PiggyBac-CMV-SCN10A(human)-Puro
$142.00
In stock
SKU
PL39670
NM_006514.4;FEPS2; Nav1.8; PN3; SNS
100μg liquid plasmid(Instantly):$163
| name | PiggyBac-CMV-SCN10A(human)-Puro | another name | aPiggyBac-CMV-SCN10A(human)-Puro |
| Insert Size (bp) | 5871bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG ACACCTGGGG GGATACGGGG AAAAGGCCTC CAAGGCCTAC TAGTATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGATT CTAGAGCTAG CGAATTCGCC ACCATGGAAT TCCCCATTGG ATCCCTCGAA ACTAACAACT TCCGTCGCTT TACTCCGGAG TCACTGGTGG AGATAGAGAA GCAAATTGCT GCCAAGCAGG GAACAAAGAA AGCCAGAGAG AAGCATAGGG AGCAGAAGGA CCAAGAAGAG AAGCCTCGGC CCCAGCTGGA CTTGAAAGCC TGCAACCAGC TGCCCAAGTT CTATGGTGAG CTCCCAGCAG AACTGATCGG GGAGCCCCTG GAGGATCTAG ATCCGTTCTA CAGCACACAC CGGACATTTA TGGTGCTGAA CAAAGGGAGG ACCATTTCCC GGTTTAGTGC CACTCGGGCC CTGTGGCTAT TCAGTCCTTT CAACCTGATC AGAAGAACGG CCATCAAAGT GTCTGTCCAC TCGTGGTTCA GTTTATTTAT TACGGTCACT ATTTTGGTTA ATTGTGTGTG CATGACCCGA ACTGACCTTC CAGAGAAAAT TGAATATGTC TTCACTGTCA TTTACACCTT TGAAGCCTTG ATAAAGATAC TGGCAAGAGG ATTTTGTCTA AATGAGTTCA CGTACCTGAG AGATCCTTGG AACTGGCTGG ATTTTAGCGT CATTACCCTG GCATATGTTG GCACAGCAAT AGATCTCCGT GGGATCTCAG GCCTGCGGAC ATTCAGAGTT CTTAGAGCAT TAAAAACAGT TTCTGTGATC CCAGGCCTGA AGGTCATTGT GGGGGCCCTG ATTCACTCAG TGAAGAAACT GGCTGATGTG ACCATCCTCA CCATCTTCTG CCTAAGTGTT TTTGCCTTGG TGGGGCTGCA ACTCTTCAAG GGCAACCTCA AAAATAAATG TGTCAAGAAT GACATGGCTG TCAATGAGAC AACCAACTAC TCATCTCACA GAAAACCAGA TATCTACATA AATAAGCGAG GCACTTCTGA CCCCTTACTG TGTGGCAATG GATCTGACTC AGGCCACTGC CCTGATGGTT ATATCTGCCT TAAAACTTCT GACAACCCGG ATTTTAACTA CACCAGCTTT GATTCCTTTG CTTGGGCTTT CCTCTCACTG TTCCGCCTCA TGACACAGGA TTCCTGGGAA CGCCTCTACC AGCAGACCCT GAGGACTTCT GGGAAAATCT ATATGATCTT TTTTGTGCTC GTAATCTTCC TGGGATCTTT CTACCTGGTC AACTTGATCT TGGCTGTAGT CACCATGGCG TATGAGGAGC AGAACCAGGC AACCACTGAT GAAATTGAAG CAAAGGAGAA GAAGTTCCAG GAGGCCCTCG AGATGCTCCG GAAGGAGCAG GAGGTGCTAG CAGCACTAGG GATTGACACA ACCTCTCTCC ACTCCCACAA TGGATCACCT TTAACCTCCA AAAATGCCAG TGAGAGAAGG CATAGAATAA AGCCAAGAGT GTCAGAGGGC TCCACAGAAG ACAACAAATC ACCCCGCTCT GATCCTTACA ACCAGCGCAG GATGTCTTTT CTAGGCCTCG CCTCTGGAAA ACGCCGGGCT AGTCATGGCA GTGTGTTCCA TTTCCGGTCC CCTGGCCGAG ATATCTCACT CCCTGAGGGA GTCACAGATG ATGGAGTCTT TCCTGGAGAC CACGAAAGCC ATCGGGGCTC TCTGCTGCTG GGTGGGGGTG CTGGCCAGCA AGGCCCCCTC CCTAGAAGCC CTCTTCCTCA ACCCAGCAAC CCTGACTCCA GGCATGGAGA AGATGAACAC CAACCGCCGC CCACTAGTGA GCTTGCCCCT GGAGCTGTCG ATGTCTCGGC ATTCGATGCA GGACAAAAGA AGACTTTCTT GTCAGCAGAA TACTTAGATG AACCTTTCCG GGCCCAAAGG GCAATGAGTG TTGTCAGTAT CATAACCTCC GTCCTTGAGG AACTCGAGGA GTCTGAACAG AAGTGCCCAC CCTGCTTGAC CAGCTTGTCT CAGAAGTATC TGATCTGGGA TTGCTGCCCC ATGTGGGTGA AGCTCAAGAC AATTCTCTTT GGGCTTGTGA CGGATCCCTT TGCAGAGCTC ACCATCACCT TGTGCATCGT GGTGAACACC ATCTTCATGG CCATGGAGCA CCATGGCATG AGCCCTACCT TCGAAGCCAT GCTCCAGATA GGCAACATCG TCTTTACCAT ATTTTTTACT GCTGAAATGG TCTTCAAAAT CATTGCCTTC GACCCATACT ATTATTTCCA GAAGAAGTGG AATATCTTTG ACTGCATCAT CGTCACTGTG AGTCTGCTAG AGCTGGGCGT GGCCAAGAAG GGAAGCCTGT CTGTGCTGCG GAGCTTCCGC TTGCTGCGCG TATTCAAGCT GGCCAAATCC TGGCCCACCT TAAACACACT CATCAAGATC ATCGGAAACT CAGTGGGGGC ACTGGGGAAC CTCACCATCA TCCTGGCCAT CATTGTCTTT GTCTTTGCTC TGGTTGGCAA GCAGCTCCTA GGGGAAAACT ACCGTAACAA CCGAAAAAAT ATCTCCGCGC CCCATGAAGA CTGGCCCCGC TGGCACATGC ACGACTTCTT CCACTCTTTC CTCATTGTCT TCCGTATCCT CTGTGGAGAG TGGATTGAGA ACATGTGGGC CTGCATGGAA GTTGGCCAAA AATCCATATG CCTCATCCTT TTCTTGACGG TGATGGTGCT AGGGAACCTG GTGGTGCTTA ACCTGTTCAT CGCCCTGCTA TTGAACTCTT TCAGTGCTGA CAACCTCACA GCCCCGGAGG ACGATGGGGA GGTGAACAAC CTGCAGGTGG CCCTGGCACG GATCCAGGTC TTTGGCCATC GTACCAAACA GGCTCTTTGC AGCTTCTTCA GCAGGTCCTG CCCATTCCCC CAGCCCAAGG CAGAGCCTGA GCTGGTGGTG AAACTCCCAC TCTCCAGCTC CAAGGCTGAG AACCACATTG CTGCCAACAC TGCCAGGGGG AGCTCTGGAG GGCTCCAAGC TCCCAGAGGC CCCAGGGATG AGCACAGTGA CTTCATCGCT AATCCGACTG TGTGGGTCTC TGTGCCCATT GCTGAGGGTG AATCTGATCT TGATGACTTG GAGGATGATG GTGGGGAAGA TGCTCAGAGC TTCCAGCAGG AAGTGATCCC CAAAGGACAG CAGGAGCAGC TGCAGCAAGT CGAGAGGTGT GGGGACCACC TGACACCCAG GAGCCCAGGC ACTGGAACAT CTTCTGAGGA CCTGGCTCCA TCCCTGGGTG AGACGTGGAA AGATGAGTCT GTTCCTCAGG TCCCTGCTGA GGGAGTGGAC GACACAAGCT CCTCTGAGGG CAGCACGGTG GACTGCCTAG ATCCTGAGGA AATCCTGAGG AAGATCCCTG AGCTGGCAGA TGACCTGGAA GAACCAGATG ACTGCTTCAC AGAAGGATGC ATTCGCCACT GTCCCTGCTG CAAACTGGAT ACCACCAAGA GTCCATGGGA TGTGGGCTGG CAGGTGCGCA AGACTTGCTA CCGTATCGTG GAGCACAGCT GGTTTGAGAG CTTCATCATC TTCATGATCC TGCTCAGCAG TGGATCTCTG GCCTTTGAAG ACTATTACCT GGACCAGAAG CCCACGGTGA AAGCTTTGCT GGAGTACACT GACAGGGTCT TCACCTTTAT CTTTGTGTTC GAGATGCTGC TTAAGTGGGT GGCCTATGGC TTCAAAAAGT ACTTCACCAA TGCCTGGTGC TGGCTGGACT TCCTCATTGT GAATATCTCA CTGATAAGTC TCACAGCGAA GATTCTGGAA TATTCTGAAG TGGCTCCCAT CAAAGCCCTT CGAACCCTTC GCGCTCTGCG GCCACTGCGG GCTCTTTCTC GATTTGAAGG CATGCGGGTG GTGGTGGATG CCCTGGTGGG CGCCATCCCA TCCATCATGA ATGTCCTCCT CGTCTGCCTC ATCTTCTGGC TCATCTTCAG CATCATGGGT GTGAACCTCT TCGCAGGGAA GTTTTGGAGG TGCATCAACT ATACCGATGG AGAGTTTTCC CTTGTACCTT TGTCGATTGT GAATAACAAG TCTGACTGCA AGATTCAAAA CTCCACTGGC AGCTTCTTCT GGGTCAATGT GAAAGTCAAC TTTGATAATG TTGCAATGGG TTACCTTGCA CTTCTGCAGG TGGCAACCTT TAAAGGCTGG ATGGACATTA TGTATGCAGC TGTTGATTCC CGGGAGGTCA ACATGCAACC CAAGTGGGAG GACAACGTGT ACATGTATTT GTACTTTGTC ATCTTCATCA TTTTTGGAGG CTTCTTCACA CTGAATCTCT TTGTTGGGGT CATAATTGAC AACTTCAATC AACAGAAAAA AAAGTTAGGG GGCCAGGACA TCTTCATGAC AGAGGAGCAG AAGAAATACT ACAATGCCAT GAAGAAGTTG GGCTCCAAGA AGCCCCAGAA GCCCATCCCA CGGCCCCTGA ACAAGTTCCA GGGTTTTGTC TTTGACATCG TGACCAGACA AGCTTTTGAC ATCACCATCA TGGTCCTCAT CTGCCTCAAC ATGATCACCA TGATGGTGGA GACTGATGAC CAAAGTGAAG AAAAGACGAA AATTCTGGGC AAAATCAACC AGTTCTTTGT GGCCGTCTTC ACAGGCGAAT GTGTCATGAA GATGTTCGCT TTGAGGCAGT ACTACTTCAC AAATGGCTGG AATGTGTTTG ACTTCATTGT GGTGGTTCTC TCCATTGCGA GCCTGATTTT TTCTGCAATT CTTAAGTCAC TTCAAAGTTA CTTCTCCCCA ACGCTCTTCA GAGTCATCCG CCTGGCCCGA ATTGGCCGCA TCCTCAGACT GATCCGAGCG GCCAAGGGGA TCCGCACACT GCTCTTTGCC CTCATGATGT CCCTGCCTGC CCTCTTCAAC ATCGGGCTGT TGCTATTCCT TGTCATGTTC ATCTACTCTA TCTTCGGTAT GTCCAGCTTT CCCCATGTGA GGTGGGAGGC TGGCATCGAC GACATGTTCA ACTTCCAGAC CTTCGCCAAC AGCATGCTGT GCCTCTTCCA GATTACCACG TCGGCCGGCT GGGATGGCCT CCTCAGCCCC ATCCTCAACA CAGGGCCCCC CTACTGTGAC CCCAATCTGC CCAACAGCAA TGGCACCAGA GGGGACTGTG GGAGCCCAGC CGTAGGCATC ATCTTCTTCA CCACCTACAT CATCATCTCC TTCCTCATCA TGGTCAACAT GTACATTGCA GTGATTCTGG AGAACTTCAA TGTGGCCACG GAGGAGAGCA CTGAGCCCCT GAGTGAGGAC GACTTTGACA TGTTCTATGA GACCTGGGAG AAGTTTGACC CAGAGGCCAC TCAGTTTATT ACCTTTTCTG CTCTCTCGGA CTTTGCAGAC ACTCTCTCTG GTCCCCTGAG AATCCCAAAA CCCAATCGAA ATATACTGAT CCAGATGGAC CTGCCTTTGG TCCCTGGAGA TAAGATCCAC TGCTTGGACA TCCTTTTTGC TTTCACCAAG AATGTCCTAG GAGAATCCGG GGAGTTGGAT TCTCTGAAGG CAAATATGGA GGAGAAGTTT ATGGCAACTA ATCTTTCAAA ATCATCCTAT GAACCAATAG CAACCACTCT CCGATGGAAG CAAGAAGACA TTTCAGCCAC TGTCATTCAA AAGGCCTATC GGAGCTATGT GCTGCACCGC TCCATGGCAC TCTCTAACAC CCCATGTGTG CCCAGAGCTG AGGAGGAGGC TGCATCACTC CCAGATGAAG GTTTTGTTGC ATTCACAGCA AATGAAAATT GTGTACTCCC AGACAAATCT GAAACTGCTT CTGCCACATC ATTCCCACCG TCCTATGAGA GTGTCACTAG AGGCCTTAGT GATAGAGTCA ACATGAGGAC ATCTAGCTCA ATACAAAATG AAGATGAAGC CACCAGTATG GAGCTGATTG CCCCTGGGCC CTAGTAGGCG GCCGCGAAGG ATCTGCGATC GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGAACG GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG CTGAAGCTTC GAGGGGCTCG CATCTCTCCT TCACGCGCCC GCCGCCCTAC CTGAGGCCGC CATCCACGCC GGTTGAGTCG CGTTCTGCCG CCTCCCGCCT GTGGTGCCTC CTGAACTGCG TCCGCCGTCT AGGTAAGTTT AAAGCTCAGG TCGAGACCGG GCCTTTGTCC GGCGCTCCCT TGGAGCCTAC CTAGACTCAG CCGGCTCTCC ACGCTTTGCC TGACCCTGCT TGCTCAACTC TACGTCTTTG TTTCGTTTTC TGTTCTGCGC CGTTACAGAT CCAAGCTGTG ACCGGCGCCT ACGCTAGACG CCACCATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGAAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGAATTGA CTCAAATGAT GTCAATTAGT CTATCAGAAG CTATCTGGTC TCCCTTCCGG GGGACAAGAC ATCCCTGTTT AATATTTAAA CAGCAGTGTT CCCAAACTGG GTTCTTATAT CCCTTGCTCT GGTCAACCAG GTTGCAGGGT TTCCTGTCCT CACAGGAACG AAGTCCCTAA AGAAACAGTG GCAGCCAGGT TTAGCCCCGG AATTGACTGG ATTCCTTTTT TAGGGCCCAT TGGTATGGCT TTTTCCCCGT ATCCCCCCAG GTGTCTGCAG GCTCAAAGAG CAGCGAGAAG CGTTCAGAGG AAAGCGATCC CGTGCCACCT TCCCCGTGCC CGGGCTGTCC CCGCACGCTG CCGGCTCGGG GATGCGGGGG GAGCGCCGGA CCGGAGCGGA GCCCCGGGCG GCTCGCTGCT GCCCCCTAGC GGGGGAGGGA CGTAATTACA TCCCTGGGGG CTTTGGGGGG GGGCTGTCCC TGATATCTAT AACAAGAAAA TATATATATA ATAAGTTATC ACGTAAGTAG AACATGAAAT AACAATATAA TTATCGTATG AGTTAAATCT TAAAAGTCAC GTAAAAGATA ATCATGCGTC ATTTTGACTC ACGCGGTCGT TATAGTTCAA AATCAGTGAC ACTTACCGCA TTGACAAGCA CGCCTCACGG GAGCTCCAAG CGGCGACTGA GATGTCCTAA ATGCACAGCG ACGGATTCGC GCTATTTAGA AAGAGAGAGC AATATTTCAA GAATGCATGC GTCAATTTTA CGCAGACTAT CTTTCTAGGG TTAATCTAGC TGCATCAGGA TCATATCGTC GGGTCTTTTT TCCGGCTCAG TCATCGCCCA AGCTGGCGCT ATCTGGGCAT CGGGGAGGAA GAAGCCCGTG CCTTTTCCCG CGAGGTTGAA GCGGCATGGA AAGAGTTTGC CGAGGATGAC TGCTGCTGCA TTGACGTTGA GCGAAAACGC ACGTTTACCA TGATGATTCG GGAAGGTGTG GCCATGCACG CCTTTAACGG TGAACTGTTC GTTCAGGCCA CCTGGGATAC CAGTTCGTCG CGGCTTTTCC GGACACAGTT CCGGATGGTC AGCCCGAAGC GCATCAGCAA CCCGAACAAT ACCGGCGACA GCCGGAACTG CCGTGCCGGT GTGCAGATTA ATGACAGCGG TGCGGCGCTG GGATATTACG TCAGCGAGGA CGGGTATCCT GGCTGGATGC CGCAGAAATG GACATGGATA CCCCGTGAGT TACCCGGCGG GCGCGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC AT
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