pCMV-Grin2d(mouse-gc-opt)-3×FLAG-Neo
$99.00
In stock
SKU
PL39419
NM_008172.3;GluN2D; NMDAR2D; NR2D
100μg liquid plasmid(Instantly):$120
| name | pCMV-Grin2d(mouse-gc-opt)-3×FLAG-Neo | another name | apCMV-Grin2d(mouse-gc-opt)-3×FLAG-Neo |
| Insert Size (bp) | 3969bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCATGAG GGGAGCAGGG GGACCACGGG GCCCCAGAGG ACCAGCAAAG ATGCTCCTGC TGCTGGCCTT GGCCTGTGCT TCTCCTTTTC CTGAGGAGGT GCCTGGTCCT GGGGCAGCTG GTGGAGGAAC CGGTGGAGCT AGGCCTCTGA ACGTCGCACT TGTGTTCAGC GGCCCCGCCT ATGCTGCCGA AGCAGCCAGG CTCGGGCCTG CAGTCGCCGC AGCTGTCAGG TCTCCCGGGT TGGATGTCAG GCCAGTCGCC CTGGTGCTGA ATGGGTCTGA CCCCAGGAGC CTGGTGCTGC AACTGTGTGA TCTGCTGTCA GGCCTGAGGG TGCACGGGGT TGTCTTTGAG GACGATAGTC GAGCTCCAGC CGTTGCTCCG ATACTGGACT TCCTGAGCGC TCAGACTAGC CTTCCGATCG TCGCAGTGCA CGGAGGTGCC GCTTTGGTGC TCACACCCAA GGAGAAGGGC TCAACGTTCC TCCAGCTCGG AAGCTCTACA GAGCAACAGC TGCAGGTCAT TTTTGAGGTG CTGGAGGAGT ACGACTGGAC ATCCTTTGTG GCAGTGACTA CGCGTGCCCC AGGCCATCGA GCCTTCTTGT CATACATCGA GGTGCTGACT GATGGCAGCC TGGTGGGCTG GGAGCATCGA GGAGCGCTGA CACTGGACCC CGGAGCGGGT GAGGCCGTCC TGGGCGCACA GCTCCGTAGT GTCAGTGCGC AGATCCGCCT GCTCTTCTGC GCCCGCGAGG AGGCAGAGCC TGTTTTCCGG GCGGCAGAAG AGGCTGGTCT CACTGGGCCT GGCTACGTCT GGTTCATGGT GGGACCTCAG CTGGCCGGAG GTGGGGGCTC CGGGGTCCCT GGGGAACCAC TTCTTCTGCC AGGAGGTGCC CCACTGCCTG CTGGGCTGTT TGCAGTGCGC TCTGCTGGCT GGCGTGACGA CTTGGCACGT CGAGTGGCTG CTGGTGTGGC GGTGGTGGCC AGAGGTGCCC AGGCCCTGCT GCGAGACTAT GGCTTCCTGC CTGAGCTGGG CCATGACTGT CGCGCCCAGA ATCGCACCCA CCGCGGGGAG AGTCTGCACA GGTATTTCAT GAACATCACC TGGGATAACC GAGACTACTC CTTCAATGAG GATGGCTTTC TGGTAAACCC GTCACTGGTA GTCATCTCCC TCACCAGAGA CAGGACGTGG GAAGTGGTGG GCAGCTGGGA ACAGCAGACC CTCCGCCTCA AGTACCCTCT ATGGTCCCGC TATGGCCGCT TCCTGCAGCC GGTGGATGAC ACGCAGCACC TCACTGTGGC CACGCTGGAG GAGAGACCTT TTGTCATTGT AGAGCCTGCA GACCCCATCA GCGGCACTTG CATCAGAGAC TCGGTTCCCT GCCGGAGCCA GCTCAACCGT ACCCACAGCC CTCCGCCTGA CGCTCCCCGC CCGGAGAAGA GATGCTGCAA GGGTTTCTGC ATTGACATTT TGAAGAGGCT GGCGCACACC ATCGGTTTCA GCTATGACCT CTACCTGGTT ACCAACGGCA AGCATGGCAA GAAGATCGAT GGCGTCTGGA ATGGCATGAT TGGTGAGGTG TTCTATCAGC GTGCGGACAT GGCCATCGGC TCCCTCACCA TCAATGAGGA GCGGTCAGAG ATCGTGGACT TCTCCGTCCC TTTTGTAGAG ACAGGCATCA GCGTCATGGT GGCACGCAGC AATGGCACTG TGTCCCCCTC TGCCTTCCTC GAGCCCTACA GCCCCGCTGT GTGGGTGATG ATGTTCGTCA TGTGCCTCAC CGTGGTCGCC GTCACAGTTT TCATCTTTGA GTACCTCAGT CCTGTGGGCT ATAACCGAAG CCTGGCCACG GGCAAACGCC CCGGAGGCTC TACCTTCACC ATTGGGAAAT CCATCTGGCT GCTGTGGGCC CTGGTGTTCA ACAACTCCGT GCCAGTGGAG AATCCTCGGG GCACCACCAG CAAGATCATG GTGCTGGTGT GGGCCTTCTT TGCCGTCATC TTTCTTGCCA GCTATACAGC CAATCTGGCT GCCTTCATGA TCCAGGAGGA GTACGTGGAC ACCGTGTCTG GGCTCAGCGA CCGGAAGTTC CAGCGGCCCC AGGAGCAATA CCCACCCCTG AAGTTTGGAA CGGTGCCCAA TGGGTCCACG GAGAAGAATA TCCGAAGCAA CTACCCTGAT ATGCACAGCT ACATGGTGCG ATACAACCAG CCAAGAGTGG AGGAGGCGCT CACTCAGCTC AAGGCAGGGA AACTGGACGC CTTCATCTAT GACGCAGCAG TGCTCAACTA CATGGCCCGA AAGGATGAGG GCTGCAAGCT GGTCACCATC GGCTCAGGCA AGGTCTTCGC CACCACTGGT TATGGCATCG CCCTACACAA GGGCTCCCGC TGGAAGAGGC CCATCGACCT GGCGCTGCTG CAGTTCCTGG GGGACGATGA GATTGAGATG CTGGAGCGGC TGTGGCTTTC AGGGATCTGC CACAACGACA AAATCGAGGT GATGAGCAGC AAGCTGGATA TCGACAACAT GGCAGGTGTC TTCTACATGC TCCTCGTGGC CATGGGCCTC TCCTTGCTGG TCTTCGCCTG GGAACACCTT GTGTACTGGC GACTGCGGCA CTGTCTGGGG CCCACCCACC GCATGGATTT CCTACTGGCC TTCTCCAGGG GTATGTACAG CTGCTGCAGC GCTGAGGCTG CTCCGCCGCC GGCCAAACCC CCGCCACCGC CGCAGCCGCT GCCCAGTCCG GCGTATCCCG CCGCTCGCCC ACCCCCTGGC CCCGCACCCT TCGTGCCCCG AGAGCGCGCA GCCGCCGACC GCTGGCGCCG GGCCAAGGGC ACAGGGCCCC CGGGGGGCGC AGCGCTAGCC GACGGCTTCC ACCGATACTA CGGCCCCATC GAGCCGCAGG GGCTGGGCCT CGGCGAGGCG CGCGCGGCAC CGAGAGGCGC AGCCGGACGC CCACTGTCCC CACCCACCAC ACAGCCCCCA CAGAAGCCAC CACCTTCCTA CTTCGCCATC GTGCGCGAGC AAGAGCCGGC CGAGCCCCCC GCCGGCGCCT TCCCGGGCTT CCCATCTCCG CCCGCTCCAC CTGCCGCCGC AGCCGCCGCC GTCGGGCCGC CACTGTGCCG CCTGGCTTTC GAGGACGAGA GCCCGCCCGC GCCCTCGCGC TGGCCGCGTT CTGACCCCGA GAGCCAGCCG CTGTTGGGTG GGGGCGCGGG CGGCCCGAGC GCTGGGGCCC CGACCGCACC ACCGCCGCGC CGCGCCGCGC CACCACCGTG CGCCTACCTG GACCTCGAGC CTTCGCCTTC GGACTCCGAG GATTCGGAGA GCCTGGGCGG AGCGTCGCTC GGTGGCCTGG AGCCCTGGTG GTTCGCCGAC TTCCCCTACC CGTATGCGGA GCGCCTCGGG CCGCCGCCCG GCCGCTACTG GTCGGTTGAC AAGCTCGGGG GCTGGCGCGC TGGTAGCTGG GACTACCTGC CCCCGCGCGG CGGCCCCGCA TGGCACTGCC GCCACTGCGC CAGCCTGGAG CTGCTACCGC CACCACGCCA TCTCAGCTGC TCGCACGACG GCCTAGACGG TGGCTGGTGG GCGCCTCCGC CTCCACCCTG GGCTGCGGGG CCACCGCCCC GGCGCCGGGC GCGCTGTGGT TGTCCGCGAC CGCACCCGCA CCGCCCCCGG GCTTCGCACC GTGCGCCCGC CGCCGCACCG CACCACCACC GACACAGGCG CGCGGCGGGT GGCTGGGACC TCCCGCCGCC CGCGCCCACC TCGCGTTCGC TGGAGGACCT GAGCTCCTGC CCACGGGCGG CCCCCACGCG CAGGCTCACC GGGCCCTCGC GCCACGCGCG CCGCTGTCCG CACGCTGCGC ATTGGGGGCC GCCCCTGCCC ACCGCATCTC ACCGGAGACA CCGGGGCGGG GACCTGGGCA CACGCAGGGG CTCTGCGCAC TTCTCCAGCC TGGAGTCCGA GGTAGGATCC GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGTAGT GAGGGCCCGA TATCTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCCT GGAAACGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGGTGTTG GGTCGTTTGT TCATAAACGC GGGGTTCGGT CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA TTGGGGCCAA TACGCCCGCG TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT GCCATAGCAG ATCTGCGCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG CATCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGGGGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG ACGGATCGGG AGATCTCCCG ATCCCCTATG GTCGACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATC TGCTCCCTGC TTGTGTGTTG GAGGTCGCTG AGTAGTGCGC GAGCAAAATT TAAGCTACAA CAAGGCAAGG CTTGACCGAC AATTGCATGA AGAATCTGCT TAGG
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