pAAV-CamKII-Pdp1(mouse)-2×HA
$70.00
In stock
SKU
PL40329
NM_001033453.3;Gm1024; Ppm2c
100μg liquid plasmid(Instantly):$91
| name | pAAV-CamKII-Pdp1(mouse)-2×HA | another name | apAAV-CamKII-Pdp1(mouse)-2×HA |
| Insert Size (bp) | 1617bp | Plasmid host | Mammary cell,Adeno-associated virus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CaMKIIa | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTTTAAC ATTATGGCCT TAGGTCACTT CATCTCCATG GGGTTCTTCT TCTGATTTTC TAGAAAATGA GATGGGGGTG CAGAGAGCTT CCTCAGTGAC CTGCCCAGGG TCACATCAGA AATGTCAGAG CTAGAACTTG AACTCAGATT ACTAATCTTA AATTCCATGC CTTGGGGGCA TGCAAGTACG ATATACAGAA GGAGTGAACT CATTAGGGCA GATGACCAAT GAGTTTAGGA AAGAAGAGTC CAGGGCAGGG TACATCTACA CCACCCGCCC AGCCCTGGGT GAGTCCAGCC ACGTTCACCT CATTATAGTT GCCTCTCTCC AGTCCTACCT TGACGGGAAG CACAAGCAGA AACTGGGACA GGAGCCCCAG GAGACCAAAT CTTCATGGTC CCTCTGGGAG GATGGGTGGG GAGAGCTGTG GCAGAGGCCT CAGGAGGGGC CCTGCTGCTC AGTGGTGACA GATAGGGGTG AGAAAGCAGA CAGAGTCATT CCGTCAGCAT TCTGGGTCTG TTTGGTACTT CTTCTCACGC TAAGGTGGCG GTGTGATATG CACAATGGCT AAAAAGCAGG GAGAGCTGGA AAGAAACAAG GACAGAGACA GAGGCCAAGT CAACCAGACC AATTCCCAGA GGAAGCAAAG AAACCATTAC AGAGACTACA AGGGGGAAGG GAAGGAGAGA TGAATTAGCT TCCCCTGTAA ACCTTAGAAC CCAGCTGTTG CCAGGGCAAC GGGGCAATAC CTGTCTCTTC AGAGGAGATG AAGTTGCCAG GGTAACTACA TCCTGTCTTT CTCAAGGACC ATCCCAGAAT GTGGCACCCA CTAGCCGTTA CCATAGCAAC TGCCTCTTTG CCCCACTTAA TCCCATCCCG TCTGTTAAAA GGGCCCTATA GTTGGAGGTG GGGGAGGTAG GAAGAGCGAT GATCACTTGT GGACTAAGTT TGTTCGCATC CCCTTCTCCA ACCCCCTCAG TACATCACCC TGGGGGAACA GGGTCCACTT GCTCCTGGGC CCACACAGTC CTGCAGTATT GTGTATATAA GGCCAGGGCA AAGAGGAGCA GGTTTTAAAG TGAAAGGCAG GCAGGTGTTG GGGAGGCAGT TACCGGGGCA ACGGGAACAG GGCGTTTCGG AGGTGGTTGC CATGGGGACC TGGATGCTGA CGAAGGCTCG CGAGGCTGTG AGCAGCCACA GTGCCCTGCT CAGAAGCCCC AAGCTCGTCA GTCAAGCCGG TTCTCCGTTT GCACTCAGGA GCACGGGCAG GCGAGTGGCC CCTAGTTCTG GGGGCAGCTC TAGAGCGGTA CCGGATCCAC TAGTGCCACC ATGCCAGCAC CAACTCAACT GTTTTTTCCT CTCGTCCGTA ACTGTGAACT GAGCAGAATC TATGGCACTG CATGTTACTG CCACCACAAA CATCTCTGCT GTTCACCACC ATACATTCCT CAGAATCGTC TGAGGTACAC ACCCCATCCT GCATATGCTA CCTTTTGTAG GCCACGGGAG AACTGGTGGC AGTATACTCA AGGAAGGAGA TACGCTTCTA CACCGCAGAA ATTTTACCTC ACACCTCCAC AAGTCAACAG CATCCTTAAA GCTAATGAAT ACAGCTTCAA AGTACCAGAA TTTGATGGCA AAAATGTCAG TTCCATTCTT GGATTTGACA GCAATCAGCT GCCTGCAAAT GCACCCATAG AGGACCGGAG AAGTGCAGCA ACCTGCTTGC AGACCAGAGG GATGCTCTTG GGGGTTTTTG ATGGTCATGC AGGCTGTGCT TGTTCCCAGG CAGTCAGTGA AAGACTCTTC TATTATATTG CTGTTTCCTT GTTGCCCCAT GAGACTTTGC TAGAGATTGA AAATGCAGTG GAGAGTGGTC GGGCACTGCT ACCTATCCTT CAGTGGCACA AGCACCCCAA TGATTACTTC AGTAAGGAGG CGTCCAAATT GTATTTCAAC AGCTTGAGGA CTTACTGGCA AGAACTCATA GACCTCAATA CTGGAGAATC AGCTGATATT GATGTTAAGG AGGCTTTAAT TAATGCTTTC AAGAGACTCG ATAATGACAT TTCATTGGAG GCTCAAGTTG GTGATCCTAA TTCTTTTCTA AATTACCTGG TGCTTCGGGT AGCATTTTCT GGGGCTACTG CTTGTGTGGC CCATGTAGAT GGTGTTGACC TCCATGTGGC TAACACTGGT GATAGTAGAG CCATGCTAGG TGTGCAAGAA GAAGATGGCT CTTGGTCAGC AGTCACACTC TCTAATGACC ACAATGCTCA GAATGAAAGA GAACTAGAGC GTCTGAAACT GGAACACCCA AAAAATGAGG CCAAGAGCGT GGTAAAGCAG GACCGGCTGC TTGGCTTGCT GATGCCCTTT AGGGCTTTTG GAGATGTAAA GTTCAAATGG AGCATTGACC TTCAAAAGAG AGTGATAGAG TCTGGCCCAG ACCAGTTGAA TGACAATGAA TACACCAAGT TTATCCCTCC TAACTATCAT ACACCTCCTT ATCTTACTGC TGAGCCAGAG GTAACTTATC ACAGATTAAG GCCACAGGAT AAATTCCTAG TGTTAGCAAC TGATGGGTTG TGGGAGACTA TGCATAGACA GGATGTGGTT AGGATTGTGG GTGAATACTT AACTGGTATG CATCACCAAC AGCCAATAGC AGTTGGTGGG TACAAGGTGA CTCTGGGACA GATGCATGGC CTTTTAACAG AAAGGAGAGC AAAGATGTCA TCAGTGTTTG AGGATCAGAA TGCAGCAACC CATCTCATTC GCCATGCTGT AGGCAATAAT GAATTTGGGG CTGTTGATCA TGAACGACTC TCTAAAATGC TTAGTCTTCC TGAAGAGCTT GCTCGGATGT ATAGAGATGA CATTACAATC ATTGTAGTTC AGTTCAATTC TCATGTTGTA GGGGCATACC AAAACCAGGA ACAGGCTAGC TACCCATACG ACGTACCTGA TTATGCATCA TACCCATACG ATGTTCCAGA TTACGCTTAG TGAATTCACC GGTTAGTAAG CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTATGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT CGATACCGAG CGCTGCTCGA GAGATCTACG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTGTAGGT AACCACGTGC GGACCGAGCG GCCGCAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGCTC GCTCGCTCAC TGAGGCCGGG CGACCAAAGG TCGCCCGACG CCCGGGCTTT GCCCGGGCGG CCTCAGTGAG CGAGCGAGCG CGCAGCTGCC TGCAGGGGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TACGTCAAAG CAACCATAGT ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTTGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGGCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTTATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
