pSV40-hRluc-5UTR-JAK3(human)-3UTR-Fluc
$84.00
In stock
SKU
PL40466
100μg liquid plasmid(Instantly):$105
| name | pSV40-hRluc-5UTR-JAK3(human)-3UTR-Fluc | another name | apSV40-hRluc-5UTR-JAK3(human)-3UTR-Fluc |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | UTR |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Rluc,Fluc | |
| Promoter | SV40 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC ACTTTGCCTT TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA GTACTTAATA CGACTCACTA TAGGCTAGCC ACCATGGCTT CCAAGGTGTA CGACCCCGAG CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT CTGCATGGTA ACGCTGCCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGG CTTGTCTGGC CTTTCACTAC TCCTACGAGC ACCAAGACAA GATCAAGGCC ATCGTCCATG CTGAGAGTGT CGTGGACGTG ATCGAGTCCT GGGACGAGTG GCCTGACATC GAGGAGGATA TCGCCCTGAT CAAGAGCGAA GAGGGCGAGA AAATGGTGCT TGAGAATAAC TTCTTCGTCG AGACCATGCT CCCAAGCAAG ATCATGCGGA AACTGGAGCC TGAGGAGTTC GCTGCCTACC TGGAGCCATT CAAGGAGAAG GGCGAGGTTA GACGGCCTAC CCTCTCCTGG CCTCGCGAGA TCCCTCTCGT TAAGGGAGGC AAGCCCGACG TCGTCCAGAT TGTCCGCAAC TACAACGCCT ACCTTCGGGC CAGCGACGAT CTGCCTAAGA TGTTCATCGA GTCCGACCCT GGGTTCTTTT CCAACGCTAT TGTCGAGGGA GCTAAGAAGT TCCCTAACAC CGAGTTCGTG AAGGTGAAGG GCCTCCACTT CAGCCAGGAG GACGCTCCAG ATGAAATGGG TAAGTACATC AAGAGCTTCG TGGAGCGCGT GCTGAAGAAC GAGCAGTAAT TCTAGGCGAT CGCTCGAGGC CCGCCCTTCG AAAGTCCAGG GTCCCTGCCC GCTAGGCAAG TTGCACTCAT GGCACCTCCA AGTGAAGAGA CGCCCCTGAT CCCTCAGCGT TCATGCAGCC TCTTGTCCAC GGAGGCTGGT GCCCTGCATG TGCTGCTGCC CGCTCGGGGC CCCGGGCCCC CCCAGCGCCT ATCTTTCTCC TTTGGGGACC ACTTGGCTGA GGACCTGTGC GTGCAGGCTG CCAAGGCCAG CGGCATCCTG CCTGTGTACC ACTCCCTCTT TGCTCTGGCC ACGGAGGACC TGTCCTGCTG GTTCCCCCCG AGCCACATCT TCTCCGTGGA GGATGCCAGC ACCCAAGTCC TGCTGTACAG GATTCGCTTT TACTTCCCCA ATTGGTTTGG GCTGGAGAAG TGCCACCGCT TCGGGCTACG CAAGGATTTG GCCAGTGCTA TCCTTGACCT GCCAGTCCTG GAGCACCTCT TTGCCCAGCA CCGCAGTGAC CTGGTGAGTG GGCGCCTCCC CGTGGGCCTC AGTCTCAAGG AGCAGGGTGA GTGTCTCAGC CTGGCCGTGT TGGACCTGGC CCGGATGGCG CGAGAGCAGG CCCAGCGGCC GGGAGAGCTG CTGAAGACTG TCAGCTACAA GGCCTGCCTA CCCCCAAGCC TGCGCGACCT GATCCAGGGC CTGAGCTTCG TGACGCGGAG GCGTATTCGG AGGACGGTGC GCAGAGCCCT GCGCCGCGTG GCCGCCTGCC AGGCAGACCG GCACTCGCTC ATGGCCAAGT ACATCATGGA CCTGGAGCGG CTGGATCCAG CCGGGGCCGC CGAGACCTTC CACGTGGGCC TCCCTGGGGC CCTTGGTGGC CACGACGGGC TGGGGCTGCT CCGCGTGGCT GGTGACGGCG GCATCGCCTG GACCCAGGGA GAACAGGAGG TCCTCCAGCC CTTCTGCGAC TTTCCAGAAA TCGTAGACAT TAGCATCAAG CAGGCCCCGC GCGTTGGCCC GGCCGGAGAG CACCGCCTGG TCACTGTTAC CAGGACAGAC AACCAGATTT TAGAGGCCGA GTTCCCAGGG CTGCCCGAGG CTCTGTCGTT CGTGGCGCTC GTGGACGGCT ACTTCCGGCT GACCACGGAC TCCCAGCACT TCTTCTGCAA GGAGGTGGCA CCGCCGAGGC TGCTGGAGGA AGTGGCCGAG CAGTGCCACG GCCCCATCAC TCTGGACTTT GCCATCAACA AGCTCAAGAC TGGGGGCTCA CGTCCTGGCT CCTATGTTCT CCGCCGCAGC CCCCAGGACT TTGACAGCTT CCTCCTCACT GTCTGTGTCC AGAACCCCCT TGGTCCTGAT TATAAGGGCT GCCTCATCCG GCGCAGCCCC ACAGGAACCT TCCTTCTGGT TGGCCTCAGC CGACCCCACA GCAGTCTTCG AGAGCTCCTG GCAACCTGCT GGGATGGGGG GCTGCACGTA GATGGGGTGG CAGTGACCCT CACTTCCTGC TGTATCCCCA GACCCAAAGA AAAGTCCAAC CTGATCGTGG TCCAGAGAGG TCACAGCCCA CCCACATCAT CCTTGGTTCA GCCCCAATCC CAATACCAGC TGAGTCAGAT GACATTTCAC AAGATCCCTG CTGACAGCCT GGAGTGGCAT GAGAACCTGG GCCATGGGTC CTTCACCAAG ATTTACCGGG GCTGTCGCCA TGAGGTGGTG GATGGGGAGG CCCGAAAGAC AGAGGTGCTG CTGAAGGTCA TGGATGCCAA GCACAAGAAC TGCATGGAGT CATTCCTGGA AGCAGCGAGC TTGATGAGCC AAGTGTCGTA CCGGCATCTC GTGCTGCTCC ACGGCGTGTG CATGGCTGGA GACAGCACCA TGGTGCAGGA ATTTGTACAC CTGGGGGCCA TAGACATGTA TCTGCGAAAA CGTGGCCACC TGGTGCCAGC CAGCTGGAAG CTGCAGGTGG TCAAACAGCT GGCCTACGCC CTCAACTATC TGGAGGACAA AGGCCTGCCC CATGGCAATG TCTCTGCCCG GAAGGTGCTC CTGGCTCGGG AGGGGGCTGA TGGGAGCCCG CCCTTCATCA AGCTGAGTGA CCCTGGGGTC AGCCCCGCTG TGTTAAGCCT GGAGATGCTC ACCGACAGGA TCCCCTGGGT GGCCCCCGAG TGTCTCCGGG AGGCGCAGAC ACTTAGCTTG GAAGCTGACA AGTGGGGCTT CGGCGCCACG GTCTGGGAAG TGTTTAGTGG CGTCACCATG CCCATCAGTG CCCTGGATCC TGCTAAGAAA CTCCAATTTT ATGAGGACCG GCAGCAGCTG CCGGCCCCCA AGTGGACAGA GCTGGCCCTG CTGATTCAAC AGTGCATGGC CTATGAGCCG GTCCAGAGGC CCTCCTTCCG AGCCGTCATT CGTGACCTCA ATAGCCTCAT CTCTTCAGAC TATGAGCTCC TCTCAGACCC CACACCTGGT GCCCTGGCAC CTCGTGATGG GCTGTGGAAT GGTGCCCAGC TCTATGCCTG CCAAGACCCC ACGATCTTCG AGGAGAGACA CCTCAAGTAC ATCTCACAGC TGGGCAAGGG CAACTTTGGC AGCGTGGAGC TGTGCCGCTA TGACCCGCTA GGCGACAATA CAGGTGCCCT GGTGGCCGTG AAACAGCTGC AGCACAGCGG GCCAGACCAG CAGAGGGACT TTCAGCGGGA GATTCAGATC CTCAAAGCAC TGCACAGTGA TTTCATTGTC AAGTATCGTG GTGTCAGCTA TGGCCCGGGC CGCCAGAGCC TGCGGCTGGT CATGGAGTAC CTGCCCAGCG GCTGCTTGCG CGACTTCCTG CAGCGGCACC GCGCGCGCCT CGATGCCAGC CGCCTCCTTC TCTATTCCTC GCAGATCTGC AAGGGCATGG AGTACCTGGG CTCCCGCCGC TGCGTGCACC GCGACCTGGC CGCCCGAAAC ATCCTCGTGG AGAGCGAGGC ACACGTCAAG ATCGCTGACT TCGGCCTAGC TAAGCTGCTG CCGCTTGACA AAGACTACTA CGTGGTCCGC GAGCCAGGCC AGAGCCCCAT TTTCTGGTAT GCCCCCGAAT CCCTCTCGGA CAACATCTTC TCTCGCCAGT CAGACGTCTG GAGCTTCGGG GTCGTCCTGT ACGAGCTCTT CACCTACTGC GACAAAAGCT GCAGCCCCTC GGCCGAGTTC CTGCGGATGA TGGGATGTGA GCGGGATGTC CCCGCCCTCT GCCGCCTCTT GGAACTGCTG GAGGAGGGCC AGAGGCTGCC GGCGCCTCCT GCCTGCCCTG CTGAGGTTCA CGAGCTCATG AAGCTGTGCT GGGCCCCTAG CCCACAGGAC CGGCCATCAT TCAGCGCCCT GGGCCCCCAG CTGGACATGC TGTGGAGCGG AAGCCGGGGG TGTGAGACTC ATGCCTTCAC TGCTCACCCA GAGGGCAAAC ACCACTCCCT GTCCTTTTCA TAGCTCCTGC CCGCAGACCT CTGGATTAGG TCTCTGTTGA CTGGCTGTGT GACCTTAGGC CCGGAGCTGC CCCTCTCTGG GCCTCAGAGG CCTTATGAGG GTCCTCTACT TCAGGAACAC CCCCATGACA TTGCATTTGG GGGGGCTCCC GTGGCCTGTA GAATAGCCTG TGGCCTTTGC AATTTGTTAA GGTTCAAGAC AGATGGGCAT ATGTGTCAGT GGGGCTCTCT GAGTCCTGGC CCAAAGAAGC AAGGAACCAA ATTTAAGACT CTCGCATCTT CCCAACCCCT TAAGCCCTGG CCCCCTGAGT TTCCTTTTCT GTCTCTCTCT TTTTATTTTT TTTATTTTTA TTTTTATTTT TGAGACAGAG CCTCGCTCTG TTACCCAGGG TGGAGTGCAG TGGTGCGATC TCGGCTCAGT GCAACCTCTG CTTCCCAGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTAGCTGGGA TTACAGGTGT GCACCACCAC ACCCGGCTAA TTTTTTTTAT TTTTAATAGA GATGAGGTTT CACCATGATG GCCAGGCTGA TCTCGAACTC CTAACCTCAA GTGATCCTCC CACCTCAGCC TCCCAAAGTG TTGGAATAAT AGGCATGAGC CACTGCACCC AGGCTTTTTT TTTTTTAAAT TTATTATTAT TATTTTTAAG AGACAGGATC TTGCTACGTT GCCCAGGCTG GTCTTGAACT CCTGGGCTAC AGTGATCCTC CTGCCTTATC CTCCTAAATA GCTGGGACTA CAGCACCTAG TTTTGAGTTT CCTGTCTTAT TTCCAATGGG GACATTCATG TAGCTTTTTT TTTTTTTTTT TTTTTGAGAC GGAGTCTCGC TCTGTCGCCC AGGCTGGAGT ACAGTGGCGC AATCTAGGCT CACTGCAAGC TCCGCCTCCT GGGTTCACAC CATTCTCTCG CCTCAGCCTC CCAAGTAGCT GGGACTACAG GCGCCCGCCA CCACACCCGG CTAATTTTTT GTATTTTTAG TAGAGACGGG GTTTCACCTT GTTAGCCAGG ATGGTTTCCA TCTCCTGACC TCGTGATCTG CCCGTCTCGG CCTCCCAAAG TGCTGGGATT ACAGGCATGA GCCACTGCGC CCGGCCCTCA TGTAGCTTTA AATGTATGAT CTGACTTCTG CTCCCCGATC TCTGTTTCTC TGGAGGAAGC CAAGGACAAG AGCAGTTGCT GTGGCTGGGA CTCTGCCTTT TAGGGGAGCC CGTGTATCTC TTTGGGATCC TGAAAGGGGG CAGGAAAGGC TGGGGTCCCA GTCCACCCTA ATGGTATCTG AGTGTCCTAG GGCTTCAGTT TTCCCACCTG TCCAATGGGA CCCTTTCTGT CCTCACCCTA CAAGGGGCAC AAAGGGATGA CACCAAACCT GGCAGGAACT TTTCACGCAA TCAAGGGAAG GAAAGGCATT CCTGGCAGAG GGAACAGCAT GCCAAGCGTG AGAAGGCTCA GAGTAAGGAG GTTAAGAGCC CAAGTATTGG AGCCTACAGT TTTGCCCCTT CCATGCAGTG TGACAGTGGG CAAGTTCCTT TCCCTCTCTG GGTCTCAGTT CTGTCCCCTG CAAAATGGTC AGAGCTTACC CCTTGGCTGT GCAGGGTCAA CTTTCTGACT GGTGAGAGGG ATTCTCATGC AGGTTAAGCT TCTGCTGCTC CTCCTCACCT GCAAAGCTTT TCTGCCACTT TTGCCTCCTT GGAAAACTCT TATCCATCTC TCAAAACTCC AGCTACCACA TCCTTGCAGC CTTCCCTCAT ATACCCCCAC TACTACTGTA GCCCTGTCCT TCCCTCCAGC CCCACTCTGG CCCTGGGGCT GGGGAAGTGT CTGTGTCCAG CTGTCTCCCC TGACCTCAGG GTTCCTTGGG GGCTGGGCTG AGGCCTCAGT ACAGAGGGGG CTCTGGAAAT GTTTGTTGAC TGAATAAAGG AATTCAGTGG AAAACCCGGG AATTCGTTTA AACCTAGAGC GGCCGCTGGC CGCAATAAAA TATCTTTATT TTCATTACAT CTGTGTGTTG GTTTTTTGTG TGAGGATCTA AATGAGTCTT CGGACCTCGC GGGGGCCGCT TAAGCGGTGG TTAGGGTTTG TCTGACGCGG GGGGAGGGGG AAGGAACGAA ACACTCTCAT TCGGAGGCGG CTCGGGGTTT GGTCTTGGTG GCCACGGGCA CGCAGAAGAG CGCCGCGATC CTCTTAAGCA CCCCCCCGCC CTCCGTGGAG GCGGGGGTTT GGTCGGCGGG TGGTAACTGG CGGGCCGCTG ACTCGGGCGG GTCGCGCGCC CCAGAGTGTG ACCTTTTCGG TCTGCTCGCA GACCCCCGGG CGGCGCCGCC GCGGCGGCGA CGGGCTCGCT GGGTCCTAGG CTCCATGGGG ACCGTATACG TGGACAGGCT CTGGAGCATC CGCACGACTG CGGTGATATT ACCGGAGACC TTCTGCGGGA CGAGCCGGGT CACGCGGCTG ACGCGGAGCG TCCGTTGGGC GACAAACACC AGGACGGGGC ACAGGTACAC TATCTTGTCA CCCGGAGGCG CGAGGGACTG CAGGAGCTTC AGGGAGTGGC GCAGCTGCTT CATCCCCGTG GCCCGTTGCT CGCGTTTGCT GGCGGTGTCC CCGGAAGAAA TATATTTGCA TGTCTTTAGT TCTATGATGA CACAAACCCC GCCCAGCGTC TTGTCATTGG CGAATTCGAA CACGCAGATG CAGTCGGGGC GGCGCGGTCC CAGGTCCACT TCGCATATTA AGGTGACGCG TGTGGCCTCG AACACCGAGC GACCCTGCAG CGACCCGCTT AAAAGCTTGG CATTCCGGTA CTGTTGGTAA AGCCACCATG GCCGATGCTA AGAACATTAA GAAGGGCCCT GCTCCCTTCT ACCCTCTGGA GGATGGCACC GCTGGCGAGC AGCTGCACAA GGCCATGAAG AGGTATGCCC TGGTGCCTGG CACCATTGCC TTCACCGATG CCCACATTGA GGTGGACATC ACCTATGCCG AGTACTTCGA GATGTCTGTG CGCCTGGCCG AGGCCATGAA GAGGTACGGC CTGAACACCA ACCACCGCAT CGTGGTGTGC TCTGAGAACT CTCTGCAGTT CTTCATGCCA GTGCTGGGCG CCCTGTTCAT CGGAGTGGCC GTGGCCCCTG CTAACGACAT TTACAACGAG CGCGAGCTGC TGAACAGCAT GGGCATTTCT CAGCCTACCG TGGTGTTCGT GTCTAAGAAG GGCCTGCAGA AGATCCTGAA CGTGCAGAAG AAGCTGCCTA TCATCCAGAA GATCATCATC ATGGACTCTA AGACCGACTA CCAGGGCTTC CAGAGCATGT ACACATTCGT GACATCTCAT CTGCCTCCTG GCTTCAACGA GTACGACTTC GTGCCAGAGT CTTTCGACAG GGACAAAACC ATTGCCCTGA TCATGAACAG CTCTGGGTCT ACCGGCCTGC CTAAGGGCGT GGCCCTGCCT CATCGCACCG CCTGTGTGCG CTTCTCTCAC GCCCGCGACC CTATTTTCGG CAACCAGATC ATCCCCGACA CCGCTATTCT GAGCGTGGTG CCATTCCACC ACGGCTTCGG CATGTTCACC ACCCTGGGCT ACCTGATTTG CGGCTTTCGG GTGGTGCTGA TGTACCGCTT CGAGGAGGAG CTGTTCCTGC GCAGCCTGCA AGACTACAAA ATTCAGTCTG CCCTGCTGGT GCCAACCCTG TTCAGCTTCT TCGCTAAGAG CACCCTGATC GACAAGTACG ACCTGTCTAA CCTGCACGAG ATTGCCTCTG GCGGCGCCCC ACTGTCTAAG GAGGTGGGCG AAGCCGTGGC CAAGCGCTTT CATCTGCCAG GCATCCGCCA GGGCTACGGC CTGACCGAGA CAACCAGCGC CATTCTGATT ACCCCAGAGG GCGACGACAA GCCTGGCGCC GTGGGCAAGG TGGTGCCATT CTTCGAGGCC AAGGTGGTGG ACCTGGACAC CGGCAAGACC CTGGGAGTGA ACCAGCGCGG CGAGCTGTGT GTGCGCGGCC CTATGATTAT GTCCGGCTAC GTGAATAACC CTGAGGCCAC AAACGCCCTG ATCGACAAGG ACGGCTGGCT GCACTCTGGC GACATTGCCT ACTGGGACGA GGACGAGCAC TTCTTCATCG TGGACCGCCT GAAGTCTCTG ATCAAGTACA AGGGCTACCA GGTGGCCCCA GCCGAGCTGG AGTCTATCCT GCTGCAGCAC CCTAACATTT TCGACGCCGG AGTGGCCGGC CTGCCCGACG ACGATGCCGG CGAGCTGCCT GCCGCCGTCG TCGTGCTGGA ACACGGCAAG ACCATGACCG AGAAGGAGAT CGTGGACTAT GTGGCCAGCC AGGTGACAAC CGCCAAGAAG CTGCGCGGCG GAGTGGTGTT CGTGGACGAG GTGCCCAAGG GCCTGACCGG CAAGCTGGAC GCCCGCAAGA TCCGCGAGAT CCTGATCAAG GCTAAGAAAG GCGGCAAGAT CGCCGTGTAA TAATTCTAGA GTCGGGGCGG CCGGCCGCTT CGAGCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTAAAAT CGATAAGGAT CCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGGCTCGAC
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