pCMV-mCherry-SUR1(human)-6×His-Neo
$113.00
In stock
SKU
PL40338
100μg liquid plasmid(Instantly):$134
| name | pCMV-mCherry-SUR1(human)-6×His-Neo | another name | apCMV-mCherry-SUR1(human)-6×His-Neo |
| Insert Size (bp) | 4743bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGGATAACA TGGCCATCAT CAAGGAGTTC ATGCGCTTCA AGGTGCACAT GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC GAGGGCGAGG GCCGCCCCTA CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG GGACATCCTG TCCCCTCAGT TCATGTACGG CTCCAAGGCC TACGTGAAGC ACCCCGCCGA CATCCCCGAC TACTTGAAGC TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC GCGTGATGAA CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC TCCCTGCAGG ACGGCGAGTT CATCTACAAG GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG TAATGCAGAA GAAGACCATG GGCTGGGAGG CCTCCTCCGA GCGGATGTAC CCCGAGGACG GCGCCCTGAA GGGCGAGATC AAGCAGAGGC TGAAGCTGAA GGACGGCGGC CACTACGACG CTGAGGTCAA GACCACCTAC AAGGCCAAGA AGCCCGTGCA GCTGCCCGGC GCCTACAACG TCAACATCAA GTTGGACATC ACCTCCCACA ACGAGGACTA CACCATCGTG GAACAGTACG AACGCGCCGA GGGCCGCCAC TCCACCGGCG GCATGGACGA GCTGTACAAG TCCGGACTCA GATCTCGAGC TGGAGGTGGA GGTAGTGGAG GAGGCGGAAG CAAGCTTATG CCCCTGGCCT TCTGCGGCAG CGAGAACCAC TCGGCCGCCT ACCGGGTGGA CCAGGGGGTC CTCAACAACG GCTGCTTTGT GGACGTCCTC AACGTGGTGC CGCACGTCTT CCTACTCTTC ATCACCTTCC CCATCCTCTT CATTGGATGG GGAAGTCAGA GCTCCAAGGT GCACATCCAC CACAGCACAT GGCTTCATTT CCCTGGGCAC AACCTGCGGT GGATCCTGAC CTTCATGCTG CTCTTCGTCC TGGTGTGTGA GATTGCAGAG GGCATCCTGT CTGATGGGGT GACCGAATCC CACCATCTGC ACCTGTACAT GCCAGCCGGG ATGGCGTTCA TGGCTGCTGT CACCTCCGTG GTCTACTATC ACAACATCGA GACTTCCAAC TTCCCCAAGC TGCTAATTGC CCTGCTGGTG TATTGGACCC TGGCCTTCAT CACCAAGACC ATCAAGTTTG TCAAGTTCTT GGACCACGCC ATCGCGTTCT CGCAGGTACG CTTCTGCCTC ACAGGGCTGC TGGTGATCCT CTATGGGATG CTGCTCCTCG TGGAGGTCAA TGTCATCAGG GTGAGGAGAT ACATCTTCTT CAAGACACCG AGGGAGGTGA AGCCTCCCGA GGACCTGCAA GACCTGGGGG TACGCTTCCT GCAGCCCTTC GTGAATCTGC TGTCCAAAGG CACCTACTGG TGGATGAACG CCTTCATCAA GACTGCCCAC AAGAAGCCCA TCGACTTGCG AGCCATCGGG AAGCTGCCCA TCGCCATGAG GGCCCTCACC AACTACCAAC GGCTCTGCGA GGCCTTTGAC GCCCAGGTGC GGAAGGACAT TCAGGGCACT CAAGGTGCCC GGGCCATCTG GCAGGCACTC AGCCATGCCT TCGGGAGGCG CCTGGTCCTC AGCAGCACTT TCCGCATCTT GGCCGACCTG CTGGGCTTCG CCGGGCCACT GTGCATCTTT GGGATCGTGG ACCACCTTGG GAAGGAGAAC GACGTCTTCC AGCCCAAGAC ACAATTTCTC GGGGTTTACT TTGTCTCATC CCAAGAGTTC CTTGCCAATG CCTACGTCTT AGCTGTGCTT CTGTTCCTTG CCCTCCTACT GCAAAGGACA TTTCTGCAAG CATCCTACTA TGTGGCCATT GAAACTGGAA TTAACTTGAG AGGAGCAATA CAGACCAAGA TTTACAATAA AATTATGCAC CTGTCCACCT CCAACCTGTC CATGGGAGAA ATGACTGCTG GACAGATCTG TAATCTGGTT GCCATCGACA CCAATCAGCT CATGTGGTTT TTCTTCTTGT GCCCAAACCT CTGGGCTATG CCAGTACAGA TCATTGTGGG TGTGATTCTC CTCTACTACA TACTCGGAGT CAGTGCCTTA ATTGGAGCAG CTGTCATCAT TCTACTGGCT CCTGTCCAGT ACTTCGTGGC CACCAAGCTG TCTCAGGCCC AGCGGACGAC ACTGGAGTAT TCCAATGAGC GGCTGAAGCA GACCAACGAG ATGCTCCGCG GCATCAAGCT GCTGAAGCTG TACGCCTGGG AGAACATCTT CCGCACGCGG GTGGAGACGA CCCGCAGGAA GGAGATGACC AGCCTCAGGG CCTTTGCCAT CTATACCTCC ATCTCCATTT TCATGAACAC GGCCATCCCC ATTGCAGCTG TCCTCATAAC TTTCGTGGGC CATGTCAGCT TCTTCAAAGA GGCCGACTTC TCGCCCTCCG TGGCCTTTGC CTCCCTCTCC CTCTTCCATA TCTTGGTCAC ACCGCTGTTC CTGCTGTCCA GTGTGGTCCG ATCTACCGTC AAAGCTCTAG TGAGCGTGCA AAAGCTAAGC GAGTTCCTGT CCAGTGCAGA GATCCGTGAG GAGCAGTGTG CCCCCCATGA GCCCACACCT CAGGGCCCAG CCAGCAAGTA CCAGGCGGTG CCCCTCAGGG TTGTGAACCG CAAGCGTCCA GCCCGGGAGG ATTGTCGGGG CCTCACCGGC CCACTGCAGA GCCTGGTCCC CAGTGCAGAT GGCGATGCTG ACAACTGCTG TGTCCAGATC ATGGGAGGCT ACTTCACGTG GACCCCAGAT GGAATCCCCA CACTGTCCAA CATCACCATT CGTATCCCCC GAGGCCAGCT GACTATGATC GTGGGGCAGG TGGGCTGCGG CAAGTCCTCG CTCCTTCTAG CCGCACTGGG GGAGATGCAG AAGGTCTCAG GGGCTGTCTT CTGGAGCAGC CTTCCTGACA GCGAGATAGG AGAGGACCCC AGCCCAGAGC GGGAGACAGC GACCGACTTG GATATCAGGA AGAGAGGCCC CGTGGCCTAT GCTTCGCAGA AACCATGGCT GCTAAATGCC ACTGTGGAGG AGAACATCAT CTTTGAGAGT CCCTTCAACA AACAACGGTA CAAGATGGTC ATTGAAGCCT GCTCTCTGCA GCCAGACATC GACATCCTGC CCCATGGAGA CCAGACCCAG ATTGGGGAAC GGGGCATCAA CCTGTCTGGT GGTCAACGCC AGCGAATCAG TGTGGCCCGA GCCCTCTACC AGCACGCCAA CGTTGTCTTC TTGGATGACC CCTTCTCAGC TCTGGATATC CATCTGAGTG ACCACTTAAT GCAGGCCGGC ATCCTTGAGC TGCTCCGGGA CGACAAGAGG ACAGTGGTCT TAGTGACCCA CAAGCTACAG TACCTGCCCC ATGCAGACTG GATCATTGCC ATGAAGGATG GCACCATCCA GAGGGAGGGT ACCCTCAAGG ACTTCCAGAG GTCTGAATGC CAGCTCTTTG AGCACTGGAA GACCCTCATG AACCGACAGG ACCAAGAGCT GGAGAAGGAG ACTGTCACAG AGAGAAAAGC CACAGAGCCA CCCCAGGGCC TATCTCGTGC CATGTCCTCG AGGGATGGCC TTCTGCAGGA TGAGGAAGAG GAGGAAGAGG AGGCAGCTGA GAGCGAGGAG GATGACAACC TGTCGTCCAT GCTGCACCAG CGTGCTGAGA TCCCATGGCG AGCCTGCGCC AAGTACCTGT CCTCCGCCGG CATCCTGCTC CTGTCGTTGC TGGTCTTCTC ACAGCTGCTC AAGCACATGG TCCTGGTGGC CATCGACTAC TGGCTGGCCA AGTGGACCGA CAGCGCCCTG ACCCTGACCC CTGCAGCCAG GAACTGCTCC CTCAGCCAGG AGTGCACCCT CGACCAGACT GTCTATGCCA TGGTGTTCAC GGCTGTCTGC AGCCTGGGCA TTGTGCTGTG CCTCGTCACG TCTGTCACTG TGGAGTGGAC AGGGCTGAAG GTGGCCAAGA GACTGCACCG CAGCCTGCTA AACCGGATCA TCCTAGCCCC CATGAGGTTT TTTGAGACCA CGCCCCTTGG GAGCATCCTG AACAGATTTT CATCTGACTG TAACACCATC GACCAGCACA TCCCATCCAC GCTGGAGTGC CTGAGCCGCT CCACCCTGCT CTGTGTCTCA GCCCTGGCCG TCATCTCCTA TGTCACACCT GTGTTCCTCG TGGCCCTCTT GCCCCTGGCC ATCGTGTGCT ACTTCATCCA GAAGTACTTC CGGGTGGCGT CCAGGGACCT GCAGCAGCTG GATGACACCA CCCAGCTTCC ACTTCTCTCA CACTTTGCCG AAACCGTAGA AGGACTCACC ACCATCCGGG CCTTCAGGTA TGAGGCCCGG TTCCAGCAGA AGCTTCTCGA ATACACAGAC TCCAACAACA TTGCTTCCCT CTTCCTCACA GCTGCCAACA GATGGCTGGA AGTCCGAATG GAGTACATCG GTGCATGTGT GGTGCTCATC GCAGCGGTGA CCTCCATCTC CAACTCCCTG CACAGGGAGC TCTCTGCTGG CCTGGTGGGC CTGGGCCTTA CCTACGCCCT AATGGTCTCC AACTACCTCA ACTGGATGGT GAGGAACCTG GCAGACATGG AGCTCCAGCT GGGGGCTGTG AAGCGCATCC ATGGGCTCCT GAAAACCGAG GCAGAGAGCT ACGAGGGACT CCTGGCACCA TCGCTGATCC CAAAGAACTG GCCAGACCAA GGGAAGATCC AGATCCAGAA CCTGAGCGTG CGCTACGACA GCTCCCTGAA GCCGGTGCTG AAGCACGTCA ATGCCCTCAT CTCCCCTGGA CAGAAGATCG GGATCTGCGG CCGCACCGGC AGTGGGAAGT CCTCCTTCTC TCTTGCCTTC TTCCGCATGG TGGACACGTT CGAAGGGCAC ATCATCATTG ATGGCATTGA CATCGCCAAA CTGCCGCTGC ACACCCTGCG CTCACGCCTC TCCATCATCC TGCAGGACCC CGTCCTCTTC AGCGGCACCA TCCGATTTAA CCTGGACCCT GAGAGGAAGT GCTCAGATAG CACACTGTGG GAGGCCCTGG AAATCGCCCA GCTGAAGCTG GTGGTGAAGG CACTGCCAGG AGGCCTCGAT GCCATCATCA CAGAAGGCGG GGAGAATTTC AGCCAGGGAC AGAGGCAGCT GTTCTGCCTG GCCCGGGCCT TCGTGAGGAA GACCAGCATC TTCATCATGG ACGAGGCCAC GGCTTCCATT GACATGGCCA CGGAAAACAT CCTCCAAAAG GTGGTGATGA CAGCCTTCGC AGACCGCACT GTGGTCACCA TCGCGCATCG AGTGCACACC ATCCTGAGTG CAGACCTGGT GATCGTCCTG AAGCGGGGTG CCATCCTTGA GTTCGATAAG CCAGAGAAGC TGCTCAGCCG GAAGGACAGC GTCTTCGCCT CCTTCGTCCG TGCAGACAAG AAGCTTCATC ATCACCATCA CCATTAGGAT CCACCGGATC TAGATAACTG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTAACG CGTAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT CCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAG ATCGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC TAGGGGGAGG CTAACTGAAA CACGGAAGGA GACAATACCG GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCCTCAGGT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCATGCAT
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