGCTGTGC GGTGGTCTTA CTTTTGTTTT GCTCTTCCTC TATCTTGTCT AAAGCTTCCT TGGTGTCTTT TATCTCTATC CTTTGATGCA CACAATAGAG GGTTGCTACT GTATTATATA ATGATCTAAG TTCTTCTGAT CCTGTCTGAA GGGATGGTTG TAGCTGTCCC AGTATTTGTC TACAGCCTTC TGATGTTTCT AACAGGCCAG GATTAACTGC GAATCGTTCT AGCTCCCTGC TTGCCCATAC TATATGTTTT AATTTATATT TTTTCTTTCC CCCTGGCCTT AACCGAATTT TTTCCCATCG CGATCTAATT CTCCCCCGCT TAATACTGAC GCTCTCGCAC CCATCTCTCT CCTTCTAGCC TCCGCTAGTC AAAATTTTTG GCGTACTCAC CAGTCGCCGC CCCTCGCCTC TTGCCGTGCG CGCTTCAGCA AGCCGAGTCC TGCGTCGAGA GAGCTCCTCT GGTTTCCCTT TCGCTTTCAA GTCCCTGTTC GGGCGCCACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGGCA AAAAGCAGCT GCTTATATGC AGGATCTGAG GGCTCGCCAC TCCCCAGTCC CGCCCAGGCC ACGCCTCCCT GGAAAGTCCC CAGCGGAAAG TCCCTTGTAG CAAGCTCGAT ATCAGCAGTT CTTGAAGTAC TCCGGATGCA GCTCTCGGGC CACGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AACACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCTGGTG TTCTCTCCTT TATTGGCCTC TTCTACCTTA TCTGGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGACCCCT GGCCCTGGTG TGTAGTTCTG CTAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT CTTGTCTTCT TTGGGAGTGA ATTAGCCCTT CCA
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