pBS-CMV-GagPol
$113.00
In stock
SKU
PL38964
100μg liquid plasmid(Instantly):$134
| name | pBS-CMV-GagPol | another name | apBS-CMV-GagPol |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Virus | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATAAATCAAT ATTGGCTATT GGCCATTGCA TACGTTGTAT CCATATCATA ATATGTACAT TTATATTGGC TCATGTCCAA CATTACCGCC ATGTTGACAT TGATTATTGA CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC GTTTAGTGAA CCGTCAGATC GCCTGGAGAC GCCATCCACG CTGTTTTGAC CTCCATAGAA GACACCGGGA CCGATCCAGC CTCCGCGGCC CCAAGCTTGG GCATCGTTCT GTGTTGTCTC TGTCTGACTG TGTTTCTGTA TTTGTCTGAA AATATGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG CCCTCACTCC TTCTCTAGGC GCCAAACCTA AACCTCAAGT TCTTTCTGAC AGTGGGGGGC CGCTCATCGA CCTACTTACA GAAGACCCCC CGCCTTATAG GGACCCAAGA CCACCCCCTT CCGACAGGGA CGGAAATGGT GGAGAAGCGA CCCCTGCGGG AGAGGCACCG GACCCCTCCC CAATGGCATC TCGCCTACGT GGGAGACGGG AGCCCCCTGT GGCCGACTCC ACTACCTCGC AGGCATTCCC CCTCCGCGCA GGAGGAAACG GACAGCTTCA ATACTGGCCG TTCTCCTCTT CTGACCTTTA CAACTGGAAA AATAATAACC CTTCTTTTTC TGAAGATCCA GGTAAACTGA CAGCTCTGAT CGAGTCTGTC CTCATCACCC ATCAGCCCAC CTGGGACGAC TGTCAGCAGC TGTTGGGGAC TCTGCTGACC GGAGAAGAAA AACAACGGGT GCTCTTAGAG GCTAGAAAGG CGGTGCGGGG CGATGATGGG CGCCCCACTC AACTGCCCAA TGAAGTCGAT GCCGCTTTTC CCCTCGAGCG CCCAGACTGG GATTACACCA CCCAGGCAGG TAGGAACCAC CTAGTCCACT ATCGCCAGTT GCTCCTAGCG GGTCTCCAAA ACGCGGGCAG AAGCCCCACC AATTTGGCCA AGGTAAAAGG AATAACACAA GGGCCCAATG AGTCTCCCTC GGCCTTCCTA GAGAGACTTA AGGAAGCCTA TCGCAGGTAC ACTCCTTATG ACCCTGAGGA CCCAGGGCAA GAAACTAATG TGTCTATGTC TTTCATTTGG CAGTCTGCCC CAGACATTGG GAGAAAGTTA GAGAGGTTAG AAGATTTAAA AAACAAGACG CTTGGAGATT TGGTTAGAGA GGCAGAAAAG ATCTTTAATA AACGAGAAAC CCCGGAAGAA AGAGAGGAAC GTATCAGGAG AGAAACAGAG GAAAAAGAAG AACGCCGTAG GACAGAGGAT GAGCAGAAAG AGAAAGAAAG AGATCGTAGG AGACATAGAG AGATGAGCAA GCTATTGGCC ACTGTCGTTA GTGGACAGAA ACAGGATAGA CAGGGAGGAG AACGAAGGAG GTCCCAACTC GATCGCGACC AGTGTGCCTA CTGCAAAGAA AAGGGGCACT GGGCTAAAGA TTGTCCCAAG AAACCACGAG GACCTCGGGG ACCAAGACCC CAGACCTCCC TCCTGACCCT AGATGACTAG GGAGGTCAGG GTCAGGAGCC CCCCCCTGAA CCCAGGATAA CCCTCAAAGT CGGGGGGCAA CCCGTCACCT TCCTGGTAGA TACTGGGGCC CAACACTCCG TGCTGACCCA AAATCCTGGA CCCCTAAGTG ATAAGTCTGC CTGGGTCCAA GGGGCTACTG GAGGAAAGCG GTATCGCTGG ACCACGGATC GCAAAGTACA TCTAGCTACC GGTAAGGTCA CCCACTCTTT CCTCCATGTA CCAGACTGTC CCTATCCTCT GTTAGGAAGA GATTTGCTGA CTAAACTAAA AGCCCAAATC CACTTTGAGG GATCAGGAGC TCAGGTTATG GGACCAATGG GGCAGCCCCT GCAAGTGTTG ACCCTAAATA TAGAAGATGA GTATCGGCTA CATGAGACCT CAAAAGAGCC AGATGTTTCT CTAGGGTCCA CATGGCTGTC TGATTTTCCT CAGGCCTGGG CGGAAACCGG GGGCATGGGA CTGGCAGTTC GCCAAGCTCC TCTGATCATA CCTCTGAAAG CAACCTCTAC CCCCGTGTCC ATAAAACAAT ACCCCATGTC ACAAGAAGCC AGACTGGGGA TCAAGCCCCA CATACAGAGA CTGTTGGACC AGGGAATACT GGTACCCTGC CAGTCCCCCT GGAACACGCC CCTGCTACCC GTTAAGAAAC CAGGGACTAA TGATTATAGG CCTGTCCAGG ATCTGAGAGA AGTCAACAAG CGGGTGGAAG ACATCCACCC CACCGTGCCC AACCCTTACA ACCTCTTGAG CGGGCTCCCA CCGTCCCACC AGTGGTACAC TGTGCTTGAT TTAAAGGATG CCTTTTTCTG CCTGAGACTC CACCCCACCA GTCAGCCTCT CTTCGCCTTT GAGTGGAGAG ATCCAGAGAT GGGAATCTCA GGACAATTGA CCTGGACCAG ACTCCCACAG GGTTTCAAAA ACAGTCCCAC CCTGTTTGAT GAGGCACTGC ACAGAGACCT AGCAGACTTC CGGATCCAGC ACCCAGACTT GATCCTGCTA CAGTACGTGG ATGACTTACT GCTGGCCGCC ACTTCTGAGC TAGACTGCCA ACAAGGTACT CGGGCCCTGT TACAAACCCT AGGGAACCTC GGGTATCGGG CCTCGGCCAA GAAAGCCCAA ATTTGCCAGA AACAGGTCAA GTATCTGGGG TATCTTCTAA AAGAGGGTCA GAGATGGCTG ACTGAGGCCA GAAAAGAGAC TGTGATGGGG CAGCCTACTC CGAAGACCCC TCGACAACTA AGGGAGTTCC TAGGGACGGC AGGCTTCTGT CGCCTCTGGA TCCCTGGGTT TGCAGAAATG GCAGCCCCCT TGTACCCTCT CACCAAAACG GGGACTCTGT TTAATTGGGG CCCAGACCAA CAAAAGGCCT ATCAAGAAAT CAAGCAAGCT CTTCTAACTG CCCCAGCCCT GGGGTTGCCA GATTTGACTA AGCCCTTTGA ACTCTTTGTC GACGAGAAGC AGGGCTACGC CAAAGGTGTC CTAACGCAAA AACTGGGACC TTGGCGTCGG CCGGTGGCCT ACCTGTCCAA AAAGCTAGAC CCAGTAGCAG CTGGGTGGCC CCCTTGCCTA CGGATGGTAG CAGCCATTGC CGTACTGACA AAGGATGCAG GCAAGCTAAC CATGGGACAG CCACTAGTCA TTCTGGCCCC CCATGCAGTA GAGGCACTAG TCAAACAACC CCCCGACCGC TGGCTTTCCA ACGCCCGGAT GACTCACTAT CAGGCCTTGC TTTTGGACAC GGACCGGGTC CAGTTCGGAC CGGTGGTAGC CCTGAACCCG GCTACGCTGC TCCCACTGCC TGAGGAAGGG CTGCAACACA ACTGCCTTGA TATCCTGGCC GAAGCCCACG GAACCCGACC CGACCTAACG GACCAGCCGC TCCCAGACGC CGACCACACC TGGTACACGG ATGGAAGCAG TCTCTTACAA GAGGGACAGC GTAAGGCGGG AGCTGCGGTG ACCACCGAGA CCGAGGTAAT CTGGGCTAAA GCCCTGCCAG CCGGGACATC CGCTCAGCGG GCTGAACTGA TAGCACTCAC CCAGGCCCTA AAGATGGCAG AAGGTAAGAA GCTAAATGTT TATACTGATA GCCGTTATGC TTTTGCTACT GCCCATATCC ATGGAGAAAT ATACAGAAGG CGTGGGTTGC TCACATCAGA AGGCAAAGAG ATCAAAAATA AAGACGAGAT CTTGGCCCTA CTAAAAGCCC TCTTTCTGCC CAAAAGACTT AGCATAATCC ATTGTCCAGG ACATCAAAAG GGACACAGCG CCGAGGCTAG AGGCAACCGG ATGGCTGACC AAGCGGCCCG AAAGGCAGCC ATCACAGAGA CTCCAGACAC CTCTACCCTC CTCATAGAAA ATTCATCACC CTACACCTCA GAACATTTTC ATTACACAGT GACTGATATA AAGGACCTAA CCAAGTTGGG GGCCATTTAT GATAAAACAA AGAAGTATTG GGTCTACCAA GGAAAACCTG TGATGCCTGA CCAGTTTACT TTTGAATTAT TAGACTTTCT TCATCAGCTG ACTCACCTCA GCTTCTCAAA AATGAAGGCT CTCCTAGAGA GAAGCCACAG TCCCTACTAC ATGCTGAACC GGGATCGAAC ACTCAAAAAT ATCACTGAGA CCTGCAAAGC TTGTGCACAA GTCAACGCCA GCAAGTCTGC CGTTAAACAG GGAACTAGGG TCCGCGGGCA TCGGCCCGGC ACTCATTGGG AGATCGATTT CACCGAGATA AAGCCCGGAT TGTATGGCTA TAAATATCTT CTAGTTTTTA TAGATACCTT TTCTGGCTGG ATAGAAGCCT TCCCAACCAA GAAAGAAACC GCCAAGGTCG TAACCAAGAA GCTACTAGAG GAGATCTTCC CCAGGTTCGG CATGCCTCAG GTATTGGGAA CTGACAATGG GCCTGCCTTC GTCTCCAAGG TGAGTCAGAC AGTGGCCGAT CTGTTGGGGA TTGATTGGAA ATTACATTGT GCATACAGAC CCCAAAGCTC AGGCCAGGTA GAAAGAATGA ATAGAACCAT CAAGGAGACT TTAACTAAAT TAACGCTTGC AACTGGCTCT AGAGACTGGG TGCTCCTACT CCCCTTAGCC CTGTACCGAG CCCGCAACAC GCCGGGCCCC CATGGCCTCA CCCCATATGA GATCTTATAT GGGGCACCCC CGCCCCTTGT AAACTTCCCT GACCCTGACA TGACAAGAGT TACTAACAGC CCCTCTCTCC AAGCTCACTT ACAGGCTCTC TACTTAGTCC AGCACGAAGT CTGGAGACCT CTGGCGGCAG CCTACCAAGA ACAACTGGAC CGACCGGTGG TACCTCACCC TTACCGAGTC GGCGACACAG TGTGGGTCCG CCGACACCAG ACTAAGAACC TAGAACCTCG CTGGAAAGGA CCTTACACAG TCCTGCTGAC CACCCCCACC GCCCTCAAAG TAGACGGCAT CGCAGCTTGG ATACACGCCG CCCACGTGAA GGCTGCCGAC CCCGGGGGTG GACCATCCTC TAGACTGACA TGGCGCGTTC AACGCTCTCA AAACCCCTTA AAAATAAGGT TGACGCGTCA ACCCGCGAGG CCCCCTAATC CCCTTAATTC TTCTGATGCT CAGAGGGGTC AGTATCGAAT TCCTGCAGCC CCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG CTCGATCCTC TAGAGCGGCC GCCACCGCGG TGGAGCTCCA ATTCGCCCTA TAGTGAGTCG TATTACAATT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGCTTAC AATTTAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT ACCGGGCCCC CCCTCGAGAT CCGGCCATTA GCCATATTAT TCATTGGTTA TATAGC
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