pCAMBIA2300-MCS-GUS
$128.00
In stock
SKU
PL39984
100μg liquid plasmid(Instantly):$171
| name | pCAMBIA2300-MCS-GUS | another name | apCAMBIA2300-MCS-GUS |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Gus |
| Promoter | Replicon | pVS1 | |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CATGCCAACC ACAGGGTTCC CCTCGGGATC AAAGTACTTT GATCCAACCC CTCCGCTGCT ATAGTGCAGT CGGCTTCTGA CGTTCAGTGC AGCCGTCTTC TGAAAACGAC ATGTCGCACA AGTCCTAAGT TACGCGACAG GCTGCCGCCC TGCCCTTTTC CTGGCGTTTT CTTGTCGCGT GTTTTAGTCG CATAAAGTAG AATACTTGCG ACTAGAACCG GAGACATTAC GCCATGAACA AGAGCGCCGC CGCTGGCCTG CTGGGCTATG CCCGCGTCAG CACCGACGAC CAGGACTTGA CCAACCAACG GGCCGAACTG CACGCGGCCG GCTGCACCAA GCTGTTTTCC GAGAAGATCA CCGGCACCAG GCGCGACCGC CCGGAGCTGG CCAGGATGCT TGACCACCTA CGCCCTGGCG ACGTTGTGAC AGTGACCAGG CTAGACCGCC TGGCCCGCAG CACCCGCGAC CTACTGGACA TTGCCGAGCG CATCCAGGAG GCCGGCGCGG GCCTGCGTAG CCTGGCAGAG CCGTGGGCCG ACACCACCAC GCCGGCCGGC CGCATGGTGT TGACCGTGTT CGCCGGCATT GCCGAGTTCG AGCGTTCCCT AATCATCGAC CGCACCCGGA GCGGGCGCGA GGCCGCCAAG GCCCGAGGCG TGAAGTTTGG CCCCCGCCCT ACCCTCACCC CGGCACAGAT CGCGCACGCC CGCGAGCTGA TCGACCAGGA AGGCCGCACC GTGAAAGAGG CGGCTGCACT GCTTGGCGTG CATCGCTCGA CCCTGTACCG CGCACTTGAG CGCAGCGAGG AAGTGACGCC CACCGAGGCC AGGCGGCGCG GTGCCTTCCG TGAGGACGCA TTGACCGAGG CCGACGCCCT GGCGGCCGCC GAGAATGAAC GCCAAGAGGA ACAAGCATGA AACCGCACCA GGACGGCCAG GACGAACCGT TTTTCATTAC CGAAGAGATC GAGGCGGAGA TGATCGCGGC CGGGTACGTG TTCGAGCCGC CCGCGCACGT CTCAACCGTG CGGCTGCATG AAATCCTGGC CGGTTTGTCT GATGCCAAGC TGGCGGCCTG GCCGGCCAGC TTGGCCGCTG AAGAAACCGA GCGCCGCCGT CTAAAAAGGT GATGTGTATT TGAGTAAAAC AGCTTGCGTC ATGCGGTCGC TGCGTATATG ATGCGATGAG TAAATAAACA AATACGCAAG GGGAACGCAT GAAGGTTATC GCTGTACTTA ACCAGAAAGG CGGGTCAGGC AAGACGACCA TCGCAACCCA TCTAGCCCGC GCCCTGCAAC TCGCCGGGGC CGATGTTCTG TTAGTCGATT CCGATCCCCA GGGCAGTGCC CGCGATTGGG CGGCCGTGCG GGAAGATCAA CCGCTAACCG TTGTCGGCAT CGACCGCCCG ACGATTGACC GCGACGTGAA GGCCATCGGC CGGCGCGACT TCGTAGTGAT CGACGGAGCG CCCCAGGCGG CGGACTTGGC TGTGTCCGCG ATCAAGGCAG CCGACTTCGT GCTGATTCCG GTGCAGCCAA GCCCTTACGA CATATGGGCC ACCGCCGACC TGGTGGAGCT GGTTAAGCAG CGCATTGAGG TCACGGATGG AAGGCTACAA GCGGCCTTTG TCGTGTCGCG GGCGATCAAA GGCACGCGCA TCGGCGGTGA GGTTGCCGAG GCGCTGGCCG GGTACGAGCT GCCCATTCTT GAGTCCCGTA TCACGCAGCG CGTGAGCTAC CCAGGCACTG CCGCCGCCGG CACAACCGTT CTTGAATCAG AACCCGAGGG CGACGCTGCC CGCGAGGTCC AGGCGCTGGC CGCTGAAATT AAATCAAAAC TCATTTGAGT TAATGAGGTA AAGAGAAAAT GAGCAAAAGC ACAAACACGC TAAGTGCCGG CCGTCCGAGC GCACGCAGCA GCAAGGCTGC AACGTTGGCC AGCCTGGCAG ACACGCCAGC CATGAAGCGG GTCAACTTTC AGTTGCCGGC GGAGGATCAC ACCAAGCTGA AGATGTACGC GGTACGCCAA GGCAAGACCA TTACCGAGCT GCTATCTGAA TACATCGCGC AGCTACCAGA GTAAATGAGC AAATGAATAA ATGAGTAGAT GAATTTTAGC GGCTAAAGGA GGCGGCATGG AAAATCAAGA ACAACCAGGC ACCGACGCCG TGGAATGCCC CATGTGTGGA GGAACGGGCG GTTGGCCAGG CGTAAGCGGC TGGGTTGTCT GCCGGCCCTG CAATGGCACT GGAACCCCCA AGCCCGAGGA ATCGGCGTGA GCGGTCGCAA ACCATCCGGC CCGGTACAAA TCGGCGCGGC GCTGGGTGAT GACCTGGTGG AGAAGTTGAA GGCCGCGCAG GCCGCCCAGC GGCAACGCAT CGAGGCAGAA GCACGCCCCG GTGAATCGTG GCAAGCGGCC GCTGATCGAA TCCGCAAAGA ATCCCGGCAA CCGCCGGCAG CCGGTGCGCC GTCGATTAGG AAGCCGCCCA AGGGCGACGA GCAACCAGAT TTTTTCGTTC CGATGCTCTA TGACGTGGGC ACCCGCGATA GTCGCAGCAT CATGGACGTG GCCGTTTTCC GTCTGTCGAA GCGTGACCGA CGAGCTGGCG AGGTGATCCG CTACGAGCTT CCAGACGGGC ACGTAGAGGT TTCCGCAGGG CCGGCCGGCA TGGCCAGTGT GTGGGATTAC GACCTGGTAC TGATGGCGGT TTCCCATCTA ACCGAATCCA TGAACCGATA CCGGGAAGGG AAGGGAGACA AGCCCGGCCG CGTGTTCCGT CCACACGTTG CGGACGTACT CAAGTTCTGC CGGCGAGCCG ATGGCGGAAA GCAGAAAGAC GACCTGGTAG AAACCTGCAT TCGGTTAAAC ACCACGCACG TTGCCATGCA GCGTACGAAG AAGGCCAAGA ACGGCCGCCT GGTGACGGTA TCCGAGGGTG AAGCCTTGAT TAGCCGCTAC AAGATCGTAA AGAGCGAAAC CGGGCGGCCG GAGTACATCG AGATCGAGCT AGCTGATTGG ATGTACCGCG AGATCACAGA AGGCAAGAAC CCGGACGTGC TGACGGTTCA CCCCGATTAC TTTTTGATCG ATCCCGGCAT CGGCCGTTTT CTCTACCGCC TGGCACGCCG CGCCGCAGGC AAGGCAGAAG CCAGATGGTT GTTCAAGACG ATCTACGAAC GCAGTGGCAG CGCCGGAGAG TTCAAGAAGT TCTGTTTCAC CGTGCGCAAG CTGATCGGGT CAAATGACCT GCCGGAGTAC GATTTGAAGG AGGAGGCGGG GCAGGCTGGC CCGATCCTAG TCATGCGCTA CCGCAACCTG ATCGAGGGCG AAGCATCCGC CGGTTCCTAA TGTACGGAGC AGATGCTAGG GCAAATTGCC CTAGCAGGGG AAAAAGGTCG AAAAGGTCTC TTTCCTGTGG ATAGCACGTA CATTGGGAAC CCAAAGCCGT ACATTGGGAA CCGGAACCCG TACATTGGGA ACCCAAAGCC GTACATTGGG AACCGGTCAC ACATGTAAGT GACTGATATA AAAGAGAAAA AAGGCGATTT TTCCGCCTAA AACTCTTTAA AACTTATTAA AACTCTTAAA ACCCGCCTGG CCTGTGCATA ACTGTCTGGC CAGCGCACAG CCGAAGAGCT GCAAAAAGCG CCTACCCTTC GGTCGCTGCG CTCCCTACGC CCCGCCGCTT CGCGTCGGCC TATCGCGGCC GCTGGCCGCT CAAAAATGGC TGGCCTACGG CCAGGCAATC TACCAGGGCG CGGACAAGCC GCGCCGTCGC CACTCGACCG CCGGCGCCCA CATCAAGGCA CCCTGCCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA TAGCGGAGTG TATACTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGCATTCTA GGTACTAAAA CAATTCATCC AGTAAAATAT AATATTTTAT TTTCTCCCAA TCAGGCTTGA TCCCCAGTAA GTCAAAAAAT AGCTCGACAT ACTGTTCTTC CCCGATATCC TCCCTGATCG ACCGGACGCA GAAGGCAATG TCATACCACT TGTCCGCCCT GCCGCTTCTC CCAAGATCAA TAAAGCCACT TACTTTGCCA TCTTTCACAA AGATGTTGCT GTCTCCCAGG TCGCCGTGGG AAAAGACAAG TTCCTCTTCG GGCTTTTCCG TCTTTAAAAA ATCATACAGC TCGCGCGGAT CTTTAAATGG AGTGTCTTCT TCCCAGTTTT CGCAATCCAC ATCGGCCAGA TCGTTATTCA GTAAGTAATC CAATTCGGCT AAGCGGCTGT CTAAGCTATT CGTATAGGGA CAATCCGATA TGTCGATGGA GTGAAAGAGC CTGATGCACT CCGCATACAG CTCGATAATC TTTTCAGGGC TTTGTTCATC TTCATACTCT TCCGAGCAAA GGACGCCATC GGCCTCACTC ATGAGCAGAT TGCTCCAGCC ATCATGCCGT TCAAAGTGCA GGACCTTTGG AACAGGCAGC TTTCCTTCCA GCCATAGCAT CATGTCCTTT TCCCGTTCCA CATCATAGGT GGTCCCTTTA TACCGGCTGT CCGTCATTTT TAAATATAGG TTTTCATTTT CTCCCACCAG CTTATATACC TTAGCAGGAG ACATTCCTTC CGTATCTTTT ACGCAGCGGT ATTTTTCGAT CAGTTTTTTC AATTCCGGTG ATATTCTCAT TTTAGCCATT TATTATTTCC TTCCTCTTTT CTACAGTATT TAAAGATACC CCAAGAAGCT AATTATAACA AGACGAACTC CAATTCACTG TTCCTTGCAT TCTAAAACCT TAAATACCAG AAAACAGCTT TTTCAAAGTT GTTTTCAAAG TTGGCGTATA ACATAGTATC GACGGAGCCG ATTTTGAAAC CGCGGTGATC ACAGGCAGCA ACGCTCTGTC ATCGTTACAA TCAACATGCT ACCCTCCGCG AGATCATCCG TGTTTCAAAC CCGGCAGCTT AGTTGCCGTT CTTCCGAATA GCATCGGTAA CATGAGCAAA GTCTGCCGCC TTACAACGGC TCTCCCGCTG ACGCCGTCCC GGACTGATGG GCTGCCTGTA TCGAGTGGTG ATTTTGTGCC GAGCTGCCGG TCGGGGAGCT GTTGGCTGGC TGGTGGCAGG ATATATTGTG GTGTAAACAA ATTGACGCTT AGACAACTTA ATAACACATT GCGGACGTTT TTAATGTACT GAATTAACGC CGAATTAATT CGGGGGATCT GGATTTTAGT ACTGGATTTT GGTTTTAGGA ATTAGAAATT TTATTGATAG AAGTATTTTA CAAATACAAA TACATACTAA GGGTTTCTTA TATGCTCAAC ACATGAGCGA AACCCTATAG GAACCCTAAT TCCCTTATCT GGGAACTACT CACACATTAT TATGGAGAAA CTCGAGCTTG TCGATCGACT CTAGCTAGAG GATCGATCCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGGTCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG TGTCACGACG AGATCCTCGC CGTCGGGCAT GCGCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCCT GGAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCC CCATGGTCGA TCGACAGATC TGCGAAAGCT CGAGAGAGAT AGATTTGTAG AGAGAGACTG GTGATTTCAG CGTGTCCTCT CCAAATGAAA TGAACTTCCT TATATAGAGG AAGGTCTTGC GAAGGATAGT GGGATTGTGC GTCATCCCTT ACGTCAGTGG AGATATCACA TCAATCCACT TGCTTTGAAG ACGTGGTTGG AACGTCTTCT TTTTCCACGA TGCTCCTCGT GGGTGGGGGT CCATCTTTGG GACCACTGTC GGCAGAGGCA TCTTGAACGA TAGCCTTTCC TTTATCGCAA TGATGGCATT TGTAGGTGCC ACCTTCCTTT TCTACTGTCC TTTTGATGAA GTGACAGATA GCTGGGCAAT GGAATCCGAG GAGGTTTCCC GATATTACCC TTTGTTGAAA AGTCTCAATA GCCCTTTGGT CTTCTGAGAC TGTATCTTTG ATATTCTTGG AGTAGACGAG AGTGTCGTGC TCCACCATGT TATCACATCA ATCCACTTGC TTTGAAGACG TGGTTGGAAC GTCTTCTTTT TCCACGATGC TCCTCGTGGG TGGGGGTCCA TCTTTGGGAC CACTGTCGGC AGAGGCATCT TGAACGATAG CCTTTCCTTT ATCGCAATGA TGGCATTTGT AGGTGCCACC TTCCTTTTCT ACTGTCCTTT TGATGAAGTG ACAGATAGCT GGGCAATGGA ATCCGAGGAG GTTTCCCGAT ATTACCCTTT GTTGAAAAGT CTCAATAGCC CTTTGGTCTT CTGAGACTGT ATCTTTGATA TTCTTGGAGT AGACGAGAGT GTCGTGCTCC ACCATGTTGG CAAGCTGCTC TAGCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGA ATTCGAGCTC GGTACCCGGG GATCCATGGT AGATCTGAGG GTAAATTTCT AGTTTTTCTC CTTCATTTTC TTGGTTAGGA CCCTTTTCTC TTTTTATTTT TTTGAGCTTT GATCTTTCTT TAAACTGATC TATTTTTTAA TTGATTGGTT ATGGTGTAAA TATTACATAG CTTTAACTGA TAATCTGATT ACTTTATTTC GTGTGTCTAT GATGATGATG ATAGTTACAG AACCGACGAC TCGTCCGTCC TGTAGAAACC CCAACCCGTG AAATCAAAAA ACTCGACGGC CTGTGGGCAT TCAGTCTGGA TCGCGAAAAC TGTGGAATTG ATCAGCGTTG GTGGGAAAGC GCGTTACAAG AAAGCCGGGC AATTGCTGTG CCAGGCAGTT TTAACGATCA GTTCGCCGAT GCAGATATTC GTAATTATGC GGGCAACGTC TGGTATCAGC GCGAAGTCTT TATACCGAAA GGTTGGGCAG GCCAGCGTAT CGTGCTGCGT TTCGATGCGG TCACTCATTA CGGCAAAGTG TGGGTCAATA ATCAGGAAGT GATGGAGCAT CAGGGCGGCT ATACGCCATT TGAAGCCGAT GTCACGCCGT ATGTTATTGC CGGGAAAAGT GTACGTATCA CCGTTTGTGT GAACAACGAA CTGAACTGGC AGACTATCCC GCCGGGAATG GTGATTACCG ACGAAAACGG CAAGAAAAAG CAGTCTTACT TCCATGATTT CTTTAACTAT GCCGGAATCC ATCGCAGCGT AATGCTCTAC ACCACGCCGA ACACCTGGGT GGACGATATC ACCGTGGTGA CGCATGTCGC GCAAGACTGT AACCACGCGT CTGTTGACTG GCAGGTGGTG GCCAATGGTG ATGTCAGCGT TGAACTGCGT GATGCGGATC AACAGGTGGT TGCAACTGGA CAAGGCACTA GCGGGACTTT GCAAGTGGTG AATCCGCACC TCTGGCAACC GGGTGAAGGT TATCTCTATG AACTGTGCGT CACAGCCAAA AGCCAGACAG AGTGTGATAT CTACCCGCTT CGCGTCGGCA TCCGGTCAGT GGCAGTGAAG GGCGAACAGT TCCTGATTAA CCACAAACCG TTCTACTTTA CTGGCTTTGG TCGTCATGAA GATGCGGACT TGCGTGGCAA AGGATTCGAT AACGTGCTGA TGGTGCACGA CCACGCATTA ATGGACTGGA TTGGGGCCAA CTCCTACCGT ACCTCGCATT ACCCTTACGC TGAAGAGATG CTCGACTGGG CAGATGAACA TGGCATCGTG GTGATTGATG AAACTGCTGC TGTCGGCTTT AACCTCTCTT TAGGCATTGG TTTCGAAGCG GGCAACAAGC CGAAAGAACT GTACAGCGAA GAGGCAGTCA ACGGGGAAAC TCAGCAAGCG CACTTACAGG CGATTAAAGA GCTGATAGCG CGTGACAAAA ACCACCCAAG CGTGGTGATG TGGAGTATTG CCAACGAACC GGATACCCGT CCGCAAGGTG CACGGGAATA TTTCGCGCCA CTGGCGGAAG CAACGCGTAA ACTCGACCCG ACGCGTCCGA TCACCTGCGT CAATGTAATG TTCTGCGACG CTCACACCGA TACCATCAGC GATCTCTTTG ATGTGCTGTG CCTGAACCGT TATTACGGAT GGTATGTCCA AAGCGGCGAT TTGGAAACGG CAGAGAAGGT ACTGGAAAAA GAACTTCTGG CCTGGCAGGA GAAACTGCAT CAGCCGATTA TCATCACCGA ATACGGCGTG GATACGTTAG CCGGGCTGCA CTCAATGTAC ACCGACATGT GGAGTGAAGA GTATCAGTGT GCATGGCTGG ATATGTATCA CCGCGTCTTT GATCGCGTCA GCGCCGTCGT CGGTGAACAG GTATGGAATT TCGCCGATTT TGCGACCTCG CAAGGCATAT TGCGCGTTGG CGGTAACAAG AAAGGGATCT TCACTCGCGA CCGCAAACCG AAGTCGGCGG CTTTTCTGCT GCAAAAACGC TGGACTGGCA TGAACTTCGG TGAAAAACCG CAGCAGGGAG GCAAACAAGC TAGCCACCAC CACCACCACC ACGTGTGAAT TACAGGTGAC CAGCTCGAAT TTCCCCGATC GTTCAAACAT TTGGCAATAA AGTTTCTTAA GATTGAATCC TGTTGCCGGT CTTGCGATGA TTATCATATA ATTTCTGTTG AATTACGTTA AGCATGTAAT AATTAACATG TAATGCATGA CGTTATTTAT GAGATGGGTT TTTATGATTA GAGTCCCGCA ATTATACATT TAATACGCGA TAGAAAACAA AATATAGCGC GCAAACTAGG ATAAATTATC GCGCGCGGTG TCATCTATGT TACTAGATCG TCGACCTGCA GGCATGCAAG CTTGGCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGCTAGAGCA GCTTGAGCTT GGATCAGATT GTCGTTTCCC GCCTTCAGTT TAAACTATCA GTGTTTGACA GGATATATTG GCGGGTAAAC CTAAGAGAAA AGAGCGTTTA TTAGAATAAC GGATATTTAA AAGGGCGTGA AAAGGTTTAT CCGTTCGTCC ATTTGTATGT G
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