pFUSE-hIgG1
$128.00
In stock
SKU
PL39154
100μg liquid plasmid(Instantly):$149
| name | pFUSE-hIgG1 | another name | apFUSE-hIgG1 |
| Insert Size (bp) | 1074bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Antibody expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Zeo |
| Selective marker | Zeo | Fluorescent labeling | |
| Promoter | EF1a | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGATCTGCGA TCGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CGGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGCTGAAGCT TCGAGGGGCT CGCATCTCTC CTTCACGCGC CCGCCGCCCT ACCTGAGGCC GCCATCCACG CCGGTTGAGT CGCGTTCTGC CGCCTCCCGC CTGTGGTGCC TCCTGAACTG CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA GGTCGAGACC GGGCCTTTGT CCGGCGCTCC CTTGGAGCCT ACCTAGACTC AGCCGGCTCT CCACGCTTTG CCTGACCCTG CTTGCTCAAC TCTACGTCTT TGTTTCGTTT TCTGTTCTGC GCCGTTACAG ATCCAAGCTG TGACCGGCGC CTACCTGAGA TCACCGGCGA AGGAGGGCCA CCATGTACAG GATGCAACTC CTGTCTTGCA TTGCACTAAG TCTTGCACTT GTCACGAATT CGATATCGGC CATGGTTAGA TCTATGGACT GGACCTGGAG GGTGTTCTGC CTGCTGGCCG TGGCCCCCGG CGCCCACAGC GAAGAGCTAC AGAGTGAGCT CTTCGCCAGC ACCAAGGGTC CAAGTGTATT CCCACTCGCT CCAAGCTCTA AATCCACGAG CGGTGGTACG GCAGCCCTCG GATGCCTCGT CAAAGATTAT TTTCCAGAAC CTGTTACAGT ATCTTGGAAT AGCGGCGCGT TGACCAGTGG CGTCCATACA TTTCCCGCGG TATTGCAATC ATCTGGCCTC TATAGTCTTT CATCAGTGGT CACCGTCCCG AGTTCTAGCC TGGGTACACA GACTTACATT TGCAATGTAA ACCATAAGCC AAGCAATACT AAAGTTGACA AGAAGGTCGA GCCCAAAAGC TGTGACAAGA CTCATACCTG CCCGCCCTGC CCAGCCCCCG AACTTCTTGG TGGACCGTCC GTCTTCCTCT TTCCTCCTAA GCCGAAGGAC ACGCTGATGA TCTCAAGGAC TCCCGAAGTT ACATGTGTGG TCGTAGATGT CTCACACGAG GACCCCGAGG TTAAATTCAA TTGGTACGTC GACGGAGTAG AAGTTCATAA CGCCAAAACT AAGCCGAGGG AGGAGCAATA CAATTCAACG TATCGGGTCG TAAGCGTTCT TACCGTCCTC CATCAGGATT GGTTGAACGG TAAAGAATAC AAGTGCAAAG TTAGCAATAA AGCCCTCCCC GCCCCGATAG AAAAGACTAT AAGTAAGGCG AAAGGACAAC CAAGAGAACC CCAAGTTTAC ACTTTGCCCC CCTCACGGGA TGAACTTACA AAAAATCAAG TCTCCCTTAC ATGCCTGGTG AAGGGTTTCT ACCCATCAGA TATCGCAGTG GAATGGGAAT CTAACGGACA ACCAGAAAAT AATTACAAGA CGACGCCGCC AGTCCTCGAC TCTGATGGGT CCTTTTTTTT GTACTCAAAA CTTACTGTGG ATAAAAGTAG GTGGCAACAA GGCAATGTGT TCTCCTGCTC AGTGATGCAT GAGGCCCTTC ACAATCATTA TACCCAGAAA TCATTGAGCC TGAGCCCCGG CAAATGATGA GTGCTAGCTG GCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGAAT TAATTCTAAA ATACAGCATA GCAAAACTTT AACCTCCAAA TCAAGCCTCT ACTTGAATCC TTTTCTGAGG GATGAATAAG GCATAGGCAT CAGGGGCTGT TGCCAATGTG CATTAGCTGT TTGCAGCCTC ACCTTCTTTC ATGGAGTTTA AGATATAGTG TATTTTCCCA AGGTTTGAAC TAGCTCTTCA TTTCTTTATG TTTTAAATGC ACTGACCTCC CACATTCCCT TTTTAGTAAA ATATTCAGAA ATAATTTAAA TACATCATTG CAATGAAAAT AAATGTTTTT TATTAGGCAG AATCCAGATG CTCAAGGCCC TTCATAATAT CCCCCAGTTT AGTAGTTGGA CTTAGGGAAC AAAGGAACCT TTAATAGAAA TTGGACAGCA AGAAAGCGAG CTTCTAGCTT ATCCTCAGTC CTGCTCCTCT GCCACAAAGT GCACGCAGTT GCCGGCCGGG TCGCGCAGGG CGAACTCCCG CCCCCACGGC TGCTCGCCGA TCTCGGTCAT GGCCGGCCCG GAGGCGTCCC GGAAGTTCGT GGACACGACC TCCGACCACT CGGCGTACAG CTCGTCCAGG CCGCGCACCC ACACCCAGGC CAGGGTGTTG TCCGGCACCA CCTGGTCCTG GACCGCGCTG ATGAACAGGG TCACGTCGTC CCGGACCACA CCGGCGAAGT CGTCCTCCAC GAAGTCCCGG GAGAACCCGA GCCGGTCGGT CCAGAACTCG ACCGCTCCGG CGACGTCGCG CGCGGTGAGC ACCGGAACGG CACTGGTCAA CTTGGCCATG ATGGCTCCTC CTGTCAGGAG AGGAAAGAGA AGAAGGTTAG TACAATTGCT ATAGTGAGTT GTATTATACT ATGCAGATAT ACTATGCCAA TGATTAATTG TCAAACTAGG GCTGCAGGGT TCATAGTGCC ACTTTTCCTG CACTGCCCCA TCTCCTGCCC ACCCTTTCCC AGGCATAGAC AGTCAGTGAC TTACCAAACT CACAGGAGGG AGAAGGCAGA AGCTTGAGAC AGACCCGCGG GACCGCCGAA CTGCGAGGGG ACGTGGCTAG GGCGGCTTCT TTTATGGTGC GCCGGCCCTC GGAGGCAGGG CGCTCGGGGA GGCCTAGCGG CCAATCTGCG GTGGCAGGAG GCGGGGCCGA AGGCCGTGCC TGACCAATCC GGAGCACATA GGAGTCTCAG CCCCCCGCCC CAAAGCAAGG GGAAGTCACG CGCCTGTAGC GCCAGCGTGT TGTGAAATGG GGGCTTGGGG GGGTTGGGGC CCTGACTAGT CAAAACAAAC TCCCATTGAC GTCAATGGGG TGGAGACTTG GAAATCCCCG TGAGTCAAAC CGCTATCCAC GCCCATTGAT GTACTGCCAA AACCGCATCA TCATGGTAAT AGCGATGACT AATACGTAGA TGTACTGCCA AGTAGGAAAG TCCCATAAGG TCATGTACTG GGCATAATGC CAGGCGGGCC ATTTACCGTC ATTGACGTCA ATAGGGGGCG TACTTGGCAT ATGATACACT TGATGTACTG CCAAGTGGGC AGTTTACCGT AAATACTCCA CCCATTGACG TCAATGGAAA GTCCCTATTG GCGTTACTAT GGGAACATAC GTCATTATTG ACGTCAATGG GCGGGGGTCG TTGGGCGGTC AGCCAGGCGG GCCATTTACC GTAAGTTATG TAACGCCTGC AGGTTAATTA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGGC TAGTTAATTA ACATTTAAAT CAGCGGCCGC AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGA ATCGTAACTA ACATACGCTC TCCATCAAAA CAAAACGAAA CAAAACAAAC TAGCAAAATA GGCTGTCCCC AGTGCAAGTG CAGGTGCCAG AACATTTCTC TATCGAA
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