NM_004446.3;EARS; EPRS; GLUPRORS; HLD15; PARS; PIG32;QARS; QPRS
100μg liquid plasmid(Instantly):$134
| name | pCMV-EPRS1(human)-3×FLAG-Neo | another name | apCMV-EPRS1(human)-3×FLAG-Neo |
| Insert Size (bp) | 4536bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCATGGC GACGCTCTCT CTGACCGTGA ATTCAGGAGA CCCTCCGCTA GGAGCTTTGC TGGCAGTAGA ACACGTGAAA GACGATGTCA GCATTTCCGT TGAAGAAGGG AAAGAGAATA TTCTTCATGT TTCTGAAAAT GTGATATTCA CAGATGTGAA TTCTATACTT CGCTACTTGG CTAGAGTTGC AACTACAGCT GGGTTATATG GCTCTAATCT GATGGAACAT ACTGAGATTG ATCACTGGTT GGAGTTCAGT GCTACAAAAT TATCTTCATG TGATTCCTTT ACTTCTACAA TTAATGAACT CAATCATTGC CTGTCTCTGA GAACATACTT AGTTGGAAAC TCCTTGAGTT TAGCAGATTT ATGTGTTTGG GCCACCCTAA AAGGAAATGC TGCCTGGCAA GAACAGTTGA AACAGAAGAA AGCTCCAGTT CATGTAAAAC GTTGGTTTGG CTTTCTTGAA GCCCAGCAGG CCTTCCAGTC AGTAGGTACC AAGTGGGATG TTTCAACAAC CAAAGCTCGA GTGGCACCTG AGAAAAAGCA AGATGTTGGG AAATTTGTTG AGCTTCCAGG TGCGGAGATG GGAAAGGTTA CCGTCAGATT TCCTCCAGAG GCCAGTGGTT ACTTACACAT TGGGCATGCA AAAGCTGCTC TTCTGAACCA GCACTACCAG GTTAACTTTA AAGGGAAACT GATCATGAGA TTTGATGACA CAAATCCTGA AAAAGAAAAG GAAGATTTTG AGAAGGTTAT CTTGGAAGAT GTTGCAATGT TGCATATCAA ACCAGATCAA TTTACTTATA CTTCGGATCA TTTTGAAACT ATAATGAAGT ATGCAGAGAA GCTAATTCAA GAAGGGAAGG CTTATGTGGA TGATACTCCT GCTGAACAGA TGAAAGCAGA ACGTGAGCAG AGGATAGACT CTAAACATAG AAAAAACCCT ATTGAGAAGA ATCTACAAAT GTGGGAAGAA ATGAAAAAAG GGAGCCAGTT TGGTCAGTCC TGTTGTTTGC GAGCAAAAAT TGACATGAGT AGTAACAATG GATGCATGAG AGATCCAACC CTTTATCGCT GCAAAATTCA ACCACATCCA AGAACTGGAA ATAAATACAA TGTTTATCCA ACATATGATT TTGCCTGCCC CATAGTTGAC AGCATCGAAG GTGTTACACA TGCCCTGAGA ACAACAGAAT ACCATGACAG AGATGAGCAG TTTTACTGGA TTATTGAAGC TTTAGGCATA AGAAAACCAT ATATTTGGGA ATATAGTCGG CTAAATCTCA ACAACACAGT GCTATCCAAA AGAAAACTCA CATGGTTTGT CAATGAAGGA CTAGTAGATG GATGGGATGA CCCAAGATTT CCTACGGTTC GTGGTGTACT GAGAAGAGGG ATGACAGTTG AAGGACTGAA ACAGTTTATT GCTGCTCAGG GCTCCTCACG TTCAGTCGTG AACATGGAGT GGGACAAAAT CTGGGCGTTT AACAAAAAGG TTATTGACCC AGTGGCTCCA CGATATGTTG CATTACTGAA GAAAGAAGTG ATCCCAGTGA ATGTACCTGA AGCTCAGGAG GAGATGAAAG AAGTAGCCAA ACACCCAAAG AATCCTGAGG TTGGCTTGAA GCCTGTGTGG TATAGTCCCA AAGTTTTCAT TGAAGGTGCT GATGCAGAGA CTTTTTCGGA GGGTGAGATG GTTACATTTA TAAATTGGGG CAACCTCAAC ATTACAAAAA TACACAAAAA TGCAGATGGA AAAATCATAT CTCTTGATGC AAAGTTGAAT TTGGAAAACA AAGACTACAA GAAAACCACT AAGGTCACTT GGCTTGCAGA GACTACACAT GCTCTTCCTA TTCCAGTAAT CTGTGTCACT TATGAGCACT TGATCACAAA GCCAGTGCTA GGAAAAGACG AGGACTTTAA GCAGTATGTC AACAAGAACA GTAAGCATGA AGAGCTAATG CTAGGGGATC CCTGCCTTAA GGATTTGAAA AAAGGAGATA TTATACAACT CCAGAGAAGA GGATTCTTCA TATGTGATCA ACCTTATGAA CCTGTTAGCC CATATAGTTG CAAGGAAGCC CCGTGTGTTT TGATATACAT TCCTGATGGG CACACAAAGG AAATGCCAAC ATCAGGGTCA AAGGAAAAGA CCAAAGTAGA AGCCACAAAA AATGAGACCT CTGCTCCTTT TAAGGAAAGA CCAACACCTT CTCTGAATAA TAATTGTACT ACATCTGAGG ATTCCTTGGT CCTTTACAAT AGAGTGGCTG TTCAAGGAGA TGTGGTTCGT GAATTAAAAG CCAAGAAAGC ACCAAAGGAA GATGTAGATG CAGCTGTAAA ACAGCTTTTG TCTTTGAAAG CTGAATATAA GGAGAAAACT GGCCAGGAAT ATAAACCTGG AAACCCTCCT GCTGAAATAG GACAGAATAT TTCTTCTAAT TCCTCAGCAA GTATTCTGGA AAGTAAATCT CTGTATGATG AAGTTGCTGC ACAAGGGGAG GTGGTTCGTA AGCTAAAAGC TGAAAAATCC CCTAAGGCTA AAATAAATGA AGCTGTAGAA TGCTTACTGT CCCTGAAGGC TCAGTATAAA GAAAAAACTG GGAAGGAGTA CATACCTGGT CAGCCCCCAT TATCTCAAAG TTCGGATTCA AGCCCAACCA GAAATTCTGA ACCTGCTGGT TTAGAAACAC CAGAAGCGAA AGTACTTTTT GACAAAGTAG CTTCTCAAGG GGAAGTAGTT CGGAAACTTA AAACTGAAAA AGCCCCTAAG GATCAAGTAG ATATAGCTGT TCAAGAACTC CTTCAGCTAA AGGCACAGTA CAAGTCTTTG ATAGGAGTAG AGTATAAGCC TGTGTCGGCC ACTGGAGCTG AGGACAAAGA TAAGAAGAAG AAAGAAAAAG AAAATAAATC TGAAAAGCAG AATAAGCCTC AGAAACAAAA TGATGGCCAA AGGAAAGACC CTTCTAAAAA CCAAGGAGGT GGGCTCTCAT CAAGTGGAGC AGGAGAAGGG CAGGGGCCTA AGAAACAGAC CAGGTTGGGT CTTGAGGCAA AAAAAGAAGA AAATCTTGCT GATTGGTATT CTCAGGTCAT CACAAAGTCA GAAATGATTG AATACCATGA CATAAGTGGC TGTTATATTC TTCGTCCCTG GGCCTATGCC ATTTGGGAAG CCATCAAGGA CTTTTTTGAT GCTGAGATCA AGAAACTTGG TGTTGAAAAC TGCTACTTCC CCATGTTTGT GTCTCAAAGT GCATTAGAGA AAGAGAAGAC TCATGTTGCT GACTTTGCCC CAGAGGTTGC TTGGGTTACA AGATCTGGCA AAACCGAGCT GGCAGAACCA ATTGCCATTC GTCCTACTAG TGAAACAGTA ATGTATCCTG CATATGCAAA ATGGGTACAG TCACACAGAG ACCTGCCCAT CAAGCTCAAT CAGTGGTGCA ATGTGGTGCG TTGGGAATTC AAGCATCCTC AGCCTTTCCT ACGTACTCGT GAATTTCTTT GGCAGGAAGG GCACAGTGCT TTTGCTACCA TGGAAGAGGC AGCGGAAGAG GTCTTGCAGA TACTTGACTT ATATGCTCAG GTATATGAAG AACTCCTGGC AATTCCTGTT GTTAAAGGAA GAAAGACGGA AAAGGAAAAA TTTGCAGGAG GAGACTATAC AACTACAATA GAAGCATTTA TATCTGCTAG TGGAAGAGCT ATCCAGGGAG GAACATCACA TCATTTAGGG CAGAATTTTT CCAAAATGTT TGAAATCGTT TTTGAAGATC CAAAGATACC AGGAGAGAAG CAATTTGCCT ATCAAAACTC CTGGGGCCTG ACAACTCGAA CTATTGGTGT TATGACCATG GTTCATGGGG ACAACATGGG TTTAGTATTA CCACCCCGTG TAGCATGTGT TCAGGTGGTG ATTATTCCTT GTGGCATTAC CAATGCACTT TCTGAAGAAG ACAAAGAAGC GCTGATTGCA AAATGCAATG ATTATCGAAG GCGATTACTC AGTGTTAACA TCCGCGTTAG AGCTGATTTA CGAGATAATT ATTCTCCAGG TTGGAAATTC AATCACTGGG AGCTCAAGGG AGTTCCCATT AGACTTGAAG TTGGGCCACG TGATATGAAG AGCTGTCAGT TTGTAGCCGT CAGACGAGAT ACTGGAGAAA AGCTGACAGT TGCTGAAAAT GAGGCAGAGA CTAAACTTCA AGCTATTTTG GAAGACATCC AGGTCACCCT TTTCACAAGG GCTTCTGAAG ACCTTAAGAC TCATATGGTT GTGGCTAATA CAATGGAAGA CTTTCAGAAG ATACTAGATT CTGGAAAGAT TGTTCAGATT CCATTCTGTG GGGAAATTGA CTGTGAGGAC TGGATCAAAA AGACCACTGC CAGGGATCAA GATCTTGAAC CTGGTGCTCC ATCCATGGGA GCTAAAAGCC TTTGCATCCC CTTCAAACCA CTCTGTGAAC TGCAGCCTGG AGCCAAATGT GTCTGTGGCA AGAACCCTGC CAAGTACTAC ACCTTATTTG GTCGCAGCTA CGGATCCGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGTGAG GGCCCGATAT CTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCCTGGA AACGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC CGCGCTATGA CGGCAATAAA AAGACAGAAT AAAACGCACG GGTGTTGGGT CGTTTGTTCA TAAACGCGGG GTTCGGTCCC AGGGCTGGCA CTCTGTCGAT ACCCCACCGA GACCCCATTG GGGCCAATAC GCCCGCGTTT CTTCCTTTTC CCCACCCCAC CCCCCAAGTT CGGGTGAAGG CCCAGGGCTC GCAGCCAACG TCGGGGCGGC AGGCCCTGCC ATAGCAGATC TGCGCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGCAT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GGGGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGTC GACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATCTGC TCCCTGCTTG TGTGTTGGAG GTCGCTGAGT AGTGCGCGAG CAAAATTTAA GCTACAACAA GGCAAGGCTT GACCGACAAT TGCATGAAGA ATCTGCTTAG G
