pHSE401-AtU6-26-RbohD(Chrysanthemum)-sgRNA1-sgRNA2-Cas9
$99.00
In stock
SKU
PL40326
100μg liquid plasmid(Instantly):$142
| name | pHSE401-AtU6-26-RbohD(Chrysanthemum)-sgRNA1-sgRNA2-Cas9 | another name | apHSE401-AtU6-26-RbohD(Chrysanthemum)-sgRNA1-sgRNA2-Cas9 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | AtU6 | Replicon | pVS1 |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAAACGCTCT TTTCTCTTAG GTTTACCCGC CAATATATCC TGTCAAACAC TGATAGTTTA AACTGAAGGC GGGAAACGAC AATCTGATCC AAGCTCAAGC TGCTCTAGCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGCCAAG CTTCGACTTG CCTTCCGCAC AATACATCAT TTCTTCTTAG CTTTTTTTCT TCTTCTTCGT TCATACAGTT TTTTTTTGTT TATCAGCTTA CATTTTCTTG AACCGTAGCT TTCGTTTTCT TCTTTTTAAC TTTCCATTCG GAGTTTTTGT ATCTTGTTTC ATAGTTTGTC CCAGGATTAG AATGATTAGG CATCGAACCT TCAAGAATTT GATTGAATAA AACATCTTCA TTCTTAAGAT ATGAAGATAA TCTTCAAAAG GCCCCTGGGA ATCTGAAAGA AGAGAAGCAG GCCCATTTAT ATGGGAAAGA ACAATAGTAT TTCTTATATA GGCCCATTTA AGTTGAAAAC AATCTTCAAA AGTCCCACAT CGCTTAGATA AGAAAACGAA GCTGAGTTTA TATACAGCTA GAGTCGAAGT AGTGATTGTA CTAGGTCAAG AAACACCGGT TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG CACCGAGTCG GTGCGGTATG ACCGGACTAA GTCTGGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC CGAGTCGGTG CTTTTTTTTG CAAAATTTTC CAGATCGATT TCTTCTTCCT CTGTTCTTCG GCGTTCAATT TCTGGGGTTT TCTCTTCGTT TTCTGTAACT GAAACCTAAA ATTTGACCTA AAAAAAATCT CAAATAATAT GATTCAGTGG TTTTGTACTT TTCAGTTAGT TGAGTTTTGC AGTTCCGATG AGATAAACCA ATAAGCTTGC ATGCCTGCAG GTCAACATGG TGGAGCACGA CACACTTGTC TACTCCAAAA ATATCAAAGA TACAGTCTCA GAAGACCAAA GGGCAATTGA GACTTTTCAA CAAAGGGTAA TATCCGGAAA CCTCCTCGGA TTCCATTGCC CAGCTATCTG TCACTTTATT GTGAAGATAG TGGAAAAGGA AGGTGGCTCC TACAAATGCC ATCATTGCGA TAAAGGAAAG GCCATCGTTG AAGATGCCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC ACCCACGAGG AGCATCGTGG AAAAAGAAGA CGTTCCAACC ACGTCTTCAA AGCAAGTGGA TTGATGTGAT AACATGGTGG AGCACGACAC ACTTGTCTAC TCCAAAAATA TCAAAGATAC AGTCTCAGAA GACCAAAGGG CAATTGAGAC TTTTCAACAA AGGGTAATAT CCGGAAACCT CCTCGGATTC CATTGCCCAG CTATCTGTCA CTTTATTGTG AAGATAGTGG AAAAGGAAGG TGGCTCCTAC AAATGCCATC ATTGCGATAA AGGAAAGGCC ATCGTTGAAG ATGCCTCTGC CGACAGTGGT CCCAAAGATG GACCCCCACC CACGAGGAGC ATCGTGGAAA AAGAAGACGT TCCAACCACG TCTTCAAAGC AAGTGGATTG ATGTGATATC TCCACTGACG TAAGGGATGA CGCACAATCC CACTATCCTT CGCAAGACCC TTCCTCTATA TAAGGAAGTT CATTTCATTT GGAGAGGACC TCGACCTCAA CACAACATAT ACAAAACAAA CGAATCTCAA GCAATCAAGC ATTCTACTTC TATTGCAGCA ATTTAAATCA TTTCTTTTAA AGCAAAAGCA ATTTTCTGAA AATTTTCACC ATTTACGAAC GATACTCGAG TAATCTAGAT GGATTACAAG GACCACGACG GGGATTACAA GGACCACGAC ATTGATTACA AGGATGATGA TGACAAGATG GCTCCGAAGA AGAAGAGGAA GGTTGGCATC CACGGGGTGC CAGCTGCTGA CAAGAAGTAC TCGATCGGCC TCGATATTGG GACTAACTCT GTTGGCTGGG CCGTGATCAC CGACGAGTAC AAGGTGCCCT CAAAGAAGTT CAAGGTCCTG GGCAACACCG ATCGGCATTC CATCAAGAAG AATCTCATTG GCGCTCTCCT GTTCGACAGC GGCGAGACGG CTGAGGCTAC GCGGCTCAAG CGCACCGCCC GCAGGCGGTA CACGCGCAGG AAGAATCGCA TCTGCTACCT GCAGGAGATT TTCTCCAACG AGATGGCGAA GGTTGACGAT TCTTTCTTCC ACAGGCTGGA GGAGTCATTC CTCGTGGAGG AGGATAAGAA GCACGAGCGG CATCCAATCT TCGGCAACAT TGTCGACGAG GTTGCCTACC ACGAGAAGTA CCCTACGATC TACCATCTGC GGAAGAAGCT CGTGGACTCC ACAGATAAGG CGGACCTCCG CCTGATCTAC CTCGCTCTGG CCCACATGAT TAAGTTCAGG GGCCATTTCC TGATCGAGGG GGATCTCAAC CCGGACAATA GCGATGTTGA CAAGCTGTTC ATCCAGCTCG TGCAGACGTA CAACCAGCTC TTCGAGGAGA ACCCCATTAA TGCGTCAGGC GTCGACGCGA AGGCTATCCT GTCCGCTAGG CTCTCGAAGT CTCGGCGCCT CGAGAACCTG ATCGCCCAGC TGCCGGGCGA GAAGAAGAAC GGCCTGTTCG GGAATCTCAT TGCGCTCAGC CTGGGGCTCA CGCCCAACTT CAAGTCGAAT TTCGATCTCG CTGAGGACGC CAAGCTGCAG CTCTCCAAGG ACACATACGA CGATGACCTG GATAACCTCC TGGCCCAGAT CGGCGATCAG TACGCGGACC TGTTCCTCGC TGCCAAGAAT CTGTCGGACG CCATCCTCCT GTCTGATATT CTCAGGGTGA ACACCGAGAT TACGAAGGCT CCGCTCTCAG CCTCCATGAT CAAGCGCTAC GACGAGCACC ATCAGGATCT GACCCTCCTG AAGGCGCTGG TCAGGCAGCA GCTCCCCGAG AAGTACAAGG AGATCTTCTT CGATCAGTCG AAGAACGGCT ACGCTGGGTA CATTGACGGC GGGGCCTCTC AGGAGGAGTT CTACAAGTTC ATCAAGCCGA TTCTGGAGAA GATGGACGGC ACGGAGGAGC TGCTGGTGAA GCTCAATCGC GAGGACCTCC TGAGGAAGCA GCGGACATTC GATAACGGCA GCATCCCACA CCAGATTCAT CTCGGGGAGC TGCACGCTAT CCTGAGGAGG CAGGAGGACT TCTACCCTTT CCTCAAGGAT AACCGCGAGA AGATCGAGAA GATTCTGACT TTCAGGATCC CGTACTACGT CGGCCCACTC GCTAGGGGCA ACTCCCGCTT CGCTTGGATG ACCCGCAAGT CAGAGGAGAC GATCACGCCG TGGAACTTCG AGGAGGTGGT CGACAAGGGC GCTAGCGCTC AGTCGTTCAT CGAGAGGATG ACGAATTTCG ACAAGAACCT GCCAAATGAG AAGGTGCTCC CTAAGCACTC GCTCCTGTAC GAGTACTTCA CAGTCTACAA CGAGCTGACT AAGGTGAAGT ATGTGACCGA GGGCATGAGG AAGCCGGCTT TCCTGTCTGG GGAGCAGAAG AAGGCCATCG TGGACCTCCT GTTCAAGACC AACCGGAAGG TCACGGTTAA GCAGCTCAAG GAGGACTACT TCAAGAAGAT TGAGTGCTTC GATTCGGTCG AGATCTCTGG CGTTGAGGAC CGCTTCAACG CCTCCCTGGG GACCTACCAC GATCTCCTGA AGATCATTAA GGATAAGGAC TTCCTGGACA ACGAGGAGAA TGAGGATATC CTCGAGGACA TTGTGCTGAC ACTCACTCTG TTCGAGGACC GGGAGATGAT CGAGGAGCGC CTGAAGACTT ACGCCCATCT CTTCGATGAC AAGGTCATGA AGCAGCTCAA GAGGAGGAGG TACACCGGCT GGGGGAGGCT GAGCAGGAAG CTCATCAACG GCATTCGGGA CAAGCAGTCC GGGAAGACGA TCCTCGACTT CCTGAAGAGC GATGGCTTCG CGAACCGCAA TTTCATGCAG CTGATTCACG ATGACAGCCT CACATTCAAG GAGGATATCC AGAAGGCTCA GGTGAGCGGC CAGGGGGACT CGCTGCACGA GCATATCGCG AACCTCGCTG GCTCGCCAGC TATCAAGAAG GGGATTCTGC AGACCGTGAA GGTTGTGGAC GAGCTGGTGA AGGTCATGGG CAGGCACAAG CCTGAGAACA TCGTCATTGA GATGGCCCGG GAGAATCAGA CCACGCAGAA GGGCCAGAAG AACTCACGCG AGAGGATGAA GAGGATCGAG GAGGGCATTA AGGAGCTGGG GTCCCAGATC CTCAAGGAGC ACCCGGTGGA GAACACGCAG CTGCAGAATG AGAAGCTCTA CCTGTACTAC CTCCAGAATG GCCGCGATAT GTATGTGGAC CAGGAGCTGG ATATTAACAG GCTCAGCGAT TACGACGTCG ATCATATCGT TCCACAGTCA TTCCTGAAGG ATGACTCCAT TGACAACAAG GTCCTCACCA GGTCGGACAA GAACCGGGGC AAGTCTGATA ATGTTCCTTC AGAGGAGGTC GTTAAGAAGA TGAAGAACTA CTGGCGCCAG CTCCTGAATG CCAAGCTGAT CACGCAGCGG AAGTTCGATA ACCTCACAAA GGCTGAGAGG GGCGGGCTCT CTGAGCTGGA CAAGGCGGGC TTCATCAAGA GGCAGCTGGT CGAGACACGG CAGATCACTA AGCACGTTGC GCAGATTCTC GACTCACGGA TGAACACTAA GTACGATGAG AATGACAAGC TGATCCGCGA GGTGAAGGTC ATCACCCTGA AGTCAAAGCT CGTCTCCGAC TTCAGGAAGG ATTTCCAGTT CTACAAGGTT CGGGAGATCA ACAATTACCA CCATGCCCAT GACGCGTACC TGAACGCGGT GGTCGGCACA GCTCTGATCA AGAAGTACCC AAAGCTCGAG AGCGAGTTCG TGTACGGGGA CTACAAGGTT TACGATGTGA GGAAGATGAT CGCCAAGTCG GAGCAGGAGA TTGGCAAGGC TACCGCCAAG TACTTCTTCT ACTCTAACAT TATGAATTTC TTCAAGACAG AGATCACTCT GGCCAATGGC GAGATCCGGA AGCGCCCCCT CATCGAGACG AACGGCGAGA CGGGGGAGAT CGTGTGGGAC AAGGGCAGGG ATTTCGCGAC CGTCAGGAAG GTTCTCTCCA TGCCACAAGT GAATATCGTC AAGAAGACAG AGGTCCAGAC TGGCGGGTTC TCTAAGGAGT CAATTCTGCC TAAGCGGAAC AGCGACAAGC TCATCGCCCG CAAGAAGGAC TGGGATCCGA AGAAGTACGG CGGGTTCGAC AGCCCCACTG TGGCCTACTC GGTCCTGGTT GTGGCGAAGG TTGAGAAGGG CAAGTCCAAG AAGCTCAAGA GCGTGAAGGA GCTGCTGGGG ATCACGATTA TGGAGCGCTC CAGCTTCGAG AAGAACCCGA TCGATTTCCT GGAGGCGAAG GGCTACAAGG AGGTGAAGAA GGACCTGATC ATTAAGCTCC CCAAGTACTC ACTCTTCGAG CTGGAGAACG GCAGGAAGCG GATGCTGGCT TCCGCTGGCG AGCTGCAGAA GGGGAACGAG CTGGCTCTGC CGTCCAAGTA TGTGAACTTC CTCTACCTGG CCTCCCACTA CGAGAAGCTC AAGGGCAGCC CCGAGGACAA CGAGCAGAAG CAGCTGTTCG TCGAGCAGCA CAAGCATTAC CTCGACGAGA TCATTGAGCA GATTTCCGAG TTCTCCAAGC GCGTGATCCT GGCCGACGCG AATCTGGATA AGGTCCTCTC CGCGTACAAC AAGCACCGCG ACAAGCCAAT CAGGGAGCAG GCTGAGAATA TCATTCATCT CTTCACCCTG ACGAACCTCG GCGCCCCTGC TGCTTTCAAG TACTTCGACA CAACTATCGA TCGCAAGAGG TACACAAGCA CTAAGGAGGT CCTGGACGCG ACCCTCATCC ACCAGTCGAT TACCGGCCTC TACGAGACGC GCATCGACCT GTCTCAGCTC GGGGGCGACA AGCGGCCAGC GGCGACGAAG AAGGCGGGGC AGGCGAAGAA GAAGAAGTGA GCTCAGAGCT TTCGTTCGTA TCATCGGTTT CGACAACGTT CGTCAAGTTC AATGCATCAG TTTCATTGCG CACACACCAG AATCCTACTG AGTTTGAGTA TTATGGCATT GGGAAAACTG TTTTTCTTGT ACCATTTGTT GTGCTTGTAA TTTACTGTGT TTTTTATTCG GTTTTCGCTA TCGAACTGTG AAATGGAAAT GGATGGAGAA GAGTTAATGA ATGATATGGT CCTTTTGTTC ATTCTCAAAT TAATATTATT TGTTTTTTCT CTTATTTGTT GTGTGTTGAA TTTGAAATTA TAAGAGATAT GCAAACATTT TGTTTTGAGT AAAAATGTGT CAAATCGTGG CCTCTAATGA CCGAAGTTAA TATGAGGAGT AAAACACTTG TAGTTGTACC ATTATGCTTA TTCACTAGGC AACAAATATA TTTTCAGACC TAGAAAAGCT GCAAATGTTA CTGAATACAA GTATGTCCTC TTGTGTTTTA GACATTTATG AACTTTCCTT TATGTAATTT TCCAGAATCC TTGTCAGATT CTAATCATTG CTTTATAATT ATAGTTATAC TCATGGATTT GTAGTTGAGT ATGAAAATAT TTTTTAATGC ATTTTATGAC TTGCCAATTG ATTGACAACG AATTCGTAAT CATGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGC TAGAGCAGCT TGCCAACATG GTGGAGCACG ACACTCTCGT CTACTCCAAG AATATCAAAG ATACAGTCTC AGAAGACCAA AGGGCTATTG AGACTTTTCA ACAAAGGGTA ATATCGGGAA ACCTCCTCGG ATTCCATTGC CCAGCTATCT GTCACTTCAT CAAAAGGACA GTAGAAAAGG AAGGTGGCAC CTACAAATGC CATCATTGCG ATAAAGGAAA GGCTATCGTT CAAGATGCCT CTGCCGACAG TGGTCCCAAA GATGGACCCC CACCCACGAG GAGCATCGTG GAAAAAGAAG ACGTTCCAAC CACGTCTTCA AAGCAAGTGG ATTGATGTGA ACATGGTGGA GCACGACACT CTCGTCTACT CCAAGAATAT CAAAGATACA GTCTCAGAAG ACCAAAGGGC TATTGAGACT TTTCAACAAA GGGTAATATC GGGAAACCTC CTCGGATTCC ATTGCCCAGC TATCTGTCAC TTCATCAAAA GGACAGTAGA AAAGGAAGGT GGCACCTACA AATGCCATCA TTGCGATAAA GGAAAGGCTA TCGTTCAAGA TGCCTCTGCC GACAGTGGTC CCAAAGATGG ACCCCCACCC ACGAGGAGCA TCGTGGAAAA AGAAGACGTT CCAACCACGT CTTCAAAGCA AGTGGATTGA TGTGATATCT CCACTGACGT AAGGGATGAC GCACAATCCC ACTATCCTTC GCAAGACCCT TCCTCTATAT AAGGAAGTTC ATTTCATTTG GAGAGGACAC GCTGAAATCA CCAGTCTCTC TCTACAAATC TATCTCTCTC GAGCTTTCGC AGATCCGGGG GGCAATGAGA TATGAAAAAG CCTGAACTCA CCGCGACGTC TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTCTCC GACCTGATGC AGCTCTCGGA GGGCGAAGAA TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG CGTGGATATG TCCTGCGGGT AAATAGCTGC GCCGATGGTT TCTACAAAGA TCGTTATGTT TATCGGCACT TTGCATCGGC CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAGTTT AGCGAGAGCC TGACCTATTG CATCTCCCGC CGTTCACAGG GTGTCACGTT GCAAGACCTG CCTGAAACCG AACTGCCCGC TGTTCTACAA CCGGTCGCGG AGGCTATGGA TGCGATCGCT GCGGCCGATC TTAGCCAGAC GAGCGGGTTC GGCCCATTCG GACCGCAAGG AATCGGTCAA TACACTACAT GGCGTGATTT CATATGCGCG ATTGCTGATC CCCATGTGTA TCACTGGCAA ACTGTGATGG ACGACACCGT CAGTGCGTCC GTCGCGCAGG CTCTCGATGA GCTGATGCTT TGGGCCGAGG ACTGCCCCGA AGTCCGGCAC CTCGTGCACG CGGATTTCGG CTCCAACAAT GTCCTGACGG ACAATGGCCG CATAACAGCG GTCATTGACT GGAGCGAGGC GATGTTCGGG GATTCCCAAT ACGAGGTCGC CAACATCTTC TTCTGGAGGC CGTGGTTGGC TTGTATGGAG CAGCAGACGC GCTACTTCGA GCGGAGGCAT CCGGAGCTTG CAGGATCGCC ACGACTCCGG GCGTATATGC TCCGCATTGG TCTTGACCAA CTCTATCAGA GCTTGGTTGA CGGCAATTTC GATGATGCAG CTTGGGCGCA GGGTCGATGC GACGCAATCG TCCGATCCGG AGCCGGGACT GTCGGGCGTA CACAAATCGC CCGCAGAAGC GCGGCCGTCT GGACCGATGG CTGTGTAGAA GTACTCGCCG ATAGTGGAAA CCGACGCCCC AGCACTCGTC CGAGGGCAAA GAAATAGAGT AGATGCCGAC CGGGATCTGT CGATCGACAA GCTCGAGTTT CTCCATAATA ATGTGTGAGT AGTTCCCAGA TAAGGGAATT AGGGTTCCTA TAGGGTTTCG CTCATGTGTT GAGCATATAA GAAACCCTTA GTATGTATTT GTATTTGTAA AATACTTCTA TCAATAAAAT TTCTAATTCC TAAAACCAAA ATCCAGTACT AAAATCCAGA TCCCCCGAAT TAATTCGGCG TTAATTCAGT ACATTAAAAA CGTCCGCAAT GTGTTATTAA GTTGTCTAAG CGTCAATTTG TTTACACCAC AATATATCCT GCCACCAGCC AGCCAACAGC TCCCCGACCG GCAGCTCGGC ACAAAATCAC CACTCGATAC AGGCAGCCCA TCAGTCCGGG ACGGCGTCAG CGGGAGAGCC GTTGTAAGGC GGCAGACTTT GCTCATGTTA CCGATGCTAT TCGGAAGAAC GGCAACTAAG CTGCCGGGTT TGAAACACGG ATGATCTCGC GGAGGGTAGC ATGTTGATTG TAACGATGAC AGAGCGTTGC TGCCTGTGAT CACCGCGGTT TCAAAATCGG CTCCGTCGAT ACTATGTTAT ACGCCAACTT TGAAAACAAC TTTGAAAAAG CTGTTTTCTG GTATTTAAGG TTTTAGAATG CAAGGAACAG TGAATTGGAG TTCGTCTTGT TATAATTAGC TTCTTGGGGT ATCTTTAAAT ACTGTAGAAA AGAGGAAGGA AATAATAAAT GGCTAAAATG AGAATATCAC CGGAATTGAA AAAACTGATC GAAAAATACC GCTGCGTAAA AGATACGGAA GGAATGTCTC CTGCTAAGGT ATATAAGCTG GTGGGAGAAA ATGAAAACCT ATATTTAAAA ATGACGGACA GCCGGTATAA AGGGACCACC TATGATGTGG AACGGGAAAA GGACATGATG CTATGGCTGG AAGGAAAGCT GCCTGTTCCA AAGGTCCTGC ACTTTGAACG GCATGATGGC TGGAGCAATC TGCTCATGAG TGAGGCCGAT GGCGTCCTTT GCTCGGAAGA GTATGAAGAT GAACAAAGCC CTGAAAAGAT TATCGAGCTG TATGCGGAGT GCATCAGGCT CTTTCACTCC ATCGACATAT CGGATTGTCC CTATACGAAT AGCTTAGACA GCCGCTTAGC CGAATTGGAT TACTTACTGA ATAACGATCT GGCCGATGTG GATTGCGAAA ACTGGGAAGA AGACACTCCA TTTAAAGATC CGCGCGAGCT GTATGATTTT TTAAAGACGG AAAAGCCCGA AGAGGAACTT GTCTTTTCCC ACGGCGACCT GGGAGACAGC AACATCTTTG TGAAAGATGG CAAAGTAAGT GGCTTTATTG ATCTTGGGAG AAGCGGCAGG GCGGACAAGT GGTATGACAT TGCCTTCTGC GTCCGGTCGA TCAGGGAGGA TATCGGGGAA GAACAGTATG TCGAGCTATT TTTTGACTTA CTGGGGATCA AGCCTGATTG GGAGAAAATA AAATATTATA TTTTACTGGA TGAATTGTTT TAGTACCTAG AATGCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGGGTGC CTTGATGTGG GCGCCGGCGG TCGAGTGGCG ACGGCGCGGC TTGTCCGCGC CCTGGTAGAT TGCCTGGCCG TAGGCCAGCC ATTTTTGAGC GGCCAGCGGC CGCGATAGGC CGACGCGAAG CGGCGGGGCG TAGGGAGCGC AGCGACCGAA GGGTAGGCGC TTTTTGCAGC TCTTCGGCTG TGCGCTGGCC AGACAGTTAT GCACAGGCCA GGCGGGTTTT AAGAGTTTTA ATAAGTTTTA AAGAGTTTTA GGCGGAAAAA TCGCCTTTTT TCTCTTTTAT ATCAGTCACT TACATGTGTG ACCGGTTCCC AATGTACGGC TTTGGGTTCC CAATGTACGG GTTCCGGTTC CCAATGTACG GCTTTGGGTT CCCAATGTAC GTGCTATCCA CAGGAAACAG ACCTTTTCGA CCTTTTTCCC CTGCTAGGGC AATTTGCCCT AGCATCTGCT CCGTACATTA GGAACCGGCG GATGCTTCGC CCTCGATCAG GTTGCGGTAG CGCATGACTA GGATCGGGCC AGCCTGCCCC GCCTCCTCCT TCAAATCGTA CTCCGGCAGG TCATTTGACC CGATCAGCTT GCGCACGGTG AAACAGAACT TCTTGAACTC TCCGGCGCTG CCACTGCGTT CGTAGATCGT CTTGAACAAC CATCTGGCTT CTGCCTTGCC TGCGGCGCGG CGTGCCAGGC GGTAGAGAAA ACGGCCGATG CCGGGATCGA TCAAAAAGTA ATCGGGGTGA ACCGTCAGCA CGTCCGGGTT CTTGCCTTCT GTGATCTCGC GGTACATCCA ATCAGCTAGC TCGATCTCGA TGTACTCCGG CCGCCCGGTT TCGCTCTTTA CGATCTTGTA GCGGCTAATC AAGGCTTCAC CCTCGGATAC CGTCACCAGG CGGCCGTTCT TGGCCTTCTT CGTACGCTGC ATGGCAACGT GCGTGGTGTT TAACCGAATG CAGGTTTCTA CCAGGTCGTC TTTCTGCTTT CCGCCATCGG CTCGCCGGCA GAACTTGAGT ACGTCCGCAA CGTGTGGACG GAACACGCGG CCGGGCTTGT CTCCCTTCCC TTCCCGGTAT CGGTTCATGG ATTCGGTTAG ATGGGAAACC GCCATCAGTA CCAGGTCGTA ATCCCACACA CTGGCCATGC CGGCCGGCCC TGCGGAAACC TCTACGTGCC CGTCTGGAAG CTCGTAGCGG ATCACCTCGC CAGCTCGTCG GTCACGCTTC GACAGACGGA AAACGGCCAC GTCCATGATG CTGCGACTAT CGCGGGTGCC CACGTCATAG AGCATCGGAA CGAAAAAATC TGGTTGCTCG TCGCCCTTGG GCGGCTTCCT AATCGACGGC GCACCGGCTG CCGGCGGTTG CCGGGATTCT TTGCGGATTC GATCAGCGGC CGCTTGCCAC GATTCACCGG GGCGTGCTTC TGCCTCGATG CGTTGCCGCT GGGCGGCCTG CGCGGCCTTC AACTTCTCCA CCAGGTCATC ACCCAGCGCC GCGCCGATTT GTACCGGGCC GGATGGTTTG CGACCGCTCA CGCCGATTCC TCGGGCTTGG GGGTTCCAGT GCCATTGCAG GGCCGGCAGG CAACCCAGCC GCTTACGCCT GGCCAACCGC CCGTTCCTCC ACACATGGGG CATTCCACGG CGTCGGTGCC TGGTTGTTCT TGATTTTCCA TGCCGCCTCC TTTAGCCGCT AAAATTCATC TACTCATTTA TTCATTTGCT CATTTACTCT GGTAGCTGCG CGATGTATTC AGATAGCAGC TCGGTAATGG TCTTGCCTTG GCGTACCGCG TACATCTTCA GCTTGGTGTG ATCCTCCGCC GGCAACTGAA AGTTGACCCG CTTCATGGCT GGCGTGTCTG CCAGGCTGGC CAACGTTGCA GCCTTGCTGC TGCGTGCGCT CGGACGGCCG GCACTTAGCG TGTTTGTGCT TTTGCTCATT TTCTCTTTAC CTCATTAACT CAAATGAGTT TTGATTTAAT TTCAGCGGCC AGCGCCTGGA CCTCGCGGGC AGCGTCGCCC TCGGGTTCTG ATTCAAGAAC GGTTGTGCCG GCGGCGGCAG TGCCTGGGTA GCTCACGCGC TGCGTGATAC GGGACTCAAG AATGGGCAGC TCGTACCCGG CCAGCGCCTC GGCAACCTCA CCGCCGATGC GCGTGCCTTT GATCGCCCGC GACACGACAA AGGCCGCTTG TAGCCTTCCA TCCGTGACCT CAATGCGCTG CTTAACCAGC TCCACCAGGT CGGCGGTGGC CCATATGTCG TAAGGGCTTG GCTGCACCGG AATCAGCACG AAGTCGGCTG CCTTGATCGC GGACACAGCC AAGTCCGCCG CCTGGGGCGC TCCGTCGATC ACTACGAAGT CGCGCCGGCC GATGGCCTTC ACGTCGCGGT CAATCGTCGG GCGGTCGATG CCGACAACGG TTAGCGGTTG ATCTTCCCGC ACGGCCGCCC AATCGCGGGC ACTGCCCTGG GGATCGGAAT CGACTAACAG AACATCGGCC CCGGCGAGTT GCAGGGCGCG GGCTAGATGG GTTGCGATGG TCGTCTTGCC TGACCCGCCT TTCTGGTTAA GTACAGCGAT AACCTTCATG CGTTCCCCTT GCGTATTTGT TTATTTACTC ATCGCATCAT ATACGCAGCG ACCGCATGAC GCAAGCTGTT TTACTCAAAT ACACATCACC TTTTTAGACG GCGGCGCTCG GTTTCTTCAG CGGCCAAGCT GGCCGGCCAG GCCGCCAGCT TGGCATCAGA CAAACCGGCC AGGATTTCAT GCAGCCGCAC GGTTGAGACG TGCGCGGGCG GCTCGAACAC GTACCCGGCC GCGATCATCT CCGCCTCGAT CTCTTCGGTA ATGAAAAACG GTTCGTCCTG GCCGTCCTGG TGCGGTTTCA TGCTTGTTCC TCTTGGCGTT CATTCTCGGC GGCCGCCAGG GCGTCGGCCT CGGTCAATGC GTCCTCACGG AAGGCACCGC GCCGCCTGGC CTCGGTGGGC GTCACTTCCT CGCTGCGCTC AAGTGCGCGG TACAGGGTCG AGCGATGCAC GCCAAGCAGT GCAGCCGCCT CTTTCACGGT GCGGCCTTCC TGGTCGATCA GCTCGCGGGC GTGCGCGATC TGTGCCGGGG TGAGGGTAGG GCGGGGGCCA AACTTCACGC CTCGGGCCTT GGCGGCCTCG CGCCCGCTCC GGGTGCGGTC GATGATTAGG GAACGCTCGA ACTCGGCAAT GCCGGCGAAC ACGGTCAACA CCATGCGGCC GGCCGGCGTG GTGGTGTCGG CCCACGGCTC TGCCAGGCTA CGCAGGCCCG CGCCGGCCTC CTGGATGCGC TCGGCAATGT CCAGTAGGTC GCGGGTGCTG CGGGCCAGGC GGTCTAGCCT GGTCACTGTC ACAACGTCGC CAGGGCGTAG GTGGTCAAGC ATCCTGGCCA GCTCCGGGCG GTCGCGCCTG GTGCCGGTGA TCTTCTCGGA AAACAGCTTG GTGCAGCCGG CCGCGTGCAG TTCGGCCCGT TGGTTGGTCA AGTCCTGGTC GTCGGTGCTG ACGCGGGCAT AGCCCAGCAG GCCAGCGGCG GCGCTCTTGT TCATGGCGTA ATGTCTCCGG TTCTAGTCGC AAGTATTCTA CTTTATGCGA CTAAAACACG CGACAAGAAA ACGCCAGGAA AAGGGCAGGG CGGCAGCCTG TCGCGTAACT TAGGACTTGT GCGACATGTC GTTTTCAGAA GACGGCTGCA CTGAACGTCA GAAGCCGACT GCACTATAGC AGCGGAGGGG TTGGATCAAA GTACTTTGAT CCCGAGGGGA ACCCTGTGGT TGGCATGCAC ATACAAATGG ACGAACGGAT AAACCTTTTC ACGCCCTTTT AAATATCCGA TTATTCTAA
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