NM_001357.5;DDX9; LKP; NDH2; NDHII; RHA
100μg liquid plasmid(Instantly):$134
| name | pLV2-TRE3GS-DHX9(human)-3×FLAG-TetOne-Blast | another name | apLV2-TRE3GS-DHX9(human)-3×FLAG-TetOne-Blast |
| Insert Size (bp) | 3810bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Blast | Fluorescent labeling | |
| Promoter | TRE3GS | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTACTAAGT TTGTAGTACA TATTTAACAA ATACAATTTC TTTAAAAACT ACTAAGTTTG TAGTACATAT TTAACAAATA CAATTTCTTT AAAATGAAAA TAATTCAGAG GAATCACAGG TTTAGAGTAA ATGAAACCAC TAGGTAATTG GCAGTGGTAA TAGGGTATGG GGTGGGAAGT TTGGGATGAT TTTGGTTAGC TTGAGTTATC CAGTTGATCC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT GATCTCCCGA TCCGTCGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCT AGCTAGAGGT CGACGGTAAA ACGCTAGCAA TGTCAAGGCC TCTCACTCTC TGATATTCAT TTCTTTGCAA GTTATAAATA CTGAATAATA AGATGACATG AACTACTACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGGCA AAAAGCAGCT GCTTATATGC AGGATCTGAG GGCTCGCCAC TCCCCAGTCC CGCCCAGGCC ACGCCTCCCT GGAAAGTCCC CAGCGGAAAG TCCCTTGTAG CAAGCTCGAT ATCAGCAGTT CTTGAAGTAC TCCGGATGCA GCTCTCGGGC CACGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AACACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCTGGTG TTCTCTCCTT TATTGGCCTC TTCTACCTTA TCTGGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGACCCCT GGCCCTGGTG TGTAGTTCTG CTAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCT CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACTGCAGAAT TAATTCCAGG CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG CGAAGGCGAA GACGCGGAAG AGGCCGCAGA GCCGGCAGCA GGCCGCGGGA AGGAAGGTCC GCTGGATTGA GGGCCGAAGG GACGTAGCAG AAGGACGTCC CGCGCAGAAT CCAGGTGGCA ACACAGGCGA GCAGCCATGG AAAGGACGTC AGCTTCCCCG ACAACACCAC GGAATTGTCA GTGCCCAACA GCCGAGCCCC TGTCCAGCAG CGGGCAAGGC AGGCGGCGAT GAGTTCCGCC GTGGCAATAG GGAGGGGGAA AGCGAAAGTC CCGGAAAGGA GCTGACAGGT GGTGGCAATG CCCCAACCAG TGGGGGTTGC GTCAGCAAAC ACAGTGCACA CCACGCCACG TTGCCTGACA ACGGGCCACA ACTCCTCATA AAGAGACAGC AACCAGGATT TATACAAGGA GGAGAAAATG AAAGCCATAC GGGAAGCAAT AGCATGATAC AAAGGCATTA AAGCAGCGTA TCCACATAGC GTAAAAGGAG CAACATAGTT AAGAATACCA GTCAATCTTT CACAAATTTT GTAATCCAGA GGTTGATTGT TCCAGACGCG TTAGCCCTCC CACACATAAC CAGAGGGCAG CAATTCACGA ATCCCAACTG CCGTCGGCTG TCCATCACTG TCCTTCACTA TGGCTTTGAT CCCAGGATGC AGATCGAGAA GCACCTGTCG GCACCGTCCG CAGGGGCTCA AGATGCCCCT GTTCTCATTT CCGATCGCGA CGATACAAGT CAGGTTGCCA GCTGCCGCAG CAGCAGCAGT GCCCAGCACC ACGAGTTCTG CACAAGGTCC CCCAGTAAAA TGATATACAT TGACACCAGT GAAGATGCGG CCGTCGCTAG AGAGAGCTGC GCTGGCGACG CTGTAGTCTT CAGAGATGGG GATGCTGTTG ATTGTAGCCG TTGCTCTTTC AATGAGGGTG GATTCTTCTT GAGACAAAGG CTTGGCCATG GTGGCGGTCG CGTTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCGCG CGTTTACCCG GGGAGCATGT CAAGGTCAAA ATCGTCAAGA GCGTCAGCAG GCAGCATATC AAGGTCAAAG TCGTCAAGGG CATCGGCTGG GAGCATGTCT AAGTCAAAAT CGTCAAGGGC GTCGGTCGGC CCGCCGCTTT CGCACTTTAG CTGTTTCTCC AGGCCACATA TGATTAGTTC CAGGCCGAAA AGGAAGGCAG GTTCGGCTCC CTGCCGGTCG AACAGCTCAA TTGCTTGTTT CAGAAGTGGG GGCATAGAAT CGGTGGTAGG TGTCTCTCTT TCCTCTTTTG CTACTTGATG CTCCTGTTCC TCCAATACGC AGCCCAGTGT AAAGTGGCCC ACGGCGGACA GAGCGTACAG TGCGTTCTCC AGGGAGAAGC CTTGCTGACA CAGGAACGCG AGCTGATTTT CCAGGGTTTC GTACTGTTTC TCTGTTGGGC GGGTGCCGAG ATGCACTTTA GCCCCGTCGC GATGTGAGAG GAGAGCACAG CGGTATGACT TGGCGTTGTT CCGCAGAAAG TCTTGCCATG ACTCGCCTTC CAGGGGGCAG GAGTGGGTAT GATGCCTGTC CAGCATCTCG ATTGGCAGGG CATCGAGCAG GGCCCGCTTG TTCTTCACGT GCCAGTACAG GGTAGGCTGC TCAACTCCCA GCTTTTGAGC GAGTTTCCTT GTCGTCAGGC CTTCGATACC GACTCCATTG AGTAATTCCA GAGCAGAGTT TATGACTTTG CTCTTGTCCA GTCTAGACAT GGTAATTCGA TGATCCCCCT GGGGAGAGAG GTCGGTGATT CGGTCAACGA GGGAGCCGAC TGCCGACGTG CGCTCCGGAG GCTTGCAGAA TGCGGAACAC CGCGCGGGCA GGAACAGGGC CCACACTACC GCCCCACACC CCGCCTCCCG CACCGCCCCT TCCCGGCCGC TGCTCTCGGC GCGCCCTGCT GAGCAGCCGC TATTGGCCAC AGCCCATCGC GGTCGGCGCG CTGCCATTGC TCCCTGGCGC TGTCCGTCTG CGAGGGTACT AGTGAGACGT GCGGCTTCCG TTTGTCACGT CCGGCACGCC GCGAACCGCA AGGAACCTTC CCGACTTAGG GGCGGAGCAG GAAGCGTCGC CGGGGGGCCC ACAAGGGTAG CGGCGAAGAT CCGGGTGACG CTGCGAACGG ACGTGAAGAA TGTGCGAGAC CCAGGGTCGG CGCCGCTGCG TTTCCCGGAA CCACGCCCAG AGCAGCCGCG TCCCTGCGCA AACCCAGGGC TGCCTTGGAA AAGGCGCAAC CCCAACCCCG TGGAATTATC ACCTCGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGA AGAGTTTACT CCCTATCAGT GATAGAGAAC GTATGCAGAC TTTACTCCCT ATCAGTGATA GAGAACGTAT AAGGAGTTTA CTCCCTATCA GTGATAGAGA ACGTATGACC AGTTTACTCC CTATCAGTGA TAGAGAACGT ATCTACAGTT TACTCCCTAT CAGTGATAGA GAACGTATAT CCAGTTTACT CCCTATCAGT GATAGAGAAC GTATAAGCTT TGCTTATGTA AACCAGGGCG CCTATAAAAG AGTGCTGATT TTTTGAGTAA ACTTCAATTC CACAACACTT TTGTCTTATA CCAACTTTCC GTACCACTTC CTACCCTCGT AAAGAATTCG CCACCATGGG TGACGTTAAA AATTTTCTGT ATGCCTGGTG TGGCAAAAGG AAGATGACCC CATCCTATGA AATTAGAGCA GTGGGGAACA AAAACAGGCA GAAATTCATG TGTGAGGTTC AGGTGGAAGG TTATAATTAC ACTGGCATGG GAAATTCCAC CAATAAAAAA GATGCACAAA GCAATGCTGC CAGAGACTTT GTTAACTATT TGGTTCGAAT AAATGAAATA AAGAGTGAAG AAGTTCCAGC TTTTGGGGTA GCATCTCCGC CCCCACTTAC TGATACTCCT GACACTACAG CAAATGCTGA AGGAGATTTA CCAACAACCA TGGGAGGACC TCTTCCTCCA CATCTGGCTC TCAAAGCAGA AAATAATTCT GAGGTAGGGG CCTCTGGCTA TGGTGTTCCT GGGCCCACCT GGGACCGAGG AGCCAACTTG AAGGATTACT ACTCAAGAAA GGAAGAACAA GAAGTGCAAG CGACTCTAGA ATCAGAAGAA GTGGATTTAA ATGCTGGGCT TCATGGAAAC TGGACCTTGG AAAATGCTAA AGCTCGTCTA AACCAATATT TTCAGAAAGA AAAGATCCAA GGAGAATATA AGTACACCCA AGTGGGTCCT GATCACAACA GGAGCTTTAT TGCAGAAATG ACCATTTATA TCAAGCAGCT GGGCAGAAGG ATTTTTGCAC GAGAACATGG ATCAAATAAG AAATTGGCAG CACAGTCCTG TGCCCTGTCA CTTGTCAGAC AACTGTACCA TCTTGGAGTG GTTGAAGCTT ACTCCGGACT TACAAAGAAG AAGGAAGGAG AGACAGTGGA GCCTTACAAA GTAAACCTCT CTCAAGATTT AGAGCATCAG CTGCAAAACA TCATTCAAGA GCTAAATCTT GAGATTTTGC CCCCGCCTGA AGATCCTTCT GTGCCAGTTG CACTCAACAT TGGCAAATTG GCTCAGTTCG AACCATCTCA GCGACAAAAC CAAGTGGGTG TGGTTCCTTG GTCACCTCCA CAATCCAACT GGAATCCTTG GACTAGTAGC AACATTGATG AGGGGCCTCT GGCTTTTGCT ACTCCAGAGC AAATAAGCAT GGACCTCAAG AATGAATTGA TGTACCAGTT GGAACAGGAT CATGATTTGC AAGCAATCTT GCAGGAGAGA GAGTTACTGC CTGTGAAGAA ATTTGAAAGT GAGATTCTGG AAGCAATCAG CCAAAATTCA GTTGTCATTA TTAGAGGGGC TACTGGATGT GGGAAAACCA CACAGGTTCC CCAGTTCATT CTAGATGACT TTATCCAGAA TGACCGAGCA GCAGAGTGTA ACATCGTAGT AACTCAGCCC AGAAGAATCA GTGCGGTTTC TGTGGCAGAG CGAGTTGCAT TTGAAAGAGG AGAAGAGCCT GGAAAAAGCT GTGGCTACAG CGTTCGATTT GAGTCTATAC TTCCTCGTCC TCATGCCAGT ATAATGTTTT GTACTGTAGG TGTGCTCCTG AGAAAATTAG AAGCAGGCAT TCGAGGAATC AGTCATGTAA TTGTAGATGA AATACATGAA AGAGATATTA ATACTGACTT CCTTTTGGTA GTACTGCGTG ATGTTGTTCA GGCTTATCCT GAAGTTCGCA TTGTTCTTAT GTCTGCTACT ATTGATACCA GCATGTTTTG TGAATATTTC TTCAATTGCC CCATCATTGA AGTTTATGGG AGGACTTACC CAGTTCAAGA ATATTTTCTG GAAGACTGCA TTCAGATGAC CCACTTTGTT CCTCCACCAA AAGACAAAAA GAAGAAGGAT AAGGATGATG ATGGTGGTGA GGATGATGAT GCAAATTGCA ACTTGATCTG TGGTGATGAA TATGGTCCAG AAACAAGGTT GAGCATGTCT CAATTGAACG AAAAGGAAAC TCCTTTTGAA CTCATCGAGG CTCTACTTAA GTACATTGAA ACCCTTAATG TTCCTGGAGC TGTGTTGGTT TTTTTGCCTG GCTGGAATCT GATTTATACT ATGCAGAAGC ATTTGGAAAT GAATCCACAT TTTGGAAGCC ATCGGTATCA GATTCTACCC CTGCATTCTC AGATTCCTCG AGAGGAACAG CGCAAAGTGT TTGATCCAGT ACCAGTTGGA GTAACCAAGG TTATTTTGTC CACAAATATT GCTGAAACAA GCATTACCAT AAACGATGTT GTTTATGTCA TTGACTCCTG CAAGCAGAAA GTGAAACTCT TCACTGCTCA CAACAATATG ACCAACTATG CTACCGTATG GGCATCAAAA ACAAACCTTG AGCAACGGAA AGGGCGAGCT GGCCGAGTAC GGCCTGGATT CTGCTTTCAC CTGTGCAGCC GAGCTCGTTT TGAGAGACTT GAAACCCACA TGACACCAGA GATGTTCCGA ACACCATTGC ATGAAATTGC TCTTAGCATA AAACTTCTGC GTCTAGGAGG AATTGGCCAA TTTCTGGCCA AAGCAATTGA ACCTCCCCCT TTGGATGCTG TGATTGAAGC AGAACACACT CTTAGAGAGC TTGATGCATT AGATGCCAAT GATGAGTTGA CTCCTTTGGG ACGAATCCTG GCTAAACTCC CCATTGAGCC TCGTTTTGGC AAAATGATGA TAATGGGGTG TATTTTCTAC GTGGGAGATG CTATCTGTAC CATTGCTGCT GCTACCTGCT TTCCAGAGCC TTTCATCAAT GAAGGAAAGC GGCTGGGCTA TATCCATCGA AATTTTGCTG GAAACAGATT TTCTGATCAC GTAGCCCTTT TATCAGTATT CCAAGCCTGG GATGATGCTA GAATGGGTGG AGAAGAAGCA GAGATACGTT TTTGTGAGCA CAAAAGACTT AATATGGCTA CACTAAGAAT GACTTGGGAA GCCAAAGTTC AGCTCAAAGA GATTTTGATT AATTCTGGGT TTCCAGAAGA TTGTTTGTTG ACACAAGTGT TTACTAACAC TGGACCAGAT AATAATTTGG ATGTTGTTAT CTCCCTCCTG GCCTTTGGTG TGTACCCCAA TGTATGCTAT CATAAGGAAA AGAGGAAGAT TCTCACCACT GAAGGGCGTA ATGCACTTAT CCACAAATCA TCTGTTAATT GTCCTTTTAG TAGCCAAGAC ATGAAGTACC CATCTCCCTT CTTTGTATTT GGTGAAAAGA TTCGAACTCG AGCCATCTCT GCTAAAGGCA TGACTTTAGT CACCCCCCTG CAGTTGCTTC TCTTTGCCTC CAAGAAAGTC CAATCTGATG GGCAGATTGT GCTTGTAGAT GACTGGATTA AACTGCAAAT ATCTCATGAA GCTGCTGCCT GTATCACTGG TCTCCGGGCA GCCATGGAGG CTTTGGTTGT TGAAGTAACC AAACAACCTG CTATCATCAG CCAGTTGGAC CCCGTAAATG AACGTATGCT GAACATGATC CGTCAGATCT CTAGACCCTC AGCTGCTGGT ATCAACCTTA TGATTGGCAG TACACGGTAT GGAGATGGTC CACGTCCTCC CAAGATGGCC CGATACGACA ATGGAAGCGG ATATAGAAGG GGAGGTTCTA GTTACAGTGG TGGAGGCTAT GGCGGTGGCT ATAGCAGTGG AGGCTATGGT AGCGGAGGCT ATGGTGGCAG CGCCAACTCC TTTCGGGCAG GATATGGTGC AGGTGTTGGT GGAGGCTATA GAGGAGTTTC CCGAGGTGGC TTTAGAGGCA ACTCTGGAGG AGACTACAGA GGGCCTAGTG GAGGCTACAG AGGATCTGGG GGATTCCAGC GAGGAGGTGG TAGGGGGGCC TATGGAACTG GCTACTTTGG ACAGGGAAGA GGAGGTGGCG GCTATACCGG TGGCGGTGGA GGAAGCGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGTAGGATCC AGACCACCTC CCCTGCGAGC TAAGCTGGAC AGCCAATGAC GGGTAAGAGA GTGACATTTT TCACTAACCT AAGACAGGAG GGCCGTCAGA GCTACTGCCT AATCCAAAGA CGGGTAAAAG TGATAAAAAT GTATCACTCC AACCTAAGAC AGGCGCAGCT TCCGAGGGAT TTGAGATCCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCAAAA CTAGAATGCA GTGAAAAAAA TGCCTTATTT GTGAAATTTG TGATGCTATT GCCTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA AGTCGATAAA CTGGATCTCT GCTGTCCCTG TAATAAACCC GAAAATTTTG AATTTTTGTA ATTTGTTTTT GTAGTTCTTT AGTTTGTATG TCTGTTGCTA TTATGTCTAC TATTCTTTCC CCTGCACTGT ACCCCCCAAT CCCCCCTTTT CTTTTAAAGG CGATACCGTC GAGATCCGTT CACTAATCGA ATGGATCTGT CTCTGTCTCT CTCTCCACCT TCTTCTTCTA TTCCTTCGGG CCTGTCGGGT CCCCTCGGGG TTGGGAGGTG GGTCTGAAAC GATAATGGTG AATATCCCTG CCTAACTCTA TTCACTATAG AAAGTACAGC AAAAACTATT CTTAAACCTA CCAAGCCTCC TACTATCATT ATGAATAATT TTATATACCA CAGCCAATTT GTTATGTTAA ACCAATTCCA CAAACTTGCC CATTTATCTA ATTCCAATAA TTCTTGTTCA TTCTTTTCTT GCTGGTTTTG CGATTCTTCA ATTAAGGAGT GTATTAAGCT TGTGTAATTG TTAATTTCTC TGTCCCACTC CATCCAGGTC GTGTGATTCC AAATCTGTTC CAGAGATTTA TTACTCCAAC TAGCATTCCA AGGCACAGCA GTGGTGCAAA TGAGTTTTCC AGAGCAACCC CAAATCCCCA GGAGCTGTTG ATCCTTTAGG TATCTTTCCA CAGCCAGGAT TCTTGCCTGG AGCTGCTTGA TGCCCCAGAC TGTGAGTTGC AACAGATGCT GTTGCGCCTC AATAGCCCTC AGCAAATTGT TCTGCTGCTG CACTATACCA GACAATAATT GTCTGGCCTG TACCGTCAGC GTCATTGACG CTGCGCCCAT AGTGCTTCCT GCTGCTCCCA AGAACCCAAG GAACAAAGCT CCTATTCCCA CTGCTCTTTT TTCTCTCTGC ACCACTCTTC TCTTTGCCTT GGTGGGTGCT ACTCCTAATG GTTCAATTTT TACTACTTTA TATTTATATA ATTCACTTCT CCAATTGTCC CTCATATCTC CTCCTCCAGG TCTGAAGATC AGCGGCCGGC CGCTTGCTGT GCGGTGGTCT TACTTTTGTT TTGCTCTTCC TCTATCTTGT CTAAAGCTTC CTTGGTGTCT TTTATCTCTA TCCTTTGATG CACACAATAG AGGGTTGCTA CTGTATTATA TAATGATCTA AGTTCTTCTG ATCCTGTCTG AAGGGATGGT TGTAGCTGTC CCAGTATTTG TCTACAGCCT TCTGATGTTT CTAACAGGCC AGGATTAACT GCGAATCGTT CTAGCTCCCT GCTTGCCCAT ACTATATGTT TTAATTTATA TTTTTTCTTT CCCCCTGGCC TTAACCGAAT TTTTTCCCAT CGCGATCTAA TTCTCCCCCG CTTAATACTG ACGCTCTCGC ACCCATCTCT CTCCTTCTAG CCTCCGCTAG TCAAAATTTT TGGCGTACTC ACCAGTCGCC GCCCCTCGCC TCTTGCCGTG CGCGCTTCAG CAAGCCGAGT CCTGCGTCGA GAGAGCTCCT CTGGTTTCCC TTTCGCTTTC AAGTCCCTGT TCGGGCGCCA CTGCTAGAGA TTTTCCACAC TGACTAAAAG GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGG CAAAAAGCAG CTGCTTATAT GCAGGATCTG AGGGCTCGCC ACTCCCCAGT CCCGCCCAGG CCACGCCTCC CTGGAAAGTC CCCAGCGGAA AGTCCCTTGT AGCAAGCTCG ATATCAGCAG TTCTTGAAGT ACTCCGGATG CAGCTCTCGG GCCACGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAACACTTC TCTCTCCGGG TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCTGG TGTTCTCTCC TTTATTGGCC TCTTCTACCT TATCTGGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA TATCTGACCC CTGGCCCTGG TGTGTAGTTC TGCTAATCAG GGAAGTAGCC TTGTGTGTGG TAGATCCACA GATCAAGGAT ATCTTGTCTT CTTTGGGAGT GAATTAGCCC TTCCA
