NM_005228.5;ERBB; ERBB1; ERRP; HER1; mENA; NISBD2
100μg liquid plasmid(Instantly):$120
| name | pCMV-EGFR(human)-ECFP-CD274-Neo | another name | apCMV-EGFR(human)-ECFP-CD274-Neo |
| Insert Size (bp) | 3630bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGACTCAG ATCTCGAGCC ACCATGCGAC CCTCCGGGAC GGCCGGGGCA GCGCTCCTGG CGCTGCTGGC TGCGCTCTGC CCGGCGAGTC GGGCTCTGGA GGAAAAGAAA GTTTGCCAAG GCACGAGTAA CAAGCTCACG CAGTTGGGCA CTTTTGAAGA TCATTTTCTC AGCCTCCAGA GGATGTTCAA TAACTGTGAG GTGGTCCTTG GGAATTTGGA AATTACCTAT GTGCAGAGGA ATTATGATCT TTCCTTCTTA AAGACCATCC AGGAGGTGGC TGGTTATGTC CTCATTGCCC TCAACACAGT GGAGCGAATT CCTTTGGAAA ACCTGCAGAT CATCAGAGGA AATATGTACT ACGAAAATTC CTATGCCTTA GCAGTCTTAT CTAACTATGA TGCAAATAAA ACCGGACTGA AGGAGCTGCC CATGAGAAAT TTACAGGAAA TCCTGCATGG CGCCGTGCGG TTCAGCAACA ACCCTGCCCT GTGCAACGTG GAGAGCATCC AGTGGCGGGA CATAGTCAGC AGTGACTTTC TCAGCAACAT GTCGATGGAC TTCCAGAACC ACCTGGGCAG CTGCCAAAAG TGTGATCCAA GCTGTCCCAA TGGGAGCTGC TGGGGTGCAG GAGAGGAGAA CTGCCAGAAA CTGACCAAAA TCATCTGTGC CCAGCAGTGC TCCGGGCGCT GCCGTGGCAA GTCCCCCAGT GACTGCTGCC ACAACCAGTG TGCTGCAGGC TGCACAGGCC CCCGGGAGAG CGACTGCCTG GTCTGCCGCA AATTCCGAGA CGAAGCCACG TGCAAGGACA CCTGCCCCCC ACTCATGCTC TACAACCCCA CCACGTACCA GATGGATGTG AACCCCGAGG GCAAATACAG CTTTGGTGCC ACCTGCGTGA AGAAGTGTCC CCGTAATTAT GTGGTGACAG ATCACGGCTC GTGCGTCCGA GCCTGTGGGG CCGACAGCTA TGAGATGGAG GAAGACGGCG TCCGCAAGTG TAAGAAGTGC GAAGGGCCTT GCCGCAAAGT GTGTAACGGA ATAGGTATTG GTGAATTTAA AGACTCACTC TCCATAAATG CTACGAATAT TAAACACTTC AAAAACTGCA CCTCCATCAG TGGCGATCTC CACATCCTGC CGGTGGCATT TAGGGGTGAC TCCTTCACAC ATACTCCTCC TCTGGATCCA CAGGAACTGG ATATTCTGAA AACCGTAAAG GAAATCACAG GGTTTTTGCT GATTCAGGCT TGGCCTGAAA ACAGGACGGA CCTCCATGCC TTTGAGAACC TAGAAATCAT ACGCGGCAGG ACCAAGCAAC ATGGTCAGTT TTCTCTTGCA GTCGTCAGCC TGAACATAAC ATCCTTGGGA TTACGCTCCC TCAAGGAGAT AAGTGATGGA GATGTGATAA TTTCAGGAAA CAAAAATTTG TGCTATGCAA ATACAATAAA CTGGAAAAAA CTGTTTGGGA CCTCCGGTCA GAAAACCAAA ATTATAAGCA ACAGAGGTGA AAACAGCTGC AAGGCCACAG GCCAGGTCTG CCATGCCTTG TGCTCCCCCG AGGGCTGCTG GGGCCCGGAG CCCAGGGACT GCGTCTCTTG CCGGAATGTC AGCCGAGGCA GGGAATGCGT GGACAAGTGC AACCTTCTGG AGGGTGAGCC AAGGGAGTTT GTGGAGAACT CTGAGTGCAT ACAGTGCCAC CCAGAGTGCC TGCCTCAGGC CATGAACATC ACCTGCACAG GACGGGGACC AGACAACTGT ATCCAGTGTG CCCACTACAT TGACGGCCCC CACTGCGTCA AGACCTGCCC GGCAGGAGTC ATGGGAGAAA ACAACACCCT GGTCTGGAAG TACGCAGACG CCGGCCATGT GTGCCACCTG TGCCATCCAA ACTGCACCTA CGGATGCACT GGGCCAGGTC TTGAAGGCTG TCCAACGAAT GGGCCTAAGA TCCCGTCCAT CGCCACTGGG ATGGTGGGGG CCCTCCTCTT GCTGCTGGTG GTGGCCCTGG GGATCGGCCT CTTCATGCGA AGGCGCCACA TCGTTCGGAA GCGCACGCTG CGGAGGCTGC TGCAGGAGAG GGAGCTTGTG GAGCCTCTTA CACCCAGTGG AGAAGCTCCC AACCAAGCTC TCTTGAGGAT CTTGAAGGAA ACTGAATTCA AAAAGATCAA AGTGCTGGGC TCCGGTGCGT TCGGCACGGT GTATAAGGGA CTCTGGATCC CAGAAGGTGA GAAAGTTAAA ATTCCCGTCG CTATCAAGGA ATTAAGAGAA GCAACATCTC CGAAAGCCAA CAAGGAAATC CTCGATGAAG CCTACGTGAT GGCCAGCGTG GACAACCCCC ACGTGTGCCG CCTGCTGGGC ATCTGCCTCA CCTCCACCGT GCAGCTCATC ACGCAGCTCA TGCCCTTCGG CTGCCTCCTG GACTATGTCC GGGAACACAA AGACAATATT GGCTCCCAGT ACCTGCTCAA CTGGTGTGTG CAGATCGCAA AGGGCATGAA CTACTTGGAG GACCGTCGCT TGGTGCACCG CGACCTGGCA GCCAGGAACG TACTGGTGAA AACACCGCAG CATGTCAAGA TCACAGATTT TGGGCTGGCC AAACTGCTGG GTGCGGAAGA GAAAGAATAC CATGCAGAAG GAGGCAAAGT GCCTATCAAG TGGATGGCAT TGGAATCAAT TTTACACAGA ATCTATACCC ACCAGAGTGA TGTCTGGAGC TACGGGGTGA CTGTTTGGGA GTTGATGACC TTTGGATCCA AGCCATATGA CGGAATCCCT GCCAGCGAGA TCTCCTCCAT CCTGGAGAAA GGAGAACGCC TCCCTCAGCC ACCCATATGT ACCATCGATG TCTACATGAT CATGGTCAAG TGCTGGATGA TAGACGCAGA TAGTCGCCCA AAGTTCCGTG AGTTGATCAT CGAATTCTCC AAAATGGCCC GAGACCCCCA GCGCTACCTT GTCATTCAGG GGGATGAAAG AATGCATTTG CCAAGTCCTA CAGACTCCAA CTTCTACCGT GCCCTGATGG ATGAAGAAGA CATGGACGAC GTGGTGGATG CCGACGAGTA CCTCATCCCA CAGCAGGGCT TCTTCAGCAG CCCCTCCACG TCACGGACTC CCCTCCTGAG CTCTCTGAGT GCAACCAGCA ACAATTCCAC CGTGGCTTGC ATTGATAGAA ATGGGCTGCA AAGCTGTCCC ATCAAGGAAG ACAGCTTCTT GCAGCGATAC AGCTCAGACC CCACAGGCGC CTTGACTGAG GACAGCATAG ACGACACCTT CCTCCCAGTG CCTGAATACA TAAACCAGTC CGTTCCCAAA AGGCCCGCTG GCTCTGTGCA GAATCCTGTC TATCACAATC AGCCTCTGAA CCCCGCGCCC AGCAGAGACC CACACTACCA GGACCCCCAC AGCACTGCAG TGGGCAACCC CGAGTATCTC AACACTGTCC AGCCCACCTG TGTCAACAGC ACATTCGACA GCCCTGCCCA CTGGGCCCAG AAAGGCAGCC ACCAAATTAG CCTGGACAAC CCTGACTACC AGCAGGACTT CTTTCCCAAG GAAGCCAAGC CAAATGGCAT CTTTAAGGGC TCCACAGCTG AAAATGCAGA ATACCTAAGG GTCGCGCCAC AAAGCAGTGA ATTTATTGGA GCAGGAGGTG GAGGTTCAAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTGGG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACATCA GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGGGATC CGGCGCAACA AACTTCTCTC TGCTGAAACA AGCCGGAGAT GTCGAAGAGA ATCCTGGACC GATGAGGATA TTTGCTGTCT TTATATTCAT GACCTACTGG CATTTGCTGA ACGCATTTAC TGTCACGGTT CCCAAGGACC TATATGTGGT AGAGTATGGT AGCAATATGA CAATTGAATG CAAATTCCCA GTAGAAAAAC AATTAGACCT GGCTGCACTA ATTGTCTATT GGGAAATGGA GGATAAGAAC ATTATTCAAT TTGTGCATGG AGAGGAAGAC CTGAAGGTTC AGCATAGTAG CTACAGACAG AGGGCCCGGC TGTTGAAGGA CCAGCTCTCC CTGGGAAATG CTGCACTTCA GATCACAGAT GTGAAATTGC AGGATGCAGG GGTGTACCGC TGCATGATCA GCTATGGTGG TGCCGACTAC AAGCGAATTA CTGTGAAAGT CAATGCCCCA TACAACAAAA TCAACCAAAG AATTTTGGTT GTGGATCCAG TCACCTCTGA ACATGAACTG ACATGTCAGG CTGAGGGCTA CCCCAAGGCC GAAGTCATCT GGACAAGCAG TGACCATCAA GTCCTGAGTG GTAAGACCAC CACCACCAAT TCCAAGAGAG AGGAGAAGCT TTTCAATGTG ACCAGCACAC TGAGAATCAA CACAACAACT AATGAGATTT TCTACTGCAC TTTTAGGAGA TTAGATCCTG AGGAAAACCA TACAGCTGAA TTGGTCATCC CAGAACTACC TCTGGCACAT CCTCCAAATG AAAGGACTCA CTTGGTAATT CTGGGAGCCA TCTTATTATG CCTTGGTGTA GCACTGACAT TCATCTTCCG TTTAAGAAAA GGGAGAATGA TGGATGTGAA AAAATGTGGC ATCCAAGATA CAAACTCAAA GAAGCAAAGT GATACACATT TGGAGGAGAC GTAGCGGCCG CGACTCTAGA TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTAAGGC GTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTC CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCT AGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGT GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA GCCTCAGGTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CATGCAT
