pADV-mCMV-EGFP-CMV-Slc1a5(rat)-3×FLAG
$70.00
In stock
SKU
PL40077
NM_175758.3;Asct2; H4-ASCT2; Slc1a7
100μg liquid plasmid(Instantly):$91
| name | pADV-mCMV-EGFP-CMV-Slc1a5(rat)-3×FLAG | another name | apADV-mCMV-EGFP-CMV-Slc1a5(rat)-3×FLAG |
| Insert Size (bp) | 1617bp | Plasmid host | Mammalian cell,Adenovirus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Rat | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | mCMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTCATCAATA ATATACCTTA TTTTGGATTG AAGCCAATAT GATAATGAGG GGGTGGAGTT TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG ACTTTGACCG TTTACGTGGA GACTCGCCCA GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC GGGTCAAAGT TGGCGTTTTA TTATTATAGT CAGTCTAGAG ATATACTGAG TCATTAGGGA CTTTCCAATG GGTTTTGCCC AGTACATAAG GTCAATAGGG GTGAATCAAC AGGAAAGTCC CATTGGAGCC AAGTACACTG AGTCAATAGG GACTTTCCAT TGGGTTTTGC CCAGTACAAA AGGTCAATAG GGGGTGAGTC AATGGGTTTT TCCCATTATT GGCACGTACA TAAGGTCAAT AGGGGTGAGT CATTGGGTTT TTCCAGCCAA TTTAATTAAA ACGCCATGTA CTTTCCCACC ATTGACGTCA ATGGGCTATT GAAACTAATG CAACGTGACC TTTAAACGGT ACTTTCCCAT AGCTGATTAA TGGGAAAGTA CCGTTCTCGA GCCAATACAC GTCAATGGGA AGTGAAAGGG CAGCCAAAAC GTAACACCGC CCCGGTTTTC CCCTGGAAAT TCCATATTGG CACGCATTCT ATTGGCTGAG CTGCGTTCTA CGTGGGTATA AGAGGCGCGA CCAGCGTCGG TACCGTCGCA GTCTTCGGTC TGACCACCGT AGAACGCAGA TCGAATTCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCT CGAGATCCAC CGGATCTAGA TAACTGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAACGCGTT GACATTGATT ATTGACTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA ATACGACTCA CTATAGGGAG ACCCAAGCTG GCTAGCGCCA CCATGGCAGT GGATCCTCCT AAGGCTGACC CCAAAGGGGT GGTGGCGGTG GATCCCACCG CGAACTGTGG CTCGGGGCTG AAGTCCAGAG AGGACCAGGG AGCGAAAGCA GGTGGATGCT GCAGTTCCCG GGACCAAGTG TGCCGCTGCC TTCGCGCCAA CCTGCTGGTT CTGCTCACCG TGGCGGCGGC GGTGGCTGGC GTGGTGCTAG GTCTGGGGGT CTCGGCGGCG GGCGGTGCTG AGGCGCTGGG CCACGCGCGC TTTACCGCTT TCGCCTTCCC GGGAGAGCTG CTGTTGCGTC TGCTGGAGAT GATCATCCTG CCGCTGGTGG TGTGCAGCCT GATCGGAGGT GCAGCCAGTC TGGACCCTAG CGCGCTCGGC CGTTTGGGCG CCTGGGCCCT GCTCTTTTTC CTGGTCACCA CACTGCTCTC TTCGGCTCTC GGCGTGGCCT TGGCCCTGGC GCTGAAGCCG GGCGCCGCGT TTGCTGCCAT CAACTCCTCT GTTGTAGACT CCAGTGTCCA CAGAGCACCC ACCAAAGAGG TGCTGGATTC CTTTCTGGAA CTCCTGAGGA ATATGTTCCC CTCCAATCTG GTGTCTGCTT CTGCTGCCTT CCGCATTCCA TGTGGTGCCT GTCCACAGAG GAGCAATGCA ACCATGGACC AGCCTCACTG TGAGATGAAG ATGAACATTC TGGGCTTGGT CGTGTTCGCT ATAGTCTTTG GCGTGGCTCT GAGGAAGCTG GGGCCCGAGG GTGAGCTGCT CATCCGATTC TTCAACTCCT TCAATGATGC CACCATGGTC CTGGTCTCCT GGATTATGTG GTACGCCCCC ATTGGCATCT TGTTCCTGGT GGCCGGCAAG ATTGTGGAGA TGAAAGACAT CCGCCAGCTC TTCATCGGCC TCGGCAAATA CATCGTGTGC TGCCTGCTGG GCCACGCCAT CCACGGGCTC CTGGTTCTGC CTCTCATCTA CTTCCTCTTT ACCCGCAAAA ACCCTTATCG ATTCCTGTGG GGCATCGTGA CACCCCTGGC TACAGCTTTC GGGACCTCTT CTAGCTCTGC CACATTGCCC CTGATGATGA AGTGTGTAGA GGAGAAGAAT GGTGTGGCCA AACACATCAG CCGGTTCATC CTGCCCATCG GCGCCACCGT CAATATGGAC GGGGCAGCGT TGTTCCAGTG CGTGGCGGCA GTGTTCATTG CACAACTAAA TGGGATGTCC CTGGACTTCG TGAAGATCAT CACCATCCTG GTCACAGCTA CTGCATCCAG TGTCGGGGCC GCAGGTATCC CTGCAGGGGG CGTCCTCACT CTTGCCATCA TTCTAGAAGC AATCAGCCTC CCTGTCAAGG ACATCTCCTT GATCTTGGCC GTGGACTGGC TAGTGGACAG GTCCTGTACG GTCCTCAACG TGGAGGGTGA TGCTTTTGGG GCCGGATTGC TCCAGAGTTA CGTGGATCGC ACCAAGATGC CGAGCTCAGA GCCTGAACTG ATCCAGGTGA AGAACGACGT GTCTCTGAAA CCATTGCCCC TCGCCACAGA GGAGGGGAAC CCCCTTCTGA AACAGTGCCG GGAACCCTCC GGGGACTCCA GTGCCACATG CGAAAAGGAA TCTGTCATGA AGCTTGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA GAAGTTTAAA CCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGGTCGACTT CGAGCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC GTCTAGCATC GAAGATCCAA TAACTTCGTA TAGCATACAT TATACGAAGT TATAAGTAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AACACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACTCTAGGCA AAATAGCACC CTCCCGCTCC AGAACAACAT ACAGCGCTTC ACAGCGGCAG CCTAACAGTC AGCCTTACCA GTAAAAAAGA AAACCTATTA AAAAAACACC ACTCGACACG GCACCAGCTC AATCAGTCAC AGTGTAAAAA AGGGCCAAGT GCAGAGCGAG TATATATAGG ACTAAAAAAT GACGTAACGG TTAAAGTCCA CAAAAAACAC CCAGAAAACC GCACGCGAAC CTACGCCCAG AAACGAAAGC CAAAAAACCC ACAACTTCCT CAAATCGTCA CTTCCGTTTT CCCACGTTAC GTAACTTCCC ATTTTAAGAA AACTACAATT CCCAACACAT ACAAGTTACT CCGCCCTAAA ACCTACGTCA CCCGCCCCGT TCCCACGCCC CGCGCCACGT CACAAACTCC ACCCCCTCAT TATCATATTG GCTTCAATCC AAAATAAGGT ATA
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