pCMV-AGRN(human)-3×FLAG-Neo
$142.00
In stock
SKU
PL39612
NM_198576.4;AGRIN; CMS8; CMSPPD
100μg liquid plasmid(Instantly):$163
| name | pCMV-AGRN(human)-3×FLAG-Neo | another name | apCMV-AGRN(human)-3×FLAG-Neo |
| Insert Size (bp) | 6135bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCGCCACC ATGGCCGGCC GGTCCCACCC GGGCCCGCTG CGGCCGCTGC TGCCGCTCCT TGTGGTGGCC GCGTGCGTCC TGCCCGGAGC CGGCGGGACA TGCCCGGAGC GCGCGCTGGA GCGGCGCGAG GAGGAGGCGA ACGTGGTGCT CACCGGGACG GTGGAGGAGA TCCTCAACGT GGACCCGGTG CAGCACACGT ACTCCTGCAA GGTTCGGGTC TGGCGGTACT TGAAGGGCAA AGACCTGGTG GCCCGGGAGA GCCTGCTGGA CGGCGGCAAC AAGGTGGTGA TCAGCGGCTT TGGAGACCCC CTCATCTGTG ACAACCAGGT GTCCACTGGG GACACCAGGA TCTTCTTTGT GAACCCTGCA CCCCCATACC TGTGGCCAGC CCACAAGAAC GAGCTGATGC TCAACTCCAG CCTCATGCGG ATCACCCTGC GGAACCTGGA GGAGGTGGAG TTCTGTGTGG AAGATAAACC CGGGACCCAC TTCACTCCAG TGCCTCCGAC GCCTCCTGAT GCGTGCCGGG GAATGCTGTG CGGCTTCGGC GCCGTGTGCG AGCCCAACGC GGAGGGGCCG GGCCGGGCGT CCTGCGTCTG CAAGAAGAGC CCGTGCCCCA GCGTGGTGGC GCCTGTGTGT GGGTCGGACG CCTCCACCTA CAGCAACGAA TGCGAGCTGC AGCGGGCGCA GTGCAGCCAG CAGCGCCGCA TCCGCCTGCT CAGCCGCGGG CCGTGCGGCT CGCGGGACCC CTGCTCCAAC GTGACCTGCA GCTTCGGCAG CACCTGTGCG CGCTCGGCCG ACGGGCTGAC GGCCTCGTGC CTGTGCCCCG CGACCTGCCG TGGCGCCCCC GAGGGGACCG TCTGCGGCAG CGACGGCGCC GACTACCCCG GCGAGTGCCA GCTCCTGCGC CGCGCCTGCG CCCGCCAGGA GAATGTCTTC AAGAAGTTCG ACGGCCCTTG TGACCCCTGT CAGGGCGCCC TCCCTGACCC GAGCCGCAGC TGCCGTGTGA ACCCGCGCAC GCGGCGCCCT GAGATGCTCC TACGGCCCGA GAGCTGCCCT GCCCGGCAGG CGCCAGTGTG TGGGGACGAC GGAGTCACCT ACGAAAACGA CTGTGTCATG GGCCGATCGG GGGCCGCCCG GGGTCTCCTC CTGCAGAAAG TGCGCTCCGG CCAGTGCCAG GGTCGAGACC AGTGCCCGGA GCCCTGCCGG TTCAATGCCG TGTGCCTGTC CCGCCGTGGC CGTCCCCGCT GCTCCTGCGA CCGCGTCACC TGTGACGGGG CCTACAGGCC CGTGTGTGCC CAGGACGGGC GCACGTATGA CAGTGATTGC TGGCGGCAGC AGGCTGAGTG CCGGCAGCAG CGTGCCATCC CCAGCAAGCA CCAGGGCCCG TGTGACCAGG CCCCGTCCCC ATGCCTCGGG GTGCAGTGTG CATTTGGGGC GACGTGTGCT GTGAAGAACG GGCAGGCAGC GTGTGAATGC CTGCAGGCGT GCTCGAGCCT CTACGATCCT GTGTGCGGCA GCGACGGCGT CACATACGGC AGCGCGTGCG AGCTGGAGGC CACGGCCTGT ACCCTCGGGC GGGAGATCCA GGTGGCGCGC AAAGGACCCT GTGACCGCTG CGGGCAGTGC CGCTTTGGAG CCCTGTGCGA GGCCGAGACC GGGCGCTGCG TGTGCCCCTC TGAATGCGTG GCTTTGGCCC AGCCCGTGTG TGGCTCCGAC GGGCACACGT ACCCCAGCGA GTGCATGCTG CACGTGCACG CCTGCACACA CCAGATCAGC CTGCACGTGG CCTCAGCTGG ACCCTGTGAG ACCTGTGGAG ATGCCGTGTG TGCTTTTGGG GCTGTGTGCT CCGCAGGGCA GTGTGTGTGT CCCCGGTGTG AGCACCCCCC GCCCGGCCCC GTGTGTGGCA GCGACGGTGT CACCTACGGC AGTGCCTGCG AGCTACGGGA AGCCGCCTGC CTCCAGCAGA CACAGATCGA GGAGGCCCGG GCAGGGCCGT GCGAGCAGGC CGAGTGCGGT TCCGGAGGCT CTGGCTCTGG GGAGGACGGT GACTGTGAGC AGGAGCTGTG CCGGCAGCGC GGTGGCATCT GGGACGAGGA CTCGGAGGAC GGGCCGTGTG TCTGTGACTT CAGCTGCCAG AGTGTCCCAG GCAGCCCGGT GTGCGGCTCA GATGGGGTCA CCTACAGCAC CGAGTGTGAG CTGAAGAAGG CCAGGTGTGA GTCACAGCGA GGGCTCTACG TAGCGGCCCA GGGAGCCTGC CGAGGCCCCA CCTTCGCCCC GCTGCCGCCT GTGGCCCCCT TACACTGTGC CCAGACGCCC TACGGCTGCT GCCAGGACAA TATCACCGCA GCCCGGGGCG TGGGCCTGGC TGGCTGCCCC AGTGCCTGCC AGTGCAACCC CCATGGCTCT TACGGCGGCA CCTGTGACCC AGCCACAGGC CAGTGCTCCT GCCGCCCAGG TGTGGGGGGC CTCAGGTGTG ACCGCTGTGA GCCTGGCTTC TGGAACTTTC GAGGCATCGT CACCGATGGC CGGAGTGGCT GTACACCCTG CAGCTGTGAT CCCCAAGGCG CCGTGCGGGA TGACTGTGAG CAGATGACGG GGCTGTGCTC GTGTAAGCCC GGGGTGGCTG GACCCAAGTG TGGGCAGTGT CCAGACGGCC GTGCCCTGGG CCCCGCGGGC TGTGAAGCTG ACGCTTCTGC GCCTGCGACC TGTGCGGAGA TGCGCTGTGA GTTCGGTGCG CGGTGCGTGG AGGAGTCTGG CTCAGCCCAC TGTGTCTGCC CGATGCTCAC CTGTCCAGAG GCCAACGCTA CCAAGGTCTG TGGGTCAGAT GGAGTCACAT ACGGCAACGA GTGTCAGCTG AAGACCATCG CCTGCCGCCA GGGCCTGCAA ATCTCTATCC AGAGCCTGGG CCCGTGCCAG GAGGCTGTTG CTCCCAGCAC TCACCCGACA TCTGCCTCCG TGACTGTGAC CACCCCAGGG CTCCTCCTGA GCCAGGCACT GCCGGCCCCC CCCGGCGCCC TCCCCCTGGC TCCCAGCAGT ACCGCACACA GCCAGACCAC CCCTCCGCCC TCATCACGAC CTCGGACCAC TGCCAGCGTC CCCAGGACCA CCGTGTGGCC CGTGCTGACG GTGCCCCCCA CGGCACCCTC CCCTGCACCC AGCCTGGTGG CGTCCGCCTT TGGTGAATCT GGCAGCACTG ATGGAAGCAG CGATGAGGAA CTGAGCGGGG ACCAGGAGGC CAGTGGGGGT GGCTCTGGGG GGCTCGAGCC CTTGGAGGGC AGCAGCGTGG CCACCCCTGG GCCACCTGTC GAGAGGGCTT CCTGCTACAA CTCCGCGTTG GGCTGCTGCT CTGATGGGAA GACGCCCTCG CTGGACGCAG AGGGCTCCAA CTGCCCCGCC ACCAAGGTGT TCCAGGGCGT CCTGGAGCTG GAGGGCGTCG AGGGCCAGGA GCTGTTCTAC ACGCCCGAGA TGGCTGACCC CAAGTCAGAA CTGTTCGGGG AGACAGCCAG GAGCATTGAG AGCACCCTGG ACGACCTCTT CCGGAATTCA GACGTCAAGA AGGATTTTCG GAGTGTCCGC TTGCGGGACC TGGGGCCCGG CAAATCCGTC CGCGCCATTG TGGATGTGCA CTTTGACCCC ACCACAGCCT TCAGGGCACC CGACGTGGCC CGGGCCCTGC TCCGGCAGAT CCAGGTGTCC AGGCGCCGGT CCTTGGGGGT GAGGCGGCCG CTGCAGGAGC ACGTGCGATT TATGGACTTT GACTGGTTTC CTGCGTTTAT CACGGGGGCC ACGTCAGGAG CCATTGCTGC GGGAGCCACG GCCAGAGCCA CCACTGCATC GCGCCTGCCG TCCTCTGCTG TGACCCCTCG GGCCCCGCAC CCCAGTCACA CAAGCCAGCC CGTTGCCAAG ACCACGGCAG CCCCCACCAC ACGTCGGCCC CCCACCACTG CCCCCAGCCG TGTGCCCGGA CGTCGGCCCC CGGCCCCCCA GCAGCCTCCA AAGCCCTGTG ACTCACAGCC CTGCTTCCAC GGGGGGACCT GCCAGGACTG GGCATTGGGC GGGGGCTTCA CCTGCAGCTG CCCGGCAGGC AGGGGAGGCG CCGTCTGTGA GAAGGTGCTT GGCGCCCCTG TGCCGGCCTT CGAGGGCCGC TCCTTCCTGG CCTTCCCCAC TCTCCGCGCC TACCACACGC TGCGCCTGGC ACTGGAATTC CGGGCGCTGG AGCCTCAGGG GCTGCTGCTG TACAATGGCA ACGCCCGGGG CAAGGACTTC CTGGCATTGG CGCTGCTAGA TGGCCGCGTG CAGCTCAGGT TTGACACAGG TTCGGGGCCG GCGGTGCTGA CCAGTGCCGT GCCGGTAGAG CCGGGCCAGT GGCACCGCCT GGAGCTGTCC CGGCACTGGC GCCGGGGCAC CCTCTCGGTG GATGGTGAGA CCCCTGTTCT GGGCGAGAGT CCCAGTGGCA CCGACGGCCT CAACCTGGAC ACAGACCTCT TTGTGGGCGG CGTACCCGAG GACCAGGCTG CCGTGGCGCT GGAGCGGACC TTCGTGGGCG CCGGCCTGAG GGGGTGCATC CGTTTGCTGG ACGTCAACAA CCAGCGCCTG GAGCTTGGCA TTGGGCCGGG GGCTGCCACC CGAGGCTCTG GCGTGGGCGA GTGCGGGGAC CACCCCTGCC TGCCCAACCC CTGCCATGGC GGGGCCCCAT GCCAGAACCT GGAGGCTGGA AGGTTCCATT GCCAGTGCCC GCCCGGCCGC GTCGGACCAA CCTGTGCCGA TGAGAAGAGC CCCTGCCAGC CCAACCCCTG CCATGGGGCG GCGCCCTGCC GTGTGCTGCC CGAGGGTGGT GCTCAGTGCG AGTGCCCCCT GGGGCGTGAG GGCACCTTCT GCCAGACAGC CTCGGGGCAG GACGGCTCTG GGCCCTTCCT GGCTGACTTC AACGGCTTCT CCCACCTGGA GCTGAGAGGC CTGCACACCT TTGCACGGGA CCTGGGGGAG AAGATGGCGC TGGAGGTCGT GTTCCTGGCA CGAGGCCCCA GCGGCCTCCT GCTCTACAAC GGGCAGAAGA CGGACGGCAA GGGGGACTTC GTGTCGCTGG CACTGCGGGA CCGCCGCCTG GAGTTCCGCT ACGACCTGGG CAAGGGGGCA GCGGTCATCA GGAGCAGGGA GCCAGTCACC CTGGGAGCCT GGACCAGGGT CTCACTGGAG CGAAACGGCC GCAAGGGTGC CCTGCGTGTG GGCGACGGCC CCCGTGTGTT GGGGGAGTCC CCGGTTCCGC ACACCGTCCT CAACCTGAAG GAGCCGCTCT ACGTAGGGGG CGCTCCCGAC TTCAGCAAGC TGGCCCGTGC TGCTGCCGTG TCCTCTGGCT TCGACGGTGC CATCCAGCTG GTCTCCCTCG GAGGCCGCCA GCTGCTGACC CCGGAGCACG TGCTGCGGCA GGTGGACGTC ACGTCCTTTG CAGGTCACCC CTGCACCCGG GCCTCAGGCC ACCCCTGCCT CAATGGGGCC TCCTGCGTCC CGAGGGAGGC TGCCTATGTG TGCCTGTGTC CCGGGGGATT CTCAGGACCG CACTGCGAGA AGGGGCTGGT GGAGAAGTCA GCGGGGGACG TGGATACCTT GGCCTTTGAC GGGCGGACCT TTGTCGAGTA CCTCAACGCT GTGACCGAGA GCGAGAAGGC ACTGCAGAGC AACCACTTTG AACTGAGCCT GCGCACTGAG GCCACGCAGG GGCTGGTGCT CTGGAGTGGC AAGGCCACGG AGCGGGCAGA CTATGTGGCA CTGGCCATTG TGGACGGGCA CCTGCAACTG AGCTACAACC TGGGCTCCCA GCCCGTGGTG CTGCGTTCCA CCGTGCCCGT CAACACCAAC CGCTGGTTGC GGGTCGTGGC ACATAGGGAG CAGAGGGAAG GTTCCCTGCA GGTGGGCAAT GAGGCCCCTG TGACCGGCTC CTCCCCGCTG GGCGCCACGC AGCTGGACAC TGATGGAGCC CTGTGGCTTG GGGGCCTGCC GGAGCTGCCC GTGGGCCCAG CACTGCCCAA GGCCTACGGC ACAGGCTTTG TGGGCTGCTT GCGGGACGTG GTGGTGGGCC GGCACCCGCT GCACCTGCTG GAGGACGCCG TCACCAAGCC AGAGCTGCGG CCCTGCCCCA CCCCACTCGA GGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGTAG TGAGGGCCCG TTTAAACCCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTC
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