NM_014495.4;ANG-5; ANGPT5; ANL3; FHBL2
100μg liquid plasmid(Instantly):$91
| name | pLV2-CMV-Rluc-5UTR-ANGPTL3(human)-3UTR-Puro | another name | apLV2-CMV-Rluc-5UTR-ANGPTL3(human)-3UTR-Puro |
| Insert Size (bp) | 1383bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCGAA TTCACAAATG GCAGTATTCA TCCACAATTT TAAAAGAAAA GGGGGGATTG GGGGGTACAG TGCAGGGGAA AGAATAGTAG ACATAATAGC AACAGACATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA ATTTTCGGGT TTATTACAGG GACAGCAGAG ATCCAGTTTG GACTAGTGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GGCGGCCACG CGTGCCACCA TGGCTTCCAA GGTGTACGAC CCCGAGCAAC GCAAACGCAT GATCACTGGG CCTCAGTGGT GGGCTCGCTG CAAGCAAATG AACGTGCTGG ACTCCTTCAT CAACTACTAT GATTCCGAGA AGCACGCCGA GAACGCCGTG ATTTTTCTGC ATGGTAACGC TGCCTCCAGC TACCTGTGGA GGCACGTCGT GCCTCACATC GAGCCCGTGG CTAGATGCAT CATCCCTGAT CTGATCGGAA TGGGTAAGTC CGGCAAGAGC GGGAATGGCT CATATCGCCT CCTGGATCAC TACAAGTACC TCACCGCTTG GTTCGAGCTG CTGAACCTTC CAAAGAAAAT CATCTTTGTG GGCCACGACT GGGGGGCTTG TCTGGCCTTT CACTACTCCT ACGAGCACCA AGACAAGATC AAGGCCATCG TCCATGCTGA GAGTGTCGTG GACGTGATCG AGTCCTGGGA CGAGTGGCCT GACATCGAGG AGGATATCGC CCTGATCAAG AGCGAAGAGG GCGAGAAAAT GGTGCTTGAG AATAACTTCT TCGTCGAGAC CATGCTCCCA AGCAAGATCA TGCGGAAACT GGAGCCTGAG GAGTTCGCTG CCTACCTGGA GCCATTCAAG GAGAAGGGCG AGGTTAGACG GCCTACCCTC TCCTGGCCTC GCGAGATCCC TCTCGTTAAG GGAGGCAAGC CCGACGTCGT CCAGATTGTC CGCAACTACA ACGCCTACCT TCGGGCCAGC GACGATCTGC CTAAGATGTT CATCGAGTCC GACCCTGGGT TCTTTTCCAA CGCTATTGTC GAGGGAGCTA AGAAGTTCCC TAACACCGAG TTCGTGAAGG TGAAGGGCCT CCACTTCAGC CAGGAGGACG CTCCAGATGA AATGGGTAAG TACATCAAGA GCTTCGTGGA GCGCGTGCTG AAGAACGAGC AGTAATTCTA GGCGATCAGA AGAAAACAGT TCCACGTTGC TTGAAATTGA AAATCAAGAT AAAAATGTTC ACAATTAAGC TCCTTCTTTT TATTGTTCCT CTAGTTATTT CCTCCAGAAT TGATCAAGAC AATTCATCAT TTGATTCTCT ATCTCCAGAG CCAAAATCAA GATTTGCTAT GTTAGACGAT GTAAAAATTT TAGCCAATGG CCTCCTTCAG TTGGGACATG GTCTTAAAGA CTTTGTCCAT AAGACGAAGG GCCAAATTAA TGACATATTT CAAAAACTCA ACATATTTGA TCAGTCTTTT TATGATCTAT CGCTGCAAAC CAGTGAAATC AAAGAAGAAG AAAAGGAACT GAGAAGAACT ACATATAAAC TACAAGTCAA AAATGAAGAG GTAAAGAATA TGTCACTTGA ACTCAACTCA AAACTTGAAA GCCTCCTAGA AGAAAAAATT CTACTTCAAC AAAAAGTGAA ATATTTAGAA GAGCAACTAA CTAACTTAAT TCAAAATCAA CCTGAAACTC CAGAACACCC AGAAGTAACT TCACTTAAAA CTTTTGTAGA AAAACAAGAT AATAGCATCA AAGACCTTCT CCAGACCGTG GAAGACCAAT ATAAACAATT AAACCAACAG CATAGTCAAA TAAAAGAAAT AGAAAATCAG CTCAGAAGGA CTAGTATTCA AGAACCCACA GAAATTTCTC TATCTTCCAA GCCAAGAGCA CCAAGAACTA CTCCCTTTCT TCAGTTGAAT GAAATAAGAA ATGTAAAACA TGATGGCATT CCTGCTGAAT GTACCACCAT TTATAACAGA GGTGAACATA CAAGTGGCAT GTATGCCATC AGACCCAGCA ACTCTCAAGT TTTTCATGTC TACTGTGATG TTATATCAGG TAGTCCATGG ACATTAATTC AACATCGAAT AGATGGATCA CAAAACTTCA ATGAAACGTG GGAGAACTAC AAATATGGTT TTGGGAGGCT TGATGGAGAA TTTTGGTTGG GCCTAGAGAA GATATACTCC ATAGTGAAGC AATCTAATTA TGTTTTACGA ATTGAGTTGG AAGACTGGAA AGACAACAAA CATTATATTG AATATTCTTT TTACTTGGGA AATCACGAAA CCAACTATAC GCTACATCTA GTTGCGATTA CTGGCAATGT CCCCAATGCA ATCCCGGAAA ACAAAGATTT GGTGTTTTCT ACTTGGGATC ACAAAGCAAA AGGACACTTC AACTGTCCAG AGGGTTATTC AGGAGGCTGG TGGTGGCATG ATGAGTGTGG AGAAAACAAC CTAAATGGTA AATATAACAA ACCAAGAGCA AAATCTAAGC CAGAGAGGAG AAGAGGATTA TCTTGGAAGT CTCAAAATGG AAGGTTATAC TCTATAAAAT CAACCAAAAT GTTGATCCAT CCAACAGATT CAGAAAGCTT TGAATGAACT GAGGCAAATT TAAAAGGCAA TAATTTAAAC ATTAACCTCA TTCCAAGTTA ATGTGGTCTA ATAATCTGGT ATTAAATCCT TAAGAGAAAG CTTGAGAAAT AGATTTTTTT TATCTTAAAG TCACTGTCTA TTTAAGATTA AACATACAAT CACATAACCT TAAAGAATAC CGTTTACATT TCTCAATCAA AATTCTTATA ATACTATTTG TTTTAAATTT TGTGATGTGG GAATCAATTT TAGATGGTCA CAATCTAGAT TATAATCAAT AGGTGAACTT ATTAAATAAC TTTTCTAAAT AAAAAATTTA GAGACTTTTA TTTTAAAAGG CATCATATGA GCTAATATCA CAACTTTCCC AGTTTAAAAA ACTAGTACTC TTGTTAAAAC TCTAAACTTG ACTAAATACA GAGGACTGGT AATTGTACAG TTCTTAAATG TTGTAGTATT AATTTCAAAA CTAAAAATCG TCAGCACAGA GTATGTGTAA AAATCTGTAA TACAAATTTT TAAACTGATG CTTCATTTTG CTACAAAATA ATTTGGAGTA AATGTTTGAT ATGATTTATT TATGAAACCT AATGAAGCAG AATTAAATAC TGTATTAAAA TAAGTTCGCT GTCTTTAAAC AAATGGAGAT GACTACTAAG TCACATTGAC TTTAACATGA GGTATCACTA TACCTTATTT GTTAAAATAT ATACTGTATA CATTTTATAT ATTTTAACAC TTAATACTAT GAAAACAAAT AATTGTAAAG GAATCTTGTC AGATTACAGT AAGAATGAAC ATATTTGTGG CATCGAGTTA AAGTTTATAT TTCCCCTAAA TATGCTGTGA TTCTAATACA TTCGTGTAGG TTTTCAAGTA GAAATAAACC TCGTAACAAG TTACTGAACG TTTAAACAGC CTGACAAGCA TGTATATATG TTTAAAATTC AATAAACAAA GACCCAGTCC CTAAATTATA GAAATTTAAA TTATTCTTGC ATGTTTATCG ACATCACAAC AGATCCCTAA ATCCCTAAAT CCCTAAAGAT TAGATACAAA TTTTTTACCA CAGTATCACT TGTCAGAATT TATTTTTAAA TATGATTTTT TAAAACTGCC AGTAAGAAAT TTTAAATTAA ACCCATTTGT TAAAGGATAT AGTGCCCAAG TTATATGGTG ACCTACCTTT GTCAATACTT AGCATTATGT ATTTCAAATT ATCCAATATA CATGTCATAT ATATTTTTAT ATGTCACATA TATAAAAGAT ATGTATGATC TATGTGAATC CTAAGTAAAT ATTTTGTTCC AGAAAAGTAC AAAATAATAA AGGTAAAAAT AATCTATAAT TTTCAGGACC ACAGACTAAG CTGTCGAAAT TAACGCTGAT TTTTTTAGGG CCAGAATACC AAAATGGCTC CTCTCTTCCC CCAAAATTGG ACAATTTCAA ATGCAAAATA ATTCATTATT TAATATATGA GTTGCTTCCT CTATTTGGTT TCCGCGGCCG CTGGCCGCAA TAAAATATCT TTATTTTCAT TACATCTGTG TGTTGGTTTT TTGTGTGAGG ATTCGAGAAT TCTACCGGGT AGGGGAGGCG CTTTTCCCAA GGCAGTCTGG AGCATGCGCT TTAGCAGCCC CGCTGGGCAC TTGGCGCTAC ACAAGTGGCC TCTGGCCTCG CACACATTCC ACATCCACCG GTAGGCGCCA ACCGGCTCCG TTCTTTGGTG GCCCCTTCGC GCCACCTTCT ACTCCTCCCC TAGTCAGGAA GTTCCCCCCC GCCCCGCAGC TCGCGTCGTG CAGGACGTGA CAAATGGAAG TAGCACGTCT CACTAGTCTC GTGCAGATGG ACAGCACCGC TGAGCAATGG AAGCGGGTAG GCCTTTGGGG CAGCGGCCAA TAGCAGCTTT GCTCCTTCGC TTTCTGGGCT CAGAGGCTGG GAAGGGGTGG GTCCGGGGGC GGGCTCAGGG GCGGGCTCAG GGGCGGGGCG GGCGCCCGAA GGTCCTCCGG AGGCCCGGCA TTCTGCACGC TTCAAAAGCG CACGTCTGCC GCGCTGTTCT CCTCTTCCTC ATCTCCGGGC CTTTCGACCT GCAGCCCAAG CTTACCATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGAATCG ATAGATCCTA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCCT GAGATCCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAG ACAAGATCTG CTTTTTGCTT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGTAG TAGTTCATGT CATCTTATTA TTCAGTATTT ATAACTTGCA AAGAAATGAA TATCAGAGAG TGAGAGGCCC GGGTTAATTA AGGAAAGGGC TAGATCATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCAAGCTCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCCGTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC ATGATTACGA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCAACTGGA TAACTCAAGC TAACCAAAAT CATCCCAAAC TTCCCACCCC ATACCCTATT ACCACTGCCA ATTACCTGTG GTTTCATTTA CTCTAAACCT GTGATTCCTC TGAATTATTT TCATTTTAAA GAAATTGTAT TTGTTAAATA TGTACTACAA ACTTAGTAGT
