pAAV-CamKII-Pdk2(mouse)-2×HA
$70.00
In stock
SKU
PL40516
NM_133667.2
100μg liquid plasmid(Instantly):$91
| name | pAAV-CamKII-Pdk2(mouse)-2×HA | another name | apAAV-CamKII-Pdk2(mouse)-2×HA |
| Insert Size (bp) | 1224bp | Plasmid host | Mammary cell,Adeno-associated virus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CaMKIIa | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTTTAAC ATTATGGCCT TAGGTCACTT CATCTCCATG GGGTTCTTCT TCTGATTTTC TAGAAAATGA GATGGGGGTG CAGAGAGCTT CCTCAGTGAC CTGCCCAGGG TCACATCAGA AATGTCAGAG CTAGAACTTG AACTCAGATT ACTAATCTTA AATTCCATGC CTTGGGGGCA TGCAAGTACG ATATACAGAA GGAGTGAACT CATTAGGGCA GATGACCAAT GAGTTTAGGA AAGAAGAGTC CAGGGCAGGG TACATCTACA CCACCCGCCC AGCCCTGGGT GAGTCCAGCC ACGTTCACCT CATTATAGTT GCCTCTCTCC AGTCCTACCT TGACGGGAAG CACAAGCAGA AACTGGGACA GGAGCCCCAG GAGACCAAAT CTTCATGGTC CCTCTGGGAG GATGGGTGGG GAGAGCTGTG GCAGAGGCCT CAGGAGGGGC CCTGCTGCTC AGTGGTGACA GATAGGGGTG AGAAAGCAGA CAGAGTCATT CCGTCAGCAT TCTGGGTCTG TTTGGTACTT CTTCTCACGC TAAGGTGGCG GTGTGATATG CACAATGGCT AAAAAGCAGG GAGAGCTGGA AAGAAACAAG GACAGAGACA GAGGCCAAGT CAACCAGACC AATTCCCAGA GGAAGCAAAG AAACCATTAC AGAGACTACA AGGGGGAAGG GAAGGAGAGA TGAATTAGCT TCCCCTGTAA ACCTTAGAAC CCAGCTGTTG CCAGGGCAAC GGGGCAATAC CTGTCTCTTC AGAGGAGATG AAGTTGCCAG GGTAACTACA TCCTGTCTTT CTCAAGGACC ATCCCAGAAT GTGGCACCCA CTAGCCGTTA CCATAGCAAC TGCCTCTTTG CCCCACTTAA TCCCATCCCG TCTGTTAAAA GGGCCCTATA GTTGGAGGTG GGGGAGGTAG GAAGAGCGAT GATCACTTGT GGACTAAGTT TGTTCGCATC CCCTTCTCCA ACCCCCTCAG TACATCACCC TGGGGGAACA GGGTCCACTT GCTCCTGGGC CCACACAGTC CTGCAGTATT GTGTATATAA GGCCAGGGCA AAGAGGAGCA GGTTTTAAAG TGAAAGGCAG GCAGGTGTTG GGGAGGCAGT TACCGGGGCA ACGGGAACAG GGCGTTTCGG AGGTGGTTGC CATGGGGACC TGGATGCTGA CGAAGGCTCG CGAGGCTGTG AGCAGCCACA GTGCCCTGCT CAGAAGCCCC AAGCTCGTCA GTCAAGCCGG TTCTCCGTTT GCACTCAGGA GCACGGGCAG GCGAGTGGCC CCTAGTTCTG GGGGCAGCTC TAGAGCGGTA CCGGATCCAC TAGTGCCACC ATGCGCTGGG TCCGGGCGCT GTTGAAGAAT GCGTCCCTGG CAGGGGCACC CAAGTACATC GAGCACTTCA GCAAGTTCTC CCCGTCCCCG TTGTCCATGA AGCAGTTTCT AGACTTCGGA TCCAGCAATG CCTGCGAGAA GACGTCATTC ACTTTCCTCC GGCAGGAGCT GCCCGTTCGC CTGGCCAACA TCATGAAAGA GATCAACCTG CTTCCTGACC GAGTGCTGGG CACCCCATCG GTGCAGCTGG TGCAGAGCTG GTATGTCCAG AGTCTGCTGG ACATCATGGA ATTCCTGGAC AAAGACCCCG AGGACCACCG GACTCTAAGC CAGTTCACAG ATGCCCTGGT CACCATCCGG AACCGGCACA ATGACGTGGT GCCCACCATG GCACAGGGAG TGCTGGAGTA CAAGGACACC TATGGAGATG ACCCCGTCTC CAACCAGAAC ATCCAGTACT TCCTGGACCG CTTCTACCTC AGCCGCATCT CCATCCGAAT GCTAATCAAT CAACACACCC TCATCTTTGA TGGCAGCACC AACCCAGCCC ACCCCAAACA CATTGGCAGC ATCGATCCCA ACTGCAGCGT GTCTGATGTG GTGAAAGACG CCTATGACAT GGCTAAGCTC CTGTGTGACA AGTATTACAT GGCTTCCCCT GACCTGGAGA TCCAGGAAGT CAATGCCACC AACGCCAACC AGCCCATTCA CATGGTCTAT GTCCCTTCCC ACCTGTACCA CATGCTCTTT GAACTCTTTA AGAATGCCAT GCGGGCCACT GTGGAAAGCC ACGAGTCCAG CCTCACACTC CCCCCCATCA AGATTATGGT GGCCCTCGGT GAAGAAGATC TGTCCATCAA AATGAGTGAC CGAGGCGGGG GTGTCCCCTT GAGGAAGATC GAGAGGCTCT TCAGCTACAT GTACTCCACA GCTCCCACAC CCCAGCCTGG CACTGGGGGT ACCCCGCTGG CTGGCTTTGG GTACGGACTC CCCATTTCCC GCCTCTACGC CAAGTACTTC CAGGGGGACT TGCAGCTTTT CTCTATGGAG GGCTTTGGGA CAGATGCTGT CATCTATCTG AAGGCCCTGT CCACGGACTC AGTGGAGCGC CTGCCTGTCT ACAACAAGTC TGCCTGGCGC CACTACCAGA CCATCCAGGA GGCCGGCGAC TGGTGTGTGC CCAGCACAGA GCCCAAGAAC ACATCGACGT ATCGGGTCAG CGCTAGCTAC CCATACGACG TACCTGATTA TGCATCATAC CCATACGATG TTCCAGATTA CGCTTAGTGA ATTCACCGGT TAGTAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT ATGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCATCGA TACCGAGCGC TGCTCGAGAG ATCTACGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TGTAGGTAAC CACGTGCGGA CCGAGCGGCC GCAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA CCAAAGGTCG CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC AGCTGCCTGC AGGGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAC GTCAAAGCAA CCATAGTACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTTGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGG CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGTTTACAA TTTTATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT
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