pCMV-FLAG-EYFP-CLSPN(human)(1synonymous mutations)-Neo
$113.00
In stock
SKU
PL39057
NM_022111.4
100μg liquid plasmid(Instantly):$134
| name | pCMV-FLAG-EYFP-CLSPN(human)(1synonymous mutations)-Neo | another name | apCMV-FLAG-EYFP-CLSPN(human)(1synonymous mutations)-Neo |
| Insert Size (bp) | 4020bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Yellow | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC AAGCTGATCT ATACATTGAA TCAATATTGG CAATTAGCCA TATTAGTCAT TGGTTATATA GCATAAATCA ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGAC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA ATTGATCTAC CATGGACTAC AAAGACGATG ACGACAAGCT TGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC TTCGGCTACG GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCTAC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGGCGGC CGCGAATTCA ATGACAGGCG AGGTGGGTTC TGAGGTTCAC CTAGAAATCA ATGACCCAAA CGTCATTTCA CAAGAGGAAG CAGATAGTCC TTCAGATAGT GGACAGGGCA GCTATGAAAC AATTGGACCC TTGAGTGAAG GAGATTCAGA TGAAGAGATA TTTGTAAGTA AGAAGTTGAA AAACAGGAAG GTTCTACAAG ACAGTGATTC CGAAACAGAG GACACAAATG CCTCTCCAGA GAAAACTACC TATGACAGTG CCGAGGAGGA AAATAAAGAG AATTTATATG CTGGGAAAAA TACAAAAATC AAAAGGATTT ACAAAACTGT GGCAGACAGT GATGAAAGTT ACATGGAAAA GTCTTTGTAT CAGGAAAATC TTGAAGCGCA AGTGAAACCT TGCTTAGAGC TGAGTCTTCA GTCTGGAAAC TCTACAGACT TTACCACTGA CAGAAAGAGT TCCAAAAAGC ACATACATGA TAAAGAAGGA ACTGCAGGAA AAGCAAAAGT AAAATCAAAA AGAAGACTTG AGAAAGAGGA GAGAAAAATG GAAAAAATTA GACAGCTAAA AAAGAAGGAA ACAAAAAACC AGGAAGATGA TGTAGAACAG CCATTTAATG ACAGTGGCTG TCTTCTTGTG GATAAAGACC TTTTTGAAAC TGGGTTGGAG GATGAAAATA ACTCTCCATT GGAAGATGAA GAGTCATTAG AATCAATAAG AGCAGCTGTA AAAAACAAAG TAAAAAAGCA CAAGAAAAAA GAACCATCTT TGGAGAGTGG GGTCCATTCA TTTGAGGAAG GAAGTGAGTT ATCAAAAGGA ACCACGAGGA AGGAAAGAAA GGCAGCCAGA TTAAGTAAAG AAGCATTAAA ACAACTGCAT AGTGAGACTC AGCGCCTTAT TCGAGAGTCT GCACTGAACC TTCCATATCA TATGCCTGAG AATAAAACCA TTCATGATTT CTTCAAACGT AAACCCCGGC CCACTTGCCA CGGAAATGCC ATGGCACTAT TGAAGTCATC TAAATATCAG TCAAGCCATC ACAAAGAAAT CATAGACACT GCAAATACTA CTGAAATGAA CAGTGATCAC CATAGTAAAG GTTCTGAGCA GACAACAGGT GCAGAAAATG AAGTGGAAAC TAATGCACTC CCTGTAGTTT CAAAGGAAAC CCAGATCATT ACTGGATCAG ATGAGTCTTG CAGGAAGGAT TTGGTAAAAA ATGAAGAGCT AGAAATTCAG GAGAAACAGA AGCAGAGTGA CATTAGACCT TCACCTGGGG ACAGCTCAGT GTTGCAACAG GAATCCAACT TCCTCGGGAA CAATCACAGT GAGGAATGTC AGGTTGGAGG GCTTGTAGCA TTTGAACCTC ATGCCCTGGA GGGTGAAGGC CCCCAAAATC CAGAAGAAAC AGATGAGAAA GTGGAAGAGC CTGAGCAGCA AAATAAATCA TCAGCAGTTG GGCCACCTGA AAAAGTGAGA CGGTTTACTC TGGATAGACT TAAGCAACTG GGAGTAGATG TTTCCATTAA ACCACGGCTA GGTGCTGATG AAGATTCCTT TGTGATACTT GAACCTGAAA CCAACAGAGA ACTGGAAGCC TTGAAGCAGC GTTTCTGGAA GCATGCTAAT CCAGCAGCCA AACCCAGGGC TGGTCAGACA GTGAATGTGA ACGTCATAGT GAAAGACATG GGCACTGATG GAAAGGAAGA GCTAAAAGCA GATGTGGTAC CTGTGACTTT AGCACCTAAG AAGTTGGATG GAGCAAGCCA CACAAAACCA GGTGAAAAGC TTCAGGTGTT AAAAGCTAAA CTGCAAGAAG CAATGAAACT CCGAAGGTTT GAGGAGCGCC AGAAGCGCCA AGCACTGTTT AAATTAGATA ATGAAGATGG GTTTGAGGAA GAGGAGGAGG AAGAGGAAGA AATGACAGAT GAGTCTGAGG AAGATGGAGA AGAGAAGGTA GAGAAAGAAG AGAAAGAGGA AGAACTAGAG GAAGAGGAGG AGAAAGAAGA GGAGGAGGAA GAAGAAGGAA ATCAGGAGAC TGCAGAATTT CTTCTTAGTA GTGAAGAAAT AGAAACAAAA GATGAAAAAG AAATGGATAA AGAAAATAAT GATGGCAGTA GTGAAATTGG CAAGGCAGTT GGCTTCCTCT CTGTTCCCAA GTCTCTCTCA TCAGATTCTA CTTTACTTCT GTTTAAGGAC AGCTCTTCCA AGATGGGTTA CTTTCCTACT GAAGAAAAAT CAGAAACAGA TGAAAACTCA GGCAAGCAGC CTAGCAAACT GGATGAGGAT GATTCATGTT CATTGCTAAC AAAGGAGAGC AGCCACAATA GCAGCTTTGA GCTGATTGGC TCCACGATTC CATCCTATCA GCCTTGCAAC AGACAAACAG GCCGTGGGAC CAGTTTTTTC CCTACAGCAG GAGGATTCAG ATCTCCTTCC CCTGGGCTAT TTCGAGCCAG TTTGGTCAGC TCAGCTTCTA AGAGTTCAGG GAAACTGTCT GAGCCTTCAC TTCCCATAGA GGATTCCCAG GATCTGTATA ACGCCTCCCC AGAGCCTAAG ACACTTTTCC TAGGAGCAGG AGACTTCCAG TTCTGTTTAG AAGATGACAC TCAGAGCCAA CTGTTGGATG CAGATGGGTT CTTAAATGTT AGAAACCACA GGAATCAGTA CCAAGCTTTG AAGCCTCGAT TGCCATTGGC CAGTATGGAT GAGAATGCCA TGGATGCCAA CATGGATGAG CTGTTGGATT TGTGTACTGG AAAGTTCACA TCTCAGGCTG AAAAACATCT ACCCAGGAAG AGTGACAAGA AAGAGAACAT GGAGGAACTT CTGAACCTTT GTTCAGGAAA ATTCACTTCT CAGGATGCCT CCACTCCAGC CTCATCAGAG TTAAATAAAC AGGAGAAGGA GAGCAGCATG GGTGATCCAA TGGAAGAAGC ACTTGCTCTT TGCTCAGGCT CTTTTCCCAC AGACAAGGAA GAGGAAGACG AGGAGGAGGA ATTTGGAGAC TTTCGGCTTG TTTCAAATGA TAATGAGTTT GATAGTGATG AGGATGAACA CAGTGACTCT GGTAATGATC TGGCACTGGA AGACCATGAA GATGATGATG AAGAAGAACT CCTGAAGCGA TCTGAGAAGT TGAAAAGGCA AATGAGGTTG AGGAAATACC TGGAGGATGA GGCAGAGGTG TCAGGAAGTG ATGTGGGAAG CGAAGATGAG TATGATGGGG AAGAAATTGA TGAATATGAA GAGGACGTAA TTGATGAAGT ACTTCCTTCT GATGAGGAAC TGCAGAGTCA AATCAAGAAA ATACACATGA AAACTATGTT GGATGATGAT AAGCGACAGC TACGTTTATA CCAAGAGAGG TACCTTGCTG ATGGGGATCT GCACAGCGAT GGTCCTGGGC GAATGAGGAA GTTTCGATGG AAAAACATAG ATGATGCTTC CCAGATGGAC TTGTTCCACA GAGACTCTGA TGATGATCAG ACTGAAGAAC AGCTTGATGA GTCAGAAGCC AGGTGGAGGA AGGAGCGAAT TGAACGAGAG CAGTGGCTTC GGGACATGGC ACAGCAGGGG AAAATTACAG CTGAAGAAGA AGAAGAAATT GGGGAGGACA GTCAGTTTAT GATACTGGCC AAGAAAGTTA CAGCCAAAGC ACTGCAGAAG AATGCCAGTC GCCCTATGGT TATTCAGGAA TCAAAGTCTT TGCTCAGAAA TCCTTTTGAA GCCATCAGAC CAGGAAGTGC TCAACAGGTG AAGACAGGCT CACTGCTAAA CCAGCCCAAA GCTGTGCTTC AGAAACTGGC TGCTCTCTCT GACCATAACC CCAGTGCTCC TCGAAATTCA AGAAACTTTG TCTTTCATAC ACTTTCTCCT GTCAAGGCTG AGGCGGCAAA GGAATCGTCT AAGTCTCAGG TAAAGAAAAG GGGTCCATCT TTCATGACTT CTCCTTCACC TAAGCACCTC AAAACAGATG ATAGCACTTC AGGATTGACG CGAAGCATCT TCAAATATTT GGAGAGCTAA GGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCTTGGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCTCGAG GAACTGAAAA ACCAGAAAGT TAATTCCCTA TAGTGAGTCG TATTAAATTC GTAATCATGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTT
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