NM_013693.3;DIF; TNF-a; TNF-alpha; Tnfa; TNFalpha;Tnfsf1a; TNFSF2; Tnlg1f
100μg liquid plasmid(Instantly):$91
| name | pUC19-Fluc-Tnf(mouse) | another name | apUC19-Fluc-Tnf(mouse) |
| Insert Size (bp) | 708bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT ATGAAGCTTG CATGGTCGAC TCTAGAGGAT CCACCGGTCG CCACCATGGA AGACGCTAAA AACATCAAAA AAGGTCCGGC TCCGTTCTAC CCGCTGGAAG ACGGTACCGC TGGTGAACAG CTGCACAAAG CTATGAAACG TTACGCTCTG GTTCCGGGTA CCATCGCTTT CACCGACGCT CACATCGAAG TTGACATCAC CTACGCTGAA TACTTCGAAA TGTCTGTTCG TCTGGCTGAA GCTATGAAAC GTTACGGTCT GAACACCAAC CACCGTATCG TTGTTTGCTC TGAAAACTCT CTGCAGTTCT TCATGCCGGT TCTGGGTGCT CTGTTCATCG GTGTTGCTGT TGCTCCGGCT AACGACATCT ACAACGAACG TGAACTGCTG AACTCTATGG GTATCTCTCA GCCGACCGTT GTTTTCGTTT CTAAAAAAGG TCTGCAGAAA ATCCTGAACG TTCAGAAAAA ACTGCCGATC ATCCAGAAAA TCATCATCAT GGACTCTAAA ACCGACTACC AGGGTTTCCA GTCTATGTAC ACCTTCGTTA CCTCTCACCT GCCGCCGGGT TTCAACGAAT ACGACTTCGT TCCGGAATCT TTCGACCGTG ACAAAACCAT CGCTCTGATC ATGAACTCTT CTGGTTCTAC CGGTCTGCCG AAAGGTGTTG CTCTGCCGCA CCGTACCGCT TGCGTTCGTT TCTCTCACGC TCGTGACCCG ATCTTCGGTA ACCAGATCAT CCCGGACACC GCTATCCTGT CTGTTGTTCC GTTCCACCAC GGTTTCGGTA TGTTCACCAC CCTGGGTTAC CTGATCTGCG GTTTCCGTGT TGTTCTGATG TACCGTTTCG AAGAAGAACT GTTCCTGCGT TCTCTGCAGG ACTACAAAAT CCAGTCTGCT CTGCTGGTTC CGACCCTGTT CTCTTTCTTC GCTAAATCTA CCCTGATCGA CAAATACGAC CTGTCTAACC TGCACGAAAT CGCTTCTGGT GGTGCTCCGC TGTCTAAAGA AGTTGGTGAA GCTGTTGCTA AACGTTTCCA CCTGCCGGGT ATCCGTCAGG GTTACGGTCT GACCGAAACC ACCTCTGCTA TCCTGATCAC CCCGGAAGGT GACGACAAAC CGGGTGCTGT TGGTAAAGTT GTTCCGTTCT TCGAAGCTAA AGTTGTTGAC CTGGACACCG GTAAAACCCT GGGTGTTAAC CAGCGTGGTG AACTGTGCGT TCGTGGTCCG ATGATCATGT CTGGTTACGT TAACAACCCG GAAGCTACCA ACGCTCTGAT CGACAAAGAC GGTTGGCTGC ACTCTGGTGA CATCGCTTAC TGGGACGAAG ACGAACACTT CTTCATCGTT GACCGTCTGA AATCTCTGAT CAAATACAAA GGTTACCAGG TTGCTCCGGC TGAACTGGAA TCTATCCTGC TGCAGCACCC GAACATCTTC GACGCTGGTG TTGCTGGTCT GCCGGACGAC GACGCTGGTG AACTGCCGGC TGCTGTTGTT GTTCTGGAAC ACGGTAAAAC CATGACCGAA AAAGAAATCG TTGACTACGT TGCTTCTCAG GTTACCACCG CTAAAAAACT GCGTGGTGGT GTTGTTTTCG TTGACGAAGT TCCGAAAGGT CTGACCGGTA AACTGGACGC TCGTAAAATC CGTGAAATCC TGATCAAAGC TAAAAAAGGT GGTAAAATCG CTGTTGGTGG CGGTGGATCA GGCGGTGGAG GTTCTATGAG CACAGAAAGC ATGATCCGCG ACGTGGAACT GGCAGAAGAG GCACTCCCCC AAAAGATGGG GGGCTTCCAG AACTCCAGGC GGTGCCTATG TCTCAGCCTC TTCTCATTCC TGCTTGTGGC AGGGGCCACC ACGCTCTTCT GTCTACTGAA CTTCGGGGTG ATCGGTCCCC AAAGGGATGA GAAGTTCCCA AATGGCCTCC CTCTCATCAG TTCTATGGCC CAGACCCTCA CACTCAGATC ATCTTCTCAA AATTCGAGTG ACAAGCCTGT AGCCCACGTC GTAGCAAACC ACCAAGTGGA GGAGCAGCTG GAGTGGCTGA GCCAGCGCGC CAACGCCCTC CTGGCCAACG GCATGGATCT CAAAGACAAC CAACTAGTGG TGCCAGCCGA TGGGTTGTAC CTTGTCTACT CCCAGGTTCT CTTCAAGGGA CAAGGCTGCC CCGACTACGT GCTCCTCACC CACACCGTCA GCCGATTTGC TATCTCATAC CAGGAGAAAG TCAACCTCCT CTCTGCCGTC AAGAGCCCCT GCCCCAAGGA CACCCCTGAG GGGGCTGAGC TCAAACCCTG GTATGAGCCC ATATACCTGG GAGGAGTCTT CCAGCTGGAG AAGGGGGACC AACTCAGCGC TGAGGTCAAT CTGCCCAAGT ACTTAGACTT TGCGGAGTCC GGGCAGGTCT ACTTTGGAGT CATTGCTCTG TGATAATAGA ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG A
