pFUSE2-CL-Kappa(human)
$128.00
In stock
SKU
PL39359
100μg liquid plasmid(Instantly):$149
| name | pFUSE2-CL-Kappa(human) | another name | apFUSE2-CL-Kappa(human) |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression,Antibody expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Blast |
| Selective marker | Blast | Fluorescent labeling | |
| Promoter | EF1a | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGATCTGCGA TCGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CGGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGCTGAAGCT TCGAGGGGCT CGCATCTCTC CTTCACGCGC CCGCCGCCCT ACCTGAGGCC GCCATCCACG CCGGTTGAGT CGCGTTCTGC CGCCTCCCGC CTGTGGTGCC TCCTGAACTG CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA GGTCGAGACC GGGCCTTTGT CCGGCGCTCC CTTGGAGCCT ACCTAGACTC AGCCGGCTCT CCACGCTTTG CCTGACCCTG CTTGCTCAAC TCTACGTCTT TGTTTCGTTT TCTGTTCTGC GCCGTTACAG ATCCAAGCTG TGACCGGCGC CTACCTGAGA TCACCGGTCA CCATGGTGCT GCAGACCCAG GTGTTCATCA GCCTGCTGCT GTGGATCAGC GGCGCCTACG GCGAAGAGCT ACAGAGTGAG CTCTTCAGGA CCGTGGCCGC CCCCAGCGTG TTCATCTTCC CCCCCAGCGA CGAGCAGCTG AAAAGCGGCA CCGCCAGCGT GGTGTGCCTG CTGAACAACT TCTACCCCAG GGAGGCCAAG GTGCAGTGGA AGGTGGACAA CGCCCTGCAG AGCGGCAACA GCCAGGAGAG CGTGACCGAG CAGGACAGCA AGGACAGCAC CTACAGCCTG AGCAGCACCC TGACCCTGAG CAAGGCCGAC TACGAGAAGC ACAAGGTGTA CGCCTGCGAG GTGACCCACC AGGGCCTGAG CAGCCCCGTG ACCAAGAGCT TCAACAGGGG CGAGTGCTGA AGGGAGCTAG CTCGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGAATTA ATTCTAAAAT ACAGCATAGC AAAACTTTAA CCTCCAAATC AAGCCTCTAC TTGAATCCTT TTCTGAGGGA TGAATAAGGC ATAGGCATCA GGGGCTGTTG CCAATGTGCA TTAGCTGTTT GCAGCCTCAC CTTCTTTCAT GGAGTTTAAG ATATAGTGTA TTTTCCCAAG GTTTGAACTA GCTCTTCATT TCTTTATGTT TTAAATGCAC TGACCTCCCA CATTCCCTTT TTAGTAAAAT ATTCAGAAAT AATTTAAATA CATCATTGCA ATGAAAATAA ATGTTTTTTA TTAGGCAGAA TCCAGATGCT CAAGGCCCTT CATAATATCC CCCAGTTTAG TAGTTGGACT TAGGGAACAA AGGAACCTTT AATAGAAATT GGACAGCAAG AAAGCGAGCT TCTAGCTTTA GTTCCTGGTG TACTTGAGGG GGATGAGTTC CTCAATGGTG GTTTTGACCA GCTTGCCATT CATCTCAATG AGCACAAAGC AGTCAGGAGC ATAGTCAGAG ATGAGCTCTC TGCACATGCC ACAGGGGCTG ACCACCCTGA TGGATCTGTC CACCTCATCA GAGTAGGGGT GCCTGACAGC CACAATGGTG TCAAAGTCCT TCTGCCCGTT GCTCACAGCA GACCCAATGG CAATGGCTTC AGCACAGACA GTGACCCTGC CAATGTAGGC CTCAATGTGG ACAGCAGAGA TGATCTCCCC AGTCTTGGTC CTGATGGCCG CCCCGACATG GTGCTTGTTG TCCTCATAGA GCATGGTGAT CTTCTCAGTG GCGACCTCCA CCAGCTCCAG ATCCTGCTGA GAGATGTTGA AGGTCTTCAT GATGGCTCCT CCTGTCAGGA GAGGAAAGAG AAGAAGGTTA GTACAATTGC TATAGTGAGT TGTATTATAC TATGCTTATG ATTAATTGTC AAACTAGGGC TGCAGGGTTC ATAGTGCCAC TTTTCCTGCA CTGCCCCATC TCCTGCCCAC CCTTTCCCAG GCATAGACAG TCAGTGACTT ACCAAACTCA CAGGAGGGAG AAGGCAGAAG CTTGAGACAG ACCCGCGGGA CCGCCGAACT GCGAGGGGAC GTGGCTAGGG CGGCTTCTTT TATGGTGCGC CGGCCCTCGG AGGCAGGGCG CTCGGGGAGG CCTAGCGGCC AATCTGCGGT GGCAGGAGGC GGGGCCGAAG GCCGTGCCTG ACCAATCCGG AGCACATAGG AGTCTCAGCC CCCCGCCCCA AAGCAAGGGG AAGTCACGCG CCTGTAGCGC CAGCGTGTTG TGAAATGGGG GCTTGGGGGG GTTGGGGCCC TGACTAGTCA AAACAAACTC CCATTGACGT CAATGGGGTG GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT ACTGCCAAAA CCGCATCATC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTC CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT TGACGTCAAT AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA ATACTCCACC CATTGACGTC AATGGAAAGT CCCTATTGGC GTTACTATGG GAACATACGT CATTATTGAC GTCAATGGGC GGGGGTCGTT GGGCGGTCAG CCAGGCGGGC CATTTACCGT AAGTTATGTA ACGCCTGCAG GTTAATTAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGGCTA GTTAATTAAC ATTTAAATCA GCGGCCGCAA TAAAATATCT TTATTTTCAT TACATCTGTG TGTTGGTTTT TTGTGTGAAT CGTAACTAAC ATACGCTCTC CATCAAAACA AAACGAAACA AAACAAACTA GCAAAATAGG CTGTCCCCAG TGCAAGTGCA GGTGCCAGAA CATTTCTCTA TCGAA
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