NM_001111.5;ADAR1; AGS6; DRADA; DSH; DSRAD; G1P1
100μg liquid plasmid(Instantly):$134
| name | pBiFC-CMV-FLAG-ADAR(human)-CrN173 | another name | apBiFC-CMV-FLAG-ADAR(human)-CrN173 |
| Insert Size (bp) | 3678bp | Plasmid host | Mammary cell |
| Plasmid uses | Bimolecular fluorescence | Fragment type | BIFC |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TACCATGGAC TACAAAGACG ATGACGACAA GCTTATGAAT CCGCGGCAGG GGTATTCCCT CAGCGGATAC TACACCCATC CATTTCAAGG CTATGAGCAC AGACAGCTCA GGTACCAGCA GCCTGGGCCA GGATCTTCCC CCAGTAGTTT CCTGCTTAAG CAAATAGAAT TTCTCAAGGG GCAGCTCCCA GAAGCACCGG TGATTGGAAA GCAGACACCG TCACTGCCAC CTTCCCTCCC AGGACTCCGG CCAAGGTTTC CAGTACTACT TGCCTCCAGT ACCAGAGGCA GGCAAGTGGA CATCAGGGGT GTCCCCAGGG GCGTGCATCT CAGAAGTCAG GGGCTCCAGA GAGGGTTCCA GCATCCTTCA CCACGTGGCA GGAGTCTGCC ACAGAGAGGT GTTGATTGCC TTTCCTCACA TTTCCAGGAA CTGAGTATCT ACCAAGATCA GGAACAAAGG ATCTTAAAGT TCCTGGAAGA GCTTGGGGAA GGGAAGGCCA CCACAGCACA TGATCTGTCT GGGAAACTTG GGACTCCGAA GAAAGAAATC AATCGAGTTT TATACTCCCT GGCAAAGAAG GGCAAGCTAC AGAAAGAGGC AGGAACACCC CCTTTGTGGA AAATCGCGGT CTCCACTCAG GCTTGGAACC AGCACAGCGG AGTGGTAAGA CCAGACGGTC ATAGCCAAGG AGCCCCAAAC TCAGACCCGA GTTTGGAACC GGAAGACAGA AACTCCACAT CTGTCTCAGA AGATCTTCTT GAGCCTTTTA TTGCAGTCTC AGCTCAGGCT TGGAACCAGC ACAGCGGAGT GGTAAGACCA GACAGTCATA GCCAAGGATC CCCAAACTCA GACCCAGGTT TGGAACCTGA AGACAGCAAC TCCACATCTG CCTTGGAAGA TCCTCTTGAG TTTTTAGACA TGGCCGAGAT CAAGGAGAAA ATCTGCGACT ATCTCTTCAA TGTGTCTGAC TCCTCTGCCC TGAATTTGGC TAAAAATATT GGCCTTACCA AGGCCCGAGA TATAAATGCT GTGCTAATTG ACATGGAAAG GCAGGGGGAT GTCTATAGAC AAGGGACAAC CCCTCCCATA TGGCATTTGA CAGACAAGAA GCGAGAGAGG ATGCAAATCA AGAGAAATAC GAACAGTGTT CCTGAAACCG CTCCAGCTGC AATCCCTGAG ACCAAAAGAA ACGCAGAGTT CCTCACCTGT AATATACCCA CATCAAATGC CTCAAATAAC ATGGTAACCA CAGAAAAAGT GGAGAATGGG CAGGAACCTG TCATAAAGTT AGAAAACAGG CAAGAGGCCA GACCAGAACC AGCAAGACTG AAACCACCTG TTCATTACAA TGGCCCCTCA AAAGCAGGGT ATGTTGACTT TGAAAATGGC CAGTGGGCCA CAGATGACAT CCCAGATGAC TTGAATAGTA TCCGCGCAGC ACCAGGTGAG TTTCGAGCCA TCATGGAGAT GCCCTCCTTC TACAGTCATG GCTTGCCACG GTGTTCACCC TACAAGAAAC TGACAGAGTG CCAGCTGAAG AACCCCATCA GCGGGCTGTT AGAATATGCC CAGTTCGCTA GTCAAACCTG TGAGTTCAAC ATGATAGAGC AGAGTGGACC ACCCCATGAA CCTCGATTTA AATTCCAGGT TGTCATCAAT GGCCGAGAGT TTCCCCCAGC TGAAGCTGGA AGCAAGAAAG TGGCCAAGCA GGATGCAGCT ATGAAAGCCA TGACAATTCT GCTAGAGGAA GCCAAAGCCA AGGACAGTGG AAAATCAGAA GAATCATCCC ACTATTCCAC AGAGAAAGAA TCAGAGAAGA CTGCAGAGTC CCAGACCCCC ACCCCTTCAG CCACATCCTT CTTTTCTGGG AAGAGCCCCG TCACCACACT GCTTGAGTGT ATGCACAAAT TGGGGAACTC CTGCGAATTC CGTCTCCTGT CCAAAGAAGG CCCTGCCCAT GAACCCAAGT TCCAATACTG TGTTGCAGTG GGAGCCCAAA CTTTCCCCAG TGTGAGTGCT CCCAGCAAGA AAGTGGCAAA GCAGATGGCC GCAGAGGAAG CCATGAAGGC CCTGCATGGG GAGGCGACCA ACTCCATGGC TTCTGATAAC CAGCCTGAAG GTATGATCTC AGAGTCACTT GATAACTTGG AATCCATGAT GCCCAACAAG GTCAGGAAGA TTGGCGAGCT CGTGAGATAC CTGAACACCA ACCCTGTGGG TGGCCTTTTG GAGTACGCCC GCTCCCATGG CTTTGCTGCT GAATTCAAGT TGGTCGACCA GTCCGGACCT CCTCACGAGC CCAAGTTCGT TTACCAAGCA AAAGTTGGGG GTCGCTGGTT CCCAGCCGTC TGCGCACACA GCAAGAAGCA AGGCAAGCAG GAAGCAGCAG ATGCGGCTCT CCGTGTCTTG ATTGGGGAGA ACGAGAAGGC AGAACGCATG GGTTTCACAG AGGTAACCCC AGTGACAGGG GCCAGTCTCA GAAGAACTAT GCTCCTCCTC TCAAGGTCCC CAGAAGCACA GCCAAAGACA CTCCCTCTCA CTGGCAGCAC CTTCCATGAC CAGATAGCCA TGCTGAGCCA CCGGTGCTTC AACACTCTGA CTAACAGCTT CCAGCCCTCC TTGCTCGGCC GCAAGATTCT GGCCGCCATC ATTATGAAAA AAGACTCTGA GGACATGGGT GTCGTCGTCA GCTTGGGAAC AGGGAATCGC TGTGTGAAAG GAGATTCTCT CAGCCTAAAA GGAGAAACTG TCAATGACTG CCATGCAGAA ATAATCTCCC GGAGAGGCTT CATCAGGTTT CTCTACAGTG AGTTAATGAA ATACAACTCC CAGACTGCGA AGGATAGTAT ATTTGAACCT GCTAAGGGAG GAGAAAAGCT CCAAATAAAA AAGACTGTGT CATTCCATCT GTATATCAGC ACTGCTCCGT GTGGAGATGG CGCCCTCTTT GACAAGTCCT GCAGCGACCG TGCTATGGAA AGCACAGAAT CCCGCCACTA CCCTGTCTTC GAGAATCCCA AACAAGGAAA GCTCCGCACC AAGGTGGAGA ACGGAGAAGG CACAATCCCT GTGGAATCCA GTGACATTGT GCCTACGTGG GATGGCATTC GGCTCGGGGA GAGACTCCGT ACCATGTCCT GTAGTGACAA AATCCTACGC TGGAACGTGC TGGGCCTGCA AGGGGCACTG TTGACCCACT TCCTGCAGCC CATTTATCTC AAATCTGTCA CATTGGGTTA CCTTTTCAGC CAAGGGCATC TGACCCGTGC TATTTGCTGT CGTGTGACAA GAGATGGGAG TGCATTTGAG GATGGACTAC GACATCCCTT TATTGTCAAC CACCCCAAGG TTGGCAGAGT CAGCATATAT GATTCCAAAA GGCAATCCGG GAAGACTAAG GAGACAAGCG TCAACTGGTG TCTGGCTGAT GGCTATGACC TGGAGATCCT GGACGGTACC AGAGGCACTG TGGATGGGCC ACGGAATGAA TTGTCCCGGG TCTCCAAAAA GAACATTTTT CTTCTATTTA AGAAGCTCTG CTCCTTCCGT TACCGCAGGG ATCTACTGAG ACTCTCCTAT GGTGAGGCCA AGAAAGCTGC CCGTGACTAC GAGACGGCCA AGAACTACTT CAAAAAAGGC CTGAAGGATA TGGGCTATGG GAACTGGATT AGCAAACCCC AGGAGGAAAA GAACTTTTAT CTCTGCCCAG TATCTAGAAG ATCCAGCGCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTGGGGCGTG CAGTGCTTCG CCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACGC CATCAGCGAC AACGTCTATA TCACCGCCGA CAAGCAGAAG AACGGCATCA AGGCCAACTT CAAGATCCGC CACAACATCG AGTAGGGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC TCGAGGAACT GAAAAACCAG AAAGTTAATT CCCTATAGTG AGTCGTATTA AATTCGTAAT CATGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG CCAAGCTGAT CTATACATTG AATCAATATT GGCAATTAGC CATATTAGTC ATTGGTTATA TAGCATAAAT CAATATTGGC TATTGGCCAT TGCATACGTT GTATCTATAT CATAATATGT ACATTTATAT TGGCTCATGT CCAATATGAC CGCCATGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTC CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTACGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ACCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAATAAC CCCGCCCCGT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GAATTGATC
