pRV-MMLV-CMV-Itga2(mouse)-Puro
$99.00
In stock
SKU
PL39384
100μg liquid plasmid(Instantly):$120
| name | pRV-MMLV-CMV-Itga2(mouse)-Puro | another name | apRV-MMLV-CMV-Itga2(mouse)-Puro |
| Insert Size (bp) | 3537bp | Plasmid host | Mammary cell,retrovirus |
| Plasmid uses | Interfere with silence | Fragment type | shRNA |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | U6 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG CTCAATAAAA GAGCCCACAA CCCCTCACTC GGCGCGCCAG TCTTCCGATA GACTGCGTCG CCCGGGTACC CGTATTCCCA ATAAAGCCTC TTGCTGTTTG CATCCGAATC GTGGTCTCGC TGTTCCTTGG GAGGGTCTCC TCTGAGTGAT TGACTACCCA CGACGGGGGT CTTTCATTTG GGGGCTCGTC CGGGATTTGG AGACCCCTGC CCAGGGACCA CCGACCCACC ACCGGGAGGT AAGCTGGCCA GCAACTTATC TGTGTCTGTC CGATTGTCTA GTGTCTATGT TTGATGTTAT GCGCCTGCGT CTGTACTAGT TAGCTAACTA GCTCTGTATC TGGCGGACCC GTGGTGGAAC TGACGAGTTC TGAACACCCG GCCGCAACCC TGGGAGACGT CCCAGGGACT TTGGGGGCCG TTTTTGTGGC CCGACCTGAG GAAGGGAGTC GATGTGGAAT CCGACCCCGT CAGGATATGT GGTTCTGGTA GGAGACGAGA ACCTAAAACA GTTCCCGCCT CCGTCTGAAT TTTTGCTTTC GGTTTGGAAC CGAAGCCGCG CGTCTTGTCT GCTGCAGCGC TGCAGCATCG TTCTGTGTTG TCTCTGTCTG ACTGTGTTTC TGTATTTGTC TGAAAATTAG GGCCAGACTG TTACCACTCC CTTAAGTTTG ACCTTAGGTC ACTGGAAAGA TGTCGAGCGG ATCGCTCACA ACCAGTCGGT AGATGTCAAG AAGAGACGTT GGGTTACCTT CTGCTCTGCA GAATGGCCAA CCTTTAACGT CGGATGGCCG CGAGACGGCA CCTTTAACCG AGACCTCATC ACCCAGGTTA AGATCAAGGT CTTTTCACCT GGCCCGCATG GACACCCAGA CCAGGTCCCC TACATCGTGA CCTGGGAAGC CTTGGCTTTT GACCCCCCTC CCTGGGTCAA GCCCTTTGTA CACCCTAAGC CTCCGCCTCC TCTTCCTCCA TCCGCCCCGT CTCTCCCCCT TGAACCTCCT CGTTCGACCC CGCCTCGATC CTCCCTTTAT CCAGCCCTCA CTCCTTCTCT AGGCGCCGGA ATTGAAGATC TGGGGGATCG ATCCTCTAGA GTCCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GAATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA GCCTCCGCGG CCGGGAACGG TGCATTGGAA CGCTGCAGGA ATTGATCCGC GGCCGCCACC ATGGGACCGG GACAGGCAGG GGGCGCGCTC CTGCTGCGGC TGCTAATGCT AGTTCAAGGT ATTTTGAATT GTCTGGCGTA TAATGTTGGC CTCCCAGGAG CGAAAATATT TTCCGGGCCT TCAAGTGAAC AGTTTGGGTA TTCAGTACAG CAGCTTACGA ACCCACAAGG CAACTGGCTA CTGGTTGGTT CACCGTGGAG TGGCTTCCCT GAGAACCGAA TGGGAGATGT GTACAAGTGC CCTGTGGACC TACCCACTGC CACCTGTGAA AAGCTGAATC TGCAAAATTC AGCAAGCATC TCAAATGTTA CCGAGATAAA GACCAACATG AGCCTGGGAT TGACCCTCAC CAGGAATCCG GGGACCGGAG GCTTTCTAAC CTGTGGTCCT CTCTGGGCAC ATCAGTGTGG AAATCAGTAT TATGCAACTG GGATCTGTTC TGATGTTAGT CCAGACTTTC AGTTCTTGAC CAGCTTTTCA CCTGCAGTTC AGGCTTGCCC TTCCCTCGTG GATGTTGTAG TTGTATGTGA TGAATCAAAC AGTATTTATC CTTGGGAAGC AGTAAAGAAC TTTTTGGTAA AGTTTGTGAC AGGCCTGGAT ATAGGACCTA AAAAGACACA GGTGGCGTTA ATTCAATATG CCAATGAGCC GAGAATTATA TTTAACTTGA ACGATTTCGA AACCAAAGAG GATATGGTCC AGGCCACATC TGAGACGCGC CAACATGGTG GGGACCTCAC AAACACCTTC AGAGCTATCG AATTCGCAAG AGACTACGCT TATTCACAGA CTTCTGGCGG GCGCCCGGGT GCTACAAAAG TCATGGTAGT TGTGACCGAT GGCGAGTCCC ATGATGGGTC GAAGCTGAAA ACTGTGATCC AGCAATGCAA TGATGACGAG ATACTGAGGT TCGGCATAGC AGTTCTTGGG TATTTAAACA GAAATGCTCT TGATACTAAA AATTTAATCA AAGAAATAAA AGCAATTGCT AGTACTCCAA CCGAGAGATA CTTTTTCAAT GTGGCCGACG AAGCGGCTCT TCTGGAGAAG GCTGGAACTC TAGGGGAGCA AATATTCAGC ATTGAAGGCA CTGTTCAAGG AGGAGACAAC TTCCAGATGG AAATGGCACA AGTTGGATTC AGTGCAGATT ACGCTCCTCA AAATGATATT CTGATGTTAG GTGCAGTGGG GGCTTTTGAC TGGAGTGGAA CTCTTGTCCA GGAGACATCT CACAAGCCCG TGATCTTTCC TAAACAAGCC TTTGACCAGG TTCTGCAGGA TAGAAACCAC AGTTCGTTTT TAGGTTACTC TGTGGCTGCA ATTTCTACTG AAGACGGTGT CCATTTTGTT GCTGGTGCTC CTCGAGCAAA TTATACTGGC CAGATAGTGT TGTACAGCGT TAACAAGCAA GGCAATGTGA CCGTGATTCA GTCTCACAGA GGGGACCAGA TTGGCTCCTA TTTTGGCAGT GTGCTGTGCT CAGTTGATGT AGATAAAGAC ACCATTACAG ACGTGCTCCT GGTAGGTGCT CCTACATACA TGAACGACCT CAAGAAAGAA GAGGGAAAAG TCTACCTATT TACCATCACA AAGGGCATCT TGAATCAGCA CCAGTTTCTT GAAGGGCCAG AAGGCACTGG AAATGCTCGG TTTGGTTCAG CAATTGCAGC CCTGTCAGAC ATCAACATGG ATGGCTTTAA TGATGTGATT GTCGGTTCAC CTGTAGAGAA TGAGAACTCT GGAGCTGTGT ACATTTACAA TGGTCACCAG GGTACCATTC GCACCAAGTA CTCCCAGAAA ATCCTTGGGT CCAATGGAGC CTTTAGGAGA CATCTCCAGT TCTTTGGAAG GTCCCTGGAT GGCTATGGTG ATTTAAATGG GGATTCCATC ACTGATGTAT CCATTGGTGC TTTGGGGCAA GTGATTCAAC TCTGGTCACA AAGCATTGCT GACGTGGCCA TAGAAGCTTT GTTCACTCCA GACAAAATCA CTTTGCTCAA CAAGGATGCT AAGATAACCC TAAAGCTCTG CTTCAGAGCA GAGTTTAGAC CTGCTGGGCA AAACAATCAA GTAGCCATCC TGTTTAACAT GACGCTTGAT GCGGACGGAC ATTCATCCAG GGTAACCTCC AGGGGGGTGT TCAGAGAAAA CAGTGAGCGA TTCTTGCAGA AGAATATGGT GGTAAACGAA GTGCAGAAGT GTTCTGAGCA CCACATTAGC ATACAGAAGC CCTCTGATGT TGTCAACCCT TTGGATCTGC GTGTGGACAT CAGTTTGGAA AACCCAGGCA CTAGCCCTGC CCTCGAAGCC TATTCTGAAA CTGTCAAAGT CTTCAGTATC CCTTTCTATA AGGAGTGTGG CAGCGATGGA ATTTGCATCT CTGACCTGAT CCTGGATGTC CAACAGCTGC CAGCCATTCA AACTCAATCC TTTATTGTCA GTAACCAAAA CAAGAGGTTA ACATTTTCAG TCATCCTGAA AAACAGAGGG GAAAGTGCAT ACAACACTGT GGTCCTGGCC GAGTTTTCTG AGAACTTGTT TTTTGCTTCT TTCTCCATGC CGGTCGATGG AACAGAAGTA ACATGTGAGG TTGGTTCATC GCAGAAATCT GTTACCTGTG ATGTGGGATA CCCTGCCTTA AAGAGTGAAC AGCAGGTGAC TTTTACCATT AACTTCGACT TTAATCTTCA AAACCTCCAG AACCAGGCGG CCATCAATTT CCAAGCCTTC AGTGAGAGCC AAGAAACAAA CAAAGCAGAC AATTCGGTCA GTCTCACGAT TCCTCTCCTG TATGATGCCG AACTTCACTT AACCCGATCC ACCAACATCA ACTTCTATGA GATCTCTTCG GATGAGAACG CTCCTTCTGT CATCAAGAGT GTCGAAGATA TTGGTCCTAA ATTCATCTTC TCCCTCAAGG TAACAGCCGG AAGTGCCCCA GTGAGCATGG CATTGGTGAC TATCCACATT CCTCAGTACA CCAAGGAAAA GAACCCACTC CTGTATCTGA CTGGGATACA AACAGACCAG GCTGGCGACA TCAGCTGCAC AGCAGAAATA AATCCACTCA AGCTACCACA CACCGCCCCT TCTGTATCTT TCAAGAATGA AAACTTCCGG CATACGAAAG AATTGGACTG CAGAACCACT TCCTGCAGTA ACATCACCTG CTGGCTGAAA GACCTTCACA TGAAAGCAGA GTACTTCATC AATGTGACAA CCAGAGTTTG GAACAGGACC TTTGCTGCTT CGACTTTCCA GACTGTGCAG CTGACAGCAG CGGCAGAGAT CGATACACAT AACCCTCAGC TCTTCGTGAT TGAGGAAAAT GCTGTCACGA TTCCCCTCAT GATAATGAAA CCCACGGAGA AAGCAGAAGT ACCGACAGGG GTTATCATAG GCAGCATAAT TGCGGGAATC CTTTTGCTGT TGGCTATGAC TGCAGGTTTG TGGAAGCTCG GCTTCTTCAA AAGACAATAT AAGAAAATGG GCCAAAATCC AGATGAGATG GATGAGACCA CAGAACTCAA CAGCTAAGGA TCCGGAATTC CGCCCCTCTC CCTCCCCCCC CCCTAACGTT ACTGGCCGAA GCCGCTTGGA ATAAGGCCGG TGTGCGTTTG TCTATATGTT ATTTTCCACC ATATTGCCGT CTTTTGGCAA TGTGAGGGCC CGGAAACCTG GCCCTGTCTT CTTGACGAGC ATTCCTAGGG GTCTTTCCCC TCTCGCCAAA GGAATGCAAG GTCTGTTGAA TGTCGTGAAG GAAGCAGTTC CTCTGGAAGC TTCTTGAAGA CAAACAACGT CTGTAGCGAC CCTTTGCAGG CAGCGGAACC CCCCACCTGG CGACAGGTGC CTCTGCGGCC AAAAGCCACG TGTATAAGAT ACACCTGCAA AGGCGGCACA ACCCCAGTGC CACGTTGTGA GTTGGATAGT TGTGGAAAGA GTCAAATGGC TCTCCTCAAG CGTATTCAAC AAGGGGCTGA AGGATGCCCA GAAGGTACCC CATTGTATGG GATCTGATCT GGGGCCTCGG TGCACATGCT TTACATGTGT TTAGTCGAGG TTAAAAAAAC GTCTAGGCCC CCCGAACCAC GGGGACGTGG TTTTCCTTTG AAAAACACGA TGATAAGCTT GCCACAACCC ACAAGGAGAC GACCTTCCAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCC GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ACCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGACG CCCGCCCCAC GACCCGCAGC GCCCGACCGA AAGGAGCGCA CGACCCCATG GCTCCGACCG AAGCCACCCG GGGCGGCCCC GCCGACCCCG CACCCGCCCC CGAGGCCCAC CGACTCTAGT CGAGGGCTGC AGCGCTGCAG AGGCCGAGTG CAGAACTGCT CCAAAGGGAC CTCAAGGCTT TCCGAGGGAC ACTAGGCTGA CTCCATCGAG CCAGTGTAGA GATAAGCTTA TCGATTAGTC CAATTTGTTA AAGACAGGAT ATCAGTGGTC CAGGCTCTAG TTTTGACTCA ACAATATCAC CAGCTGAAGC CTATAGAGTA CGAGCCATAG ATAAAATAAA AGATTTTATT TAGTCTCCAG AAAAAGGGGG GAATGAAAGA CCCCACCTGT AGGTTTGGCA AGCTAGCTTA AGTAACGCCA TTTTGCAAGG CATGGAAAAA TACATAACTG AGAATAGAGA AGTTCAGATC AAGGTCAGGA ACAGATGGAA CAGGGTCGAC CCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC CCCTCACTCG GGGCGCCAGT CCTCCGATTG ACTGAGTCGC CCGGGTACCC GTGTATCCAA TAAACCCTCT TGCAGTTGCA TCCGACTTGT GGTCTCGCTG TTCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC AGCGGGGGTC TTTCATTTGG GGGCTCGTCC GGGATCGGGA GACCCCTGCC CAGGGACCAC CGACCCACCA CCGGGAGGTA AGCTGGCTGC CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCGC AGCCATGACC CAGTCACGTA GCGATAGCGG AGTGTAGATC CGGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTTAC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTG CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA CACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTTCAAG AATTAGCTTG GCCATTGCAT ACGTTGTATC CATATCATAA TATGTACATT TATATTGGCT CATGTCCAAC ATTACCGCCA TGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGT
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