pCMV-UBE2O(human)-Neo
$99.00
In stock
SKU
PL40481
NM_022066.3;E2-230K
100μg liquid plasmid(Instantly):$120
| name | pCMV-UBE2O(human)-Neo | another name | apCMV-UBE2O(human)-Neo |
| Insert Size (bp) | 3879bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCCACCAT GGCGGATCCC GCAGCCCCCA CGCCCGCAGC TCCCGCTCCA GCCCAGGCCC CGGCTCCAGC CCCGGAGGCA GTCCCGGCCC CAGCCGCAGC CCCCGTCCCG GCGCCGGCGC CCGCCTCGGA CTCGGCCTCC GGGCCGTCCT CGGACTCCGG CCCAGAAGCC GGCTCGCAGC GCCTGCTGTT TTCTCACGAC CTGGTGTCGG GCCGTTACCG TGGCTCCGTG CACTTCGGGC TGGTGCGCCT CATCCACGGC GAGGACTCGG ACTCGGAGGG CGAGGAGGAG GGCCGCGGGA GCTCGGGGTG CTCCGAGGCC GGGGGCGCGG GCCACGAGGA GGGCCGGGCC AGCCCCCTGC GCCGCGGCTA CGTGCGCGTC CAGTGGTACC CGGAGGGCGT CAAGCAGCAT GTGAAGGAGA CCAAGCTGAA ACTAGAGGAC CGTTCTGTGG TGCCCCGAGA TGTGGTCCGG CACATGCGAT CCACCGACAG TCAGTGTGGC ACGGTGATCG ACGTCAACAT CGACTGTGCC GTCAAGCTCA TCGGCACCAA CTGCATCATC TATCCCGTCA ACAGCAAGGA CCTGCAGCAC ATCTGGCCCT TCATGTATGG GGACTACATT GCCTATGACT GCTGGCTGGG GAAGGTCTAC GACTTGAAGA ACCAGATCAT CCTGAAGCTA TCCAACGGCG CCAGGTGCTC CATGAACACG GAAGATGGCG CCAAGCTCTA CGACGTCTGC CCGCACGTCA GCGACTCGGG TCTCTTCTTC GATGATTCCT ATGGCTTCTA CCCAGGCCAG GTGCTCATTG GCCCTGCCAA GATCTTCTCC AGCGTCCAGT GGCTGTCAGG TGTCAAGCCC GTGCTCAGCA CCAAGAGCAA GTTCCGAGTG GTGGTGGAAG AGGTGCAGGT TGTAGAGTTG AAAGTTACAT GGATTACCAA GAGTTTCTGT CCAGGGGGCA CGGACAGCGT CAGCCCCCCA CCCTCTGTCA TCACCCAGGA AAACCTAGGC AGGGTGAAGC GTCTCGGATG CTTTGACCAT GCTCAGCGGC AGCTTGGGGA GCGCTGTCTG TATGTCTTCC CAGCCAAGGT AGAGCCAGCC AAGATTGCCT GGGAATGTCC AGAAAAAAAC TGCGCCCAGG GGGAGGGCTC TATGGCCAAG AAGGTGAAGC GCCTGTTGAA GAAGCAGGTT GTGCGGATCA TGTCATGCTC CCCAGACACC CAGTGTTCCC GGGACCATTC CATGGAAGAC CCAGACAAGA AGGGGGAATC CAAAACCAAG AGCGAAGCGG AGTCTGCCAG CCCTGAGGAG ACGCCCGATG GCTCTGCCAG TCCAGTGGAG ATGCAGGACG AGGGTGCAGA GGAGCCCCAC GAGGCAGGAG AGCAGCTGCC CCCATTCCTG CTAAAAGAAG GCAGAGATGA CAGGCTGCAC TCGGCAGAGC AGGACGCAGA TGATGAGGCT GCTGATGACA CGGACGACAC CAGTTCGGTG ACCTCCTCTG CCAGCTCCAC CACTTCCTCC CAGAGCGGCA GCGGCACGAG TCGCAAAAAG AGCATCCCCT TGTCCATCAA GAACTTAAAG CGCAAACACA AGAGGAAGAA GAATAAAATC ACTCGAGACT TCAAGCCAGG GGACAGGGTG GCAGTGGAGG TGGTGACCAC GATGACCTCA GCCGACGTGA TGTGGCAGGA TGGCTCCGTG GAATGCAACA TCCGCTCCAA CGACCTCTTC CCTGTGCACC ACCTGGACAA CAACGAGTTC TGCCCTGGAG ACTTCGTGGT AGATAAGCGA GTCCAGAGCT GTCCAGACCC TGCTGTCTAC GGTGTGGTAC AGTCTGGGGA CCACATCGGC CGTACCTGCA TGGTGAAGTG GTTCAAGCTG AGGCCGAGTG GGGACGACGT GGAGCTGATT GGAGAAGAGG AAGATGTGAG TGTTTACGAC ATTGCTGACC ACCCTGACTT TAGGTTCCGT ACAACTGACA TCGTCATCCG CATCGGCAAT ACTGAGGATG GGGCTCCTCA CAAGGAGGAT GAGCCATCGG TGGGCCAGGT GGCCCGTGTG GACGTCAGCA GCAAGGTGGA GGTGGTGTGG GCTGACAACT CAAAGACCAT CATCCTGCCC CAGCACTTGT ACAACATAGA GTCTGAGATT GAGGAGTCAG ACTACGATTC GGTAGAAGGC AGCACCAGCG GGGCATCCTC GGATGAATGG GAAGATGATA GTGACAGCTG GGAGACGGAC AATGGGCTGG TGGAGGACGA GCACCCCAAG ATAGAGGAGC CCCCCATCCC ACCCCTGGAG CAGCCGGTGG CCCCTGAGGA CAAGGGAGTG GTGATCAGTG AAGAGGCAGC CACAGCTGCC GTCCAGGGGG CTGTGGCCAT GGCTGCCCCC ATGGCCGGGC TGATGGAGAA GGCTGGCAAG GACGGGCCAC CCAAGAGCTT CCGGGAGTTG AAAGAGGCCA TCAAGATCCT GGAGAGCCTC AAGAACATGA CTGTGGAGCA GCTGCTGACG GGCTCGCCCA CCTCTCCGAC TGTGGAGCCT GAGAAGCCAA CTCGGGAGAA GAAGTTTCTG GATGACATCA AGAAGCTACA GGAAAACCTC AAGAAGACCC TGGACAATGT GGCCATTGTA GAGGAGGAGA AGATGGAAGC AGTGCCCGAC GTAGAGCGCA AGGAGGACAA GCCCGAGGGG CAGTCACCTG TGAAGGCTGA GTGGCCCAGC GAAACCCCGG TGCTGTGCCA GCAGTGTGGC GGCAAGCCTG GCGTCACCTT CACCAGCGCC AAGGGCGAGG TCTTCTCCGT ACTGGAGTTT GCACCCTCAA ATCATTCTTT TAAGAAAATT GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC TTCAGCACAG TGCGGAAGGA GATGGCGCTG CTGGCTACCT CACTGCCTGA GGGCATCATG GTCAAGACTT TTGAAGATAG AATGGACCTC TTCTCAGCTC TCATCAAGGG CCCCACTCGA ACCCCCTACG AGGATGGCCT CTACTTGTTT GACATCCAGC TCCCCAACAT CTACCCAGCC GTGCCCCCCC ACTTCTGCTA CCTCTCCCAA TGCAGTGGCC GCCTGAACCC CAACCTGTAT GACAATGGGA AGGTGTGTGT CAGCCTCCTG GGCACCTGGA TTGGAAAGGG GACAGAGAGG TGGACAAGCA AGTCCAGCCT TCTCCAGGTG CTCATCTCCA TCCAAGGTCT GATCCTGGTA AATGAACCAT ACTACAACGA AGCCGGCTTC GACAGTGACC GAGGCCTGCA GGAAGGCTAT GAAAACAGTC GCTGTTACAA TGAGATGGCG CTGATCCGCG TGGTGCAGTC CATGACCCAG CTGGTGCGGC GGCCCCCCGA GGTCTTTGAG CAGGAGATCA GGCAACACTT TAGCACTGGT GGCTGGCGGC TGGTGAACCG TATCGAGTCC TGGCTGGAAA CCCATGCCCT GCTGGAGAAG GCCCAGGCAC TGCCCAACGG GGTGCCCAAG GCCAGCAGCT CGCCAGAGCC CCCAGCTGTA GCCGAGCTGT CAGACTCCGG CCAACAAGAA CCTGAGGATG GAGGGCCAGC CCCAGGAGAG GCCTCCCAGG GCTCAGACTC AGAGGGCGGT GCCCAGGGCC TGGCCTCAGC TAGCAGGGAC CACACAGACC AGACTTCGGA GACCGCACCA GACGCATCGG TGCCACCCAG TGTGAAACCA AAGAAGCGGA GAAAGAGCTA CCGGAGCTTC TTACCTGAGA AGAGTGGCTA CCCTGACATC GGCTTCCCCC TCTTCCCACT TTCCAAGGGT TTCATCAAGA GCATCCGGGG TGTCCTGACG CAGTTCCGGG CTGCCCTGCT AGAGGCAGGC ATGCCGGAGT GCACAGAGGA CAAGTAGGAT ATCCAGCACA GTGGCGGCCG CTCGAGTCTA GAGGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTC
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