NM_001127244.2;AGM5; HsLRRC8A; LRRC8; SWELL1
100μg liquid plasmid(Instantly):$120
| name | pLV2-TRE3GS-LRRC8A(human)-3×FLAG-TetOne-Puro | another name | apLV2-TRE3GS-LRRC8A(human)-3×FLAG-TetOne-Puro |
| Insert Size (bp) | 2430bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | TRE3GS | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTACTAAGT TTGTAGTACA TATTTAACAA ATACAATTTC TTTAAAAACT ACTAAGTTTG TAGTACATAT TTAACAAATA CAATTTCTTT AAAATGAAAA TAATTCAGAG GAATCACAGG TTTAGAGTAA ATGAAACCAC TAGGTAATTG GCAGTGGTAA TAGGGTATGG GGTGGGAAGT TTGGGATGAT TTTGGTTAGC TTGAGTTATC CAGTTGATCC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT GATCTCCCGA TCCGTCGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCT AGCTAGAGGT CGACGGTAAA ACGCTAGCAA TGTCAAGGCC TCTCACTCTC TGATATTCAT TTCTTTGCAA GTTATAAATA CTGAATAATA AGATGACATG AACTACTACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGGCA AAAAGCAGCT GCTTATATGC AGGATCTGAG GGCTCGCCAC TCCCCAGTCC CGCCCAGGCC ACGCCTCCCT GGAAAGTCCC CAGCGGAAAG TCCCTTGTAG CAAGCTCGAT ATCAGCAGTT CTTGAAGTAC TCCGGATGCA GCTCTCGGGC CACGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AACACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCTGGTG TTCTCTCCTT TATTGGCCTC TTCTACCTTA TCTGGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGACCCCT GGCCCTGGTG TGTAGTTCTG CTAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCT CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACTGCAGAAT TAATTCCAGG CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG CGAAGGCGAA GACGCGGAAG AGGCCGCAGA GCCGGCAGCA GGCCGCGGGA AGGAAGGTCC GCTGGATTGA GGGCCGAAGG GACGTAGCAG AAGGACGTCC CGCGCAGAAT CCAGGTGGCA ACACAGGCGA GCAGCCATGG AAAGGACGTC AGCTTCCCCG ACAACACCAC GGAATTGTCA GTGCCCAACA GCCGAGCCCC TGTCCAGCAG CGGGCAAGGC AGGCGGCGAT GAGTTCCGCC GTGGCAATAG GGAGGGGGAA AGCGAAAGTC CCGGAAAGGA GCTGACAGGT GGTGGCAATG CCCCAACCAG TGGGGGTTGC GTCAGCAAAC ACAGTGCACA CCACGCCACG TTGCCTGACA ACGGGCCACA ACTCCTCATA AAGAGACAGC AACCAGGATT TATACAAGGA GGAGAAAATG AAAGCCATAC GGGAAGCAAT AGCATGATAC AAAGGCATTA AAGCAGCGTA TCCACATAGC GTAAAAGGAG CAACATAGTT AAGAATACCA GTCAATCTTT CACAAATTTT GTAATCCAGA GGTTGATTGT TCCAGACGCG TTCAGGCACC GGGCTTGCGG GTCATGCACC AGGTGCGCGG TCCTTCGGGC ACCTCGACGT CGGCGGTGAC GGTGAAGCCG AGCCGCTCGT AGAAGGGGAG GTTGCGGGGC GCGGAGGTCT CCAGGAAGGC GGGCACCCCG GCGCGCTCGG CCGCCTCCAC TCCGGGGAGC ACGACGGCGC TGCCCAGACC CTTGCCCTGG TGGTCGGGCG AGACGCCGAC GGTGGCCAGG AACCACGCGG GCTCCTTGGG CCGGTGCGGC GCCAGGAGGC CTTCCATCTG TTGCTGCGCG GCCAGCCGGG AACCGCTCAA CTCGGCCATG CGCGGGCCGA TCTCGGCGAA CACCGCCCCC GCTTCGACGC TCTCCGGCGT GGTCCAGACC GCCACCGCGG CGCCGTCGTC CGCGACCCAC ACCTTGCCGA TGTCGAGCCC GACGCGCGTG AGGAAGAGTT CTTGCAGCTC GGTGACCCGC TCGATGTGGC GGTCCGGGTC GACGGTGTGG CGCGTGGCGG GGTAGTCGGC GAACGCGGCG GCGAGGGTGC GTACGGCCCG GGGGACGTCG TCGCGGGTGG CGAGGCGCAC CGTGGGCTTG TACTCGGTCA TGGTCGCGTT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCGCGCGTT TACCCGGGGA GCATGTCAAG GTCAAAATCG TCAAGAGCGT CAGCAGGCAG CATATCAAGG TCAAAGTCGT CAAGGGCATC GGCTGGGAGC ATGTCTAAGT CAAAATCGTC AAGGGCGTCG GTCGGCCCGC CGCTTTCGCA CTTTAGCTGT TTCTCCAGGC CACATATGAT TAGTTCCAGG CCGAAAAGGA AGGCAGGTTC GGCTCCCTGC CGGTCGAACA GCTCAATTGC TTGTTTCAGA AGTGGGGGCA TAGAATCGGT GGTAGGTGTC TCTCTTTCCT CTTTTGCTAC TTGATGCTCC TGTTCCTCCA ATACGCAGCC CAGTGTAAAG TGGCCCACGG CGGACAGAGC GTACAGTGCG TTCTCCAGGG AGAAGCCTTG CTGACACAGG AACGCGAGCT GATTTTCCAG GGTTTCGTAC TGTTTCTCTG TTGGGCGGGT GCCGAGATGC ACTTTAGCCC CGTCGCGATG TGAGAGGAGA GCACAGCGGT ATGACTTGGC GTTGTTCCGC AGAAAGTCTT GCCATGACTC GCCTTCCAGG GGGCAGGAGT GGGTATGATG CCTGTCCAGC ATCTCGATTG GCAGGGCATC GAGCAGGGCC CGCTTGTTCT TCACGTGCCA GTACAGGGTA GGCTGCTCAA CTCCCAGCTT TTGAGCGAGT TTCCTTGTCG TCAGGCCTTC GATACCGACT CCATTGAGTA ATTCCAGAGC AGAGTTTATG ACTTTGCTCT TGTCCAGTCT AGACATGGTA ATTCGATGAT CCCCCTGGGG AGAGAGGTCG GTGATTCGGT CAACGAGGGA GCCGACTGCC GACGTGCGCT CCGGAGGCTT GCAGAATGCG GAACACCGCG CGGGCAGGAA CAGGGCCCAC ACTACCGCCC CACACCCCGC CTCCCGCACC GCCCCTTCCC GGCCGCTGCT CTCGGCGCGC CCTGCTGAGC AGCCGCTATT GGCCACAGCC CATCGCGGTC GGCGCGCTGC CATTGCTCCC TGGCGCTGTC CGTCTGCGAG GGTACTAGTG AGACGTGCGG CTTCCGTTTG TCACGTCCGG CACGCCGCGA ACCGCAAGGA ACCTTCCCGA CTTAGGGGCG GAGCAGGAAG CGTCGCCGGG GGGCCCACAA GGGTAGCGGC GAAGATCCGG GTGACGCTGC GAACGGACGT GAAGAATGTG CGAGACCCAG GGTCGGCGCC GCTGCGTTTC CCGGAACCAC GCCCAGAGCA GCCGCGTCCC TGCGCAAACC CAGGGCTGCC TTGGAAAAGG CGCAACCCCA ACCCCGTGGA ATTATCACCT CGAGTTTACT CCCTATCAGT GATAGAGAAC GTATGAAGAG TTTACTCCCT ATCAGTGATA GAGAACGTAT GCAGACTTTA CTCCCTATCA GTGATAGAGA ACGTATAAGG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGACCAGTT TACTCCCTAT CAGTGATAGA GAACGTATCT ACAGTTTACT CCCTATCAGT GATAGAGAAC GTATATCCAG TTTACTCCCT ATCAGTGATA GAGAACGTAT AAGCTTTGCT TATGTAAACC AGGGCGCCTA TAAAAGAGTG CTGATTTTTT GAGTAAACTT CAATTCCACA ACACTTTTGT CTTATACCAA CTTTCCGTAC CACTTCCTAC CCTCGTAAAG AATTCGCCAC CATGATTCCG GTGACAGAGC TCCGCTACTT TGCGGACACG CAGCCAGCAT ACCGGATCCT GAAGCCGTGG TGGGATGTGT TCACAGACTA CATCTCTATC GTCATGCTGA TGATTGCCGT CTTCGGGGGG ACGCTGCAGG TCACCCAAGA CAAGATGATC TGCCTGCCTT GTAAGTGGGT CACCAAGGAC TCCTGCAATG ATTCGTTCCG GGGCTGGGCA GCCCCTGGCC CGGAGCCCAC CTACCCCAAC TCCACCATTC TGCCGACCCC TGACACGGGC CCCACAGGCA TCAAGTATGA CCTGGACCGG CACCAGTACA ACTACGTGGA CGCTGTGTGC TATGAGAACC GACTGCACTG GTTTGCCAAG TACTTCCCCT ACCTGGTGCT TCTGCACACG CTCATCTTCC TGGCCTGCAG CAACTTCTGG TTCAAATTCC CGCGCACCAG CTCGAAGCTG GAGCACTTTG TGTCTATCCT GCTGAAGTGC TTCGACTCGC CCTGGACCAC GAGGGCCCTG TCGGAGACAG TGGTGGAGGA GAGCGACCCC AAGCCGGCCT TCAGCAAGAT GAATGGGTCC ATGGACAAAA AGTCATCGAC CGTCAGTGAG GACGTGGAGG CCACCGTGCC CATGCTGCAG CGGACCAAGT CACGGATCGA GCAGGGTATC GTGGACCGCT CAGAGACGGG CGTGCTGGAC AAGAAGGAGG GGGAGCAAGC CAAGGCGCTG TTTGAGAAGG TGAAGAAGTT CCGGACCCAT GTGGAGGAGG GGGACATTGT GTACCGCCTC TACATGCGGC AGACCATCAT CAAGGTGATC AAGTTCATCC TCATCATCTG CTACACCGTC TACTACGTGC ACAACATCAA GTTCGACGTG GACTGCACCG TGGACATTGA GAGCCTGACG GGCTACCGCA CCTACCGCTG TGCCCACCCC CTGGCCACAC TCTTCAAGAT CCTGGCGTCC TTCTACATCA GCCTAGTCAT CTTCTACGGC CTCATCTGCA TGTATACACT GTGGTGGATG CTACGGCGCT CCCTCAAGAA GTACTCGTTT GAGTCGATCC GTGAGGAGAG CAGCTACAGC GACATCCCCG ACGTCAAGAA CGACTTCGCC TTCATGCTGC ACCTCATTGA CCAATACGAC CCGCTCTACT CCAAGCGCTT CGCCGTCTTC CTGTCGGAGG TGAGTGAGAA CAAGCTGCGG CAGCTGAACC TCAACAACGA GTGGACGCTG GACAAGCTCC GGCAGCGGCT CACCAAGAAC GCGCAGGACA AGCTGGAGCT GCACCTGTTC ATGCTCAGTG GCATCCCTGA CACTGTGTTT GACCTGGTGG AGCTGGAGGT CCTCAAGCTG GAGCTGATCC CCGACGTGAC CATCCCGCCC AGCATTGCCC AGCTCACGGG CCTCAAGGAG CTGTGGCTCT ACCACACAGC GGCCAAGATT GAAGCGCCCG CGCTGGCCTT CCTGCGTGAG AACCTGCGGG CGCTGCACAT CAAGTTCACC GACATCAAGG AGATCCCGCT GTGGATCTAT AGCCTGAAGA CACTGGAGGA GCTGCACCTG ACGGGCAACC TGAGCGCGGA GAACAACCGC TACATCGTCA TCGACGGGCT GCGGGAGCTC AAACGCCTCA AGGTGCTGCG GCTCAAGAGC AACCTAAGCA AGCTGCCACA GGTGGTCACA GATGTGGGCG TGCACCTGCA GAAGCTGTCC ATCAACAATG AGGGCACCAA GCTCATCGTC CTCAACAGCC TCAAGAAGAT GGCGAACCTG ACTGAGCTGG AGCTGATCCG CTGTGACCTG GAGCGCATCC CCCACTCCAT CTTCAGCCTC CACAACCTGC AGGAGATTGA CCTCAAGGAC AACAACCTCA AGACCATCGA GGAGATCATC AGCTTCCAGC ACCTGCACCG CCTCACCTGC CTTAAGCTGT GGTACAACCA CATCGCCTAC ATCCCCATCC AGATCGGCAA CCTCACCAAC CTGGAGCGCC TCTACCTGAA CCGCAACAAG ATCGAGAAGA TCCCCACCCA GCTCTTCTAC TGCCGCAAGC TGCGCTACCT GGACCTCAGC CACAACAACC TGACCTTCCT CCCTGCCGAC ATCGGCCTCC TGCAGAACCT CCAGAACCTA GCCATCACGG CCAACCGGAT CGAGACGCTC CCTCCGGAGC TCTTCCAGTG CCGGAAGCTG CGGGCCCTGC ACCTGGGCAA CAACGTGCTG CAGTCACTGC CCTCCAGGGT GGGCGAGCTG ACCAACCTGA CGCAGATCGA GCTGCGGGGC AACCGGCTGG AGTGCCTGCC TGTGGAGCTG GGCGAGTGCC CACTGCTCAA GCGCAGCGGC TTGGTGGTGG AGGAGGACCT GTTCAACACA CTGCCACCCG AGGTGAAGGA GCGGCTGTGG AGGGCTGACA AGGAGCAGGC CCTCGAGGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGGGAT CCAGACCACC TCCCCTGCGA GCTAAGCTGG ACAGCCAATG ACGGGTAAGA GAGTGACATT TTTCACTAAC CTAAGACAGG AGGGCCGTCA GAGCTACTGC CTAATCCAAA GACGGGTAAA AGTGATAAAA ATGTATCACT CCAACCTAAG ACAGGCGCAG CTTCCGAGGG ATTTGAGATC CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAA AACTAGAATG CAGTGAAAAA AATGCCTTAT TTGTGAAATT TGTGATGCTA TTGCCTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAAGTCGATA AACTGGATCT CTGCTGTCCC TGTAATAAAC CCGAAAATTT TGAATTTTTG TAATTTGTTT TTGTAGTTCT TTAGTTTGTA TGTCTGTTGC TATTATGTCT ACTATTCTTT CCCCTGCACT GTACCCCCCA ATCCCCCCTT TTCTTTTAAA GGCGATACCG TCGAGATCCG TTCACTAATC GAATGGATCT GTCTCTGTCT CTCTCTCCAC CTTCTTCTTC TATTCCTTCG GGCCTGTCGG GTCCCCTCGG GGTTGGGAGG TGGGTCTGAA ACGATAATGG TGAATATCCC TGCCTAACTC TATTCACTAT AGAAAGTACA GCAAAAACTA TTCTTAAACC TACCAAGCCT CCTACTATCA TTATGAATAA TTTTATATAC CACAGCCAAT TTGTTATGTT AAACCAATTC CACAAACTTG CCCATTTATC TAATTCCAAT AATTCTTGTT CATTCTTTTC TTGCTGGTTT TGCGATTCTT CAATTAAGGA GTGTATTAAG CTTGTGTAAT TGTTAATTTC TCTGTCCCAC TCCATCCAGG TCGTGTGATT CCAAATCTGT TCCAGAGATT TATTACTCCA ACTAGCATTC CAAGGCACAG CAGTGGTGCA AATGAGTTTT CCAGAGCAAC CCCAAATCCC CAGGAGCTGT TGATCCTTTA GGTATCTTTC CACAGCCAGG ATTCTTGCCT GGAGCTGCTT GATGCCCCAG ACTGTGAGTT GCAACAGATG CTGTTGCGCC TCAATAGCCC TCAGCAAATT GTTCTGCTGC TGCACTATAC CAGACAATAA TTGTCTGGCC TGTACCGTCA GCGTCATTGA CGCTGCGCCC ATAGTGCTTC CTGCTGCTCC CAAGAACCCA AGGAACAAAG CTCCTATTCC CACTGCTCTT TTTTCTCTCT GCACCACTCT TCTCTTTGCC TTGGTGGGTG CTACTCCTAA TGGTTCAATT TTTACTACTT TATATTTATA TAATTCACTT CTCCAATTGT CCCTCATATC TCCTCCTCCA GGTCTGAAGA TCAGCGGCCG GCCGCTTGCT GTGCGGTGGT CTTACTTTTG TTTTGCTCTT CCTCTATCTT GTCTAAAGCT TCCTTGGTGT CTTTTATCTC TATCCTTTGA TGCACACAAT AGAGGGTTGC TACTGTATTA TATAATGATC TAAGTTCTTC TGATCCTGTC TGAAGGGATG GTTGTAGCTG TCCCAGTATT TGTCTACAGC CTTCTGATGT TTCTAACAGG CCAGGATTAA CTGCGAATCG TTCTAGCTCC CTGCTTGCCC ATACTATATG TTTTAATTTA TATTTTTTCT TTCCCCCTGG CCTTAACCGA ATTTTTTCCC ATCGCGATCT AATTCTCCCC CGCTTAATAC TGACGCTCTC GCACCCATCT CTCTCCTTCT AGCCTCCGCT AGTCAAAATT TTTGGCGTAC TCACCAGTCG CCGCCCCTCG CCTCTTGCCG TGCGCGCTTC AGCAAGCCGA GTCCTGCGTC GAGAGAGCTC CTCTGGTTTC CCTTTCGCTT TCAAGTCCCT GTTCGGGCGC CACTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GGCAAAAAGC AGCTGCTTAT ATGCAGGATC TGAGGGCTCG CCACTCCCCA GTCCCGCCCA GGCCACGCCT CCCTGGAAAG TCCCCAGCGG AAAGTCCCTT GTAGCAAGCT CGATATCAGC AGTTCTTGAA GTACTCCGGA TGCAGCTCTC GGGCCACGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAACACT TCTCTCTCCG GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCT GGTGTTCTCT CCTTTATTGG CCTCTTCTAC CTTATCTGGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG GATATCTGAC CCCTGGCCCT GGTGTGTAGT TCTGCTAATC AGGGAAGTAG CCTTGTGTGT GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCTTTGGGA GTGAATTAGC CCTTCCA
