pHDE-AtU626-lpa1-ipk-ipk1A(mays)-sgRNA-mCherry-UBQ-35S-Cas9
$84.00
In stock
SKU
PL40183
100μg liquid plasmid(Instantly):$127
| name | pHDE-AtU626-lpa1-ipk-ipk1A(mays)-sgRNA-mCherry-UBQ-35S-Cas9 | another name | apHDE-AtU626-lpa1-ipk-ipk1A(mays)-sgRNA-mCherry-UBQ-35S-Cas9 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Spe | |
| Selective marker | Hyg | Fluorescent labeling | Red |
| Promoter | 35S | Replicon | pVS1 |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTTACCCGC CAATATATCC TGTCAAACAC TGATAGTTTA AACTAGTAGT GTGCTGGAAT TGCCCTTAAG CTTCGTTGAA CAACGGAAAC TCGACTTGCC TTCCGCACAA TACATCATTT CTTCTTAGCT TTTTTTCTTC TTCTTCGTTC ATACAGTTTT TTTTTGTTTA TCAGCTTACA TTTTCTTGAA CCGTAGCTTT CGTTTTCTTC TTTTTAACTT TCCATTCGGA GTTTTTGTAT CTTGTTTCAT AGTTTGTCCC AGGATTAGAA TGATTAGGCA TCGAACCTTC AAGAATTTGA TTGAATAAAA CATCTTCATT CTTAAGATAT GAAGATAATC TTCAAAAGGC CCCTGGGAAT CTGAAAGAAG AGAAGCAGGC CCATTTATAT GGGAAAGAAC AATAGTATTT CTTATATAGG CCCATTTAAG TTGAAAACAA TCTTCAAAAG TCCCACATCG CTTAGATAAG AAAACGAAGC TGAGTTTATA TACAGCTAGA GTCGAAGTAG TGATTGGCTA CGGCGAAAAG AGTCTCGGTT TTAGAGCTAG AAATAGCAAG TTAAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC ACCGAGTCGG TGCTCTACTA TCGTCTATAC TCGGTTTTAG AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTTATC AACTTGAAAA AGTGGCACCG AGTCGGTGCC ATGTTATGAG CCAACACCTG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC GTTATCAACT TGAAAAAGTG GCACCGAGTC GGTGCTTTTT TTGTCCCTTC GAAGGGCAAT TCTGCAGATA TCCATCACAC TGGGAAGTGA AGCTTGGTCT CAACTATGAG ACCTAAGCTG GCACAACTAT ATTTCCAACA TCACTAGCTA CCATCAAAAG ATTGACTTCT CATCTTACTC GATTGAAACC AAATTAACAT AGGGTTTTTA TTTAAATAAA AGTTTAACCT TCTTTTTAAA AAATTGTTCA TAGTGTCATG TCAGAACAAG AGCTACAAAT CACACATAGC ATGCATAAGC GGAGCTATGA TGAGTGGTAT TGTTTTGTTC GTCACTTGTC ACTCTTTTCC AACACATAAT CCCGACAACA ACGTAAGAGC ATCTCTCTCT CTCCACACAC ACTCATGCAT GCATGCATTC TTACACGTGA TTGCCATGCA AATCTCCTTT CTCACCTATA AATACAAACC AACCCTTCAC TACACTCTTC ACTCAAACCA AAACAAGAAA ACATACACAA ATAGCAAAAC GGTACCAACA ATGGATAACA TGGCCATCAT CAAGGAGTTC ATGCGCTTCA AGGTGCACAT GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC GAGGGCGAGG GCCGCCCCTA CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG GGACATCCTG TCCCCTCAGT TCATGTACGG CTCCAAGGCC TACGTGAAGC ACCCCGCCGA CATCCCCGAC TACTTGAAGC TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC GCGTGATGAA CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC TCCCTGCAGG ACGGCGAGTT CATCTACAAG GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG TAATGCAGAA GAAGACCATG GGCTGGGAGG CCTCCTCCGA GCGGATGTAC CCCGAGGACG GCGCCCTGAA GGGCGAGATC AAGCAGAGGC TGAAGCTGAA GGACGGCGGC CACTACGACG CTGAGGTCAA GACCACCTAC AAGGCCAAGA AGCCCGTGCA GCTGCCCGGC GCCTACAACG TCAACATCAA GTTGGACATC ACCTCCCACA ACGAGGACTA CACCATCGTG GAACAGTACG AACGCGCCGA GGGCCGCCAC TCCACCGGCG GCATGGACGA GCTGTACAAG TGAGAGCTCT TGGACTCCCA TGTTGGCAAA GGCAACCAAA CAAACAATGA ATGATCCGCT CCTGCATATG GGGCGGTTTG AGTATTTCAA CTGCCATTTG GGCTGAATTG AAGACATGCT CCTGTCAGAA ATTCCGTGAT CTTACTCAAT ATTCAGTAAT CTCGGCCAAT ATCCTAAATG TGCGTGGCTT TATCTGTCTT TGTATTGTTT CATCAATTCA TGTAACGTTT GCTTTTCTTA TGAATTTTCA AATAAATTAT CGTATTGAGA CCGAATTCTC GTCGACGAGT CAGTAATAAA CGGCGTCAAA GTGGTTGCAG CCGGCACACA CGAGTCGTGT TTATCAACTC AAAGCACAAA TACTTTTCCT CAACCTAAAA ATAAGGCAAT TAGCCAAAAA CAACTTTGCG TGTAAACAAC GCTCAATACA CGTGTCATTT TATTATTAGC TATTGCTTCA CCGCCTTAGC TTTCTCGTGA CCTAGTCGTC CTCGTCTTTT CTTCTTCTTC TTCTATAAAA CAATACCCAA AGAGCTCTTC TTCTTCACAA TTCAGATTTC AATTTCTCAA AATCTTAAAA ACTTTCTCTC AATTCTCTCT ACCGTGATCA AGGTAAATTT CTGTGTTCCT TATTCTCTCA AAATCTTCGA TTTTGTTTTC GTTCGATCCC AATTTCGTAT ATGTTCTTTG GTTTAGATTC TGTTAATCTT AGATCGAAGA CGATTTTCTG GGTTTGATCG TTAGATATCA TCTTAATTCT CGATTAGGGT TTCATAGATA TCATCCGATT TGTTCAAATA ATTTGAGTTT TGTCGAATAA TTACTCTTCG ATTTGTGATT TCTATCTAGA TCTGGTGTTA GTTTCTAGTT TGTGCGATCG AATTTGTCGA TTAATCTGAG TTTTTCTGAT TAACAGCTCG CAATTGACCT AACTGAGTAA GCTAGCTTGT TCGAGTATTA TGGCATTGGG AAAACTGTTT TTCTTGTACC ATTTGTTGTG CTTGTAATTT ACTGTGTTTT TTATTCGGTT TTCGCTATCG AACTGTGAAA TGGAAATGGA TGGAGAAGAG TTAATGAATG ATATGGTCCT TTTGTTCATT CTCAAATTAA TATTATTTGT TTTTTCTCTT ATTTGTTGTG TGTTGAATTT GAAATTATAA GAGATATGCA AACATTTTGT TTTGAGTAAA AATGTGTCAA ATCGTGGCCT CTAATGACCG AAGTTAATAT GAGGAGTAAA ACACTAGATC CCCAAACAAG CTTGAAACTG AAGGCGGGAA ACGACAATCT GATCATGAGC GGAGAATTAA GGGAGTCACG TTATGACCCC CGCCGATGAC GCGGGACAAG CCGTTTTACG TTTGGAACTG ACAGAACCGC AACGTTGAAG GAGCCACTCA GCCGCGGGTT TCTGGAGTTT AATGAGCTAA GCACATACGT CAGAAACCAT TATTGCGCGT TCAAAAGTCG CCTAAGGTCA CTATCAGCTA GCAAATATTT CTTGTCAAAA ATGCTCCACT GACGTTCCAT AAATTCCCCT CGGTATCCAA TTAGAGTCTC ATATTCACTC TCAATCCAAA TAATCTGCAC CGGATCCGCT AGAGGATCTC GACCTGCAAG ATCCCGGGGG GCAATGAGAT ATGAAAAAGC CTGAACTCAC CGCGACGTCT GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT ATAGGAGGGC GTGGATATGT CCTGCGGGTA AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GGGATCTTGG AGGTGATGTA ACATGATCAC AAGCTGATCC CCCGAATTTC CCCGATCGTT CAAACATTTG GCAATAAAGT TTCTTAAGAT TGAATCCTGT TGCCGGTCTT GCGATGATTA TCATATAATT TCTGTTGAAT TACGTTAAGC ATGTAATAAT TAACATGTAA TGCATGACGT TATTTATGAG ATGGGTTTTT ATGATTAGAG TCCCGCAATT ATACATTTAA TACGCGATAG AAAACAAAAT ATAGCGCGCA AACTAGGATA AATTATCGCG CGCGGTGTCA TCTATGTTAC TAGATCGGGA ATTGATCCCC CCTCGACAGC TTGCATGCCA GCTTGGGCTG CAGGTCGACG CGTCAACATG GTGGAGCACG ACACTCTCGT CTACTCCAAG AATATCAAAG ATACAGTCTC AGAAGACCAA AGGGCTATTG AGACTTTTCA ACAAAGGGTA ATATCGGGAA ACCTCCTCGG ATTCCATTGC CCAGCTATCT GTCACTTCAT CAAAAGGACA GTAGAAAAGG AAGGTGGCAC CTACAAATGC CATCATTGCG ATAAAGGAAA GGCTATCGTT CAAGATGCCT CTGCCGACAG TGGTCCCAAA GATGGACCCC CACCCACGAG GAGCATCGTG GAAAAAGAAG ACGTTCCAAC CACGTCTTCA AAGCAAGTGG ATTGATGTGA TATCTCCACT GACGTAAGGG ATGACGCACA ATCCCACTAT CCTTCGCAAG ACCCTTCCTC TATATAAGGA AGTTCATTTC ATTTGGAGAG GACACGATAT GGACAAGAAG TACTCCATTG GGCTCGATAT CGGCACAAAC AGCGTCGGCT GGGCCGTCAT TACGGACGAG TACAAGGTGC CGAGCAAAAA ATTCAAAGTT CTGGGCAATA CCGATCGCCA CAGCATAAAG AAGAACCTCA TTGGCGCCCT CCTGTTCGAC TCCGGGGAGA CGGCCGAAGC CACGCGGCTC AAAAGAACAG CACGGCGCAG ATATACCCGC AGAAAGAATC GGATCTGCTA CCTGCAGGAG ATCTTTAGTA ATGAGATGGC TAAGGTGGAT GACTCTTTCT TCCATAGGCT GGAGGAGTCC TTTTTGGTGG AGGAGGATAA AAAGCACGAG CGCCACCCAA TCTTTGGCAA TATCGTGGAC GAGGTGGCGT ACCATGAAAA GTACCCAACC ATATATCATC TGAGGAAGAA GCTTGTAGAC AGTACTGATA AGGCTGACTT GCGGTTGATC TATCTCGCGC TGGCGCATAT GATCAAATTT CGGGGACACT TCCTCATCGA GGGGGACCTG AACCCAGACA ACAGCGATGT CGACAAACTC TTTATCCAAC TGGTTCAGAC TTACAATCAG CTTTTCGAAG AGAACCCGAT CAACGCATCC GGAGTTGACG CCAAAGCAAT CCTGAGCGCT AGGCTGTCCA AATCCCGGCG GCTCGAAAAC CTCATCGCAC AGCTCCCTGG GGAGAAGAAG AACGGCCTGT TTGGTAATCT TATCGCCCTG TCACTCGGGC TGACCCCCAA CTTTAAATCT AACTTCGACC TGGCCGAAGA TGCCAAGCTT CAACTGAGCA AAGACACCTA CGATGATGAT CTCGACAATC TGCTGGCCCA GATCGGCGAC CAGTACGCAG ACCTTTTTTT GGCGGCAAAG AACCTGTCAG ACGCCATTCT GCTGAGTGAT ATTCTGCGAG TGAACACGGA GATCACCAAA GCTCCGCTGA GCGCTAGTAT GATCAAGCGC TATGATGAGC ACCACCAAGA CTTGACTTTG CTGAAGGCCC TTGTCAGACA GCAACTGCCT GAGAAGTACA AGGAAATTTT CTTCGATCAG TCTAAAAATG GCTACGCCGG ATACATTGAC GGCGGAGCAA GCCAGGAGGA ATTTTACAAA TTTATTAAGC CCATCTTGGA AAAAATGGAC GGCACCGAGG AGCTGCTGGT AAAGCTTAAC AGAGAAGATC TGTTGCGCAA ACAGCGCACT TTCGACAATG GAAGCATCCC CCACCAGATT CACCTGGGCG AACTGCACGC TATCCTCAGG CGGCAAGAGG ATTTCTACCC CTTTTTGAAA GATAACAGGG AAAAGATTGA GAAAATCCTC ACATTTCGGA TACCCTACTA TGTAGGCCCC CTCGCCCGGG GAAATTCCAG ATTCGCGTGG ATGACTCGCA AATCAGAAGA GACCATCACT CCCTGGAACT TCGAGGAAGT CGTGGATAAG GGGGCCTCTG CCCAGTCCTT CATCGAAAGG ATGACTAACT TTGATAAAAA TCTGCCTAAC GAAAAGGTGC TTCCTAAACA CTCTCTGCTG TACGAGTACT TCACAGTTTA TAACGAGCTC ACCAAGGTCA AATACGTCAC AGAAGGGATG AGAAAGCCAG CATTCCTGTC TGGAGAGCAG AAGAAAGCTA TCGTGGACCT CCTCTTCAAG ACGAACCGGA AAGTTACCGT GAAACAGCTC AAAGAAGACT ATTTCAAAAA GATTGAATGT TTCGACTCTG TTGAAATCAG CGGAGTGGAG GATCGCTTCA ACGCATCCCT GGGAACGTAT CACGATCTCC TGAAAATCAT TAAAGACAAG GACTTCCTGG ACAATGAGGA GAACGAGGAC ATTCTTGAGG ACATTGTCCT CACCCTTACG TTGTTTGAAG ATAGGGAGAT GATTGAAGAA CGCTTGAAAA CTTACGCTCA TCTCTTCGAC GACAAAGTCA TGAAACAGCT CAAGAGGCGC CGATATACAG GATGGGGGCG GCTGTCAAGA AAACTGATCA ATGGGATCCG AGACAAGCAG AGTGGAAAGA CAATCCTGGA TTTTCTTAAG TCCGATGGAT TTGCCAACCG GAACTTCATG CAGTTGATCC ATGATGACTC TCTCACCTTT AAGGAGGACA TCCAGAAAGC ACAAGTTTCT GGCCAGGGGG ACAGTCTTCA CGAGCACATC GCTAATCTTG CAGGTAGCCC AGCTATCAAA AAGGGAATAC TGCAGACCGT TAAGGTCGTG GATGAACTCG TCAAAGTAAT GGGAAGGCAT AAGCCCGAGA ATATCGTTAT CGAGATGGCC CGAGAGAACC AAACTACCCA GAAGGGACAG AAGAACAGTA GGGAAAGGAT GAAGAGGATT GAAGAGGGTA TAAAAGAACT GGGGTCCCAA ATCCTTAAGG AACACCCAGT TGAAAACACC CAGCTTCAGA ATGAGAAGCT CTACCTGTAC TACCTGCAGA ACGGCAGGGA CATGTACGTG GATCAGGAAC TGGACATCAA TCGGCTCTCC GACTACGACG TGGATCATAT CGTGCCCCAG TCTTTTCTCA AAGATGATTC TATTGATAAT AAAGTGTTGA CAAGATCCGA TAAAAATAGA GGGAAGAGTG ATAACGTCCC CTCAGAAGAA GTTGTCAAGA AAATGAAAAA TTATTGGCGG CAGCTGCTGA ACGCCAAACT GATCACACAA CGGAAGTTCG ATAATCTGAC TAAGGCTGAA CGAGGTGGCC TGTCTGAGTT GGATAAAGCC GGCTTCATCA AAAGGCAGCT TGTTGAGACA CGCCAGATCA CCAAGCACGT GGCCCAAATT CTCGATTCAC GCATGAACAC CAAGTACGAT GAAAATGACA AACTGATTCG AGAGGTGAAA GTTATTACTC TGAAGTCTAA GCTGGTCTCA GATTTCAGAA AGGACTTTCA GTTTTATAAG GTGAGAGAGA TCAACAATTA CCACCATGCG CATGATGCCT ACCTGAATGC AGTGGTAGGC ACTGCACTTA TCAAAAAATA TCCCAAGCTT GAATCTGAAT TTGTTTACGG AGACTATAAA GTGTACGATG TTAGGAAAAT GATCGCAAAG TCTGAGCAGG AAATAGGCAA GGCCACCGCT AAGTACTTCT TTTACAGCAA TATTATGAAT TTTTTCAAGA CCGAGATTAC ACTGGCCAAT GGAGAGATTC GGAAGCGACC ACTTATCGAA ACAAACGGAG AAACAGGAGA AATCGTGTGG GACAAGGGTA GGGATTTCGC GACAGTCCGG AAGGTCCTGT CCATGCCGCA GGTGAACATC GTTAAAAAGA CCGAAGTACA GACCGGAGGC TTCTCCAAGG AAAGTATCCT CCCGAAAAGG AACAGCGACA AGCTGATCGC ACGCAAAAAA GATTGGGACC CCAAGAAATA CGGCGGATTC GATTCTCCTA CAGTCGCTTA CAGTGTACTG GTTGTGGCCA AAGTGGAGAA AGGGAAGTCT AAAAAACTCA AAAGCGTCAA GGAACTGCTG GGCATCACAA TCATGGAGCG ATCAAGCTTC GAAAAAAACC CCATCGACTT TCTCGAGGCG AAAGGATATA AAGAGGTCAA AAAAGACCTC ATCATTAAGC TTCCCAAGTA CTCTCTCTTT GAGCTTGAAA ACGGCCGGAA ACGAATGCTC GCTAGTGCGG GCGAGCTGCA GAAAGGTAAC GAGCTGGCAC TGCCCTCTAA ATACGTTAAT TTCTTGTATC TGGCCAGCCA CTATGAAAAG CTCAAAGGGT CTCCCGAAGA TAATGAGCAG AAGCAGCTGT TCGTGGAACA ACACAAACAC TACCTTGATG AGATCATCGA GCAAATAAGC GAATTCTCCA AAAGAGTGAT CCTCGCCGAC GCTAACCTCG ATAAGGTGCT TTCTGCTTAC AATAAGCACA GGGATAAGCC CATCAGGGAG CAGGCAGAAA ACATTATCCA CTTGTTTACT CTGACCAACT TGGGCGCGCC TGCAGCCTTC AAGTACTTCG ACACCACCAT AGACAGAAAG CGGTACACCT CTACAAAGGA GGTCCTGGAC GCCACACTGA TTCATCAGTC AATTACGGGG CTCTATGAAA CAAGAATCGA CCTCTCTCAG CTCGGTGGAG ACAGCAGGGC TGACCCCAAG AAGAAGAGGA AGGTGTGATC GATCCAGGCC TCCCAGCTTT CGTCCGTATC ATCGGTTTCG ACAACGTTCG TCAAGTTCAA TGCATCAGTT TCATTGCCCA CACACCAGAA TCCTACTAAG TTTGAGTATT ATGGCATTGG AAAAGCTGTT TTCTTCTATC ATTTGTTCTG CTTGTAATTT ACTGTGTTCT TTCAGTTTTT GTTTTCGGAC ATCAAAATGC AAATGGATGG ATAAGAGTTA ATAAATGATA TGGTCCTTTT GTTCATTCTC AAATTATTAT TATCTGTTGT TTTTACTTTA ATGGGTTGAA TTTAAGTAAG AAAGGAACTA ACAGTGTGAT ATTAAGGTGC AATGTTAGAC ATATAAAACA GTCTTTCACC TCTCTTTGGT TATGTCTTGA ATTGGTTTGT TTCTTCACTT ATCTGTGTAA TCAAGTTTAC TATGAGTCTA TGATCAAGTA ATTATGCAAT CAAGTTAAGT ACAGTATAGG CTTTTTGTGT CGAGGGGGTA CCGAGTCGAG GAATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGGG TACCGAGCTC GAATTCAATT CGGCGTTAAT TCAGTACATT AAAAACGTCC GCAATGTGTT ATTAAGTTGT CTAAGCGTCA ATTTGTTTAC ACCACAATAT ATCCTGCCAC CAGCCAGCCA ACAGCTCCCC GACCGGCAGC TCGGCACAAA ATCACCACTC GATACAGGCA GCCCATCAGT CCGGGACGGC GTCAGCGGGA GAGCCGTTGT AAGGCGGCAG ACTTTGCTCA TGTTACCGAT GCTATTCGGA AGAACGGCAA CTAAGCTGCC GGGTTTGAAA CACGGATGAT CTCGCGGAGG GTAGCATGTT GATTGTAACG ATGACAGAGC GTTGCTGCCT GTGATCAATT CGGGCACGAA CCCAGTGGAC ATAAGCCTCG TTCGGTTCGT AAGCTGTAAT GCAAGTAGCG TAACTGCCGT CACGCAACTG GTCCAGAACC TTGACCGAAC GCAGCGGTGG TAACGGCGCA GTGGCGGTTT TCATGGCTTC TTGTTATGAC ATGTTTTTTT GGGGTACAGT CTATGCCTCG GGCATCCAAG CAGCAAGCGC GTTACGCCGT GGGTCGATGT TTGATGTTAT GGAGCAGCAA CGATGTTACG CAGCAGGGCA GTCGCCCTAA AACAAAGTTA AACATCATGG GGGAAGCGGT GATCGCCGAA GTATCGACTC AACTATCAGA GGTAGTTGGC GTCATCGAGC GCCATCTCGA ACCGACGTTG CTGGCCGTAC ATTTGTACGG CTCCGCAGTG GATGGCGGCC TGAAGCCACA CAGTGATATT GATTTGCTGG TTACGGTGAC CGTAAGGCTT GATGAAACAA CGCGGCGAGC TTTGATCAAC GACCTTTTGG AAACTTCGGC TTCCCCTGGA GAGAGCGAGA TTCTCCGCGC TGTAGAAGTC ACCATTGTTG TGCACGACGA CATCATTCCG TGGCGTTATC CAGCTAAGCG CGAACTGCAA TTTGGAGAAT GGCAGCGCAA TGACATTCTT GCAGGTATCT TCGAGCCAGC CACGATCGAC ATTGATCTGG CTATCTTGCT GACAAAAGCA AGAGAACATA GCGTTGCCTT GGTAGGTCCA GCGGCGGAGG AACTCTTTGA TCCGGTTCCT GAACAGGATC TATTTGAGGC GCTAAATGAA ACCTTAACGC TATGGAACTC GCCGCCCGAC TGGGCTGGCG ATGAGCGAAA TGTAGTGCTT ACGTTGTCCC GCATTTGGTA CAGCGCAGTA ACCGGCAAAA TCGCGCCGAA GGATGTCGCT GCCGACTGGG CAATGGAGCG CCTGCCGGCC CAGTATCAGC CCGTCATACT TGAAGCTAGA CAGGCTTATC TTGGACAAGA AGAAGATCGC TTGGCCTCGC GCGCAGATCA GTTGGAAGAA TTTGTCCACT ACGTGAAAGG CGAGATCACC AAGGTAGTCG GCAAATAATG TCTAGCTAGA AATTCGTTCA AGCCGACGCC GCTTCGCCGG CGTTAACTCA AGCGATTAGA TGCACTAAGC ACATAATTGC TCACAGCCAA ACTATCAGGT CAAGTCTGCT TTTATTATTT TTAAGCGTGC ATAATAAGCC CTACACAAAT TGGGAGATAT ATCATGCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGGGT GCCTTGATGT GGGCGCCGGC GGTCGAGTGG CGACGGCGCG GCTTGTCCGC GCCCTGGTAG ATTGCCTGGC CGTAGGCCAG CCATTTTTGA GCGGCCAGCG GCCGCGATAG GCCGACGCGA AGCGGCGGGG CGTAGGGAGC GCAGCGACCG AAGGGTAGGC GCTTTTTGCA GCTCTTCGGC TGTGCGCTGG CCAGACAGTT ATGCACAGGC CAGGCGGGTT TTAAGAGTTT TAATAAGTTT TAAAGAGTTT TAGGCGGAAA AATCGCCTTT TTTCTCTTTT ATATCAGTCA CTTACATGTG TGACCGGTTC CCAATGTACG GCTTTGGGTT CCCAATGTAC GGGTTCCGGT TCCCAATGTA CGGCTTTGGG TTCCCAATGT ACGTGCTATC CACAGGAAAG AGACCTTTTC GACCTTTTTC CCCTGCTAGG GCAATTTGCC CTAGCATCTG CTCCGTACAT TAGGAACCGG CGGATGCTTC GCCCTCGATC AGGTTGCGGT AGCGCATGAC TAGGATCGGG CCAGCCTGCC CCGCCTCCTC CTTCAAATCG TACTCCGGCA GGTCATTTGA CCCGATCAGC TTGCGCACGG TGAAACAGAA CTTCTTGAAC TCTCCGGCGC TGCCACTGCG TTCGTAGATC GTCTTGAACA ACCATCTGGC TTCTGCCTTG CCTGCGGCGC GGCGTGCCAG GCGGTAGAGA AAACGGCCGA TGCCGGGATC GATCAAAAAG TAATCGGGGT GAACCGTCAG CACGTCCGGG TTCTTGCCTT CTGTGATCTC GCGGTACATC CAATCAGCTA GCTCGATCTC GATGTACTCC GGCCGCCCGG TTTCGCTCTT TACGATCTTG TAGCGGCTAA TCAAGGCTTC ACCCTCGGAT ACCGTCACCA GGCGGCCGTT CTTGGCCTTC TTCGTACGCT GCATGGCAAC GTGCGTGGTG TTTAACCGAA TGCAGGTTTC TACCAGGTCG TCTTTCTGCT TTCCGCCATC GGCTCGCCGG CAGAACTTGA GTACGTCCGC AACGTGTGGA CGGAACACGC GGCCGGGCTT GTCTCCCTTC CCTTCCCGGT ATCGGTTCAT GGATTCGGTT AGATGGGAAA CCGCCATCAG TACCAGGTCG TAATCCCACA CACTGGCCAT GCCGGCCGGC CCTGCGGAAA CCTCTACGTG CCCGTCTGGA AGCTCGTAGC GGATCACCTC GCCAGCTCGT CGGTCACGCT TCGACAGACG GAAAACGGCC ACGTCCATGA TGCTGCGACT ATCGCGGGTG CCCACGTCAT AGAGCATCGG AACGAAAAAA TCTGGTTGCT CGTCGCCCTT GGGCGGCTTC CTAATCGACG GCGCACCGGC TGCCGGCGGT TGCCGGGATT CTTTGCGGAT TCGATCAGCG GCCGCTTGCC ACGATTCACC GGGGCGTGCT TCTGCCTCGA TGCGTTGCCG CTGGGCGGCC TGCGCGGCCT TCAACTTCTC CACCAGGTCA TCACCCAGCG CCGCGCCGAT TTGTACCGGG CCGGATGGTT TGCGACCGTC ACGCCGATTC CTCGGGCTTG GGGGTTCCAG TGCCATTGCA GGGCCGGCAG ACAACCCAGC CGCTTACGCC TGGCCAACCG CCCGTTCCTC CACACATGGG GCATTCCACG GCGTCGGTGC CTGGTTGTTC TTGATTTTCC ATGCCGCCTC CTTTAGCCGC TAAAATTCAT CTACTCATTT ATTCATTTGC TCATTTACTC TGGTAGCTGC GCGATGTATT CAGATAGCAG CTCGGTAATG GTCTTGCCTT GGCGTACCGC GTACATCTTC AGCTTGGTGT GATCCTCCGC CGGCAACTGA AAGTTGACCC GCTTCATGGC TGGCGTGTCT GCCAGGCTGG CCAACGTTGC AGCCTTGCTG CTGCGTGCGC TCGGACGGCC GGCACTTAGC GTGTTTGTGC TTTTGCTCAT TTTCTCTTTA CCTCATTAAC TCAAATGAGT TTTGATTTAA TTTCAGCGGC CAGCGCCTGG ACCTCGCGGG CAGCGTCGCC CTCGGGTTCT GATTCAAGAA CGGTTGTGCC GGCGGCGGCA GTGCCTGGGT AGCTCACGCG CTGCGTGATA CGGGACTCAA GAATGGGCAG CTCGTACCCG GCCAGCGCCT CGGCAACCTC ACCGCCGATG CGCGTGCCTT TGATCGCCCG CGACACGACA AAGGCCGCTT GTAGCCTTCC ATCCGTGACC TCAATGCGCT GCTTAACCAG CTCCACCAGG TCGGCGGTGG CCCATATGTC GTAAGGGCTT GGCTGCACCG GAATCAGCAC GAAGTCGGCT GCCTTGATCG CGGACACAGC CAAGTCCGCC GCCTGGGGCG CTCCGTCGAT CACTACGAAG TCGCGCCGGC CGATGGCCTT CACGTCGCGG TCAATCGTCG GGCGGTCGAT GCCGACAACG GTTAGCGGTT GATCTTCCCG CACGGCCGCC CAATCGCGGG CACTGCCCTG GGGATCGGAA TCGACTAACA GAACATCGGC CCCGGCGAGT TGCAGGGCGC GGGCTAGATG GGTTGCGATG GTCGTCTTGC CTGACCCGCC TTTCTGGTTA AGTACAGCGA TAACCTTCAT GCGTTCCCCT TGCGTATTTG TTTATTTACT CATCGCATCA TATACGCAGC GACCGCATGA CGCAAGCTGT TTTACTCAAA TACACATCAC CTTTTTAGAC GGCGGCGCTC GGTTTCTTCA GCGGCCAAGC TGGCCGGCCA GGCCGCCAGC TTGGCATCAG ACAAACCGGC CAGGATTTCA TGCAGCCGCA CGGTTGAGAC GTGCGCGGGC GGCTCGAACA CGTACCCGGC CGCGATCATC TCCGCCTCGA TCTCTTCGGT AATGAAAAAC GGTTCGTCCT GGCCGTCCTG GTGCGGTTTC ATGCTTGTTC CTCTTGGCGT TCATTCTCGG CGGCCGCCAG GGCGTCGGCC TCGGTCAATG CGTCCTCACG GAAGGCACCG CGCCGCCTGG CCTCGGTGGG CGTCACTTCC TCGCTGCGCT CAAGTGCGCG GTACAGGGTC GAGCGATGCA CGCCAAGCAG TGCAGCCGCC TCTTTCACGG TGCGGCCTTC CTGGTCGATC AGCTCGCGGG CGTGCGCGAT CTGTGCCGGG GTGAGGGTAG GGCGGGGGCC AAACTTCACG CCTCGGGCCT TGGCGGCCTC GCGCCCGCTC CGGGTGCGGT CGATGATTAG GGAACGCTCG AACTCGGCAA TGCCGGCGAA CACGGTCAAC ACCATGCGGC CGGCCGGCGT GGTGGTGTCG GCCCACGGCT CTGCCAGGCT ACGCAGGCCC GCGCCGGCCT CCTGGATGCG CTCGGCAATG TCCAGTAGGT CGCGGGTGCT GCGGGCCAGG CGGTCTAGCC TGGTCACTGT CACAACGTCG CCAGGGCGTA GGTGGTCAAG CATCCTGGCC AGCTCCGGGC GGTCGCGCCT GGTGCCGGTG ATCTTCTCGG AAAACAGCTT GGTGCAGCCG GCCGCGTGCA GTTCGGCCCG TTGGTTGGTC AAGTCCTGGT CGTCGGTGCT GACGCGGGCA TAGCCCAGCA GGCCAGCGGC GGCGCTCTTG TTCATGGCGT AATGTCTCCG GTTCTAGTCG CAAGTATTCT ACTTTATGCG ACTAAAACAC GCGACAAGAA AACGCCAGGA AAAGGGCAGG GCGGCAGCCT GTCGCGTAAC TTAGGACTTG TGCGACATGT CGTTTTCAGA AGACGGCTGC ACTGAACGTC AGAAGCCGAC TGCACTATAG CAGCGGAGGG GTTGGATCAA AGTACTTTAA AGTACTTTAA AGTACTTTAA AGTACTTTGA TCCCGAGGGG AACCCTGTGG TTGGCATGCA CATACAAATG GACGAACGGA TAAACCTTTT CACGCCCTTT TAAATATCCG ATTATTCTAA TAAACGCTCT TTTCTCTTAG
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